水产动物6种主要病原菌与抗血清的免疫交叉反应   总被引：14，自引：0，他引：14       下载免费PDF全文

战文斌  齐继光  刘洪明  周丽  邢婧 《中国水产科学》2004,11(1):14-19

采用ELISA、试管凝集、Western-blot等方法,分析了鳗弧菌(Vibrio anguillarum)、哈维氏弧菌(V. harveyi)、溶藻胶弧菌(V. alginolyticus)、副溶血弧菌(V. paraheamolyticus)、迟钝爱德华氏菌(Edwardsiella tarda)和荧光假单胞菌(Psedomonas fluorescens)等水产养殖中主要病原细菌与抗血清之间的免疫交叉反应.结果表明,弧菌属细菌之间的交叉反应程度比较大,而与其他两属的细菌之间存在的交叉反应程度小,或不存在交叉反应;Western-blot分析结果显示,哈维氏弧菌、溶藻胶弧菌和副溶血弧菌抗血清分别与其他3种弧菌在分子量为135.6 kD和121.5 kD;95.6 kD,48.4 kD,39.2 kD和34.9 kD;55.1 kD的蛋白带处存在交叉反应,而这些分子量的蛋白带与其他两属的抗血清均不发生反应.  相似文献   

Blood parameters in Nile tilapia (Oreochromis niloticus L.) spontaneously infected with Edwardsiella tarda   总被引：6，自引：0，他引：6  

A Ca&#;lan Karasu Benli  & Hijran Yavuzcan Yildiz 《Aquaculture Research》2004,35(14):1388-1390

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迟缓爱德华菌(Edwardasiella tarda)对小鼠和剑尾鱼的免疫保护试验   总被引：3，自引：0，他引：3  

熊清明  陆承平 《中国兽医学报》2002,22(3):251-253

为了评价迟缓爱德华菌 (Edwardasiella tarda)的免疫保护效果 ,用致病性 E.tarda JEL4的全菌灭活物 (FKC)和胞外产物 (ECP)分别 2次免疫小鼠 15 0只和 1次免疫剑尾鱼 30 0尾 ,7周后用同源菌株和异源菌株进行攻击。结果显示 ,除 ECP组对某一异源菌株为 4/ 5保护外 ,免疫小鼠 FKC组和 ECP组全部获得保护 (5 / 5 )。在免疫的剑尾鱼中 ,FKC和 ECP组对同源菌株保护率分别为 6 0 %和 10 0 % ;对异源菌株的攻击 ,均不能全部保护 ,但 ECP组可明显延缓剑尾鱼死亡时间  相似文献   

地图鱼迟钝爱德华氏菌病病原菌的鉴定及毒力基因的检测   总被引：3，自引：0，他引：3  

赵飞  邹为民  谭爱萍  罗理  姜兰  陆小萏 《大连水产学院学报》2007,22(6):403-408

从患病地图鱼Astronotus ocellatus肝脏组织中分离到菌株AO1,经人工感染试验证实其为致病菌。通过ATB Expression半自动细菌鉴定仪和常规生理生化特性的分析,初步鉴定该菌株为迟钝爱德华氏菌Ed-wardsiella tarda。测定菌株AO1的16S rRNA基因序列并进行分析,结果显示,该菌株与迟钝爱德华氏菌的同源性最高,达99.9%。因此,可鉴定该菌株为迟钝爱德华氏菌。根据迟钝爱德华氏菌的毒力基因菌毛亚基(fimA)基因序列设计引物,进行PCR扩增,得到383 bp序列,该序列与迟钝爱德华氏菌的fimA基因序列相似性达99.0%,进一步说明菌株AO1具有fimA基因,有一定的致病力。药物敏感试验结果表明,菌株AO1对阿米卡星、氧氟沙星、庆大霉素、新生霉素、氨苄西林、氨曲南、卡那霉素、头孢咪啉、诺氟沙星、环丙沙星、呋喃妥因、妥布霉素、氨苄青霉素13种抗生素较为敏感。  相似文献   

迟缓爱德华氏菌检验程序的研究   总被引：10，自引：0，他引：10  

葛艳  陈怀青  陆承平 《中国动物检疫》1999,16(3):2-4

本试验研究了迟缓德华氏菌（Edwardsiellatrada,Et）的细菌学及致病因子检测,确定了相应检测指标,制定了检验程序。对不同来源的Et作分离及生化鉴定,确定10项生化鉴定指标。用三种方法即平板法（PlateAssay,PA）、接触法（ContactHemolysis,CH）和上清法（SunernatantAs－say,SA）检测Et溶血素,阳性率依次为75％、75％、57．14％。28株Et中,18／26的胞外产物（Extra－cellularProducts,ECP）对HEP－2细胞有细胞毒性,17／28菌株有侵袭力。用ATCC15947株ECP的抗血清进行Det－ELISA,检测阳性率为19／26（73．08％）。  相似文献   

Edwardsiella tarda infection in Korean catfish,Silurus asotus,in a Korean fish farm     

Jin‐Ha Yu  Jung Jo Han  Kwon Sam Park  Kwan Ha Park  Sung Woo Park 《Aquaculture Research》2009,41(1):19-26

