60Co辐射诱变姬松茸突变株J3子实体不同部位的氨基酸分析研究     

黄挺俊  翁伯琦  肖淑霞  王义祥  江枝和 《食用菌学报》2005,12(4):13-16

测定并比较了60Co辐射诱变姬松茸突变株J3与原菌株J1子实体不同部位的氨基酸含量.结果表明,除菌柄和盖皮外,姬松茸突变株J3子实体其它部位的必需氨基酸、儿童氨基酸、硫氨酸、支链氨基酸、鲜味氨基酸、芳香族氨基酸和甜味氨基酸均高于原菌株J1.姬松茸突变株J3子实体中谷氨酸含量占氨基酸总量的比例分别比原菌株J1、α-酪蛋白和卵清蛋白提高34.88%、56.68%和122.32%.  相似文献   

Bcl-2 antisense oligodeoxynucleotide increases the sensitivity of HL60 and K562 cells to daunorubicin     

LEI Xiao-yong  ZHANG Huan  HE Dong-mei 《园艺学报》2003,19(2):194-197

AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P＜0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P＜0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner.  相似文献   

The mechanism of apoptosis induced by homoharringtonine in HL-60 cells     

CHEN Chun-yan  JIA Ji-hui  PAN Xiang-lin  ZHANG Qi  WANG Juan-dong  ZHOU Ya-bin 《园艺学报》2004,20(7):1183-1186

AIM: To study the signal transduction pathway of apoptosis initiation induced by homoharringtonine in HL-60 cells. METHODS: After establishing the model of apoptosis initiation induced by homoharringtonine in HL-60 cells, at the point of apoptosis initiation, molecular caspase-3, Bcl-2, Bax and Fas/FasL were measured with flow cytometry and transmission electron microscope. ERK2 and P38 expression in HL-60 cells were detected by using immunohistochemistry. RESULTS: The model of apoptosis initiation induced by homoharringtonine was established in HL-60 cells. At the point of apoptosis initiation, upregulation of caspase-3 and decrease in Bcl-2/Bax were observed. However, the expression of Fas/FasL did not significantly change. ERK2 expression decreased and P38 expression increased. CONCLUSIONS: Caspase-3, Bcl-2, Bax and mitogen activated protein kinase pathways were involved in signal transduction of apoptosis initiation induced by homoharringtonine in HL-60 cells.  相似文献   

Screening and Identification of Candidate Proteins Interacting with BmHSP60 in the Silkworm (Bombyx mori)     

DONG ZhanQi  JIANG YaMing  PAN MinHui 《中国农业科学》2019,52(2):376-384

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Cytokine profiles in peripheral blood mononuclear cells from patients with cystic echinococcosis     

《Comparative immunology, microbiology and infectious diseases》2020

IntroductionCystic echinococcosis (CE) is a chronic zoonotic disease caused by the larval stage of Echinococcus granulosus (E. granulosus), which affects domestic and wild carnivores as the definitive host and ungulates as intermediate hosts. In intermediate hosts, both Th1 and Th2 cells are involved in the immune responses to an echinoccocal infection. This study aimed to investigate production of IL-4, IL-10, and IFN-γ cytokines in peripheral blood mononuclear cells (PBMCs) of CE patients before and after surgical treatment.MethodsTo evaluate cytokine production in response to E. granulosus antigens, we investigated IL-4, IL-10, and IFN-γ production in PBMCs of 20 CE patients in response to hydatid cyst fluid antigen (HCF-Ag) before and after surgical treatment using ELISA.ResultsThe mean IL-4 production from HCF-Ag stimulated PBMCs was significantly decreased (p < 0.05), while IFN-γ was significantly increased in HCF-Ag stimulated PBMCs in patients after surgery (p = 0.005).Furthermore, our results showed that there is no significant difference between IL-10 production in patients before and after treatment (p = 0.562).ConclusionsOur data Indicated production of IL-4 in cultured PBMCs of CE patients stimulated with HCF-Ag was decreased significantly. While, production of IFN-γ was increased significantly in responses to HCF Ag after surgery. We concluded that the evaluation of IL-4 and IFN-γ in HCF-Ag stimulated PBMCs of CE patients should be considered as a useful marker in the follow up of patients with cystic echinococcosis.  相似文献   

