Inhibitory Effects of AURKB Gene on Apoptosis and Cancer Cell Growth in HCT 116 Cells     

Wangyuan ZENG  Wenxin DAI 《Medicinal Plant》2019,(6):77-86

[Objectives]To explore the inhibitory effect of AURKB gene in apoptosis and cancer cell growth in HCT 116 cells.[Methods]The in vitro cytology studies were carr...  相似文献   

Effect of beclin-1 gene silencing on TuAKe-injured gastric cancer SGC-7901 cells     

PAN Lei  HU Meng-yi  LI Sai  CHEN Pei-feng  YIN Li-ming 《园艺学报》2019,35(3):436-441

AIM: To observe the effect of beclin-1 silencing by the technique of RNA interference on the injury of human gastric cancer SGC-7901 cell by Sheliugu extract (the extract from tuber of Amorphophallus konjac, TuAKe). METHODS: To knock down the expression of beclin-1 gene, SGC-7901 cells were transfected with lentiviral vector carrying beclin-1-shRNA. The beclin-1 gene knock-down and non-knock-down SGC-7901 cells were treated with TuAKe. The cell viability was analyzed by CKK-8 assay. The percentages of apoptotic cells were detected by flow cytometry. The expression of beclin-1 and LC3 was detected by Western blot. RESULTS: The beclin-1 gene silencing decreased the protein expression of beclin-1 and increased the protein expression of LC3 in the SGC-7901 cells, leading to the decrease in cell viability and the increase in apoptotic rate (P<0.05). TuAKe increased the protein expression of beclin-1 and LC3 in the SGC-7901 cells, and decreased the protein expression of LC3 in the SGC-7901 cells with beclin-1 gene silencing, thus inhibiting the cell viability and increasing the apoptotic rate (P<0.05). CONCLUSION: Beclin-1 gene silencing inhibits the activation of beclin-1-related signaling pathway in gastric cancer SGC-7901 cells, and aggravates the injury of cell viability induced by TuAKe.  相似文献   

miR-204 inhibits proliferation of Hodgkin lymphoma cells by targeting Sirt1/p53 signaling pathway     

ZHENG Xiao-qiang  RUI Hong-bing  ZHANG Guang-hui 《园艺学报》2019,35(9):1557-1564

AIM: To investigate the effect of microRNA-204 (miR-204) on the proliferation of Hodgkin lymphoma cells and the underlying mechanism. METHODS: The expression of miR-204 and Sirt1 mRNA in Hodgkin lymphoma tissues was detected by RT-qPCR. After transfection with miR-204 mimic, Sirt1 siRNA and miR-204 mimic+pcDNA3.1-Sirt1 into the L428 cells, the cell viability and BrdU incorporation were measured by CCK-8 assay and BrdU assay, respectively. The protein levels of Sirt1 and acetylated p53 (ac-p53) were determined by Western blot.The targeting relationship between miR-204 and Sirt1 was verified by double luciferase reporter assay. RESULTS: The low expression of miR-204 and the high mRNA expression of Sirt1 were found in the Hodgkin lymphoma tissues. Compared with control group, the cell viability, BrdU incorporation and the protein levels of Sirt1 and ac-p53 were significantly decreased after L428 cells were transfected with miR-204 mimic or Sirt1 siRNA (P<0.05). Compared with miR-204 mimic alone group, the cell viability, BrdU incorporation and the protein levels of Sirt1 and ac-p53 were increased after L428 cells were co-transfected with miR-204 mimic and pcDNA3.1-Sirt1 (P<0.05). The results of double luciferase reporter assay confiermed that Sirt1 was the target gene of miR-204. CONCLUSION: The inhibitory effect of miR-204 on the proliferation of L428 cells may be achieved by inhibiting the expression of Sirt1 and promoting the up-regulation of ac-p53.  相似文献   

Effect of proline-spirooxindole on viability and apoptosis of non-small-cell lung cancer A549 cells     

MA Jin-zhu  YAN Liang  ZAN Jia-wei  XU Lei  WANG Yi  ZHANG Gen-bao 《园艺学报》2019,35(2):286-290

