Differential Cl−/Salt Tolerance and NaCl‐Induced Alternations of Tissue and Cellular Ion Fluxes in Glycine max,Glycine soja and their Hybrid Seedlings     

X. K. Zhang  Q. H. Zhou  J. H. Cao  B. J. Yu 《Journal of Agronomy and Crop Science》2011,197(5):329-339

The salt‐sensitive Glycine max N23674 cultivar, the salt‐born Glycine soja BB52 population, and their hybrid 4076 strain (F₅) selected for salt tolerance generation by generation were used as the experimental materials in this study. First, the effects of NaCl stress on seed germination, tissue damage, and time‐course ionic absorption and transportation were compared. When qualitatively compared with seed germination appearance in culture dishes, and tissue damages on roots or leaves of seedlings, or quantitatively compared with the relative salt injury rate, the inhibition on N23674 was all the most remarkable. After the exposure of 140 mm NaCl for 1 h, 4 h, 8 h, 12 h, 2 days and 4 days, the content of Cl^? gradually increased in the roots and leaves of seedlings of BB52, 4076 and 23674. Interestingly, the extents of the Cl^? rise in roots of the three experimental soybean materials were BB52 > 4076 > N23674, whereas those in leaves were just on the contrary. Secondly, by using the scanning ion‐selective electrode technique (SIET), fluxes of Na⁺ and Cl^? in roots and protoplasts isolated from roots and leaves were also investigated among the three experimental soybean materials. After 140 mm NaCl stress for 2, 4 and 6 days, and when compared with N23674, slighter net Cl^? influxes were observed in root tissue and protoplasts of roots and leaves of BB52 and 4076 seedlings, especially at the cellular protoplast level. The results indicate that with regard to the ionic effect of NaCl stress, Cl^? was the main determinant salt ion for salt tolerance in G. soja, G. max and their hybrid, and the difference in their Cl^?/salt tolerance is mainly attributed to the capacity of Cl^? restriction to the plant above‐ground parts such as leaves.  相似文献   

野生大豆接种大豆疫霉根腐病菌后超氧化物歧化酶活性变化     

徐鹏飞  鹿文成  靳立梅  吴俊江  李文滨  邱丽娟  常汝镇  王金生  范素杰  李宁辉  王欣  姜良宇  张小明  张淑珍 《作物杂志》2011,27(5):31-35

对大豆疫霉菌胁迫下抗感不同野生大豆根、茎、叶中超氧化物歧化酶(superoxide dis-mutase,SOD)活性的变化进行了初步研究。结果表明:接种大豆疫霉根腐病菌后,抗感野生大豆根、茎、叶中SOD活性在病程的大部分阶段多高于相应对照,且总体而言,抗病野生大豆比感病野生大豆SOD活性较相应对照增幅大。  相似文献   

SSR标记对野生大豆种群遗传结构的研究   总被引：1，自引：1，他引：1  

徐立恒  李向华 《大豆科学》2011,30(1):41-45

使用40对SSR引物分析了8个省区天然野生大豆种群的遗传结构和遗传多样性.结果表明:15个种群共检测到633个等位基因,平均每对引物等位变异基因数平均值15.83个;种群平均Shannon指数(Ⅰ)0.7835,种群平均期望杂合度(He)0.4070,种群平均观察杂合度(Ho)0.0012,平均种群内遗传多样度(Hs)...  相似文献   

我国野生大豆与栽培大豆AFLP指纹图谱研究   总被引：15，自引：3，他引：15  

盖钧镒  喻德跃  吕慧能  贾继增  田清震 《中国农业科学》2001,34(5):480-485

 利用 Mse 和 Eco R 酶切 ,筛选了适宜大豆 AFL P指纹分析的引物组合 ,并利用 17对引物组合 ,建立了我国 92份代表性野生大豆和栽培大豆的 AFL P指纹图谱 ,分析了野生大豆与栽培大豆指纹图谱的特点与差别 ,发现了具有大豆种间特异性的带纹 M- CG/E- CGA- 4、M- CG/E- CGA- 12和 M- CGG/E- GGC- 14 ,并探讨了应用 AFL P指纹图谱鉴别野生大豆与栽培大豆种质的可行性  相似文献   

杉木幼林与大豆立体复合经营模式及其综合效益的研究     

吴祖映  贾金良 《浙江林业科技》1995,15(3):54-59

通过６ａ试验，提出了杉木幼林与大豆立体复合经营模式，其中包括：选择抗旱、耐瘠、高产稳产、适宜杉木幼林立体经营的大豆良种（本试验从７８个品种中筛选出６个优良品种），适时播种（春大豆４月１日～１５日，夏大豆５月１５～２５日），合理的株行距（２０～５０ｃｍ）和每个播种穴合理留苗数（２～４株／穴），用钙镁磷出作基肥（２２５ｋｇ／ｈｍ２），因地制宜施少量氮肥（尿素３７．５～７５．０ｋｇ／ｈｍ２），适宜立体复合经营的杉木幼林应为造林后１～２ａ，林地应为阳坡，豆株与幼树的距离３３ｃｍ以上。杉木—大豆立体经营，不仅能增加粮食和直接经济收入，而且提高了光能利用率，改善林地小气候，减少林地水土流失，改良了土壤，使杉木幼林年生长量比一级提高２０％左右。  相似文献   

