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Responses to anaesthesia with essential oil (EO) of Aloysia triphylla (135 and 180 mg L?1) and tricaine methanesulfonate (MS222) (150 and 300 mg L?1) were assessed in silver catfish. Exposure to the anaesthetics elicited a stress response in the species. In the case of MS222, it was displayed as a release of cortisol into bloodstream, elevation in hematocrit and plasma ion loss. The EO presented cortisol‐blocking properties, but increased haematocrit and disturbances of hydromineral balance were observed. Liver antioxidant/oxidant status of EO and MS222‐anaesthetized silver catfish was also estimated. The synthetic anaesthetic induced lipoperoxidation, notwithstanding increased catalase contents, whereas the naturally occurring product was capable of preventing the formation of lipid peroxides, possibly due to combined actions of catalase and glutathione‐S‐transferase. Anaesthetic efficacy was also tested via induction and recovery times. Overall, the promising results obtained for the physiological parameters of the EO‐treated fish counterbalanced the slight prolonged induction time observed for 180 mg L?1. As for 135 mg L?1, both induction and recovery times were lengthy; despite that, the EO was able to promote oxidative protection and mitigate stress. None of the MS222 concentrations prompted such responses concomitantly.  相似文献   
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The occurrence of pioneer tree species inside tropical forests is usually associated with canopy openness due to disturbances. The distribution of these species under different environmental conditions, aside from light presence, can be influenced by other variables such as soil attributes, water availability, and non-arborous species presence. This work evaluates pioneer tree distribution in the Pindorama Biological Reserve, Brazil, with respect to altitude, soil attributes, and non-arborous species in 65,400-m2 plots in two toposequences of semi-deciduous forest. We evaluated the physical and chemical soil attributes altitude, basal area, height, and number of individuals of tree species with diameter at breast height (DBH) ≥5 cm in a randomly chosen quadrant in each plot. Pioneer trees were characterized by the following higher occurrence species: Acacia polyphylla, Aloysia virgata, Casearia sylvestris, and Croton floribundus. Cluster analysis suggested five similar groups among sampling plots. For each group, the mean of altitude, physical and chemical soil attributes, and degree of non-arborous species infestation was calculated. Principal components analysis correlated variables with pioneer tree data. C. floribundus occurred at low altitudes at lower or higher fertility, C. sylvestris occurred in lower fertility plots, and A. polyphylla and A. virgata occurred in higher altitude plots.  相似文献   
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