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目的 探讨栀子苷对酵母多糖（zymosan,Zym）致大鼠肠黏膜屏障损害的作用。方法 通过腹腔注射Zym-石蜡混悬液建立大鼠肠黏膜屏障损害模型,将模型大鼠随机分为模型组、栀子苷组、乌司他丁组,腹腔注射Zym后即刻干预,12 h后重复干预1次。另设正常对照组。造模24 h后测定大鼠血清肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）、白介素-1β（interleukin-1β,IL-1β）、白介素-6（interleukin-6,IL-6）、白介素-10（interleukin-10,IL-10）、D-乳酸（D-lactate,D-LA）、二胺氧化酶（diamine oxidase,DAO）、内毒素（endotoxin,ET）水平,并取小肠进行病理组织学检查。结果 模型组大鼠血清TNF-α、IL-1β、IL-6、IL-10、D-LA、DAO、ET水平较正常对照组显著增高（P<0.01）;病理组织学检查可见黏膜上皮水肿,黏膜上皮细胞排列紊乱,黏膜上皮炎症细胞增多,固有层松散、充血;与模型组比较,栀子苷可降低肠黏膜屏障损害大鼠血清TNF-α、IL-1β、IL-6、D-LA、DAO及ET水平,增加IL-10水平（P<0.05,P<0.01）,减轻肠组织病理性损伤。结论 栀子苷可以减轻Zym导致的大鼠炎症反应,对Zym引起的肠黏膜屏障损害具有保护作用。 相似文献
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Buffalo colostrum caused lysis of unsensitized red blood cells (RBC) from sheep, goats, rabbits and chickens. RBC from cattle and buffalo were resistant to lysis. That lysis was due to the presence of natural antibodies to these RBC was ruled out since there was no reduction in haemolytic titres even after adsorption with the respective RBC. The addition of EGTA to the diluent had no effect on the haemolytic activity. These findings indicate the presence of alternative complement pathway (ACP) activity in buffalo colostrum. The haemolytic activity of buffalo complement for unsensitized rabbit RBC was reduced to very low levels by heating at 50°C for 45 min. Treatment with zymosan also inhibited the haemolytic activity, while inulin had no effect. The maximum activity of ACP occurred in the presence of 4 mmol/L Mg2+ in the diluent. The range of ACP activities in colostrum from buffaloes varied from 4.06 to 8.48 CH50 units/ml. Using a standard system for titrating the classical complement pathway and rabbit red blood cells sensitized with goat haemolysin, the range of complement activity in buffalo colostrum was 4.81–6.77 CH50/ml. 相似文献
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White spot syndrome virus and zymosan A were used to stimulate the Chinese prawn Fenneropenaeus chinensis (Osbeck), and enzyme activities in organs involved in immune defence e.g., lymphoid organ, hepatopancreas and gill, total haemocyte count and mitotic index of haematopoietic tissue (HPT) were measured. The results showed that activities of phenoloxidase (PO), superoxide dismutase (SOD), alkaline phosphatase (ALP) and acid phosphatase (ACP) were higher in lymphoid organ than in gill. The hepatopancreas had no PO activity, its SOD activity was similar to the lymphoid organ, and its ALP and ACP activities were higher than lymphoid organ and gill. Phenoloxidase activity in lymphoid organ was higher than that of the control group at 2 h after injection and lower than control after 24 h. Superoxide dismutase activity in all organs showed an increase. Alkaline phosphatase activity in lymphoid organ and gill was higher than that of the control group at 2 and 24 h after injection respectively. The difference in ACP activity was not significant. After injection with zymosan A, the mitotic index of HPT increased by 1.5‐fold compared with the control, and returned to control level after 48 h. 相似文献
