The Prospects of Tissue Culture and Genetic Engineering for Strawberry Improvement     

S. Mohan Jain  Eija Pehu 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):133-139

Abstract

Strawberry shoot meristem cultures have been used for producing virus-free strawberry plants. Plantlet regneration from leaf mesophyll protoplasts, and Agrobacterium tumefaciens mediated genetic transformation, mark the beginning of strawberry improvement with biotechnologies. The use of biotechnological tools has a greater potential in producing strawberry plants with traits such as early maturation, frost and disease resistance, mite and nematode resistance, high yield, better quality, suitability for mechanical harvesting, shipping ability, ellgaic acid content, aroma compounds and cost-effective micropropagation. Gene identification and mapping with RFLPs or RAPDs would be desirable. Transgenic strawberries should be tested for their quality before releasing to the consumers.  相似文献   

植物光自养微繁技术研究进展   总被引：6，自引：0，他引：6  

管道平  杨其长  刘文科  肖平  杨建荣 《园艺学报》2006,33(3):680-686

 光自养微繁技术是一种全新的植物组织培养技术, 从提出该理论至今, 许多研究者已在光自养微繁方面做了大量的尝试, 使这一新技术逐步完善。本文对光自养微繁的研究进展进行了综述, 介绍了光自养微繁研究现状、研究热点、在种苗工厂化生产中的应用, 并提出光自养微繁技术研究动向。  相似文献   

植物无糖组培快繁装置及其环境控制系统的研制     

杨其长  刘文科  管道平 《中国农业科技导报》2007,9(4):79-84

针对常规植物组培存在的问题,从植物无糖组培实用化角度出发,研制了带有新型CO2施放装置的180 L植物无糖组培容器及其环境控制系统,采用小流量控制、三通阀调节和PWM控制方式,实现了对CO2浓度的精确控制,控制精度达到±50 μmol·mol^-1;采用穴盘覆膜与气体循环吸附相结合的方式实现了对容器内相对湿度的自动控制,控制精度达到±22%.通过圆叶海棠的无糖组培的试验,结果表明,该系统对组培苗的生长环境和生理品质的提高具有显著的促进作用.  相似文献   

优良观赏保健树大叶冬青扩繁利用研究     

李晓储  蒋继宏  黄利斌  马东跃  黄钟玉  刘根林  李玉巧  梁珍海  蒋泽平 《江苏林业科技》2004,31(5):1-7

在引进大叶冬青树种种质资源的基础上,系统地研究了大叶冬青生物学特性、生态适应性、生长习性、实生种播苗关键技术和组织培养及其微繁技术,以及生态复合拟生栽培技术,培育出了大叶冬青绿化苗木18万株。并测定了大叶冬青叶内可溶性蛋白质、超氧化物歧化酶(SOD)等抗衰老成分含量和叶组织中抗菌成分含量及其抑菌功能。因地制宜研制出优质嫩芽型、嫩叶炒青型、老叶微波干燥型3种苦丁保健绿茶,为大叶冬青叶资源产业化开发奠定了基础。  相似文献   

Micropropagation and callus culture of <Emphasis Type="Italic">Saussurea laniceps</Emphasis>, an alpine medicinal plant     

Chen?Yu-zhen  Li?Feng-lan?Email author 《中国林学(英文版)》2005,7(1):16-19

Cottonhead windhairdaisy (Saussurea laniceps Hand.-Mazz.) is one of the most famous and important medicinal herbs in China. Illegal collection from wild populations is increasingly threatening the present environment of S. laniceps. Establishment of an efficient method for micropropagation is the best way to change its endangered situation. When mature seeds of S. laniceps were cultured on hormone-free MS medium, plantlets were formed from germinated seeds in 7–10 d. Then 0.5 cm × 0.5 cm leaf explants were transplanted to MS medium supplemented with 1-naphthalene-acetic acid (NAA)/2,4-D and benzyladenine (BA)/KT and callus was achieved 10 d after transfer. Shoot bud regeneration occurred from callus cultured on MS medium supplemented with different growth regulators 20 d after culturing. The regeneration percentages varied with the different components of plant growth regulators. The percent regeneration from callus pretreated at low temperature of 5°C increased significantly compared with those incubated at 23/20°C directly. Optimal regeneration was observed with explants on media supplemented with 1.5 mg&#8226;L^–1 BA plus 0.2 mg&#8226;L^-1 NAA. In the presence of 0.2 mg&#8226;L^–1 NAA in half-strength MS, 78% of the shoots formed roots. Plantlets from explants showed 63% survival after acclimatization.  相似文献   

