排序方式： 共有21条查询结果，搜索用时 31 毫秒
1.
枇杷果实发育过程中山梨醇含量及相关酶活性的变化 总被引：1，自引：0，他引：1
以果型和品质差异较大的黄肉枇杷(Eriobotrya japonica Lindl.)‘解放钟’和白肉枇杷‘白梨’的果实为试材,测定果实发育过程中山梨醇、果糖、葡萄糖含量及与山梨醇代谢相关酶--山梨醇脱氢酶(Sorbitol dehydrogenase,SDH)和山梨醇氧化酶(Sorbitol oxidase,SOX)活性的变化,并探讨山梨醇代谢与枇杷果实生长发育和品质形成的关系。结果表明:2个枇杷品种果实中山梨醇含量均随果实发育进程呈下降趋势,且‘白梨’枇杷果实中山梨醇含量高于‘解放钟’。在花后35~80 d,2个枇杷品种幼果中葡萄糖和果糖含量均较低且相对稳定,而花后95~140 d果实中葡萄糖和果糖含量逐渐上升,并在花后125~140 d果实成熟期急剧增加而达到峰值;果实中葡萄糖含量在2个品种间差异不显著(p0.05),花后125~140 d的果实成熟期,‘白梨’果实中果糖含量显著高于‘解放钟’(p0.05)。果实发育过程中SDH和SOX的活性均呈先降后升的变化趋势。说明2个枇杷品种果实发育过程中山梨醇、果糖、葡萄糖含量及SDH和山SOX的活性表现出相似的变化规律,但果实山梨醇含量与SDH和SOX的活性在果实膨大期均存在显著差异(p0.05),这可能是黄肉枇杷与白肉枇杷品质差异的关键。 相似文献
2.
Advanced glycation end products and sorbitol in blood from differently compensated diabetic dogs 总被引：1，自引：0，他引：1
Comazzi S Bertazzolo W Bonfanti U Spagnolo V Sartorelli P 《Research in veterinary science》2008,84(3):341-346
Canine diabetes mellitus (DM) is a common metabolic disorder with long term complications, most of which are caused by glycosylation of structural proteins, decreases in antioxidant concentrations, altered osmotic balance and hypoxia due to impaired oxygen transport. Previous studies have demonstrated that under hyperglycemic conditions canine erythrocytes undergo swelling, probably due to activation of the polyol pathway. The present work aimed to assess the plasma concentration of advanced glycation end (AGE) products, stable Amadori-products generated by non-enzymatic glycosylation of proteins and the intracellular concentration of sorbitol, produced by the activation of polyol pathway in 34 blood samples from diabetic dogs and in 14 controls. AGE products were significantly higher (p<0.01) in plasma from diabetic dogs compared with control animals. The sorbitol concentration in erythrocytes was also significantly higher in diabetic dogs and, in particular, in poorly compensated animals and in dogs with ketonuria. In five cases that were analysed before and after clinical improvement, sorbitol concentration was found to correlate with improvement. These results suggest that non-specific glycosylation is increased and that the polyol pathway is activated in diabetic dogs in a manner that is proportionate to the severity of disease. Moreover, the concentration of AGE products and sorbitol may be useful for monitoring the onset of diabetic complications and assessing the most appropriate therapeutic approaches for management of canine DM. 相似文献
3.
Early watercore was observed in apples (Malus domestica Borkh.) from the outer canopy of ‘Orin’, an early watercore susceptible cultivar, while there was no watercore in ‘Orin’ fruit from the inner canopy and resistant ‘Fuji’. Sorbitol content was higher not only in the free space but also in the vacuole and cytoplasm in watercored ‘Orin’ than in other non-watercored fruit. Comparing watercored apples to non-watercored fruit of ‘Orin’, higher fructose and glucose in the vacuole and no significant difference in the distribution rate of sorbitol to each compartment were observed. Watercored ‘Orin’ apples showed significantly higher permeability of tonoplast to fructose, glucose, and sorbitol than those in other non-watercored apples. The permeability of plasma membrane to each sugar was much higher than that of tonoplast but no marked difference was observed among the treatments. The results suggest that metabolism of sorbitol could be active even in watercored ‘Orin’ apples and the accumulated sorbitol in the intercellular spaces might be primarily due to an active unloading from the phloem and not be brought by increased leakage from the cells. 相似文献
4.
本研究以马铃薯品种(品系)‘克新一号’、‘夏坡蒂’、‘K1’和‘K2’组培苗为试验材料,以不同浓度的甘露醇和山梨醇模拟干旱胁迫,研究干旱胁迫对马铃薯组培苗生育进程及试管薯产量的影响。结果表明:在遭受干旱胁迫下,不同马铃薯品种的叶片数、株高均会随干旱胁迫而降低;不同马铃薯品种叶和茎干物质量的变化随着干旱胁迫程度的加重而出现不同程度的降低趋势;不同品种马铃薯的试管薯结薯总数与大中薯率随着干旱胁迫程度的增加出现降低趋势,低浓度(0.1 mol/L)的甘露醇和山梨醇会诱导结薯提前,推测可能是马铃薯面临干旱胁迫的一种应对机制。本研究结果可为马铃薯节水抗旱技术研究及抗旱品种筛选提供一定的理论依据。 相似文献
5.