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Neutrophils isolated from the peripheral blood of pigs free of infection withMycoplasma hyopneumoniae were loaded with a fluorescent indicator (Fura-2) for detection of cytosolic free calcium concentration. The kinetics of the intracellular calcium flux were examined after incubation with or without a pathogenic or a non-pathogenic strain ofM. hyopneumoniae. The basal intracellular calcium concentration was not altered by incubation withM. hyopneumoniae. However, the relative increase in cytoplasmic calcium concentration caused by the addition of opsonized zymosan was significantly (p<0.05) higher in neutrophils incubated withM. hyopneumoniae as compared to neutrophils not incubated withM. hyopneumoniae. Additionally, after zymosan stimulation, the intracellular calcium concentration was greater in neutrophils incubated with a pathogenic strain ofM. hyopneumoniae than in those incubated with a non-pathogenic strain. This suggests thatM. hyopneumoniae alters the signal transduction mechanisms in neutrophils and that this alteration may be related to virulence.Abbreviations [Ca]i
intracellular concentration of calcium
- CCU
colour changing units/ml
- Fura-2/AM
pentaacetoxymethyl ester
- PBS
phosphate-buffered saline, pH 7.2
- TNF
tumour necrosis factor 相似文献
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Bitencourt MA Dantas GR Lira DP Barbosa-Filho JM de Miranda GE Santos BV Souto JT 《Marine drugs》2011,9(8):1332-1345
The regulation of the inflammatory response is essential to maintaining homeostasis. Several studies have investigated new drugs that may contribute to avoiding or minimizing excessive inflammatory process. The aim of this study was to evaluate the effect of extracts of green algae Caulerpa mexicana on models inflammation. In mice, the inflammatory peritonitis model is induced by zymosan. Previous treatment of mice with aqueous and methanolic extracts of C. mexicana was able to suppress the cell migration to the peritoneal cavity, in a time-dependent but not in a dose-dependent manner. The treatment of mice with C. mexicana extracts also decreased the xylene-induced ear edema, exerting strong inhibitory leukocyte migration elicited by zymosan into the air pouch. We concluded that administration of the extracts resulted in a reduction of cell migration to different sites as well as a decrease in edema formation induced by chemical irritants. This study demonstrates for the first time the anti-inflammatory effect of aqueous and methanolic extracts from the green marine algae Caulerpa mexicana. 相似文献
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采用流式细胞术对栉孔扇贝(Chlamys farreri)血细胞类群百分比的变化进行了研究,以探讨酵母聚糖和甘氨酸锌对贝类免疫防御影响的规律。体内注射酵母聚糖和甘氨酸锌后,分别于6 h、12 h、24 h、48 h、96 h和144 h测定其血细胞各类群的比例的变化。根据前向角散射光(FSC)和侧向角散射光(SSC)强度的不同,栉孔扇贝血细胞可明显地分为透明细胞、小颗粒细胞和大颗粒细胞3个类群。注射酵母聚糖后,在6 h、12 h、24h和48 h时,实验组透明细胞占总血细胞的比例显著高于对照组,而实验组小颗粒细胞所占比例在6 h、12 h和24 h时显著低于对照组。注射甘氨酸锌后,在12 h、24 h和48 h时,实验组透明细胞所占比例显著高于对照组,而小颗粒细胞所占比例显著低于对照组。说明酵母聚糖和甘氨酸锌对栉孔扇贝血细胞的分群有明显的影响,可显著刺激透明细胞的数量增多,同时颗粒细胞数量减少。 相似文献
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酵母多糖对种鸡生产性能和免疫功能的影响 总被引：1，自引：0，他引：1
