马尾松叶表微生物多样性     

袁贵云  孙学广  郑炀  郭其强  丁贵杰 《森林与环境学报》2021,(3):318-324

为发掘对林木生长发育有利的优良微生物资源,并筛选适合叶表微生物的收集方法,以马尾松针叶为试验材料,分别用悬摇法和超声波法收集马尾松叶表微生物,用扩增子高通量测序技术、MUSCLE和Qiime软件研究马尾松叶表微生物的多样性。结果表明:扫描电镜观测结果显示,马尾松针叶表面定殖有大量微生物,包括真菌(菌丝及孢子)和细菌。扩增子高通量测序结果表明,马尾松叶表微生物物种丰富,包含细菌运算分类单位(OTUs)490个,真菌OTUs 1273个。马尾松叶表细菌以未分类的蓝细菌属(unidentified_Cyanobacteria)(36.53%)、未分类的拜叶林克氏菌属(unidentified_Beijerinckia)(28.60%)、鞘氨醇单胞菌属(Sphingomonas)(2.35%)为优势属;叶表真菌以枝孢属(Cladosporium)(2.45%)、拟盘多毛孢属(Pestalotiopsis)(0.92%)、无头孢菌属(Capnobotryella)(0.91%)为优势属。针对叶表细菌多样性的研究表明,悬摇法和超声波法均有较高的物种检出度;在叶表真菌多样性的研究中超声波法优于悬摇法,但超声波法样品间数据变异性较大,测定结果不稳定。  相似文献   

5种生物制剂对马铃薯种薯萌芽、极端天气下植株生长和产量性状的影响   总被引：1，自引：1，他引：0  

淳俊  桑有顺  冯焱  陈涛  李倩 《中国农学通报》2021,37(29):1-6

旨在满足马铃薯生产中茬口衔接、机械化生产技术应用、不利气候下稳产等对马铃薯出苗早、齐、壮的需求,以‘费乌瑞它’为供试品种,用基于有益活菌或工程菌提取物的5种生物制剂进行种薯处理,对多重性状进行了对比分析。5种生物制剂较常规化学制剂,均能够不同程度地促进种薯萌芽和芽根同生,出苗期提前2~7天,播种后49天的出苗率提高3.33%~17.78%。其中,表现最好的为酵母核苷酸衍生物和VDAL,种薯萌发和生根均显著高于对照。霜冻后,生物剂拌种处理在恢复前期促进植株生长,由此促进恢复后期的块茎发育,较常规化学处理增产8.39%~24.03%,体现了不同程度的保产效果。多马道黑、酵母核苷酸衍生物、根肽和VDAL体现出较好的保产效果,可作为种薯处理剂投入马铃薯生产。  相似文献   

Beaver dams,streamflow complexity,and the distribution of a rare minnow,Lepidomeda copei          下载免费PDF全文

Daniel C. Dauwalter  John D. Walrath 《Ecology of Freshwater Fish》2018,27(2):606-616

Freshwater fishes are threatened globally, and often too little is known about threatened species to effectively guide their conservation. Habitat complexity is linked to fish species diversity and persistence, and degraded streams often lack habitat complexity. Beaver Castor spp., in turn, have been used to restore streams and increase habitat complexity. The northern leatherside chub Lepidomeda copei is a rare, small‐bodied, drift‐feeding minnow that has anecdotally been observed to use complex habitats associated with beaver dams in the western United States. To investigate this anecdote, we conducted fish and habitat surveys, the latter focusing on quantifying habitat complexity, in a sub‐basin of the Upper Snake River Basin in the USA. Complementary generalised linear model and path analyses revealed that northern leatherside chub occurred more often at sites with complex streamflows, and streamflows were more complex when beaver dams were present and pools were deeper. Northern leatherside chubs were also more likely to occur when temperatures were warmer, aquatic macrophytes were abundant and stream channels were narrow and deep. The linkage between chubs, complex streamflows and beaver dams needs to be evaluated more broadly to completely understand its role in the rangewide status of the species. However, it does suggests that increased use of beaver reintroductions and dam analogues for stream restoration could be a boon for the northern leatherside chub, but such efforts should be monitored to determine their effectiveness to help adapt beaver‐based restoration approaches to best benefit the species.  相似文献   

