苜蓿皂甙的理化性质及其生物学功能   总被引：7，自引：0，他引：7  

张勇  林东康  汪儆 《中国饲料》2006,(4):37-38

虽然苜蓿皂甙作为一种抗营养因子限制了苜蓿在畜牧生产中的广泛应用,但苜蓿皂甙能够降低单胃动物血浆胆固醇,为人类提供安全、保健的畜产品。为此本文对苜蓿皂甙的理化性质、制备及生物学功能进行了综述。  相似文献   

红芪愈伤组织中黄酮代谢途径的探讨   总被引：3，自引：1，他引：2  

张缨  邢更妹 《甘肃农业大学学报》2000,35(1):63-65,69

在红氏供试材料中苯丙氨酸转氨酶活性在分化愈伤组织中最高，但游离苯氨丙酸含量在分化愈伤组织中并无明显降低，说明游离氨基酸的积累主要来自蛋白质的分解。黄酮含量的变化以褐化愈伤组织中含量最高，因此我们推测：黄酮合成的最后几步，可能发生在褐化愈伤组织中，从可溶性蛋白质的ＳＤＳ－ＰＡＧＥ平板电泳结果表明，分化愈伤组织与褐化愈伤组织相比蛋白质谱带发生了明显的变化，这可能与控制黄酮合成的有关酶的活性变化有关。  相似文献   

固相萃取——高效液相色谱法分离红花中的黄酮类物质     

张朋美  赵娜  韩黎黎 《张家口农专学报》2008,(4):20-21

目的 研究红花中的黄酮类物质。方法 乙醇浸泡提取，固相萃取分离，液相色谱法进行纯度检测，结果 采用的红花中有两种黄酮类物质。结论 洗脱液V（H2O）：V（C4H5OH）=3：7，能很好的分离红花中的两种黄酮类物质。  相似文献   

拉缘蝽为害对卵叶小蜡内含物含量的影响     

张潮巨 《福建林业科技》2006,33(3):60-64

通过对受拉缘蝽不同程度为害的卵叶小蜡叶片的单宁、黄酮、总酚含量的测定,结果表明:受害的卵叶小蜡叶片的单宁含量随着拉缘蝽若、成虫为害程度的加重而发生变化,在受害初期显著增加,但随着为害程度的加重,而呈逐渐下降的趋势;受害卵叶小蜡叶片中黄酮含量随着受害程度的加剧,呈先上升、而后再下降的特点;叶部总酚的含量随着受害程度的不断加重,总酚的含量呈现上升的趋势。说明卵叶小蜡应激反应的过程是一种随着受害程度的不同而不断变化的动态过程。  相似文献   

紫苏子皮提取物中抗氧化性成分及作用研究   总被引：7，自引：2，他引：7  

王天元 《林产化学与工业》2002,22(1):63-67

对紫苏子皮的成分及性质进行研究，用实验方法证明该皮含有丰富的黄酮及多酚类化合物，具有防止油脂氧化的功能。可用于代替化学合成抗氧化剂。不仅无毒，而且不具有保健作用。  相似文献   

Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells     

Juanjuan Wang  Hua Han  Xiangfeng Chen  Yanghua Yi  Hongxiang Sun 《Marine drugs》2014,12(8):4274-4290

The cytotoxic effects of thirteen triterpene glycosides from Holothuria scabra Jaeger and Cucumaria frondosa Gunnerus (Holothuroidea) against four human cell lines were detected and their cytotoxicity-structure relationships were established. The apoptosis-inducing activity of a more potent glycoside echinoside A (1) in HepG2 cells was further investigated by determining its effect on the morphology, mitochondrial transmembrane potential (Δψ_m) and mRNA expression levels of the apoptosis-related genes. The results showed that the number of glycosyl residues in sugar chains and the side chain of aglycone could affect their cytotoxicity towards tumor cells and selective cytotoxicity. 1 significantly inhibited cell viability and induced apoptosis in HepG2 cells. 1 also markedly decreased the Δψ_m and Bcl-2/Bax mRNA express ratio, and up-regulated the mRNA expression levels of Caspase-3, Caspase-8 and Caspase-9 in HepG2 cells. Therefore, 1 induced apoptosis in HepG2 cells through both intrinsic and extrinsic pathway. These findings could potentially promote the usage of these glycosides as leading compounds for developing new antitumor drugs.  相似文献   

