排序方式： 共有12条查询结果，搜索用时 31 毫秒
1.
Legumes contain a range of non‐nutritional phytochemicals that may have health‐promoting effects in humans. In this study, we determined the concentrations of four phytoestrogens (coumestrol, apigenin, luteolin and quercetin) in field‐grown alfalfa (Medicago sativa L.). Differences between plants of different stages of maturity, between plant parts, and different canopy segments were assessed. The concentration of individual phytoestrogen in whole herbage varied between 15 and 225 μg g?1 dry matter (DM) and was strongly affected by stage of maturity. Coumestrol and apigenin concentrations were highest at early vegetative stages, luteolin and quercetin at early vegetative and late flowering stages. All phytoestrogens were found in lowest concentrations at the early flowering stage (average 68 μg g?1 DM); stage at which alfalfa is usually harvested when used as a forage source for animals. At vegetative stages, apigenin was the predominant phytoestrogen in herbage followed by coumestrol, the reverse being observed upon initiation of flowering; luteolin and quercetin were found at all stages in similarly lower concentrations. Concentrations of luteolin, quercetin and apigenin were 225, 410 and 690 % greater, respectively, in flowers than in leaves or stems; coumestrol concentration was similar between plant parts. In flowers and stems the predominant phytoestrogens were apigenin and quercetin, followed by coumestrol and luteolin. Similar concentrations (average 26 μg g?1 DM) of each of the four phytoestrogens were found in leaves. Concentrations through the herbage canopy varied and were greatest at >60 cm from the soil surface for apigenin and coumestrol, but greatest at >60 and 0–20 cm for quercetin and at 0–20 cm for luteolin. The results suggest that if alfalfa is to be used as a source of phytoestrogens and is harvested for the production of herbal supplements or nutraceuticals, management will need to be adapted. 相似文献
2.
Mohammed F. Aldawsari Mohammed Muqtader Ahmed Farhat Fatima Md. Khalid Anwer Prakash Katakam Abdullah Khan 《Marine drugs》2021,19(8)
The objective of this work was to develop sustained-release Ca-alginate beads of apigenin using sodium alginate, a natural polysaccharide. Six batches were prepared by applying the ionotropic gelation technique, wherein calcium chloride was used as a crosslinking agent. The beads were evaluated for particle size, drug loading, percentage yield, and in vitro drug release. Particle size was found to decrease, and drug entrapment efficiency was enhanced with an increase in the polymer concentration. The dissolution study showed sustained drug release from the apigenin-loaded alginate beads with an increase in the polymer proportion. Based on the dissolution profiles, BD6 formulation was optimized and characterized for FTIR, DSC, XRD, and SEM, results of which indicated successful development of apigenin-loaded Ca alginate beads. MTT assay demonstrated a potential anticancer effect against the breast cancer MCF-7 cell lines. The antimicrobial activity exhibited effective inhibition in the bacterial and fungal growth rate. The DPPH measurement revealed that the formulation had substantial antioxidant activity, with EC50 value slightly lowered compared to pure apigenin. A stability study demonstrated that the BD6 was stable with similar (f2) drug release profiles in harsh condition. In conclusion, alginate-based beads could be used for sustaining the drug release of poorly water-soluble apigenin while also improving in vitro antitumor, antimicrobial, and antioxidant activity. 相似文献
3.
Yifei Pei Li Yang Lin Wu Huanshan He Guoxia Geng Dejun Xu Huali Chen Qingwang Li 《Animal Science Journal》2018,89(7):956-965
The main aim of the present study was to evaluate the cryoprotective effect of apigenin (AP) and ferulic acid (FA) on boar sperm during cryopreservation. AP and FA were both demonstrated to be high‐efficiency antioxidants and had not previously been used to protect sperm from cryodamage. As boar sperm is sensitive to oxidative stress, suitable antioxidants are still needed for improving frozen‐thawed sperm quality. With this purpose, semen samples coming from five boars were used in this study. Ejaculates of five boars were mixed and split into 16 aliquots, in which different doses of AP and FA were added separately or together. The motility, the plasma membrane integrity, the mitochondrial activity, the acrosomal integrity, the antioxidase activities and the malondialdehyde concentration of the frozen‐thawed boar sperm were assessed. The results suggested that both AP and FA significantly improved the frozen‐thawed boar sperm quality in all these aspects when they were added to the freezing extender separately, while the highest improvement was recorded when the extender was supplemented with 0.1 mmol/L AP plus 0.15 mmol/L FA. These findings demonstrated that supplementation of freezing extender with both AP and FA had a combined, beneficial effect on frozen‐thawed boar sperm. 相似文献
4.
Alexander Sirotkin Zuzana Záhoranska Adam Tarko Zuzana Fabova Saleh Alwasel Abdel Halim Harrath 《Journal of animal physiology and animal nutrition》2021,105(1):80-89
The influence of toluene alone and in combination with plant polyphenols apigenin, daidzein or rutin on viability, proliferation (proliferating cell nuclear antigen accumulation), apoptosis (Bax accumulation) and release of progesterone (P), testosterone (T) and estradiol (E) in cultured porcine ovarian granulosa cells was evaluated. Toluene reduced ovarian cell viability, proliferation and E release; it promoted P release, demonstrating no effect on apoptosis or T output. Apigenin alone failed to affect cell viability, proliferation, apoptosis and P and T release, but stimulated E release, promoting the inhibitory action of toluene on proliferation, preventing and even reversing the stimulatory effect of toluene on apoptosis and P. Daidzein alone reduced cell viability and promoted T release, preventing and reversing the stimulatory effect of toluene on cell proliferation. Rutin administration reduced cell viability and E output, promoting the inhibitory action of toluene on cell viability and stimulatory effect on P release, and preventing the inhibitory action of toluene on E release. Toluene reduced apigenin- and rutin-induced E release, promoting action of daidzein on cell viability. These observations suggest the action of toluene and plant polyphenols on ovarian cell functions and the functional interrelationships between these molecules in the ovary. 相似文献
5.