山梨醇对籼稻愈伤组织培养的效应 总被引：8，自引：0，他引：8
以6个籼稻品种为供试材料,研究结果肯定了培养基中附加成分山梨醇在愈伤组织继代培养中,具有促进愈伤组织生长及提高其分化潜力的效果;籼稻亚种中的不同品种,愈伤组织的生长及再生植株能力,在继代培养的后期表现出明显的品种特异性;分化培养基中加入不同浓度配比激素,对诱导器官分化的效果有明显差别,激动素(KT)与萘乙酸(NAA)的比例为5:1有利于取得根、芽生长的同步性。 相似文献
6.
Greenhouse-grown 1-year-old potted M.9EMLA apple trees (Malus pumila Borkh) were subjected to the soil compaction and, after growing under compacted or non-compacted conditions for 6 weeks, were subjected to drought stress by withholding water for an additional six-week period. Soil compaction and drought stress significantly reduced plant height, number of leaves, and leaf area. Although drought significantly inhibited photosynthesis and transpiration, compaction only depressed transpiration. Furthermore, the effects of drought on plant growth, photosynthesis and transpiration were much greater than the effects of compaction. The rate of water loss from compacted plants was lower than the rate from non-compacted controls and this may explain the insignificant impact of compaction on photosynthesis. Sorbitol, glucose, and fructose concentrations increased over time during the drought stress period whereas sucrose concentration declined. In well-watered controls, sucrose concentration was much higher in leaves of compacted plants than in the leaves on non-compacted controls. For most of the sampling dates the leaf sorbitol concentration was lower in leaves on plants growing in compacted soil than in the leaves of those of the non-compacted controls. Although interactions between the effects of compaction and drought were highly significant for plant growth variables during the onset of drought, interactive effects on photosynthesis, transpiration, relative water content and carbohydrate variables were inconsistent. Compaction and drought both have major effects on apple plants and the interactions between these two stresses are complex. 相似文献
7.
8.
山梨醇对植株抗逆性作用的研究进展 总被引：2，自引：1，他引：1
山梨醇作为一种重要的植物内源物质,广泛参与调控植株防御反应和形态发育相关的多种生物学过程,应对各种环境胁迫的应激反应,因此山梨醇使植株获得抗性的研究受到广泛关注。本文综述了近年来山梨醇在植株抗逆性研究中取得的进展,包括植物在遭受环境胁迫时植株体内山梨醇含量的变化,以及外源山梨醇在促进植株对营养元素的吸收和提高愈伤组织诱导率等方面的应用研究,提出了当前山梨醇研究中存在的问题,并对山梨醇提高植株抗逆性在我国高产优质高效农业发展中所起的作用进行了展望。 相似文献
9.
为研究6-磷酸山梨醇脱氢酶基因在乳酸菌中超表达的影响,本试验根据副干酪乳杆菌的基因序列设计引物,用PCR的方法扩增6-磷酸山梨醇脱氢酶(gutF)基因,将其连接到乳酸菌表达载体pMG36e,并将重组质粒转化到副干酪乳杆菌及乳酸乳球菌中获得重组菌株;对重组菌株表达产物进行蛋白质水平的电泳检测,检测出表达了大约28kD的蛋白;使用山梨醇替代培养基中的葡萄糖,以未转化基因菌株做阴性对照,进行生长曲线的测定,结果表明,转化菌株能够利用在以山梨醇作为碳源的条件下,重组菌株的生长速度和最终菌浓度均优越于对照菌株。本研究通过超表达6-磷酸山梨醇脱氢酶蛋白,对于进一步研究乳酸菌的耐受性具有十分重要的理论基础。 相似文献
10.
In composite (scion–rootstock) dwarfing fruit trees, an overgrowth at the graft union is often observed, the severity of which is correlated with degree of dwarfing. The graft union of dwarfing sweet cherry (Prunus avium L.) rootstocks may limit soluble sugar transport or starch mobilization, leading to localized accumulation. Soluble sugars and starch were measured in the tissues surrounding the graft union of young ‘Rainier’ (2002) and ‘Lapins’ (2003) sweet cherry trees on ‘Gisela 5’ (‘Gi 5’; dwarfing) and ‘Colt’ (vigorous) rootstocks. Separate rootstock shank, rootstock, graft union, and scion tissues were analyzed for both starch and soluble sugar content throughout the growing season in both years. Starch concentrations did not vary among locations within the graft union for ‘Rainier’ on either the dwarfing or vigorous rootstock, or for ‘Lapins’ on the dwarfing stock. However, for ‘Lapins’ on ‘Colt’, starch was highest in the rootstock shank and declined vertically (rootstock shank ? rootstock > union > scion). Soluble sugar concentrations were generally similar to or higher in scions on ‘Gi 5’ than on ‘Colt’, and were similar to or lower in the rootstock and rootstock shank on ‘Gi 5’ than on ‘Colt’. Results suggest that rootstock has a significant effect of localized accumulations of carbohydrates above and within the graft union of ‘Gi 5’ and below the graft union of ‘Colt’. 相似文献