黑胫病菌（Leptosphaeria biglobosa）在油菜叶片和茎中的侵染过程观察     

史志丹  宋培玲  郝丽芬  皇甫海燕  燕孟娇  杨永青  吴晶  赵丽丽  李子钦 《中国油料作物学报》2021,43(2):286-292

为明确黑胫病菌（Leptosphaeria biglobosa）在甘蓝型油菜叶片和茎中的侵染及扩展过程，利用绿色荧光蛋 白（GFP）标记的黑胫病菌株接菌油菜叶片，利用激光共聚焦显微镜观察菌株在油菜叶片和茎中的侵染过程。结果 表明，接种油菜叶片7 h后，分生孢子萌发并长出芽管；17 h后，芽管侵入气孔；24 h后，分生孢子全部萌发；36 h后萌 发的芽管形成菌丝；120 h后，菌丝在叶片表皮细胞间隙蔓延，并侵入叶肉细胞。13 d后，菌丝侵入茎部皮层组织； 15 d后，菌丝在皮层细胞间隙蔓延，并侵染至茎表皮；21 d后，菌丝侵染至维管组织；23 d后，菌丝侵染至茎韧皮部； 25 d后，茎导管被侵染，并向木质部扩展。本研究发现的L. biglobosa 在油菜叶片和茎中的侵染过程，可为油菜与黑 胫病菌互作的研究、黑胫病致病机理及防治提供参考。  相似文献   

Leaf carbohydrates assimilation and metabolism affect seed yield of rapeseed with different waterlogging tolerance under the interactive effects of nitrogen and waterlogging     

Jie Kuai  Xiaoyong Li  Zhen Li  Yan Xie  Bo Wang  Guangsheng Zhou 《Journal of Agronomy and Crop Science》2020,206(6):823-836

Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties.  相似文献   

Mid-storage and pre-sowing seed treatments for lettuce and carrot     

Dolly Pan  R.N. Basu 《Scientia Horticulturae》1985,25(1):11-19

Mid-storage hydration-dehydration treatments with medium-vigour lettuce seed very effectively reduced physiological deterioration in storage and ultimately showed better field emergence and final yield. Soaking-drying treatment of high-vigour lettuce seed was not effective. Pre-sowing treatment of medium-vigour lettuce seed, though less effective than mid-storage treatment, gave better field performance, but high-vigour seed did not benefit from the treatment. For carrot, both mid-storage and pre-sowing seed treatments proved beneficial, with the former showing better performance than the latter. Unlike lettuce, well-preserved carrot seed responded positively to the pre-sowing treatment.  相似文献   

猪场废水的生物处理技术及其效果   总被引：13，自引：0，他引：13  

彭里  王定勇 《家畜生态》2003,24(2):67-69,72

规模化养猪场所产生的废水污染，已成我国农村面临污染的重要来源。针对这种污染，近年来出现了多种养猪场废水处理法，其中研究和应用最广的是生物处理法。本文通过对目前国内猪场废水生物处理法的现状分析后认为：用生物处理法处理猪场废水是今后猪场废水处理的发展方向；而生物处理法自身的发展方向是混合处理法；计算机和生物工程技术将进一步与生物处理法相结合运用于猪场废水处理。  相似文献   

桑苗圃和桑园的化学除草     

黄健明  仇广泰  薛彦明 《蚕桑通报》2001,32(1):27-28

用氟乐灵和精稳杀得或高效盖草能进行桑苗圃除草，用草甘磷和乙草胺混合进行桑园除草，可有效地防除杂草。  相似文献   

Study on Site-specific Nutrient Management in Cotton Field     

YANG Li-ping  Jiang Cheng  JIN Ji-yun  ZHANG Feng-ming 《中国农业科学(英文版)》2001,(1):102-106

