鹿角炭角菌等三种真菌多糖对HIV-RT的作用   总被引：2，自引：0，他引：2  

刘庆洪  罗倩怡  冯昆  王贺祥 《中国食用菌》2004,23(4):29-30

热水抽提和酒精沉淀的方法提取鹿角炭角菌、香栓菌、茶树菇的多糖复合物 ,在反应浓度为 1mg/mL时 ,三种多糖对HIV逆转录酶活性的抑制率分别为 (80 4± 1 2 ) %、 (90 6± 1 7) %和 (84 5± 2 1) %。  相似文献   

Purification and characterization of an antibacterial compound produced by Agrobacterium vitis strain E26 with activity against A. tumefaciens     

H. M. Wang  H. X. Wang  T. B. Ng †  J. Y. Li 《Plant pathology》2003,52(2):134-139

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Diagnosis of Congenital Cardiac Right-to-left Shunts with ^99mTc-macroaggregated Albumin     

Federica Morandi  DVM  MS    Gregory B. Daniel  DVM  MS    Rebecca E. Gompf  DVM  MS    Anne Bahr  DVM  MS 《Veterinary radiology & ultrasound》2004,45(2):97-102

Diagnosing right-to-left congenital cardiac shunts can be difficult. Cardiac catheterization and angiocardiography represent the traditional gold standard for diagnosis, but they are invasive. Nuclear scintigraphy using 99mTc-macroaggregated albumin (MAA) has been employed in humans as an alternate method of diagnosis. This study reviews eight dogs presented for evaluation of a suspect right-to-left cardiac shunt that were examined using 99mTc-MAA. In all, 2-4 mCi (74-148 MBq) of reduced particle 99mTc-MAA were injected IV in a cephalic vein and static images of the whole body, including right and left lateral, dorsal, and ventral views, were acquired for 60 s and stored into a 256 x 256 x 16 matrix. Shunt fractions were calculated. One dog with radiopharmaceutical distribution limited to the lungs did not have a shunt. Seven dogs had distribution of the radiopharmaceutical outside the pulmonary capillary bed, indicating bypassing of the pulmonary capillary circulation due to a right-to-left shunt. Four dogs had 99mTc-MAA within the brain. Three dogs that did not have brain uptake, but instead had a sharp cutoff of radioactivity at the level of the front limbs and neck, were diagnosed with reverse patent ductus arteriosus (PDA). The asymmetric distribution of the radiopharmaceutical is due to the location of the shunt, distal to the brachiocephalic trunk and left subclavian artery. Shunt fractions of dogs with extrapulmonary radioactivity ranged from 40% to 59%. Nuclear scintigraphy with 99mTc-MAA is a quick alternative method of diagnosing right-to-left cardiac shunts that permits quantification of shunt fraction. Distinguishing between reverse PDA and other right-to-left shunts may be possible based on the radiopharmaceutical distribution.  相似文献   

西尼罗热病毒RT-PCR检测方法的建立   总被引：3，自引：0，他引：3  

姜焱张常印  陈国强 《中国动物检疫》2005,22(5):25-27

参考Genebank发表的西尼罗热病毒(West Nile virus，WNV)E糖蛋白基因序列，自行设计合成一对引物，对WNV进行RT—PCR扩增，产物经琼脂糖电泳分析，呈现一条约400bp的条带，将其克隆入pMD18-T—Vector载体中，并进行序列测定，与已发表的WNV基因比较发现，核苷酸的同源性为99．7％，证实为WNV的E基因，通过对样品多次检测，都能扩增出一条约400bp的条带，表明该方法比较稳定。  相似文献   

Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model     

Xiao-ying He  Li-bing Ma  Xiao-ning He  Wan-tong Si  Yue-Mao Zheng 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):145-152

Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated bovine mammary gland epithelial cells (bMGEs) were used as a control group. The results showed that the hTERT-bMGEs group had much higher transgenic efficiency and protein expression than the bMGEs group. Furthermore, the nontransgenic and transgenic hTERT-bMGEs were used as donor cells to evaluate the efficiency of SCNT. There were no significant differences in rates of cleavage or blastocysts or hatched blastocysts of cloned embryos from nontransgenic hTERT-bMGEs at passage 18 and 28 groups (82.8% vs. 81.9%, 28.6% vs. 24.8%, 58.6% vs. 55.3%, respectively) and the transgenic group (80.8%, 26.5% and 53.4%); however, they were significantly higher than the bMGEs group (71.2%, 12.8% and 14.8%), (p < 0.05). We confirmed that hTERT-bMGEs could serve as effective target cells for improving development of somatic cell cloned cattle embryos.  相似文献   

PA-X基因的长度变化对H3N2犬流感病毒复制能力和致病性的影响     

李舜  李桂珍  原耀贤  李康健  马春全  李守军  黄良宗  马骏 《畜牧兽医学报》2023,54(1):272-280

H3N2犬流感病毒(canine influenza virus, CIV)已在中国多地的犬群中流行,是禽流感跨宿主感染并形成新分支的近期案例。研究表明,PA-X基因与甲型流感病毒适应新宿主的能力相关,且其长度能够影响甲型流感病毒的复制及致病能力。为了解PA-X基因的长度变化对H3N2 CIV复制能力及致病力的影响,本研究利用H3N2 CIV的8质粒操作系统,拯救了三株重组H3N2 CIV毒株：PA-X基因表达大小为232个氨基酸多肽的亲本病毒CIV＿PA-X＿232;对PA编码区第191、192位氨基酸的密码子进行改造,PA-X基因不表达蛋白的重组病毒CIV＿PA-X＿Knock;对PA+1编码区第232位氨基酸进行突变,PA-X基因表达大小为252个氨基酸多肽的重组病毒CIV＿PA-X＿252。通过比较3株重组病毒的聚合酶活性,在MDCK细胞中的复制效率及对小鼠致病性的差异,来评价表达不同长度的PA-X基因对H3N2 CIV的影响。结果显示,CIV＿PA-X＿252和CIV＿PA-X＿Knock的聚合酶活性显著(P<0.05)高于CIV＿PA-X＿232,且CIV＿PA-X＿...  相似文献   

Drawing Perspective Projection Free and Easy     

《保鲜与加工》2002,(5):67-70

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用ImageWare实现缺损点云的曲面重构   总被引：1，自引：0，他引：1  

张景涛  卢章平 《农机化研究》2005,(5):90-92

针对有缺损的点云数据,提出了在逆行工程软件ImageWare平台下进行曲面重构的途径;介绍了运用逆向工程技术进行CAD模型设计的操作流程;用平行面截取点云数据,拟合出曲线线条;通过对曲线条的桥接,利用完整的曲线条重构出曲面。  相似文献   

轴流定桨式水轮机抬机事故分析及防止措施   总被引：1，自引：0，他引：1  

蔡付林  季盛林 《中国农村水利水电》1997,(8):10-12

对小型水电站轴流定桨式水轮发电机组的抬机现象进行了分类，从理论上分析了造成抬机现象的原因，并结合试验研究和现场处理经验，提出了防止抬机事故的几项措施。  相似文献   

逆向工程和快速成型技术在农具生产中的应用     

方林宏  樊军  江莉 《农机化研究》2007,(9):189-191

随着现代制造业要求生产加工过程向高度自动化、精度化方向发展,计算机辅助工程在制造业中运用也越来越重要.为此,分析了计算机逆向工程技术和快速成型技术如何应用于羊毛剪刀摆杆模具的制造过程,为以后有关农业器具的生产加工提供了一个新的途径.此项技术不但提高了生产加工过程的自动化、精度化水平,而且有效地提高了生产效率,缩短了产品生产加工周期.  相似文献