Mass mortality of Korean catfish, Silurus asotus, occurred in a culture farm situated in Jeollabukdo Province, Korea. The cumulative mortality rates reached up to 5% of the total fish in the farm per day. In clinical signs, the affected fish showed abdominal distension, vent protrusion, enteritis, liver congestion and abscess‐like lesions in enlarged spleen and kidney. Histopathologically, in the liver, hepatocytes lost fat and underwent atrophy or necrosis. The spleen showed necrotized splenocytes and a haemorrhagic pulp. In the kidney, glomerular destruction, degeneration of renal tubular epithelial cell and haemorrhage were observed. However, necrotic muscular lesions were not observed. A pure bacterial isolate was obtained from the liver, spleen and kidney lesions of affected fish. Experimental infection of normal catfish with the isolate resulted in the development of clinical signs similar to those seen on the farm. The isolates were identified as Edwardsiella tarda through biochemical tests (99.4%) and analysis of bacterial genes (16S rDNA) sequences (98%). The bacteria possessed two virulent genes: sodB and katB genes. These results suggest that E. tarda can act as a pathogen of farmed catfish. This is the first report showing that E. tarda caused mortality in cultured Korean catfish.  相似文献   

Surface-exposed expression of Edwardsiella tarda EseB in live attenuated Vibrio anguillarum based on novel surface display systems     

Qiyao Wang  Zhao Yang  Qin Liu  & Yuanxing Zhang 《Aquaculture Research》2009,40(13):1459-1467

Live, attenuated Vibrio anguillarum strains can serve as vectors for the delivery of heterologous antigens for development of multivalent recombinant vaccines. Based on the outer membrane anchoring elements of V. anguillarum , we have previously constructed several efficient surface display systems Lpp-Omporf1, Lpp-OmpU, Lpp-Omp26La, Wza-Omporf1, Wza-OmpU and Wza-Omp26La. In this study, with these constructed surface display systems, a putative antigen protein EseB from pathogenic Edwardsiella tarda was successfully expressed on the surface of an attenuated V. anguillarum strain to get multivalent vaccine candidates. Further immune protection evaluation in zebra fish ( Danio rerio ) demonstrated that the V. anguillarum EseB-display strain AV/pW-26La-B could trigger full protection against V. anguillarum infection and early protection against E. tarda infection in the immunized fish. These results suggest that surface display of heterologous protective antigens in attenuated V. anguillarum could be used as a tool to develop potential V. anguillarum vector vaccine.  相似文献   

河鲈细菌性肠炎致病菌的分离、鉴定及药敏实验          下载免费PDF全文

杨昆明  郭爱民  马江霞  段成任  谢志胜  岳城 《渔业科学进展》2018,39(6):42-51

河鲈(Perca fluviatilis Linnaeus)是新疆额尔齐斯河特有的土著鱼类,已开展人工养殖,但其病害相关研究较少。2016年,五家渠某水产推广站河鲈突然大批死亡,作者从发病的河鲈肝脏和肠道分离病原菌,对分离菌进行了传代纯化培养。结果显示,在20℃培养24 h后,ZL1能形成边缘整齐、中间微隆起、表面光滑、浅黄色、有特殊芳香气味、大小为1.5~2 mm的菌落,在血琼脂平板上能形成明显的β-溶血圈;ZL3和ZL5可形成圆形、微隆起、表面湿润光滑、灰白色、半透明、菌落大小为0.5~ 1 mm的菌落,但在血琼脂平板上不能形成溶血圈。经革兰氏染色、生理生化、16S rRNA序列比对和基因序列进化树分析,鉴定ZL1株为温和气单胞菌(Aeromonas sobria),ZL3和ZL5株为迟缓爱德华菌(Edwardisella tarda)。药敏实验结果显示,ZL1株对庆大霉素、米诺环素、头孢他啶等药物高度敏感,对链霉素、青霉素、克拉霉素等耐药;ZL3和ZL5株对左氧氟沙星、链霉素和恩诺沙星敏感,对庆大霉素、氨苄西林、青霉素、复方新诺明等药物耐药。人工感染结果显示,分离菌均具有明显致病性。  相似文献   

Occurrence of multiple antibiotic resistance and genotypic characterization in Edwardsiella tarda isolated from cage‐cultured hybrid red tilapia (Oreochromis sp.) in the Ping River,Northern Thailand     

Guoyi Niu  Dilok Wongsathein  Sukolrat Boonyayatra  Rutch Khattiya 《Aquaculture Research》2019,50(12):3643-3652

Edwardsiella tarda is a pathogen that causes edwardsiellosis in aquatic animals. The emergence of multiple antibiotic‐resistant strains makes antibiotic treatment difficult. This study aimed to investigate the antibiotic susceptibility patterns and the genotypic characterization of E. tarda isolated from cage‐cultured red tilapia in Thailand. A total of 30 isolates were identified as E. tarda using biochemical and molecular analysis. The disc diffusion method for testing antibiotic susceptibility showed all the isolates were resistant to colistin sulphate and oxolinic acid. High levels of resistance to amoxicillin, ampicillin, ceftazidime, oxytetracycline and sulphamethoxazole/trimethoprim were observed as well. The multiple antibiotic resistance index ranged from 0.25 to 0.92, indicating that these isolates had been exposed to high risk sources of contamination where antibiotics were commonly used. All the isolates carried the bla_TEM gene based on polymerase chain reaction (PCR). The tetA and sul3 genes were detected in 90% (27/30) and 26.7% (8/30) of the isolates respectively. Nine different genetic groups of isolates were obtained using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC‐PCR). A correlation between genetic types and multiple antibiotic‐resistant patterns was found. These results highlight the potential risks of multiple antibiotic‐resistant isolates for humans and the environment.  相似文献   

Specific MHC class II B alleles associated with resistance to Edwardsiella tarda in turbot, Psetta maxima (L.)     

J-Y Xu  S-L Chen  H Ding 《Journal of fish diseases》2009,32(7):637-640

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