Black locust (Robinia pseudoacada L.) clonal seed orchards in Hungary     

Károly Redei  Zoltán Osváth-Bujtás  Irina Veperdi 《中国林学(英文版)》2006,(4)

Black locust (Robinia pseudoacacia L.) is one of the most important stand-forming tree species in Hungary and its importance is increasing in many countries. The main aim of the discussed new selection programme is to identify black locust clones with good performance and good form for setting up clonal seed orchards. As a result of selection programme 16 new black locust clones have been improved. In spring 2002 a black locust seed orchard was established with the newly selected clones. About 40% of the plants can be considered to belong to the height growth rate class 1 and 2. Hungary was the first country where micropropagated black locust planting material was used for seed orchard establishment.  相似文献   

马铃薯组培苗液体静置培养微繁技术研究   总被引：2，自引：0，他引：2  

牛爱国  侯丽娟  包永佶  朱海波  刘刚 《中国马铃薯》1999,(2)

在固体组织培养研究的基础上，改进培养基组成成分进行液体静置培养。本文从水、糖、琼脂对组培苗生长、增殖及培养成本三个方面进行研究。作者认为马铃薯组培苗块繁阶段培养基可以简化为液培。此法同时省去琼脂，以自来水代替蒸馏水，以食用白绵糖代替蔗糖，规模化应用效果良好，培养基成本降低了７２．９％。组培苗生长健壮，月增殖系数６．３～６．７，结薯正常。  相似文献   

Random amplified polymorphic DNA (RAPDs) markers for genetic analysis in micropropagated plants of Robinia pseudoacacia L.     

K. Kanwar  K. Bindiya 《Euphytica》2003,132(1):41-47

Four years' old micropropagated plants regenerated by enhanced axillary branching from shoot buds of a single genotype of Robinia pseudoacacia were characterized by RAPDs. Random amplified polymorphic DNA analysis was carried outusing 19 random 10-mer DNA primers and 286 RAPD bands were examined which showed 30% polymorphism. Similarity indices ranged from 0.86 to 0.96 among different plants based on RAPD data. The UPGMA dendrogram was constructed based on similarity indices which showed clustering of different plants into subgroups based on similarity values. Our results suggest that somaclonal DNA sequence variations are present even when organized cultures such as shoot buds were used as explant for micro-propagation. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

环境控制技术在植物无糖组织培养中的应用   总被引：2，自引：0，他引：2  

刘文科  杨其长 《农业工程学报》2005,21(Z2):45-49

总结了植物无糖培养的环境范畴,概述了环境控制技术在组培中的作用与研究现状,并对环境控制技术在无糖培养中的运用前景进行了讨论.  相似文献   

基于PLC的无糖组培微环境控制系统   总被引：13，自引：0，他引：13  

李传业  滕光辉  曲英华 《中国农业大学学报》2004,9(4):30-34

为实现组培微环境CO2 增施的长期自动控制 ,解决增施CO2 过程中可能将空气中杂菌引入组培箱的问题 ,设计开发了一套基于PLC的无糖组培微环境控制系统。系统以PLC为控制核心 ,采用定量供给的CO2 增施控制策略 ,可根据设定要求将固定量的高压、高纯度CO2 气体直接供入组培箱 ,并可对组培箱内湿度进行调控。系统调试试验结果表明 ,控制系统能将光期组培箱内CO2 摩尔分数和相对湿度分别控制在 70 0～ 90 0 μmol·mol-1和80 %～ 92 %的目标范围内 ,达到了预期效果  相似文献