全文获取类型
收费全文 | 1484篇 |
免费 | 65篇 |
国内免费 | 78篇 |
专业分类
林业 | 83篇 |
农学 | 92篇 |
基础科学 | 214篇 |
235篇 | |
综合类 | 617篇 |
农作物 | 95篇 |
水产渔业 | 32篇 |
畜牧兽医 | 129篇 |
园艺 | 110篇 |
植物保护 | 20篇 |
出版年
2024年 | 8篇 |
2023年 | 24篇 |
2022年 | 21篇 |
2021年 | 31篇 |
2020年 | 23篇 |
2019年 | 27篇 |
2018年 | 21篇 |
2017年 | 33篇 |
2016年 | 63篇 |
2015年 | 70篇 |
2014年 | 92篇 |
2013年 | 96篇 |
2012年 | 115篇 |
2011年 | 116篇 |
2010年 | 80篇 |
2009年 | 101篇 |
2008年 | 91篇 |
2007年 | 108篇 |
2006年 | 81篇 |
2005年 | 76篇 |
2004年 | 54篇 |
2003年 | 42篇 |
2002年 | 34篇 |
2001年 | 28篇 |
2000年 | 29篇 |
1999年 | 24篇 |
1998年 | 17篇 |
1997年 | 17篇 |
1996年 | 21篇 |
1995年 | 14篇 |
1994年 | 15篇 |
1993年 | 14篇 |
1992年 | 7篇 |
1991年 | 10篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1986年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有1627条查询结果,搜索用时 15 毫秒
1.
为提高天麻栽培产量,扩大天麻栽培地域,提高山区有限土地利用率,笔者将影响天麻田间高产栽培的几个关键因子进行比较试验,总结出天麻田间高产栽培的有效措施,为大力发展天麻生产提供了科学依据。 相似文献
2.
AIM:To investigate expression and function of CD40 ligand by peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE).METHODS:Expression of CD40 ligand by PBMCs in patients with SLE and control were examined by flow cytometric analysis before and after stimulated by phytohemagglutinin(PHA)and depressed by Dexamethasone(Dex). The correlation between expression of CD40 ligand and SLE activity index(SLEDAI) was analysed in patients with SLE.RESULTS:The expression of CD40 ligand by PBMCs in patients with active SLE was higher than that in patients with inactive SLE and control. Though the expression of CD40 ligang by PBMCs could be stimulated by PHA in three groups, it was the highest in patients with active SLE. Dex depressed the expression of CD40 ligand by PBMCs significantly in patients with SLE, but not in control. There was high positive correlation between expression of CD40 ligand and SLEDAI in patients with active and inactive SLE.CONCLUSION:Increased expression of CD40L by PBMCs in patients with SLE may play an important role in pathogenesis of SLE. 相似文献
3.
AIM: To study the effect of cocaine on caspase-3 in myocardiac cells of male rats in different age. METHODS: Three-week-old(n=16), six-week-old(n=16) and twelve-week-old (n=16) male Sprague-Dawley rats were all divided into control groups and experiment groups randomly, each group had eight animals, experiment groups were given cocaine hydrochloride (15 mg·kg-1 body weight) subcutaneously daily for four weeks. The ratio of heart weight to body weight (HW/BW, mg/g) were measured. DNA fragmentation of myocardia cells was determined by gel electrophoresis, and caspase-3 activity in myocardia cells was tested by flow cytometry (FCM). RESULTS: In three experiment groups, the DNA isolated from myocardial cells displayed clear ladder pattern. The HW/BW and the caspase-3 activity were increased significantly than those of control groups (P<0.01). CONCLUSIONS: Cocaine induced apoptosis in rat myocardial cells. The increase in caspase-3 activity may be the one of important pathways related to the induction of apoptosis. 相似文献
4.
AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P<0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps. 相似文献
5.
AIM: To investigate the relationship between somatostatin and the pathologic type, estrogen receptor,DNA ploidy of nuclei in tumor cells of breast cancer.METHODS: 67 cases of primary breast cancer and 25 cases of benign breast tumor were examined by immunohistochemical stretomyces avidin peroxidase method. 26 cases of breast cancer selected at random were analyzed by flow cytometry.RESULTS: Somatostatin expressed significantly higher in low malignant breast cancer than that in high malignant breast cancer (P<0.05). Most of cancers with positive staining of somatostatin were diploidy,most of cancers with negative staining were aneuploidy,there had significant difference between two groups (P<0.05).CONCLUSION: Somatostatin may delay the progress of breast cancer,and somatostatin levels in cancer tissues may become a useful indicator for assessing prognosis of patients with breast cancer. 相似文献
6.
张晓敏 《上海交通大学学报(农业科学版)》2015,(2):120-122
采用了文献资料法、问卷调查法、数理统计法、逻辑分析法对影响我国大学城体育场地资源共享的因素进行了分析与总结,研究结果表明,影响大学城高校体育场地资源共享的主要因素包括:场地客观因素、学校领导因素、学校教师因素、学生因素、地理位置因素、相关政策因素、课程安排因素、经济因素、社会因素、比赛安排因素。根据木桶效应,在构建大学体育场地资源共享模式的时候,需要对课程进行合理的安排,并做好场地对内和对外开放的时间以及方式合理安排,以提供我国高校体育场地资源的利用率,促进我国高等体育教育和社会体育的发展。 相似文献
7.
8.
9.
10.
Routine agricultural practices are heavily dependent on the use of surfactants, many of which are toxic to humans and detrimental to the environment. In proof of concept work we have previously shown the potential of nanostructured liquid crystalline particles (NLCP) to safely interact with plant leaf cuticular surfaces with minimal impact on epicuticular waxes. Here we demonstrate the use of NLCP to effectively deliver the auxin herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) to plant leaves in laboratory and field studies. In the laboratory, the physiological stress responses of lupin, Lupinus angustifolius (L.) (Fabaceae) towards NLCP spray applications were shown to be much reduced in comparison with application of two common surfactants. Phytotoxicity assays of 2,4-D loaded NLCP were used to validate the herbicidal effects on Arabidopsis thaliana (L.) Heynth. (Brassicaceae) and established a similarity with that of surfactant assisted 2,4-D delivery when tested at a concentration of 0.1%. Field trials were conducted to test the efficacy of NLCP-assisted delivery of 2,4-D in comparison with commercial surfactants for the control of the invasive weed wild radish, Raphanus raphanistrum (L.) (Brassicaceae), in wheat, Triticum aestivum (L.) (Poaceae) crop fields. Compared against Estercide 800, a commercially available 2,4-D formulation, NLCP assisted delivery of 2,4-D was effective at low concentrations of 0.03% and 0.06%. The crop yield remained similar for all the tested concentrations and formulations of 2,4-D loaded NLCP and Estercide 800. This is the first report to directly show that, as an alternative to conventional methods, NLCP can be used under both laboratory and field conditions to successfully delivery an agrochemical. 相似文献