真蛸人工繁殖及受精卵孵化技术研究初报     

钟建兴  李雷斌  宁岳  刘波  曾武斌 《福建水产》2011,33(5):39-42

真蛸为福建省近年来新兴的海水养殖优良种类,具有较高的营养价值、商品价值和产业化养殖前景。本文报道了福建省水产研究所2008年-2011年真蛸人工繁殖及受精卵孵化结果：127头雌亲蛸经强化培育,共有77头产卵,获卵194×10^4粒,孵出143．4×10^4头浮游幼体。雌亲蛸获产率46．2％～86．7％,卵子受精率75．6％-91．8％,受精卵孵化率77．9％～95．0％。在20．0～22．5℃水温条件下,真蛸受精卵经21～36d开始孵出,孵出后4～6d为孵出高峰,每窝卵开始孵出至孵化结束维持10—16d。  相似文献   

Analysis of bacterial community and bacterial nutritional enzyme activity associated with the digestive tract of wild Chilean octopus (Octopus mimus Gould, 1852)          下载免费PDF全文

Shunpei Iehata  Fernando Valenzuela  Carlos Riquelme 《Aquaculture Research》2015,46(4):861-873

Information on the bacterial community associated with octopus is very scarce, unlike fish and other molluscs. This study revealed the bacterial community associated with digestive tract of wild Chilean octopus Octopus mimus using a culture‐dependent method and 16S rDNA clone library. Moreover, we analysed the bacterial nutritional enzyme activity of culturable bacteria. A culture‐dependent method showed that the composition of the culturable bacterial community was substantially different between female and male octopus. The predominant species in female octopus were Vibrionaceae and Streptococcaceae, whereas only Vibrionaceae was dominated in male octopus. Bacterial nutritional enzyme activities of culturable bacteria from male octopus were much higher than female octopus. The 16S rDNA clone library analysis showed that the bacterial community of male octopus exhibited a higher diversity than that of female octopus. The genus Mycoplasma was the predominant bacteria in the digestive tract of all octopus samples. The results obtained in this study raise the possibility that each octopus has different food consumption due to different bacterial community and nutritional enzyme activity, although Mycoplasma sp. is one of the predominant bacteria in the digestive tract. Moreover, our results are useful for the future of microbiological investigation associated with the octopus and for probiotics in the octopus aquaculture.  相似文献   

美国红鱼养殖种群的同工酶酶谱及其生化遗传初步分析   总被引：6，自引：0，他引：6  

刘世禄  王波  高天翔  柳广东  张锡烈 《海洋水产研究》2002,23(3):10-14

利用水平淀粉凝胶电泳技术，对50尾美国红鱼肌肉及肝脏组织进行了14种同工酶的初步分析。结果表明，美国红鱼多态位点比例P＝0.292，实际平均杂合度Hp=0.057，预期杂合度He=0.108，每个位点的有效等位基因数Ae=1.108，Hardy-Weinberg遗传偏离指数D＝－0.472。  相似文献   

美国红鱼继饥饿后的补偿生长及其机制   总被引：61，自引：8，他引：61  

姜志强 《水产学报》2002,26(1):67-72

对两种不同规格 (8.2 5± 0 .5 0g ,4 0 .4 1± 2 .4 5g)的美国红鱼分别在 18～ 2 0℃和 2 0～ 2 4℃条件下进行了不同饥饿时间处理后再恢复投喂实验。在实验的 30d内 ,两次实验结果均表现为饥饿 5d组的美国红鱼生长略快于对照组 ,而饥饿 10d和 15d的鱼在相同的时间内 ,体重的增加量未能赶上对照组。各组间生长虽有差别 ,但都未达到显著水平。饥饿使鱼体水分、灰分含量增加 ,脂肪含量下降 ,蛋白质含量变化不大 ,但各成分从恢复投喂到实验结束时又恢复到对照组水平。各组间摄食量随饥饿时间延长而逐步下降 ,而饵料转换效率逐步上升。随着饥饿时间的延长 ,标准代谢率下降 ,而在恢复投喂之后又逐步回升至对照组水平。结果表明 ,美国红鱼的补偿生长效应主要是由降低标准代谢和提高食物转化率实现的  相似文献   

高体鳑鲏幼鱼集群行为的研究     

覃英莲  柏杨  彭姜岚  李秀明  付世建 《水生态学杂志》2017,38(4):71-76

为了考查高体鳑鲏(Rhodeus ocellatus)幼鱼的集群行为,研究选取248尾幼鱼,组成1、2、4、8和16尾高体鳑鲏幼鱼的群体(N = 8),在(25 ± 1)℃水温条件下进行15分钟的视频拍摄、运动轨迹提取和分析。结果显示：①高体鳑鲏的自发游泳速度多介于0-10 cm/s之间,其速度中位数随群体大小上升呈先下降后上升的趋势(P ﹤ 0.05),但各组间游泳速度的同步性随群体大小的上升而下降(P ﹤ 0.05);②焦点鱼和群体中其它个体的最近邻距离(NND)多出现在0-10 cm之间,且2和4个体组的NND显著大于8和(或)16个体组(P ﹤ 0.05);③2和4个体组排列的极性显著高于8和16个体组(P ﹤ 0.05)。研究结果表明群体大小对高体鳑鲏集群行为的特征有显著影响,随群体大小上升,凝聚力上升,但协调性有所下降。  相似文献   

Trawling for cephalopods off the Portuguese coast—Fleet dynamics and landings composition   总被引：1，自引：0，他引：1  

Tereza Fonseca  Aida Campos  Manuel Afonso-Dias  Paulo Fonseca  Joo Pereira 《Fisheries Research》2008,92(2-3):180-188

