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排序方式: 共有110条查询结果,搜索用时 46 毫秒
1.
The goldfish vasoactive intestinal polypeptide receptor: functional studies and tissue distribution 总被引:1,自引:0,他引:1
B.K.-C. Chow 《Fish physiology and biochemistry》1997,17(1-6):213-222
2.
M Fevrier 《Comparative immunology, microbiology and infectious diseases》1985,8(2):159-170
Antibody response to an antigen involves the co-operation between three types of cells: macrophages, T cells and B cells. The cognate interactions between these cells play a fundamental role in the expression of a specific antibody response, but the last is modulated by antigen-nonspecific soluble factors produced either by macrophages or by T cells. Macrophages elaborate a spectrum of molecules modulating the function of lymphoid cells; among them are IL1 and prostaglandins of the E series, which are respectively enhancer and inhibitor of the antibody response in vitro. These molecules alter T cell and B cell activities through different mechanisms involving activation or inhibition of IL2 production, or alteration of cells surface antigens. However, the cellular events following the fixation of soluble factor on its receptors are not known. 相似文献
3.
Silva RD Bueno AL Gallon CW Gomes LF Kaiser S Pavei C Ortega GG Kucharski LC Jahn MP 《Fitoterapia》2011,82(6):818-826
This study analyzed the plasma lipid profile, glucose levels and fat deposits in male rats treated with aqueous extract of gross yerba mate, commercial yerba mate or water. Yerba mate treatment did not change body weight gain and lipid profile. The consumption of gross yerba mate significantly increased blood glucose (6.6 mmol/L) as compared to the water (4.8 mmol/L) and commercial group (5.2 mmol/L) and decreased epididymal and intra-abdominal deposits (10.1 mg/g and 23.7 mg/g of weight) as compared to the water (15.4 mg/g and 36.9 mg/g of weight) and commercial group (12.5 mg/g and 28 mg/g of weight). The results suggest that gross yerba mate reduces fat more efficiently but produces a greater increase in blood glucose when compared to commercial yerba mate and water groups. 相似文献
4.
Casas S Cortés C Ríos M Rosales T Bibbins M Olmedo V Herrera-Estrella A 《浙江大学学报(农业与生命科学版)》2004,30(4):390-390
Phototropism, the induction of carotenogenesis and reproductive structures, and resetting of the circadian rhythm are controlled by blue light. Trichoderma is used as a photomorphogenetic model due to its ability to conidiate upon exposure to light. In total darkness, T. atroviride grows indefinitely as a mycelium provided that nutrients are not limiting. However, nutrient deprivation and light trigger the conidiation process. A pulse of blue light given to a radially growing colony induc… 相似文献
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AIM: To evaluate the role of concentrated decoction of Chinese herbal compound Buyanghuanwutang (BYHWT) in cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)-cAMP response element-binding protein(CREB) signaling pathway in hippocampus of rats with vascular dementia (VD). METHODS: The rats were randomly divided into sham operation group (sham-operated rats treated with normal saline), VD model group (VD rats treated with normal saline), BYHWT treatment group (VD rats treated with BYHWT) and nimodipment treating group (VD rats treated with nimodipine). The rat model of VD was build by the method of four-vessel occlusion. The rats in all 4 groups were administered with the corresponding reagents for successive 30 days. The content of cAMP was measured by radioimmunoassay. The expression of PKA catalytic subunit (PKAc) was observed by Western blotting. The changes of DNA-binding activity of CREB in rat hippocampus were detected by electrophoretic mobility shift assay. RESULTS: The content of cAMP, the expression of PKAc and the DNA-bingding activity of CREB in the hippocampus of VD rats were lower than those in the hippocampus of sham-operated rats (P<0.01). The above indexes in both nimodipine treatment group and BYHWT treatment group were definitely higher than those in VD model group (P<0.01). CONCLUSION: BYHWT increases the content of cAMP, the expression of PKAc and the DNA-binding activity of CREB in VD rat hippocampus, thus strengthening the cAMP-PKA-CREB signaling pathway. 相似文献
7.
