Expression Analysis of Five Genes in the Gonadal Axis of Small Tail Han Sheep and Sunite Sheep     

ZHANG ZhuangBiao  DI Ran  LIU QiuYue  HU WenPing  WANG XiangYu  TIAN ZhiLong  ZHANG XiaoSheng  ZHANG JinLong  CHU MingXing 《中国农业科学》2018,51(24):4710-4719

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大蒜不定芽的诱导及其增殖系数的调节     

汤青林  王志敏  宋明  王小佳  王平利  向巧彦 《勤云标准版测试》2006,(6)

为获得白皮大蒜组培繁殖体系,以其茎尖为材料,通过BA和NAA不同激素配比试验研究,结果表明:诱导愈伤组织的最适培养基为:MS BA0.2mg/L NAA0.5mg/L,诱导率达60%;诱导不定芽的最适培养基为:MS BA1.0mg/L NAA0.1mg/L,丛生芽繁殖系数高达80%。同时,为建立扩繁最佳体系,分别研究了激素、pH、谷氨酸钠三因素对不定芽增殖系数的调节效果,研究表明:添加0.5mg/L的GA3使增值系数提高到13.8;pH5.8最利于不定芽增值;添加10mg/L谷氨酸钠能在多次继代培养中保持较高的增值系数。  相似文献   

柚木(Tectona grandis)无性系繁育及造林技术     

陈玉桥  梁坤南 《林业调查规划》2006,31(2):106-108

柚木为马鞭草科柚木属植物,是世界上最珍贵的用材树种之一。文章介绍了柚木的生物学特性、无性繁殖即组培技术要点以及造林技术,论述了柚木在我省的推广前景。  相似文献   

月季组织培养过程中的影响因素   总被引：12，自引：0，他引：12  

王正加  高云振  李军萍 《浙江林业科技》2003,23(4):51-56

综述了月季组织培养过程中的影响因素，重点介绍了植物生长调节剂、外植体、培养基和培养条件等对月季试管苗生长的影响。同时分析了月季组培影响因素研究中存在的不足，提出对多因素、复杂因子共同影响行为的研究和探讨是今后月季组培影响因素研究中的重点内容。  相似文献   

Expression and modulation of connective tissue growth factor in renal interstitial fibrosis     

LIU Yan  FAN Jun-ming 《园艺学报》2004,20(9):1725-1728

CTGF, a member of the CCN family of immediate early genes, is a recently discovered profibrotic growth factor, which is involved in many pathophysiologic procedures. CTGF acts as a downstream effector of TGF-β acting on interstitial cells to enhance the progression of fibrotic renal diseases. It has been shown that CTGF gene expression can be induced or blocked by some kinds of cytokine and drugs. It is an interesting candidate target for future intervention strategies of renal interstitial fibrosis.  相似文献   

贮藏中福桔果皮细胞结构的显微观察及果实理化成分测定     

陈文军  洪启征  郑伸坤 《福建农林大学学报(自然科学版)》1991,(2)

贮藏期间福桔果皮细胞始终具有分裂增殖能力,细胞生长的养分主要通过维管束向果肉吸取,因而促进果肉衰老,形成枯水.枯水果实在贮藏早期就发现果皮细胞层和油腔分泌细胞无丝分裂旺盛,双核细胞多,果皮增厚多.严重枯水时,果皮细胞还具细胞核、线粒体、有色体等超微结构,未枯水果实细胞分裂少见.经预贮后贮藏的果实,由于中断了果皮组织与维管束的联系,果皮细胞生长受抑制,果肉水分、养分消耗少,枯水率最低,  相似文献   

In vitro expression and inhibition of procoagulant activity produced by bovine alveolar macrophages and peripheral blood cells     

