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日本鲟肌动球蛋白热变性和冷冻变性 总被引：3，自引：0，他引：3
研究了日本鲟肌动球蛋白经变性和冷冻后ＡＴＰａｓｅ活性、总巯基数和不饱和巯基数及蛋白质浓度的变化。结果表明,热变性后肌动球蛋白Ｃａ＾２＋－ＡＴＰａｓｅ和Ｍｇ＾２＋－ＡＴＰａｓｅ活性下降,且Ｃａ＾２＋－－ＡＴＰａｅｓ活性有明显转折。低温热变性对总巯基数影响较小,温度增高时,巯基数逐渐下降,而不饱和巯基数在３０℃之前,呈动态变化,之后又逐渐减少。热变性后,肌动球蛋白浓度也降低。冷冻变性后,肌动球蛋白ＡＴ 相似文献
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ABSTRACT: The effect of pH on thermal gelation and transglutaminase (TGase; EC2.3.2.13)-induced suwari (setting) of surimi and actomyosin pastes was investigated. A strong and elastic gel was produced from walleye pollack surimi paste at pH 7.0 in the presence of Ca2+ using a two-step heating method. In contrast, walleye pollack actomyosin paste formed a weak gel under the same conditions as a result of the low concentration of endogenous TGase. In the presence of EGTA [ethyleneglycol bis(2-aminoethylether) tetraacetic acid], weak gels were formed at pH values of 7.0 and 6.0. Non-proteolytic modori (gel weakening) occurred extensively in the course of actomyosin gelation, but not in surimi gelation. Maximum TGase-induced myosin heavy chain cross-linking was observed at a slightly higher pH of 7.5 than at the optimal pH of endogenous TGase activity; the difference being derived from different substrates. Gelation of carp actomyosin paste at pH values of 5.5, 6.0, 6.5 and 7.0 was monitored by measuring storage modulus (G') and loss modulus (G"). A weak gel was formed at all pH values, but a slightly rigid and less elastic gel was obtained at lower pH values. The addition of microbial TGase (MTGase) formed strong elastic gels at pH 7.0 and 6.5. MTGase cross-linked myosin heavy chains even at pH 5.5, but contributed neither to suwari response nor strong gel formation. Overall, results suggest that the optimal pH for the gelation of surimi paste from easy-setting fish species is a compromise between the pH-optima of TGase activity and of preferable actomyosin conformation for myosin cross-linking. 相似文献
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SUMMARY: A calponin-like protein of 45 kDa was isolated from mussel anterior byssus retractor muscle (ABRM) and its inhibitory effects on actomyosin Mg2+ -ATPase was demonstrated. The 2-D electrophoresis for ABRM myofibrils gave a spot of 45 kDa protein in addition to myofibrillar proteins such as myosin and actin. The 45 kDa protein, which was more basic and showed a slightly higher molecular weight than actin, was isolated by ion-exchange chromatography and subjected to chymotryptic digestion. N-terminal amino acid sequencing of polypeptide fragments produced gave two sequences, ASQKGMTSFGAVRHH and GMDRALISKMGSKYDSGL, both of which showed a high homology to those of vertebrate calponins and invertebrate calponin-related proteins. Furthermore, the 45 kDa protein strongly reacted with commercially available antibody raised against chicken smooth muscle calponin, demonstrating that the mussel ABRM 45 kDa protein is a new member of the calponin family. Then, actomyosin Mg2+ -ATPase activity of ABRM was measured in the presence and absence of the 45 kDa protein. The 45 kDa protein clearly inhibited actomyosin Mg2+ -ATPase activity in a dose-dependent manner as in the case of other vertebrate calponins. These results indicate that the 45 kDa calponin-like protein is involved in the thin filament-associated regulation of molluscan smooth muscle contraction, possibly of a unique contraction called catch. 相似文献
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兔、猫和人的腓肠肌外侧头肌亚体与肌纤维型比较研究 总被引：2，自引：0，他引：2
目的是探讨兔、猫与人的腓肠肌外侧头肌亚体与肌纤维型的分布。采用人体解剖结合组织化学方法确定其肌亚体，并作肌动球朊ATP酶酸碱法染色，毛细血管数量统计及其三型肌纤维的直径与面积的图象分析。结果表明，兔、猫与人各肌亚体FG型纤维数比例为最高，FOG型纤维居中，SO型肌纤维最少。各肌亚体SO型纤维毛细血管数目最多，家兔、猫与人均为为内侧亚体的毛细血管／mm^2数目为最多。各肌亚体SO型纤维直径与面积为最小。说明人与哺乳动物各肌亚体均见有包裹纤维和快缩纤维的同型聚集。 相似文献
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Comparison of characteristics of actomyosin from white, pink, and red muscle fiber types in cultured carp 总被引：1，自引：0，他引：1
Qin Wang Mutsuyosi Tsuchimoto Osamu Yada Kyung H EE Abdul Jabarsyah Paula Andrea Gomez Apablaza Tosio Misima and Katsuyasu Tachibana 《Fisheries Science》2001,67(4):682-689
ABSTRACT: The present study compared and examined the characteristics of actomyosin among white (W), pink (P), and red (R) muscle fiber types in carp (cultured). Both the superprecipitation reaction and the Mg2+ -ATPase activity of actomyosin became higher with increased Ca2+ concentration (pCa 7.0–pCa 5.0) and with decreased adenosine triphosphate (ATP) concentration (3.0–0.5 mM) in all three muscle fiber types. A comparison of the three fiber types shows that the superprecipitation reaction of actomysoin was lower in the order of W < P < R and, in contrast, was higher for Mg2+ -ATPase activity in the order of W > P > R. A significantly positive correlation between both values was found for each of the three muscle fiber types, but these correlations were clearly different among the three muscle fiber types, and the superprecipitation reaction of actomyosin was lower in the order of W < P < R when Mg2+ -ATPase activity was at the same level. In conclusion, the characteristics of actomyosin were remarkably different among white, pink, and red muscle fiber types. 相似文献
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为了解重金属离子污染对鱼体质地的影响,通过外源添加不同浓度的Zn(Ⅱ)、Cu(Ⅱ)、Pb(Ⅱ)、Cr(Ⅲ)、Cd(Ⅱ)重金属离子,采用质构仪测定添加不同浓度金属离子对肌动球蛋白和肌球蛋白黏性和凝胶特性的影响。结果表明,肌动球蛋白和肌球蛋白溶液的硬度、稠度、黏度、黏聚性受离子浓度影响,硬度和稠度先下降后升高,黏性和黏聚性先略有升高,而后出现下降的趋势,Zn(Ⅱ)、Cr(Ⅲ)、Cd(Ⅱ)引起的变化显著(p＜0.01)。金属离子浓度增加对鱼蛋白凝胶的硬度、黏聚性、胶黏性、咀嚼性有显著影响(p＜0.01),降低鱼蛋白食用价值。 相似文献
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