旨在研究日粮中添加不同水平酵母多糖对于种鸡生产性能和免疫功能的影响.试验选用288羽45周龄的良风花种鸡,随机分为4个处理组,每个处理组设3个重复,每个重复24只鸡.第Ⅰ组喂以基础日粮,设为对照组;第Ⅱ组添加0.15%的酵母多糖;第Ⅲ组添加0.20%的酵母多糖;第Ⅳ组添加0.25%的酵母多糖.试验前各组生产性能差异不显著,预饲期1周.试验期7周,共8周.结果表明,日粮中添加不同水平的酵母多糖对种鸡生产性能和免疫功能均有显著影响,添加水平为0.15%时产蛋率提高4.56%,种蛋受精率提高3.27%,入孵蛋孵化率提高了7.45%,种鸡体内白介素-2(IL-2)含量较对照组提高7.54%;添加水平为0.20%时产蛋率提高9.16%,种蛋受精率提高4.22%,入孵蛋孵化率提高了5.69%,种鸡体内白介素-2(IL-2)和干扰素-γ(IFN-γ)的含量较对照组也分别提高了9.17%(IL-2)和11.82%(IFN-γ).综合整个试验来说,在种鸡日粮中添加0.20%的酵母多糖为最适水平. 相似文献
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酵母多糖对体外培养小鼠腹腔巨噬细胞免疫功能的增强作用 总被引：4，自引：0，他引：4
目的：探讨酵母多糖对小鼠腹腔巨噬细胞吞噬功能、产生NO和IL-1的影响。方法：将不同剂量的酵母多糖加入体外培养的小鼠腹腔巨噬细胞中，检测巨噬细胞的吞噬能力．取细胞培养上清液根据Griess反应检测NO2的量间接反映巨噬细胞产生NO的生成量，并用MTT比色法检测上清液中IL-1的生成量。结果：酵母多糖可明显促进小鼠腹腔巨噬细胞吞噬中性红的作用，并能促进巨噬细胞产生NO和IL-1，且NO的生成量呈现剂量依赖关系。结论：体外酵母多糖对小鼠腹腔巨噬细胞有免疫增强作用。 相似文献
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采用流式细胞术对栉孔扇贝(Chlamys farreri)血细胞类群百分比的变化进行了研究,以探讨酵母聚糖和甘氨酸锌对贝类免疫防御影响的规律。体内注射酵母聚糖和甘氨酸锌后,分别于6 h、12 h、24 h、48 h、96 h和144 h测定其血细胞各类群的比例的变化。根据前向角散射光(FSC)和侧向角散射光(SSC)强度的不同,栉孔扇贝血细胞可明显地分为透明细胞、小颗粒细胞和大颗粒细胞3个类群。注射酵母聚糖后,在6 h、12 h、24h和48 h时,实验组透明细胞占总血细胞的比例显著高于对照组,而实验组小颗粒细胞所占比例在6 h、12 h和24 h时显著低于对照组。注射甘氨酸锌后,在12 h、24 h和48 h时,实验组透明细胞所占比例显著高于对照组,而小颗粒细胞所占比例显著低于对照组。说明酵母聚糖和甘氨酸锌对栉孔扇贝血细胞的分群有明显的影响,可显著刺激透明细胞的数量增多,同时颗粒细胞数量减少。 相似文献
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Singh, V.K., More, T. and Singh, S., 1997. The effect of activation of granulocytes on enzyme release and hydrogen peroxide and superoxide production in buffaloes. Veterinary Research Communications, 21 (4), 241-247Polymorphonuclear cells kill microorganisms by the stock of antibiotic proteins and peptides stored in their lysosomal granules and have the ability to produce reactive oxygen intermediates (ROI) such as H2O2, O
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, and HOCl. Since the components involved in the microbicidal functions of buffalo (Bos bubalis) polymorphonuclear cells (PMN) have not been characterized, an assessment was made of the levels of various enzymes, the extent of extracellular release of these enzymes, and also their ability to produce H2O2/O
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upon activation with opsonized zymosan (OZ) or lipopolysaccharide (LPS). Using GPC-HPLC, OZ was shown to be a more potent secretagogue than LPS, causing a significantly greater release of low-molecular-weight components. Varying levels of the enzymes (myeloperoxidase, lactate dehydrogenase, acid and alkaline phosphatases, -galactosidase, -D-glucuronidase, elastase and lysozyme) were recorded in the buffalo PMN and both the activators (OZ and LPS) caused significant release of all the enzymes except alkaline phosphatase. Both the activators also caused a significant increase in H2O2/O
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production by the PMN. However, OZ caused a more pronounced activation than LPS. The studies revealed the presence of oxygen-dependent and oxygen-independent microbicidal systems with buffalo PMN, which responded more effectively to zymosan activation. 相似文献
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