The study on the characteristics of spatial variability of soil nutrients and fertilizer recommendations in cotton field under certain conditions of agricultural management was conducted with GIS and systematic approach for soil nutrient constrains. The results showed that of the spatial variability of soil nutrient was greatly related to the management condition of previous crops. Grid sampling and variable rate application technology (VRAT) were the tools that would hopefully increase fertilizer efficiency. The fertilizers were applied where they were needed and at proper rate. Balance fertilization demonstration showed that fertilizer recommendations according to the available nutrient level in soil could decrease fertilizer cost with 657.4 yuan / ha and increase seed cotton yield by 19.8%. A net profit of the balanced fertilization was 5314.9 yuan / ha higher than that of local fertilization practice.  相似文献   

The Effect of Detopping the Field Bean Plants (Vicia faba L. minor) and Lack of the Pod Setting on the Symbiosis with Root Nodule Bacteria     

W. Filek    F. Dubert  M. Kaminski 《Journal of Agronomy and Crop Science》1996,176(1):39-45

The plants of field bean var. Nadwiślaνski were detopped after about 1 month since the beginning of flowering and after that all flower buds were removed from some plants. The effect of a change in the trophic balance on the setting and subsequent growth of root nodules was studied.
The removal of pods had the greater effect on the increase in the weight of the remaining organs than the detopping of plants. Detopping retarded the ageing of the plants and delayed the phase of the greatest reduction of the number of pods. The detopping of plants revealed their considerable compensative possibilities in terms of production of the pod weight and the potential of leaves for biomass production. The data obtained indicate that plant detopping may disturb the root–bacteria exchange of signals, necessary to initiate the nodule development, while the further growth of nodules depends, above all, on the amount of available photosynthates.
A very high correlation between the dry weight of the whole plant to the dry weight of leaves ratio and the weight of nodules was observed. It proves the importance of the trophic balance of the plant for the root nodule weight production.  相似文献   

乙草胺防除辣椒田杂草的药效试验   总被引：1，自引：0，他引：1  

李茹  赵桂东  周玉梅 《安徽农业科学》2001,29(5):628-628

50 %乙草胺EC 12 0 0、15 0 0ml/hm2 在辣椒苗移栽前 1d对土壤用药 ,对辣椒安全 ,除草效果在 75 .0 %～ 82 .6 %之间 ;乙草胺对禾本科杂草的防效好于阔叶草  相似文献   

河北坝上农田垄向对土壤风蚀的影响   总被引：3，自引：0，他引：3  

荣姣凤  高焕文  王晓燕 《中国农业大学学报》2004,9(3):13-15

在河北省坝上选择试验区 ,利用移动式风洞研究了农田起垄方向对土壤风蚀的影响。结果表明 ,农田起垄方向对土壤风蚀具有影响作用 :垄向与风向成 0°时风蚀量最大 ,90°时风蚀量最小 ;在 15m·s-1风速和 3cm表土含水率为 4 5 %的条件下 ,垄向与风向成 0°时的风蚀量为 90°时的 4 8倍。为减轻土壤风蚀危害 ,在河北坝上地区农田起垄的方向宜采用与自然风向NW (西北风向 ,315°)成 90°,即东北方向 4 5°。  相似文献   

运动磁场具有电场的特性——电磁关系之一(英文)   总被引：1，自引：0，他引：1  

陈有君 《内蒙古农业大学学报(自然科学版)》2001,(4)

综合经典电磁学中关于电与磁联系的基本实验定律及理论 ,经推导和分析得出电场与磁场的联系是 :运动磁场具有或表现出电场的特性 ,当一个具有磁感应强度为 B的场 ,以速度 V运动时 ,这个场的电场强度为 :E=B× VB。磁场的时变必然是由于磁场的运动所至 ,而运动的磁场具有电场的性质 ,因此所谓时变磁场产生电场只不过是运动磁场具有电场特性这一自然规律在特定条件下的一种表现。经典电磁学中把磁场分为静磁场和时变磁场是不合理的。因为与静止相应的是运动而不是时变。磁场的时变和磁场的电特性是伴生关系 ,而非因果关系 ,它们都是磁场运动的结果。所以时变磁场产生电场或者 Faraday- L enz定律不是一个普适的自然规律。但在有些条件下 ,运动磁场和其电场特性之间在数量上确实满足 Faraday- L enz定律 ,因此 ,作为一个从实验中总结出的规律 ,并有一定的理论根据 ,它可以在一些领域中作为数学工具用  相似文献