Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events     

WU Yu-hua  ;ZHANG Li  ;WU Gang  ;NIE Shu-jing  ;LU Chang-ming 《中国农业科学(英文版)》2014,(9):1865-1876

To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border（RB） and left border（LB） regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head（RB-to-RB） concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.  相似文献   

犬贫血伴有红细胞大小、形态异常疾病的鉴别与诊断     

闫丽 《养殖技术顾问》2014,(11):227-227

犬贫血伴有红细胞大小、形态异常的疾病常见于附红细胞体病、钩虫病、肝硬化、吡哆素缺乏、钴胺素缺乏症、叶酸缺乏症、维生素C缺乏症、铁缺乏症、铜缺乏症、铅中毒、弥散性血管大凝血症等。通过血液涂片直接观察可见，红细胞大小不一，形状异常，甚至细胞内出现异常包涵物。附红细胞体造成大量红细胞破坏，导致骨髓中幼稚红细胞、网织红细胞释放入血，血中网织红细胞增多。  相似文献   

入侵性食叶害虫椰子织蛾的单倍型多样性分析     

李优佳  杨帆  吕宝乾  何荣晓  蔡波 《华南农业大学学报》2020,41(4):76-81

【目的】分析危害棕榈科Palmae植物的一种重要入侵食叶害虫椰子织蛾Opisina arenosella单倍型在原产地和入侵地的分布特点,揭示椰子织蛾入侵我国的虫源信息。【方法】利用线粒体COI基因分析16个地理种群共计172个样本,比较椰子织蛾印度种群和入侵地(中国、马来西亚和泰国)种群的遗传关系。【结果】片段长度为625 bp的172条序列共鉴定出12个单倍型,包含15个变异位点,构成2个明显的单倍型分支,其中一个分支由11个单倍型(IN1～IN11)组成,均来自印度种群,单倍型IN1是6个印度种群的共享单倍型,IN2～IN11为独享单倍型;另一个分支为单倍型HAP,由来自中国、马来西亚和泰国的种群共享;HAP与11个来自印度的单倍型IN1～IN11均存在4个变异位点。【结论】入侵地区的椰子织蛾种群来自同一基因型或者具有相同的入侵源;椰子织蛾种群入侵后受环境选择压力,在新栖息地产生新的突变或杂交。  相似文献   

危险性有害生物椰子织蛾入侵福建的风险性分析     

曹金亮 《安徽农业科学》2014,(25):8578-8580

椰子织蛾是一种近期入侵我国的危险性有害生物。运用国际植物检疫措施实施标准(ISPM)的有害生物风险分析(PRA)程序,建立椰子织蛾传入风险分析评估模型,采用定性分析和多指标综合评估相结合的方法进行分析。结果显示,福建省分布情况,风险值为3;潜在的经济危害性,风险值为2.0;寄主植物的经济重要性,风险值为3;传播扩散的可能性,风险值为1.78;风险管理难度,风险值为2。椰子织蛾的综合风险性值为2.3,符合高度危险的外来有害生物条件,已在我国海南、广东、广西等地区定殖,对福建省的棕榈科植物威胁很大,建议将其列入福建省补充林业检疫性有害生物名单,加强检疫管理、监测和控制工作,严防其传播和扩散危害。  相似文献   

织纳煤田主要煤层煤层气资源量及其可采潜力评价     

史科  吴财芳 《山东饲料》2013,(8):5-6

煤层气资源量是地质综合选区评价的重点,资源量计算的准确与否关系到目标区评价的成败。本文以织纳煤田主要煤层为研究对象,在深入分析煤层厚度、含气量等参数特征的基础上,计算了煤层气资源量,并从含气面积、含气量、资源丰度等关键要素入手,对其可采潜力进行了评价。结果表明:织纳煤田的煤层厚度主要呈现从西向东逐渐变小的趋势,煤层总厚最大能达到20.5m,大部分煤层厚度处于0.8-2.0m。含气量在勺窝、岩脚、茶店、白岩几个地区比较高,在3个主采煤层中,含气量分布特征基本一致。研究区煤层气资源量比较丰富,总资源量达到6367.77×108m3。综合"含气面积、含气量、资源丰度、构造复杂程度"等4个关键参数总体进行分析,煤层气勘探开发最有利区位于化乐地区,白岩、乐治南东翼东段、新华、坐拱等区域是煤层气勘探开发的较有利区。  相似文献   

网络信息组织方法     

刘晓  韩菲  崔晓宁  李树霞 《计算机与农业》2008,(12):55-56

本文介绍了应用于网络信息组织众多方法中比较常用的四种：数字化图书馆、主题网关、书目控制、信息网格。  相似文献   

油茶织蛾生物学特性研究          下载免费PDF全文

李苗苗  舒金平  王井田  华正媛  刘达富  王浩杰  徐天森 《林业科学研究》2015,28(6):900-905

Casmara patrona is one of the main borer pests damaging Camellia oleifera in China. Few reports about this insect were published. The life history, biological characteristics and natural enemies of C. patrona were investigated through field survey and laboratory observation in this study. The results showed that C. patrona had one generation in one year or two years, and overwintered as 3rd-5th instar larva in Zhejiang Province. About 88 percent of larvae began to pupate in early May, and the rest kept feeding till the next year. The adults could be observed from early June to early July, and the peak of emergence occurred in late June. The adults began to oviposit in late June and terminated in early July. The eggs started hatching in early July, and then the larvae bored into the branch to feed. The entrance holes of the larvae distributed predominately in the middle of the eastern and southern part of C. oleifera tree. The larva had five instars, and there were 10-50 days when the larva did not feed between consecutive instars.  相似文献   

网络信息资源组织的方式与方法     

辛伟艳 《山东饲料》2014,(19)

本文先对网络信息资源的特点进行了简要说明,进而深入探讨了网络信息资源组织的方式与方法,以便进行网络信息资源组织的优化研究。  相似文献   

新入侵害虫椰子织蛾飞行能力测定   总被引：3，自引：0，他引：3  

刘向蕊  吕宝乾  金启安  温海波  李朝绪  阎伟  彭正强  冯雨艳  李晓飞 《热带作物学报》2014,35(8):1610-1614

椰子织蛾是危害棕榈科植物的主要害虫之一,2013年入侵中国海南省。为了解椰子织蛾的飞行能力和飞行节律,运用联机飞行磨测定椰子织蛾成虫的飞行能力,结果表明:椰子织蛾单日龄的最大平均飞行距离12 817.47 m(个体单日龄的最大飞行距离为15 338.40 m);椰子织蛾雌、雄虫间的飞行距离差异显著,雄成虫随着日龄增加其飞行能力逐渐增强,雌成虫则随日龄增加飞行能力减弱;总体上,雌、雄椰子织蛾成虫在12:00~24:00时段较活跃,在0:00~12:00不活跃。本研究结果可为椰子织蛾的预警监测与防控提供参考依据。  相似文献   

柞蚕丝棉的拉织     

郭玉清 《农村实用科技》2002,(2):33-33

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