AIM: To investigate the neuroprotective effect of erythropoietin (EPO) on cognitive dysfunction and neuronal injury in hippocampal CA1 region induced by cerebral ischemia in mice. METHODS: Male C57BL/6 green fluorescent protein-transgenic mice were randomly divided into 3 groups: sham operation group (sham), ischemia/reperfusion group (I/R) and EPO-treated group. Transient cerebral global ischemia was induced by bilateral common carotid artery occlusion (2-VO). The step-down test was used to measure the capacity of learning and memory of the animals in each group. Nissl staining was applied to examine the neuronal number in hippocampal CA1 region. The expression of phosphorylated cAMP response element-binding protein (pCREB) was determined by Western blotting. Alterations of dendritic morphology in hippocampal CA1 region were evaluated using laser scanning confocal microscopy and Neurolucida software. RESULTS: Transient cerebral ischemia caused deficits of spatial learning and memory, and delayed neuronal death and loss of dendritic spines in hippocampal CA1 region were also obvious. The EPO treatment significantly improved the cognitive function in cerebral ischemic mice, and the protein expression of pCREB was obviously increased. At the same time, neuronal death and loss of dendritic spines were reduced in hippocampal CA1 region. CONCLUSION: Erythropoietin increases the protein expression of pCREB, and reduces neuronal death and loss of dendritic spines. These processes may be responsible for erythropoietin-mediated neuroprotective effects and the improvement of cognitive function in cerebral ischemic mice. 相似文献
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试验旨在构建猪食欲肽2受体(porcine orexin 2 receptor,pOX2R)突变体的真核表达载体,探究其野生型与突变体的基础药理学活性差异。以pcDNA3.1(+)-myc/pOX2R野生型质粒为模板,设计特异性引物单点突变构建4种突变体:pcDNA3.1(+)-myc/pOX2R-P10S、pcDNA3.1(+)-myc/pOX2R-P11T、pcDNA3.1(+)-myc/pOX2R-V308I和pcDNA3.1(+)-myc/pOX2R-T401I,将pcDNA3.1(+)-myc/pOX2R野生型和4种突变体分别瞬时转染HEK293T细胞,利用双荧光素酶报告基因检测法测定pOX2R野生型及突变体的基础活性,并检测不同浓度激动剂作用下细胞内cAMP水平,随后用内源性激动剂食欲肽A (OXA)及食欲肽B (OXB)分别对野生型及突变体进行刺激。结果显示,4个突变体构建成功,pOX2R的第10、11、308和401位氨基酸分别突变为丝氨酸、苏氨酸、异亮氨酸和缬氨酸。将pOX2R的野生型及4个突变体瞬时转染HEK293T细胞后,野生型与突变体的基础活性值无显著差异(P>0.05),表明这4个位点的氨基酸突变对其受体的基础表达信号无显著影响。与野生型受体相比,突变型受体对激动剂OXB的响应无显著差异(P>0.05),而突变体P10S、P11T和T401I对激动剂OXA的响应EC50显著降低(P<0.05),其Rmax无显著差异(P>0.05)。推测第10、11和401位点的氨基酸突变可能影响了激动剂OXA与受体的结合,降低了激动剂的激动效应。本研究结果为进一步体外研究pOX2R的功能奠定了基础。 相似文献
10.
AIM: To investigate the behavior of depression in chronic alcoholism and withdrawal model of mice, and to explore the co-mechanism of alcoholism and depression. METHODS: A novel model of chronic alcoholism was constructed in this study. The animals were divided into normal control group, and alcohol 7 d, 14 d, 21 d and 28 d groups. The mice were given alcohol preference test on the 6th, 13th, 20th and 27th days. After the test, alcohol were withdrawn for 1 d, then the next day the mice were given behavior test of depression. After the test, the mice were sacrificed. The contents of 5-hydroxytryptamine (5-HT) and norepinephrine (NE) were detected by HPLC. The expression of cAMP response element-binding protein (CREB) and brain-derived neurotrophic factor (BDNF) was detected by Western blot. RESULTS: The mice showed an obvious drinking phenomenon, and the immobility time of forced swimming test and tail suspension test was significantly increased, with increasing drinking days and withdrawal times. 5-HT level in 7 d group mice only increased in frontal cortex (P < 0.05). However, compared with control group, 5-HT levels in hippocampus and cortex were decreased on the 21th and 28th days (P < 0.01). NE levels in 21 d and 28 d groups were decreased in hippocampus and frontal cortex (P < 0.05), and no significant change was observed in 7 d and 14 d groups. The protein levels of p-CREB and BDNF were significantly decreased in hippocampus and frontal cortex of 12 d and 28 d groups (P < 0.05), and no significant change was observed in 7 d group and 14 d group. CONCLUSION: The co-mechanism of alcoholism, withdrawal and depression is related to 5-HT. 5-HT-cAMP-CREB-BDNF signaling pathway may be a common mechanism for alcoholism and depression. 相似文献