Fleet dynamics was addressed for three cephalopod taxa of commercial interest, the squid Loligo vulgaris, the octopuses Octopus vulgaris and Eledone cirrhosa, and the cuttlefish Sepia officinalis, for 48 trawlers of the fish trawling fleet. Landing profiles (LP) were identified based on the species composition of the landings using hierarchical cluster analysis. Four out of a total of 12 different LP were related to cephalopods and other species associated with them.The effects on the landing proportions of a number of variables, year, season and vessel, are analysed for each of the species studied using generalized linear models (GLM). The factor “vessel”, including an ensemble of technical characteristics as well as the abilities of individual skippers, explained most of the model deviance, strongly reinforcing the existence of a fleet component dedicated to catch cephalopods. However, time also explains much of the variation found in the data.Seasonal alternation between landings of octopodidae and cuttlefish was observed within a small group of old trawlers operating mainly off the south coast, following the abundance cycles of these species. For a larger group of more modern trawlers, operating off the western coast, inter-annual shift between octopus and squid was found, together with a well marked seasonal pattern between the catches of cephalopods and horse mackerel.Spatial patterns of activity were identified using vessel monitoring system (VMS) data available for trawlers in Portugal, demonstrating the existence of cephalopod targeting strategies in Portuguese fish trawling activities.  相似文献   

真蛸人工繁殖初步试验   总被引：4，自引：2，他引：4  

林国清  黄健  胡荣炊  宋武林 《福建水产》2008,(3)

2005年春、秋两季在福建省大成水产良种繁育试验中心开展了真蛸(Dctopus vulgaris)人工繁殖初步试验.引进本地海区真蛸亲体48头,获得受精卵216万粒,孵化出幼体139万头,在春季成功培育着底幼蛸121头.开口饵料配置试验表明,卤虫无节幼体、轮虫、桡足类可作为真蛸幼体的开口饵料,以卤虫无节幼体为最佳.  相似文献   

地图鱼迟钝爱德华氏菌病病原菌的鉴定及毒力基因的检测   总被引：3，自引：0，他引：3  

赵飞  邹为民  谭爱萍  罗理  姜兰  陆小萏 《大连水产学院学报》2007,22(6):403-408

从患病地图鱼Astronotus ocellatus肝脏组织中分离到菌株AO1,经人工感染试验证实其为致病菌。通过ATB Expression半自动细菌鉴定仪和常规生理生化特性的分析,初步鉴定该菌株为迟钝爱德华氏菌Ed-wardsiella tarda。测定菌株AO1的16S rRNA基因序列并进行分析,结果显示,该菌株与迟钝爱德华氏菌的同源性最高,达99.9%。因此,可鉴定该菌株为迟钝爱德华氏菌。根据迟钝爱德华氏菌的毒力基因菌毛亚基(fimA)基因序列设计引物,进行PCR扩增,得到383 bp序列,该序列与迟钝爱德华氏菌的fimA基因序列相似性达99.0%,进一步说明菌株AO1具有fimA基因,有一定的致病力。药物敏感试验结果表明,菌株AO1对阿米卡星、氧氟沙星、庆大霉素、新生霉素、氨苄西林、氨曲南、卡那霉素、头孢咪啉、诺氟沙星、环丙沙星、呋喃妥因、妥布霉素、氨苄青霉素13种抗生素较为敏感。  相似文献   

嘉庚蛸雄性生殖系统组织学观察   总被引：3，自引：0，他引：3  

焦海峰  施慧雄  尤仲杰 《上海海洋大学学报》2010,(3)

应用组织切片技术对浙江沿海自然分布的嘉庚蛸雄性生殖系统的结构变化进行了逐月研究,结果发现,雄性生殖系统由单个精巢、输精管、储精囊、前列腺、精荚囊和阴茎等6部分构成。精巢球形,辐射型精巢,精原细胞椭圆或近圆形,直径10～16μm,精子细胞经过椭圆形梭型柳叶型长棒型的变态期,成为鞭毛型精子,精子头部细长等粗棍状,长约26～33μm,尾部长度约为头部的16倍。输精管长约13～16cm,是雄性生殖系统中最细长的管道。储精囊长约5～8cm,可分为储精管和腺体2部分。前列腺与储精囊相接共同开口于精荚囊腔,中空盲管状,4.8～5.6cm。精荚囊分为2部分,一侧为叶脉状规则褶皱突起围成的腺体腔,称之为囊内腺,另一侧突起少,个体大、不规则,树枝状。阴茎短,仅2～3cm,生殖孔开口于胴体左侧外套腔的中部。储精囊、前列腺和精荚囊中均存在着具有分泌功能的上皮构成的腺体腔,它们在精荚成熟装配过程中发挥着不可取代的作用。阴茎中段的阴茎囊呈镜面倒卜型,它不仅是分泌性的腺体腔,而且还是精荚排出过程中的反转场所。  相似文献   

真蛸孵化过程的初步研究     

张宇  赵盛龙  张振伟 《浙江水产学院学报》2010,(4):346-350

2007年4月-2008年5月,在嵊山后头湾养殖场进行了真蛸生态育苗试验,研究发现,不同成熟度的真蛸亲体,在卵巢的形状、大小以及卵细胞形态、色泽上有明显差异,并据此可划分为发育间期、成熟期和临产期3个时期。而孵化过程则可分为卵裂初期、＂红珠＂期、＂黒珠＂期及幼蛸出膜期4个阶段。在水温23-28℃、盐度28-31下孵化,约需32 d,孵化过程中受精卵的大小变化不明显,亲体有极强的护卵习性。  相似文献