野生大豆（Glycinesoja）HSP的研究──Ⅰ．黄河下游和松花江下游地区野生大豆HSP诱导合成的比较研究     

尹田夫  宋海星  王萍  马淑英  李吉平  张让堂 《大豆科学》1996,(3)

本文对黄河下游地区野生大豆“７５８３”和松花江下游地区野生大豆“０１—３２”的发育胚根（Ｓｅｅｄｌｉｎｇｓ）在４０℃下热击分别为２、４、６、８、１０ｈｒ，比较其热击反应（ＨＳＲ）及其热击蛋白（ＨＳＰ）诱导合成的种类、累积动态。研究结果指出黄河下游地区野生大豆对２ｈｒ热击反应较松花江下游地区野生大豆反应迟钝，热击４ｈｒ前者ＨＳＰ种类倍增，且小分子量ＨＳＰ（ＬＭＷＨＳＰ）显著多于后者。ＨＳＰ累积高峰有别，松花江下游野生大豆早于黄河下游野生大豆４个小时，且ＨＳＰ累积量始终高于后者。研究还发现野生大豆存在干热击蛋白（ＨｅａｔＳｈｏｃｋＣｏｇｎａｔｅＰｒｏｔｅｉｎｓ，ＨＳＣＰ）。  相似文献   

野生大豆转录因子GsWRKY57基因的克隆与抗旱性功能分析     

王岩岩  张永兴  郭葳  代文君  周新安  矫永庆  沈欣杰 《中国油料作物学报》2019,(4):524-530

WRKY转录因子是近年来在植物中发现的一类重要转录因子,在植物抗逆相关过程中发挥重要作用。本研究通过对野生大豆转录组数据进行分析,从野生大豆中克隆得到GsWRKY57基因的CDS序列。该基因开放阅读框为903bp,编码300个氨基酸残基,分子量为34.23kD,等电点为5.88。氨基酸序列分析显示该蛋白含有一个WRKY保守结构域,属于III类WRKY转录因子。系统发育树分析表明该蛋白与栽培大豆同源性最高、其次是赤豆、木豆。启动子作用元件分析预测显示该基因可能存在多种非生物胁迫作用元件。组织特异性表达分析表明该基因在大豆叶片中高丰度表达,然后依次是茎、花、荚、根。GsWRKY57基因表达受茉莉酸、水杨酸、脱落酸、干旱等植物逆境诱导。过表达GsWRKY57基因的转基因拟南芥植株相对于野生型耐旱能力增强。  相似文献   

野生大豆与青贮玉米混作对饲草产量及品质的影响     

孙东升  刘成启 《大麦与谷类科学》2019,36(2):38-41

通过在辽宁地区对2个野生大豆品系与青贮玉米进行混作栽培,研究其对饲草产量和营养成分的影响。结果表明:野生大豆与青贮玉米混作可显著提高饲草产量,二者混作收获物青贮后营养成分较玉米单播均有所增加,野生大豆品系LYD140116与青贮玉米在混播密度6.75万株/hm2条件下,饲草产量和品质可达较佳水平。  相似文献   

RFLPs of chloroplast and mitochondrial DNA in wild soybean, Glycine soja, growing in China     

Yoshiya Shimamoto  Hirofumi Fukushi  Jun Abe  Akira Kanazawa  Junyi Gai  Zhong Gao  Donghe Xu 《Genetic Resources and Crop Evolution》1998,45(5):433-439

RFLPs of chloroplast (cp) and mitochondrial (mt) DNA have been used to characterize the cytoplasmic genome of wild soybean, Glycine soja, growing in China. We have found from the RFLPs of that the cytoplasmic genomes of most Chinese wild soybeans have a combination of cpIII with mt-a or a combination of cpII with mt-b accompanied by mtIV or mtV according to our classification. CpII was not observed in combination with mt-a and cpIII was not observed in combination with mt-b. The regional distribution of these two types showed clines with opposite directions. The Yangtze River Valley had the greatest diversity in each of cp and two mt profiles detected in this study and the cytoplasmic genome combining these three profiles showed the highest degree of polymorphism in this region. The Yangtze River Valley may be a center of cytoplasmic diversity of wild soybean and may contain various genetic resources of soybean.  相似文献   

干旱胁迫对野生及栽培大豆幼苗生理特性及抗氧化酶活性的影响     

郑世英  王景平  李士平 《干旱地区农业研究》2014,32(3):35-38

采用盆栽试验,研究了干旱胁迫对野生及栽培大豆生理特性及抗氧化酶活性的影响。即用相对含水量分别为80%、70%、60%、50%、40%、30%的土壤分别栽种野生大豆和栽培大豆,测定其根系活力、细胞膜透性、超氧化物歧化酶（SOD）活性、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性及丙二醛（MDA）含量。结果表明：在同样的土壤相对含水量以及干旱胁迫程度下,相对于栽培大豆,野生大豆表现出高根系活力和抗氧化酶活性以及低细胞膜透性和丙二醛含量。由此可以说明野生大豆的抗干旱能力高于栽培大豆。  相似文献