基于P-S-R框架模型试点研究高寒地区生态承载力——以甘肃省迭部县为例     

李真  李振林  屈鹏 《中国农学通报》2020,36(19):85-92

全面客观评价生态资源承载能力,进而为区域可持续发展提供理论依据。以甘肃省迭部县为例,首先,采用压力-状态-响应(P-S-R)模型构建生态承载力评估指标体系,运用层次分析法(Analytic Hierarchy Process,AHP)确定各指标权重,评估生态环境承载力现状;其次,在生态功能区层面对迭部县水源涵养功能及生物多样性功能进行评价;最后,对生态承载力状况进行成因解析。研究结果表明：（1）迭部县整体生态承载力得分为0.239,处于“较强”承载力状态,构成整体生态承载力的生态弹性力、资源和环境承载力和人类社会影响力得分分别为0.222、0.062和0.063。（2）2016年,迭部县单位面积水源涵养量为111.76 mm,水源涵养功能处于“高”状态,其中,水源涵养功能较高地区主要集中在以草地和针阔混交林为主的区域。（3）自然栖息地面积占迭部县总面积的83.67%,其自然栖息质量指数(Natural Habitant Quality Index,NHQI)处于“高”状态。（4）从自然和经济2个因素进行分析,发现迭部县生态环境条件较好,但林草地面积已呈现出逐渐减少状态,因此需要通过合理科学的开发引导,发展文化旅游事业,推动迭部县的可持续发展。  相似文献   

The microbial diversity and structure in peatland forest in Indonesia     

Bing Liu  Muhamed Jamal Hosen Talukder  Eeva Terhonen  Maija Lampela  Harry Vasander  Hui Sun  Fred Asiegbu 《Soil Use and Management》2020,36(1):123-138

The microbial community structure and function under forest in tropical peatlands are poorly understood. In this study, we investigated the microbial community structure and diversity in natural peat swamp forest soil, disturbed peat soil and mineral soil in Central Kalimantan, Indonesia, using 454 pyrosequencing. The results showed that the natural peat soil had the greatest fungal species richness (Chao1), which was significantly (p < .05) larger than that in the other two soils. Community structure of both fungi and bacteria in natural peat soil differed significantly from that in the disturbed peat soil (p = .039 and p = .045, respectively). Ascomycota (40.5%) was the most abundant phylum across the three soils followed by Basidiomycota (18.8%), Zygomycota (<0.1%) and Glomeromycota (<0.1%). The linear discriminant analysis with effect size (LEfSe) showed that Ascomycota (p < .05) and genus Gliocephalotrichum (p < .05) dominated in natural peat soil. Functionally, pathotrophs were more abundant in disturbed peat soil (p < .05). Proteobacteria (43.8%) were the most abundant phylum followed by Acidobacteria (32.6%), Actinobacteria (9.8%), Planctomycetes (1.7%). Methylocystis, Telmatospirillum, Syntrophobacter, Sorangium and Opitutus were the more abundant genera in disturbed peat soil, whereas Nevskia and Schlesneria were more abundant in mineral soil and natural peat soil, respectively. The natural peat forest soil supported a more diverse microbiology; however, the land use of such a soil can change its microbial community structure. The results provide evidence that the disturbance of tropical peat land could lead to the introduction and spread of a large number of fungal diseases  相似文献   

