A Case of Gastric Adenocarcinoma in a Shih Tzu Dog: Successful Treatment of Early Gastric Cancer     

Hee-Chun LEE  Ji-Hyun KIM  Cho-Hee JEE  Jae-Hoon LEE  Jong-Hyun MOON  Na-Hyun KIM  Jung-Hyang SUR  Kyu-Woan CHO  Byeong-Teck KANG  Jeongim HA  Dong-In JUNG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(7):1033-1038

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依葡柠解冻液对种公牛精子寿命及完整顶体率的影响     

李巧丽  易建明  熊家军  祁明德  程先菊 《中国兽医学报》2002,22(4):405-406

采用复方依葡柠解冻液 (EDTA 葡萄糖 柠檬酸钠 ,试验组 )与常用的单方柠檬酸钠解冻液 (2 .9%柠檬酸钠 ,对照组 )对肉用种公牛颗粒冻精进行解冻对照试验。结果显示 ,解冻后精子的复苏率 ,试验组与对照组相差不大 (P >0 .0 5 ) ;常温 (18～ 2 0℃ )和低温 (5～ 7℃ )下再贮存 ,精子的存活时间和生存指数 ,试验组高于对照组 ,且差异显著 (P<0 .0 5 ) ;解冻后在 (38± 2 )℃水浴中孵育 3h检查精子的完整顶体率 ,试验组极显著高于对照组 (P<0 .0 1)。适用于水牛的良好解冻液依葡柠 ,用做牛 (肉用牛 )的冻精解冻得到了理想效果  相似文献   

利用响应面法优化茶叶中EGC的浸提条件     

王贤波  崔红春  黄海涛 《茶叶》2011,37(4):221-224

用去离子水浸提茶叶中的EGC,用高效液相色谱仪检测EGC并计算浸出率。在单因素实验基础上,利用Box—Benhnken实验设计进行三因素三水平的响应面分析实验,获得最优浸提条件：料液比1：12、pH6．2、温度78℃、时间18min、浸提2次。  相似文献   

Comet assay in gill cells of Prochilodus lineatus exposed in vivo to cypermethrin     

G.L. Poletta  F. Gigena  A. Loteste  M.J. Parma  E.C. Kleinsorge  M.F. Simoniello 《Pesticide biochemistry and physiology》2013

Agricultural chemicals can induce genetic alterations on aquatic organisms that have been associated with effects on growth, reproduction and population dynamics. The evaluation of DNA damage in fish using the comet assay (CA) frequently involves the utilization of erythrocytes. However, epithelial gill cells (EGC) can be more sensitive, as they are constantly dividing and in direct contact with potentially stressing compounds from the aquatic environment. The aim of the present study was to evaluate (1) the sensitivity and suitability of epithelial gill cells of Prochilodus lineatus in response to different genotoxic agents through the application of the CA, (2) the induction of DNA damage in this cell population after in vivo exposure to cypermethrin. Baseline value of the CA damage index (DI) for EGC of juvenile P. lineatus was 144.68 ± 5.69. Damage increased in a dose-dependent manner after in vitro exposure of EGC to methyl methanesulfonate (MMS) and H₂O₂, two known genotoxic agents. In vivo exposure of fish to cypermethrin induced a significant increase in DNA DI of EGC at 0.150 μg/l (DI: 239.62 ± 6.21) and 0.300 μg/l (270.63 ± 2.09) compared to control (150.25 ± 4.38) but no effect was observed at 0.075 μg/l (168.50 ± 10.77). This study shows that EGC of this species are sensitive for the application of the CA, demonstrating DNA damage in response to alkylation (MMS), oxidative damage (H₂O₂), and to the insecticide cypermethryn. These data, together with our previous study on DNA damage induction on erythrocytes of this species, provides useful information for future work involving biomonitoring in regions where P. lineatus is naturally exposed to pesticides and other genotoxic agents.  相似文献