Effect of the Pietrain breed used as terminal boar on Post-weaning Multisystemic Wasting Syndrome (PMWS) in the offspring in four PMWS-affected farms     

Nicolas Rose  Alain Abhervé-Guéguen  Gérald Le Diguerher  Eric Eveno  Jean-Pierre Jolly  Philippe Blanchard  Aurélie Oger  André Jestin  François Madec 《Livestock Production Science》2005,95(3):177-186

The aim of this study was to determine the potential risk factors for PMWS at the individual pig level and assess the effect of the Pietrain paternal genetic background of the animals in a cohort study. The survey was set up in four PMWS-affected farms with 2 repetitions (batches) per farm. A representative sample of 60 pigs per batch, stratified according to the paternal genetic background (Pietrain: yes vs. no), was randomly selected after farrowing. The representative cohort was divided into 8 batches and the pigs were individually monitored from birth to slaughter. Survival analysis was used to determine the factors related to the time to PMWS. The litter-cluster effect was taken into account using the marginal Cox model (robust estimation of the covariance matrix) and the gamma shared frailty model which were compared.No protective effect of the Pietrain breed on the time to PMWS and the proportion of affected pigs in the offspring was found in this study. Piglets showing low circovirus type 2 (PCV2) titres at 7 weeks-old with no subsequent seroconversion and piglets from PCV2 negative sows were most likely to be affected by PMWS (HR=7.0 and 2.8, respectively). Active infection of the pregnant dams with parvovirus was related to an increased risk of PMWS in the offspring (HR=2.3). Neck injuries due to poorly performed injections in the dams were associated with an increased risk of PMWS with the marginal model (HR=2.1). Oxytocin injection (dams) during farrowing was protective against PMWS in the offspring (HR=0.6).  相似文献   

PRRS ELISA试剂盒检测猪繁殖与呼吸综合征病毒抗体的应用   总被引：2，自引：0，他引：2  

吴延功王志亮  王君玮徐天刚张喜悦  宋翠平许立华 陈溥言 《中国动物检疫》2004,21(6):23-25

利用重组杆状病毒表达的PRRSV核衣壳蛋白作抗原制成ELISA诊断试剂盒，检测人工感染PRRSV猪血清、疫苗免疫抗体和田间血清，并与IDEXX公司生产的试剂盒进行比较。结果表明，该试剂盒在PRRSV感染后8天内就可检出感染性抗体，而用IDEXX公司生产的试剂盒在感染后的18天才检测到感染性抗体，对弱毒疫苗的免疫检测也基本能反映出疫苗的免疫应答状况。对华东地区PRRSV流行病学调查结果表明，PRRSV在国内普遍存在，同时也证明研制的试剂盒，是客观科学调查我国PRRS流行情较理想的检测工具。  相似文献   

肉仔鸡低血糖-尖峰死亡综合征的流行病学分析和病原初探     

吴延功梁万友  徐书永王志亮 魏荣陈正平  张德新 《中国动物检疫》2005,22(8):20-22

自2003年6月份以来，在我国北方肉鸡饲养基地普遍流行了一种主要侵害肉仔鸡的疾病，低血糖-尖峰死亡综合征(HSMS)。HSMS发病日龄集中于10～18日龄．临床表现为突然出现的高死亡率，病鸡头部震颤、运动失调、昏迷、失明、死亡。先后对黑龙江、吉林、辽宁、内蒙古、天津、河北、山东等省进行调查．发现HSMS在这几个省的很多肉鸡饲养区广泛存在。对发生该病的259个肉鸡饲养户的调查结果显示．该病与祖代种鸡没有关系．病原可能来自受污染的父母代种鸡场。平均发病率为17．9％。发病鸡群血糖浓度极显著的下降。从2个发病鸡群的病鸡肠道内容物中观察到病毒颗粒．病毒呈多形性．有囊膜，直径约80-100nm，表面有棒状纤突，长约10-20nm．与冠状病毒类似。  相似文献   

Effect of Age,Season, Body Condition,and Endocrine Status on Serum Free Cortisol Fraction and Insulin Concentration in Horses          下载免费PDF全文

K.A. Hart  D.M. Wochele  N.A. Norton  D. McFarlane  A.A. Wooldridge  N. Frank 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(2):653-663

  相似文献   

猪繁殖-呼吸综合征病毒上海株的分离鉴定   总被引：7，自引：0，他引：7  

严亚贤  孙建和  王恒安 《中国预防兽医学报》2000,22(3):161-163

从上海地区５个发病猪场的病料中，分离到３株致Ｍａｒｃ－１４５细胞病变的病毒：ＳＨ１、ＳＨ２和ＳＨ３，理化特性研究表明，３株分离毒均对氯仿、乙醚、甲醛、热（５６℃）、酸（ｐＨ６以下）、碱（ｐＨ８以上）敏感。负染可见病毒以大小不等的具囊膜的球形粒子为主，囊膜上有纤突，直径为６０￣１００ｎｍ；超薄切片可见病毒粒子分散在细胞浆内，直径为４５￣８０ｎｍ。间接免疫荧光试验表明，３株分离毒均与ＰＲＲＳＶ高免血清呈强阳性反应，而与ＨＣＶ、ＰＰＶ、ＰＲＶ、ＴＧＥＶ高免血清的反应均呈阴性。综上可见，所分离的病毒为ＰＲＲＳＶ。  相似文献   