J. Rashid  D. J. Weiss  S. K. Maheswaran  M. P. Murtaugh 《Veterinary research communications》1996,20(6):519-531

Local and systemic activation of coagulation is frequently associated with bacterial sepsis. The coagulopathy is due, at least in part, to expression of tissue factor (TF) by monocytes and macrophages. The purpose of this study was to evaluate the expression of procoagulant activity by bovine alveolar macrophages, leukocytes and platelets, and to determine the relative potency of three chemical inhibitors of TF expression (pentoxifylline, retinoic acid, and cyclosporin A). Bovine alveolar macrophages were stimulated with lipopolysaccharide (LPS) derived from Pasteurella haemolytica or recombinant bovine tumour nervous factor (TNF) and dose- and time-dependent effects on TF expression were studied. LPS and TNF induced TF expression in alveolar macrophages and LPS treatment of whole blood induced TF expression in mononuclear cells. Neutrophils and platelets also expressed procoagulant activity, but this activity was not inhibited by anti-bovine TF monoclonal antibody. Pentoxifylline (40 mol/L), retinoic acid (0.01 mmol/L) and cyclosporin A (0.08 mol/L) inhibited TF expression when added concurrently with LPS or TNF, but not when added 4 h after stimulation. TF mRNA was not detected in unstimulated alveolar macrophages by Northern blot analysis. In contrast, exposure to LPS or TNF for 6 h induced marked expression of TF mRNA, which was inhibited by treatment with pentoxifylline, retinoic acid and cyclosporin A. Expression of TNF by alveolar macrophages stimulated with LPS was also inhibited by these compounds. Our results indicate that procoagulant activity expressed by alveolar macrophages and monocytes is associated with expression of TF, whereas procoagulant activity expressed by neutrophils and platelets is not. The concentrations of pentoxifylline and retinoic acid necessary for inhibition of TF expression in vitro may not be achievable in vivo owing to their toxic effects. However, the in vitro concentration of cyclosporin A that inhibited TF expression did not exceed the plasma concentration observed in humans, and therefore may be useful for inhibition of TF expression in vivo.Abbreviations BAL bronchoalveolar lavage - LPS lipopolysaccharide - cDNA cloned deoxyribonucleic acid - cAMP cyclic adenosine monophosphate - GAPDH glyceraldehyde phosphate dehydrogenase - mRNA messenger ribonucleic acid - TF tissue factor - TNF tumour necrosis factor - DPBS Dulbecco's phosphate-buffered saline  相似文献   

猕猴桃实生苗组织培养体系建立的研究   总被引：2，自引：0，他引：2  

朱道圩  王静毅  吕宗强 《落叶果树》2005,37(2):5-7

以饱满成熟的猕猴桃种子为起始材料,用 2. 5g·L-1赤霉素处理 5小时,再用次氯酸钠灭菌消毒后,将萌芽的种子接种到MS+蔗糖 20g·L-1 +肌醇 100mg·L-1和 0. 75%琼脂的培养基上,根、茎、叶生长表现良好,初步建立了猕猴桃实生苗组织培养体系。猕猴桃实生苗组织培养同利用茎段、茎尖组织培养一样,培养基中无须附加任何激素。  相似文献   

红千层的离体培养及快速繁殖   总被引：3，自引：0，他引：3  

江洪如  余发新  刘腾云 《园艺学报》2005,32(3):541-544

 研究了红千层离体快繁的若干影响因素。结果表明: 叶片在MS + 6-BA 115 mg·L ^{- 1}+NAA015 mg·L ^{- 1}中的愈伤组织诱导率43% , 并可直接从愈伤组织表面分化出不定芽, 分化率为69.2%; 腋芽启动培养基为MS + 6-BA 1～1.5 mg·L ^{- 1} + NAA 0.5 mg·L ^{- 1} , 萌动率为77%; 继代和增殖培养基为MS + 6-BA 1 mg·L ^{- 1} +NAA 0.25 mg·L ^{- 1} , 平均增殖系数为16.7; 生根壮苗培养基为1 /2MS +NAA 0.25 mg·L ^{- 1} , 生根率96.1%; 试管苗生根培养30～35 d移栽, 成活率最高可达90%以上。  相似文献   

木那格葡萄的组培快繁试验   总被引：2，自引：0，他引：2  

高疆生  张卫芳  欧勇慧 《落叶果树》2003,35(4):4-6

以木那格葡萄的茎尖、单芽茎段为外植体，进行组培快繁试验。蛄果看出，适宜茎尖分化的培养基为B5 BA1．0mg／L IBA 0．1mg／L。适宜单芽茎段一次成苗生长的培养基为B3 BA0．05mg/L IBA0．2mg／L。适宜的生根培齐基为1／2MS IBA 0．2mg／L 蔗糖40g／L。健壮生根的试管苗要待4片叶以上移栽，移栽基质以蛭石较好。时间宜在4—5月份。  相似文献