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通过牛津杯法,从250株分离自开菲尔颗粒的乳酸菌中筛选出1株有较好抑菌活性的菌株,通过对个体特征、生理生化试验、16SrDNA序列对比,确定此株菌为乳酸乳球菌,命名为L11。在排除有机酸和过氧化氢的干扰后,发酵上清液对金黄色葡萄球菌还有抑菌活性,该菌株经过胃蛋白酶、胰蛋白酶处理后,发酵液的抑菌活性没有明显变化,说明抑菌物质为非蛋白成分,属于类细菌素,它具有较好的热稳定性,对蛋白酶不敏感,且具有较广的抗菌谱。 相似文献
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The production and partial characterization of bacteriocin‐like substances (BLSs) produced by bacteria isolated from cow manure compost were investigated. Eight BLS producers, which exhibited inhibitory activity against pathogenic bacteria, were isolated from cow manure compost at different stages of the composting process. The pile temperature ranged from 9.1°C to 73.2°C. The BLSs showed thermostability, but the BLS producers were not thermostable except for the H1 producer. Thermophilic Bacillus licheniformis H1 was further characterized. The culture supernatant of B. licheniformis H1 exhibited antagonistic activity against various species of Gram‐positive bacteria such as Listeria monocytogenes ATCC19111 but not against Gram‐negative bacteria except Pseudomonas fluorescens ATCC11251. Inactivation of bacteriocin‐like activity by α‐chymotrypsin, trypsin, and papain was highly significant (P < 0.001). The BLS was found to be stable under a pH range from 3 to 9 and at temperatures up to 75°C for 60 min, but it lost activity after being autoclaved at 121°C for 15 min. The optimum production of BLS by B. licheniformis H1 was obtained at a temperature of 55°C. Sodium dodecyl sulfate – polyacrylamide electrophoresis analysis of concentrated partially purified supernatants collected after resting the bacterial cells at 55°C revealed a bacteriocin‐like protein with a molecular mass of approximately 3.5 kDa. This study is the first report of a BLS from thermophilic B. licheniformis with an animal compost origin. 相似文献
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Janira Prichula Muriel Primon-Barros Romeu C. Z. Luz Ícaro M. S. Castro Thiago G. S. Paim Maurício Tavares Rodrigo Ligabue-Braun Pedro A. d&#x;Azevedo Jeverson Frazzon Ana P. G. Frazzon Adriana Seixas Michael S. Gilmore 《Marine drugs》2021,19(6)
New ecosystems are being actively mined for new bioactive compounds. Because of the large amount of unexplored biodiversity, bacteria from marine environments are especially promising. Further, host-associated microbes are of special interest because of their low toxicity and compatibility with host health. Here, we identified and characterized biosynthetic gene clusters encoding antimicrobial compounds in host-associated enterococci recovered from fecal samples of wild marine animals remote from human-affected ecosystems. Putative biosynthetic gene clusters in the genomes of 22 Enterococcus strains of marine origin were predicted using antiSMASH5 and Bagel4 bioinformatic software. At least one gene cluster encoding a putative bioactive compound precursor was identified in each genome. Collectively, 73 putative antimicrobial compounds were identified, including 61 bacteriocins (83.56%), 10 terpenes (13.70%), and 2 (2.74%) related to putative nonribosomal peptides (NRPs). Two of the species studied, Enterococcus avium and Enterococcus mundtti, are rare causes of human disease and were found to lack any known pathogenic determinants but yet possessed bacteriocin biosynthetic genes, suggesting possible additional utility as probiotics. Wild marine animal-associated enterococci from human-remote ecosystems provide a potentially rich source for new antimicrobial compounds of therapeutic and industrial value and potential probiotic application. 相似文献
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片球菌素是一类未修饰的具有良好热稳定性的小分子蛋白质,其理化性质稳定,抑菌谱较广,能抑制多种革兰氏阳性菌,尤其对单核细胞增多症李氏杆菌的抑制作用最为显著。本文综述了近年来片球菌素在食品防腐保鲜应用方面取得的研究进展,分析了其作为天然食品防腐剂的发展前景,同时就片球菌素当前产业化开发与市场应用所面临的问题进行了深入探讨。 相似文献
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Bacteriocins and their applications for the treatment of bacterial diseases in aquaculture: a review 
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下载免费PDF全文 Tapasa Kumar Sahoo Prasant Kumar Jena Amiya Kumar Patel Sriram Seshadri 《Aquaculture Research》2016,47(4):1013-1027
Aquaculture is one of the fastest growing food producing sectors in the world. The increase in the world population requires faster growth in aquaculture for better food availability and to overcome malnutrition. But this worldwide growth of aquaculture is overwhelmed by catastrophic fish diseases and spoilage during cultivation and preservation problems caused by pathogenic bacteria. Various remedies are available for food preservation and also from the bacterial diseases in the fish production. Due to the emergence of antibiotic resistance and adverse effects, an alternative to antibiotic is the need of the hour. The proteins such as bacteriocins, i.e. ribosomally synthesized antimicrobial peptides (AMPs) and possess antagonistic against closely related and other bacteria. These proteins are produced by most lineages of bacteria which are playing key roles in recognition and possess a cognate immunity system for self‐protection as well as host protection from infections. These proteins are potent immunomodulators with broad spectrum inhibition properties which are further used as novel therapeutic agents. In this review, we have tried to summarize the bacteriocins on the basis of their classifications, structural and functional attributes, mode of actions, bacteriocins isolated from fish and gut microbiota and presence of beneficiary bacteria in the fish gut. Further, this study highlighted where further research is a prerequisite to increase our basic understanding and search for novel bacteriocins to elucidate the proteins/peptides having antimicrobial properties for disease control in aquaculture as an alternative to antibiotics. 相似文献
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Dipta DEY Tanzila Ismail EMA Partha BISWAS Sharmin AKTAR Shoeba ISLAM Urmi Rahman RINIK Mahmudul FIROZ Shahlaa Zernaz AHMED Salauddin AL AZAD Ahsab RAHMAN Sadia AFRIN Rezwan Ahmed MAHEDI Md. Nasir Uddin BADAL 《农业科学与工程前沿(英文版)》2021,8(4):603
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试验旨研究在日粮中添加不同水平乳酸链球菌素(Nisin细菌素)对肉仔鸡血液生化指标、抗氧化及免疫指标的影响。试验采用单因素完全随机试验设计,选用1日龄爱拔益加(AA)肉鸡550只,随机分为5个处理组,每个处理5个重复,每个重复22只鸡。对照组饲喂基础日粮+土霉素6 mg·kg-1,试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组分别在基础日粮中添加Nisin细菌素0.025%、0.05%、0.075%、0.1%,试验期56 d。结果表明,在肉鸡日粮中添加不同水平的细菌素Nisin,对肉仔鸡血液生化指标和抗氧化指标没有显著影响(P0.05),显著提高肉仔鸡胸腺指数和脾脏指数(P0.05),提高肉仔鸡的免疫能力。 相似文献
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王正琼 《山地农业生物学报》2013,32(4)
食品保藏过程中腐败菌及致病菌的控制一直是困扰全球的问题.为了解决这个问题,各种新方法新技术不断出现.Nisin是乳酸链球菌产生的一种对革兰氏阳性菌具有优良抗菌活性的细菌素,目前已作为食品防腐剂在世界范围大量使用.本文综述了Nisin在食品保藏上的应用及其存在问题和展望. 相似文献
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