Irradiation Effect in C60 Films Induced by 120 keV H+ Ions     

ZHU Sheng -xing  YAO Jiang -hong 《保鲜与加工》2002,(7):57-59

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牛磺酸、γ-氨基丁酸对鲫抗缺氧能力的影响   总被引：8，自引：0，他引：8  

魏智清  杨涓  赵红雪  马瑛 《淡水渔业》2006,36(1):7-10

研究牛磺酸、γ-氨基丁酸对鲫抗缺氧能力的影响,结果表明质量分数5‰、7‰牛磺酸水溶液对鲫抗缺氧能力有显著(P<0.05)和极显著作用(P<0.01),质量分数3‰γ-氨基丁酸水溶液对鲫抗缺氧有显著作用(P<0.05)。实验证明,γ-氨基丁酸、牛磺酸均可提高鲫的抗缺氧能力。  相似文献   

利用高巧(60%吡虫啉悬浮种衣剂)防治小豆蚜虫效果研究     

王洪皓  乔辉  何伟锋  徐敏  赵秋 《园艺与种苗》2020,(3):50-52

[目的]探讨小豆蚜虫防治新技术,比较研究种衣剂拌种的防治效果。[方法]选用高巧(60%吡虫啉悬浮种衣剂)进行播前拌种试验,采用50 mL水分别加入高巧2、4、6、8 mL拌种1.0kg的处理方法,播种后比较其出苗率及幼苗的发育情况、蚜虫防治效果、小豆百粒重、产量及增产率。[结果]高巧拌种不会影响小豆出苗,可有效防治小豆蚜虫,但防效随时间延长而降低。防效保持在85%以上,可显著提高小豆百粒重和产量。[结论]该技术可在小豆生产中进行推广应用。  相似文献   

60Co-γ辐射对大花紫薇叶绿素荧光特性的影响     

牛贺雨  刘丹  彭梦婕  徐晴晴  王献 《东北林业大学学报》2020,(1):23-28

为探究60Co-γ射线辐射处理种子对大花紫薇生长发育中光合效率的影响,以大花紫薇种子为试验材料,以不同60Co-γ射线辐射剂量(0、20、60、100、140、180 Gy)进行辐射处理,测量不同处理生长发育过程中叶绿素及光合指标的变化,研究其对紫薇光合系统的影响。结果表明:20、60 Gy处理较对照和其他处理叶绿素质量分数更高,更有利于叶片进行光合作用;60、100 Gy处理较对照和其他处理潜在光合速率和最大光合效率更好;各时期光合性能指数与综合性能指数均呈现先上升后下降的趋势,但随时间的增加整体呈现下降的趋势,60 Gy较其他处理性能指数高。由此得出,60 Gy辐射处理有利于大花紫薇光合色素的产生与积累,较好的增加电子传递效率,有利于光合系统的运转和能量的积累。  相似文献   

60 Co-γ射线辐照处理对4种药用植物种子萌发的影响     

吴萍  郭俊霞  王晓宇  李青苗  张松林 《种子》2021,(1):6-10

用不同剂量60Co-γ射线对益母草、蒲公英、车前、紫苏种子进行辐照处理,测定这4种药用植物种子的发芽率、发芽势、发芽指数等指标,了解60 Co-γ射线辐照对其种子萌发的影响,为这4种药用植物的新品种选育提供参考.结果表明,60 Co-γ射线对4种药用植物种子具有较强的诱发突变能力,辐照剂量与种子发芽率、发芽势、发芽指数...  相似文献