捕食线虫性真菌的分离培养及分布规律   总被引：6，自引：1，他引：5  

杨晓野  杨莲茹  刘珍莲  董世娟  考桂兰 《中国兽医学报》2003,23(4):344-347

对自然界中的捕食线虫性真菌进行了分离和种属区分，并对分布规律进行了研究。从土壤、粪便等材料中获得了2类活性较强的捕食线虫性菌株：即套捕型(hooping)捕食线虫性真菌和粘捕型(sticking)捕食线虫性真菌。主要的代表种类有3种。这些捕食线虫性真菌在捕食性结构分生孢子形态及某些生物学特性上有一定差异。套捕型捕食线虫性真菌代表种为少孢节丛孢菌(Arthrobotrys oligospara)和梨形指环菌(Dactylaria pyriformis)，它们以菌环、菌网作为捕食性结构(器官)，以套捕方式杀灭线虫幼虫。梨形指环菌可产生多量厚垣孢子(chlamyalospore)。粘捕型捕食线虫性真菌代表种为纺锤隔指孢菌(Dactylella ellipsospora)。可形成菌结捕食线虫性结构，以粘捕的方式杀灭线虫幼虫。结果表明：捕食线虫性真菌在土壤和家畜粪便中的分离率是40．41％；此类菌适合于生存在阴暗、潮湿、富含腐植质的环境中。在温暖季节时，采用0．4g／L玉米粉琼脂培养基(CMA)易于对其进行分离培养。  相似文献   

捕食线虫性真菌菌种保存技术的研究     

考桂兰  石鑫  杨晓野  杨莲茹  刘珍莲  王军 《中国预防兽医学报》2004,26(5):385-388

本试验对捕食线虫性真菌菌种保存技术进行了研究,测定了在不同介质保存条件下,捕食线虫真菌分生孢子和菌丝的存活情况及其不是寄生性线虫的能力.结果表明:在设定的保存条件下,分别经过1周冷冻融化或2周反复冷冻融化,被保存的分生孢子接种于玉米粉琼脂培养基上能生长发育:在加入线虫第3期幼虫后,能生产捕食器,对虫体发挥捕食作用.  相似文献   

Leptin对动物繁殖机能的影响及其应用前景   总被引：2，自引：0，他引：2  

张海艳  岳文斌 《畜牧兽医杂志》2003,22(3):14-16

Leptin是由脂肪组织分泌的一种“厌食”激素。自1995年发现以来，就成为最活跃的研究领域。它可通过下丘脑或直接作用于卵巢，对动物青春期的发育、生殖器官的发育以及性激素的分泌都有重要的调节作用。本文综述了Leptin的生物学特性、作用机理及其在繁殖方面的作用，井指出了它在畜牧生产中应用前景。  相似文献   

Genetic diversity of herbicide-resistant and -susceptible Avena fatua populations in North Dakota and Minnesota     

L W MENGISTU  C G MESSERSMITH  & M J CHRISTOFFERS 《Weed Research》2005,45(6):413-423

Genetic diversity within and among 20 herbicide-resistant (HR) and 16 herbicide-susceptible (HS) Avena fatua multi-field populations was determined using 82 polymorphic loci resulting from two intersimple sequence repeat (ISSR) primers and one long-primer random amplified polymorphic DNA (LP-RAPD) primer. Collections from the Red River Valley of North Dakota and Minnesota, sampled in 1964 and 2000, represented A. fatua populations before and after intensive exposure to herbicides. A 1995 collection from south-west North Dakota represented A. fatua exposed to low herbicide selection. Despite differences in years of herbicide exposure among collections, both HR and HS populations from every collection maintained nearly similar levels of ISSR and RAPD diversity. Genetic differentiation among populations (G_ST) varied from 11% to 13% among HR populations and from 9% to 16% among HS populations, indicating that 84–91% of total variation remained within HS or within HR populations. Minimal difference in gene diversity between HR and HS is consistent with multiple origins of resistance, where HR A. fatua most likely evolved from diverse founding individuals.  相似文献   

江西三种危害食用菌的绿色霉菌生物学特性研究     

邱贵根  涂寿兰 《江西农业学报》1992,4(2):143-150

本文报道了江西三种危害食用菌的绿色霉菌的生物学特性。它们是:绿色木霉(Trichoderma Viride pers exFn)。产黄青霉(Penicillium Chrysoenum Thom)和构巢曲霉(Aspergillus nidulcms(Eidzm)Winter)。  相似文献