AIM:To investigate the effect of proline-spirooxindole on the viability and apoptosis of human non-small-cell lung cancer A549 cells. METHODS:The effect of proline-spirooxindole on the viability of A549 cells was determined by CCK-8 assay. The apoptosis was analyzed by flow cytometry. The effects of proline-spirooxindole on the expression of PARP and p53 and the phosphorylation of mTOR were determined by Western blot. RESULTS:After A549 cells were treated with proline-spirooxindole (25, 50 and 100 mg/L), the cell viability was decreased (P<0.01) compared with DMSO control group. The apoptotic rate was increased compared with DMSO control group (P<0.01). The protein expression of p53 was up-regulated, the increased apoptotic protein cleaved PARP was observed, and the phosphorylation of mTOR was inhibited (P<0.01). CONCLUSION:Proline-spirooxindole inhibits the viability of A549 cells and induces apoptosis, which may be related to the phosphorylation of mTOR.  相似文献   

细胞凋亡与疾病   总被引：2，自引：1，他引：1  

王修庚  韦伟  高洪 《中国畜牧兽医》2006,33(9):I0004-I0006

细胞凋亡(apoptos is)是普遍存在于人体组织细胞内的细胞死亡的形式之一,是不同于坏死的正常生理性的程序性细胞死亡。细胞抗凋亡是生物机体为了适应环境变化,维持生理平衡的一种主动的自我保护行为。凋亡或抗凋亡均是在基因控制下,多样性、偶联性、多途径的信号转导过程。它也受到内外生存因子的影响和制约,肿瘤等多种疾病均能影响细胞凋亡过程。  相似文献   

Corneocyte Counts for Evaluation of Antiseborrheic Shampoos in Dogs     

JOHN G. GORDON  KENNETH W. KWOCHKA 《Veterinary dermatology》1993,4(2):57-60

Abstract— An objective method for measuring corneocyte numbers before and after antiseborrheic shampoo therapy was assessed in dogs. Corneocytes were collected from six skin regions on the dorsal trunk of nine colony-raised beagles with clinically normal skin. Forty-eight collection sites were randomly assigned a treatment with one of seven medicated shampoos or water alone; six additional sites served as non-treated controls. Corneocytes were collected immediately before the first treatment, and 14 and 28 days after initiation of therapy with a 0.1% Tween 80 surfactant and a quantitative cup-scrub technique. Pretreatment corneocyte counts were not significantly different among the nine dogs nor among the randomly assigned treatment and control sites (3.55 · 10⁴ cells.cm^-2, SEM 0.17). Corneocyte counts increased in all treatment and control sites from days 0 to 14. Corneocyte counts were similar in the non-treated and water-treated sites from days 14 to 28. Corneocyte counts were not significantly different when the same treatments were compared on days 0, 14 and 28, or when different treatments were compared on the same day. The corneocyte collection technique used in this study proved to be a reliable method for assessing the rate of cell desquamation and cell surface cohesion in dogs.  相似文献   

哺乳动物体细胞克隆研究进展   总被引：3，自引：0，他引：3  

马勇江  曹斌云 《中国牛业科学》2001,27(2):39-41

哺乳动物体细胞克隆技术是近年来才发展起来的技术，但体细胞克隆绵羊－多利的诞生却引起了世界轰动，充分显示了其重在的科学研究价值和潜在的应用价值。本文对哺乳动物体细胞克隆技术研究现状，理论基础研究，应用等方面作一综述。  相似文献   

Caspase非依赖性细胞死亡的研究进展   总被引：2，自引：0，他引：2  

宋佰芬  崔玉东  曹红伟 《吉林畜牧兽医》2005,(5):10-13

细胞凋亡是一种生理性细胞死亡机制，它是由多基因调控的一种细胞的主动的自杀性过程。一般认为它是由蛋白酶caspases通过两种途径激活的。但是，近年来研究发现除caspases引起的细胞凋亡外，还有caspases非依赖性的细胞死亡形式存在，包括细胞的自我吞噬作用、细胞蛋白酶体的降解和线粒体途径。这些途径无论是在试验的条件下还是在生理或病理的情况下都可以观察到。目前认为凋亡诱导因子(AIF)在caspases非依赖性的细胞死亡中起关键作用。  相似文献   

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro     

CAI Yun-feng  MIN Jun  FANG Tian-ling  CHU Zhong-hua  DENG Xiao-geng  HE Jing-song  CHEN Ji-sheng 《园艺学报》2003,19(8):1029-1033

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.  相似文献   

羊肚菌研究进展   总被引：29，自引：1，他引：29  

陈向东  朱戎  兰进 《食用菌学报》2002,9(2):56-61

综述了近年来羊肚菌营养成分、有效成分，培养技术，以及细胞学、生物化学和分子生物学等方面的研究进展。  相似文献