A. M. Rizk F. M. Hammouda S. I. Ismail N. M. Hassan M. M. El-Missiry F. A. Ahmad 《Plant foods for human nutrition (Dordrecht, Netherlands)》1990,40(1):1-3
The study of the flavonoids of the unripe fruits ofGlossonema edule, resulted in the isolation and identification of apigenin 6,8-di-C-glucoside (violanthin) and luteolin 7-O-glucoside. 相似文献
6.
7.
Santosh Shiwakoti Dennis A. Shannon C. Wesley Wood Nirmal Joshee Agnes Rimando Kathy S. Lawrence 《Journal of plant nutrition》2016,39(9):1240-1249
Greenhouse experiments were conducted to determine the effects of nitrogen (N), phosphorus (P), and potassium (K) fertilizer on biomass yield and flavonoid content of American skullcap (Scutellaria lateriflora). Each experiment was carried out two times and consisted of six levels of each nutrient. The regressions gave maxima for dry matter, baicalein yield, and chrysin yield at 446, 412, and 351 kg N/ha for N fertilizer, respectively. Dry matter yield exhibited linear response to P application. The yield of scutellarein, baicalin, baicalein, and chrysin increased with addition of P. The regression gave maximum for dry matter at 208 kg K/ha for K fertilizer. A linear response to K fertilization was observed for scutellarein concentration. P application had the greatest effect on the flavonoids analyzed, whereas K had least, which may be attributed in part to the presence of K in the fritted clay medium. 相似文献
8.
高等植物中花青素合成与芹菜素合成之间存在共同的前体物质。利用非紫色芹菜‘六合黄心芹’和紫色芹菜‘南选六合紫芹’(从‘六合黄心芹’中选择而来)的叶柄为花青素和芹菜素代谢研究材料,利用荧光定量PCR方法检测花青素和芹菜素合成相关基因的表达水平。研究结果表明,‘六合黄心芹’叶柄中未检测到花青素积累,而‘南选六合紫芹’叶柄中的花青素含量呈现较高水平(0.0523 mg·g~(-1)FW)。‘六合黄心芹’叶柄芹菜素含量(0.0172 mg·g~(-1) FW)显著高于‘南选六合紫芹’(0.0124 mg·g~(-1) FW)。荧光定量PCR结果表明,除了AgFNS之外,其余花青素和芹菜素代谢相关基因(AgPAL、AgC4H、AgCHS、AgCHI、AgFNS、AgF3H、AgF3’H、AgDFR、AgANS和Ag3GT)在‘南选六合紫芹’叶柄中的表达量显著或者极显著高于‘六合黄心芹’;黄酮合成酶基因AgFNS在‘六合黄心芹’叶柄中的表达量为‘南选六合紫芹’的11.69倍。 相似文献
9.
The present study was aimed to investigate the anti-arthritic activity of alcoholic extract ofClerodendron inerme which was shown to inhibit inflammatory process. Arthritis was induced in rats by injecting rats intradermally with Freund＇s adjuvant 0.01%-0.1% in paraffin oil. They were grouped and received 10 mg/100 gm b.wt. of alcoholic fraction of C. inerme, 1.5 mg/100 gm b.wt. hydrocortisone and saline, orally, for 15 days. The arthritic scores, radiological scores, body weights, liver glycogen, serum mucoproteins were monitored for 49 days. Wilcoxan ranking methods and Student＇s t Test were used to determine the significance changes between control and treated groups. Adjuvant-arthritic rats showed a significant decrease in body weight, liver glycogen, increased serum mucoprotein and severity of periostitis and other degenerative changes m the bone. But treatment with the alcoholic fraction （apigenin, scutallarin and pectinolinergenin） of C. inerme exhibited a very good relief from the arthritic conditions by increasing the body weight, increased liver glycogen content and registered little degenerative changes in bones, suggesting long term anti-arthritic activity when compared to the control group. The anti-arthritic activity ofC. inetme alcoholic fraction was remarkable in this study. However, further studies are required to elucidate its full anti-arthritic potential. 相似文献
10.
楸树叶中抗氧化活性成分的分离和鉴定 总被引：1，自引：0，他引：1
前期研究发现,楸树叶粗提物的乙酸乙酯萃取相具有较强的抗氧化活性。为了进一步明确其抗氧化活性成分,以硅胶柱色谱结合清除 DPPH自由基法,寻找抗氧化能力较强的组分,并利用半制备液相色谱对该组分进行分离、纯化,以清除 DPPH自由基、还原力和清除 ABTS自由基能力作为考察指标,根据理化性质及光谱数据鉴定化合物。结果从楸树叶粗提物的乙酸乙酯萃取相中分离得到了2个化合物,分别为木犀草素(1)和芹菜素(2)。化合物1的抗氧化活性强于BHT和化合物2;化合物1清除DPPH自由基、还原力及清除ABTS自由基的IC50值分别为23.89,29.77和43.47μg mL -1;化合物1的抗氧化活性强于化合物2,主要因其在黄酮类化合物母核的 B环上具有邻二酚羟基结构。木犀草素和芹菜素2个化合物首次从楸树叶中分离得到。 相似文献