Inheritance of flower colour and flavonoid pigments in Gerbera     

A. Tyrach  W. Horn 《Plant Breeding》1997,116(4):377-381

The flower colour of Gerbera, an important ornamental cut flower, is derived from carotenoids and flavonoids. The knowledge of enzymological and genetic control of flavonoid biosynthesis is still incomplete. The present paper summarizes the results obtained at our institute between 1981 and 1993. The material for the investigation of phenotypic segregation and segregation of flavonoids after chromatographic analysis came from 408 progenies of controlled crosses. Phenotypic segregation analysis showed acyanic genotypes to be homozygous recessive and recessive epistatic over cyanic genotypes, respectively. This was confirmed by the existence of two loci controlling steps in biosynthesis (fht, dfr or ans) showing recessive mutants and complementary gene action after crosses. Flavone formation is effected by one dominant allele (fns⁺); dominant and recessive genotypes are now available. Regarding anthocyanidin inheritance, an unusual epistasis of 4′-hydroxylation (pelargonidin formation) over 3′,4′-hydroxylation (cyanidin formation) was observed. Final proof of the postulated gene actions will come from enzymological and molecular biological investigations of the chemogenetically defined Gerbera genotypes now available.  相似文献   

Two new chalcone glycosides from the stems of Entada phaseoloides     

Zhao ZX  Jin J  Lin CZ  Zhu CC  Liu YM  Lin AH  Liu YX  Zhang L  Luo HF 《Fitoterapia》2011,82(7):1102-1105

Two new chalcone glycosides 4′-O-(6″-O-galloyl-β-d-glucopyranosyl)-2′,4-dihydroxychalcone (1) and 4′-O-(6″-O-galloyl-β-d-glucopyranosyl)-2′-hydroxy-4-methoxychalcone (2) together with one known chalcone glycoside 4′-O-β-d-glucopyranosyl-2′-hydroxy-4-methoxychalcone (3) were isolated from the stems of Entada phaseoloides. The structures of the new compounds were elucidated on the basis of extensive spectroscopic analysis, including HSQC, HMBC, ¹H–¹H COSY, and chemical evidences. This is the first report of chalcone-type compounds isolated from the genus Entada.  相似文献   

蝙蝠葛茎叶抗菌活性成分的研究   总被引：5，自引：0，他引：5  

孔阳  马养民  余博 《西北农林科技大学学报(自然科学版)》2005,33(4):151-153

将蝙蝠葛(Menispermum.dahuricumDC.)茎叶的乙醇粗提物分别用石油醚、乙酸乙酯、正丁醇萃取。其萃取物抗菌活性试验结果表明,石油醚萃取物对青霉菌(Penicilliumsp.)有强抑制作用;乙酸乙酯萃取物对假丝酵母菌(Candiasp)有强抑制作用,对金色葡萄球菌(Staphylococcusaureus)和大肠杆菌(Escherichiacoli)有较强抑制作用,对辣椒疫霉菌(Phytophoracapsic)有抑制作用;正丁醇萃取物无明显抗菌活性。经对乙酸乙酯萃取物分离纯化得到2个化合物和,抗菌试验结果表明,化合物具有抑制金色葡萄球菌和辣椒疫霉菌的活性,化合物具有抑制假丝酵母菌和辣椒疫霉菌的活性;经光谱和化学分析鉴定化合物为异槲皮素甙,该化合物为首次从蝙蝠葛植物中得到。  相似文献   

超声波辅助提取荆芥叶中总黄酮的方法研究   总被引：4，自引：0，他引：4  

游新侠  仇农学 《郑州牧业工程高等专科学校学报》2006,26(1):13-15

本试验运用超声波辅助技术提取荆芥叶中的总黄酮,用亚硝酸钠-硝酸铝比色法测定总黄酮的含量。通过单因素试验、正交试验、对照试验,确定荆芥中总黄酮提取的最佳方案:超声功率700 W,荆芥质量2.00 g,温度45℃;物料比1∶60,乙醇浓度70%,提取时间为15 min。最后得荆芥中总黄酮的最大提取率为1.592%。  相似文献