The effect of glucocorticoid receptor on level of inflammatory media in rats after critical scald     

LIN Hai  XIAO Su-fang  LIN Zhong-qing  LIN Xue-yi  SONG Yu-na  JIANG Xi-lin 《园艺学报》2000,16(1):60-62

AIM:To further elaborate the effect of glucocorticoid receptor(GR) decrease on level of inflammatory media in rats after critical scald.METHOD:The changes of phospholipase A₂(PLA₂) activity and concentrations of tumor necrosis factoralpha (TNFα) and malondialdehyde (MDA), the product of lipid peroxidation metabolism in plasma and tissue homogenate have been studied in scalded rats with or without GR blockade by RU38486.RESULTS:The PLA₂ activity and the concentrations of TNFα and MDA in plasma and homogenate of pulmonary and renal tissue in scalded rats were significantly higher than those in the controls(P＜0.01) and further increased in scalded rats accompanied with GR blockade (P＜0.01 or P＜0.05).CONCLUSION: Blockade of GR might aggravate the increase of inflammatory mediator caused by scalding. It suggested that GR might potentiate anti-inflammation in scald.  相似文献   

3种来源的野生斑节对虾携带病毒情况调查          下载免费PDF全文

杨丽诗  黄建华  孙苗苗  周发林  杨其彬  温为庚 《上海海洋大学学报》2011,20(4):546-552

利用PCR方法,对2008年取自3个斑节对虾主产地（中国、泰国、非洲）的野生斑节对虾亲虾进行主要病毒病筛查。在所检测的210例野生亲虾样本中,均发现携带白斑综合征病毒（WSSV）、传染性皮下及造血组织坏死病毒（IHHNV）或斑节对虾杆状病毒（MBV）的阳性样本,其中泰国来源的斑节对虾亲虾WSSV携带率最高,达到89.8%,而中国来源的斑节对虾亲虾IHHNV携带率最高,为8.8%。12例样本同时检测出WSSV和IHHNV（阳性率为5.7%）。该结果揭示3种来源的斑节对虾亲虾普遍携带WSSV和IHHNV,其中WSSV仍然是感染斑节对虾的主要病原。调查结果增进了对斑节对虾主产区野生亲虾健康状况的了解,并在一定程度上揭示了WSSV感染的新规律。  相似文献   

猪繁殖与呼吸综合征病毒的研究进展   总被引：2，自引：0，他引：2  

黄园媛  李能章  彭远义 《安徽农业科学》2012,40(11):6516-6518

从遗传变异、检测以及宿主免疫3个方面,对近年来在猪繁殖与呼吸综合征病毒(PRRSV)方面的研究进行简要综述。  相似文献   

证素辨证与数字中医药     

黄惠勇 《勤云标准版测试》2012,32(11):3-6

辨证论治是中医学的主要特色,但是由于传统的辨证论治体系中各种辨证方法很难"求同存异",对于"证本质"的研究很难"以偏概全",临床惯用辨病论治替代辨证论治等弊端,限制了中医辨证学的发展。在此背景下,朱文锋教授创造性地提出了证素辨证体系,该体系通过全面收集内、外、妇、儿科等临床常见证候,并使之规范化和数量化,明确了病位证素和病性证素两大基本辨证要素。证素辨证思想体系是发展数字中医药的基础,是沟通中医药与现代科学技术的关键理论。本文结合证素辨证体系思想,提出了构建数字中医药创新研究的基本思路和框架,对促进中医药现代化研究具有重要的指导意义。  相似文献   

母猪繁殖障碍征的病因学研究   总被引：3，自引：0，他引：3  

黄佑权  盛维垠 《中国预防兽医学报》1999,21(5):333-334

某猪场一批初产母猪发生流产、早产、死胎、死仔和木乃伊胎为主要表现的繁殖障碍征。调查发现繁殖障碍占调查胎数的 ５１７％ ,其中早产占 １０８％ ,一窝中部分死胎（仔）占 ６９６％ ,全窝死亡占 １９６％ 。在因繁殖障碍损失的胎、仔数中,死胎占６９７％ ,产后即死占 ２４９％ ,木乃伊和畸形占５４％ 。血清学检测结果显示：呼吸和繁殖障碍综合征、流行性乙型脑炎和布鲁氏菌病为阴性而细小病毒为阳性,故诊断为细小病毒感染所致。  相似文献