Larviculture of the painted river prawn Macrobrachium carcinus in different culture systems     

《Aquacultural Engineering》2021

The objective of this study was to evaluate different hatchery systems used for the larviculture of the Macrobrachium carcinus based on survival, larval development and production of post-larvae. The experimental culture was carried out in three phases designated as Phase I (Zoea VI to VIII – Z_{VI – VIII}), Phase II (Zoea VIII to X – Z_{VIII – X}), and Phase III (Zoea X to PL – Z_{X – PL}), with densities of 30, 27.5 and 25 larvae / L, respectively. The M. carcinus larvae (Z_VI) were reared in four culture systems, two being open (Greenwater – GW and Clearwater – CW) and two being closed (Biofloc – BFT and Bio-filter – RAS), distributed in twelve 10 L plastic containers, filled with 20 ppt brackish water, equipped with constant aeration, and water circulated by air lift and heated with thermostat (∼30 °C). The GW treatment was maintained with Chlorophyceae algae in the density of 3–5 × 10⁵ cells/mL. In the CW, the water was previously filtered through a 5 μm mesh screen, sterilized with 10 ppm active chlorine and, dechlorinated with vitamin C and subjected to aeration for 24 h. The BFT received water rich in bioflocs that was matured prior to the experiment and used molasses as a source of organic carbon. In the RAS, the culture water circulated through an external “Dry-Wet” biological filter. The feeding was carried out ad libitum four times daily, alternating a wet diet formula with a commercial diet, which was supplemented with newly hatched Artemia nauplii at a rate of 40–50 per larvae/day. Temperature, dissolved oxygen and pH were monitored daily and the salinity two times per week. Total ammonia, nitrite, nitrate, orthophosphate, alkalinity, total suspended solids, chlorophyll-a, COD and BOD were also analyzed. The best water quality (P < 0.05) was obtained in the RAS, with 0.49 (±0.38), 0.23 (±0.22), and 9.0 (±1.5) mg/L of TAN, NO₂-N and NO₃-N, respectively. In the GW, the nitrogen species showed high fluctuations and higher concentrations at 2.32 (±1.68), 3.53 (±3.53) and 18.2 (±12.9) mg / L of TAN, NO₂-N and NO₃-N, respectively. Considering the three phases (Z_{VI – PL}), the overall survival was 0.03, 1.97, 2.23 and 17.32 % for the BFT, CW, GW and RAS, respectively. When considering the phases separately, the survival in Phase I (Z_{VI – VIII}) was highest in the GW system at 58.7 % while the RAS was the highest in Phases II (Z_{VIII – X}) and III (Z_{X – PL}) at 70.6 % and 60.3 %, respectively. The BFT showed 8.4 (±3.5) PL/L, which was higher (P < 0.05) than that obtained in the RAS (2.8 ± 1.2 PL/L) and the GW (1.3 ± 1.1 PL/L) and similar to that obtained in the CW (5.6 ± 2.0 PL/L). Thus, the larviculture for the M. carcinus may be optimized by adopting a multiphase management strategy, which the intermediate larval stages (Z_{VI – IX}) are reared in the GW system and the final stages (Z_{X – PL}) are reared in the BFT system.  相似文献   

黑龙江流域中俄保护区现状及展望   总被引：1，自引：0，他引：1  

汪凌峰  史蓉红  张明海 《野生动物》2006,27(6):11-13

黑龙江地处中俄边界，在流域内中俄积极开展保护合作。本文分析了中俄在黑龙江两侧的保护区类型、保护对象，结果表明黑龙江流域的众保护区具有共同的保护对象，保护区的类型也惊人的相似。这些相同相似之处对于中俄保护合作深入发展有重要义。  相似文献   

沙土区薄壁连拱护岸墙试验与应用     

张玉建  沈波 《排灌机械》2005,23(6):46-49

沙土区薄壁连拱护岸墙为悬臂式支撑、薄壁连拱无底板结构，拱板中间采用无砂砼排水孔的钢筋砼肋形预制板，冲沉法施工，造价低廉；外形美观，抗倾、抗滑性能强。该墙为平原沙土区河道水土保持及修复找到了一条经济合理、施工方便、简便易行的护坡办法，具有较高的实用价值和广阔的推广使用前景。  相似文献   

护坦与丁坝在林区沿河公路防护中的应用     

林炳淦 《福建林业科技》2005,32(4):167-169

研究分析了丁坝和护坦在沿河林区公路路基防护中的作用、防护原理、设置方法、应用条件和有效性问题。  相似文献   

南美白对虾淡水养殖试验报告   总被引：3，自引：0，他引：3  

陈德福  孟凡峰等 《齐鲁渔业》2002,19(4):15-16

采取人工调兑淡水盐度，定点投喂颗粒饲料，调节水质及病害预防措施，在667m^2淡水池塘中养殖南美白对虾，经107天的饲养管理，共产南美白对虾160．2kg，平均体长达13．5cm/尾，体重20g/尾，成活率达到80．1％，共获利润5732．5元，投入产出比1：2．47。  相似文献   

从土地覆盖变化看塔里木河中下游天然草地的退化     

徐海量  宋郁东  陈亚宁 《中国草地学报》2003,25(4):21-24

通过GIS技术和1990～2000年的TM影像图,分析了塔里木河中下游近10年的土地覆盖动态变化,结果显示:在塔里木河中下游地区,天然草地退化严重,高、中覆盖草地大幅减少,低覆盖草地的数量虽然增加但主要是流域河岸胡杨林群落退化后向荒漠灌丛草地演化的结果;而在自然环境更加恶劣的塔里木河下游地区,随着断流和地下水位的下降,天然草地正在向裸地和沙地演变。通过分析,提出了恢复塔里木河中下游天然草地的治理建议。  相似文献   

Expression of a recombinant crustacean hyperglycemic hormone of the kuruma prawn Penaeus japonicus in methylotrophic yeast Pichia pastoris     

TSUYOSHI  OHIRA  HIDEKAZU  KATAYAMA  KATSUMI  AIDA  HIROMICHI  NAGASAWA 《Fisheries Science》2003,69(1):95-100

ABSTRACT:   Until now, six crustacean hyperglycemic hormones (CHH) designated Pej-SGP-I, -II, -III, -V, -VI and -VII have been characterized in the kuruma prawn Penaeus japonicus . All CHH consist of 72 amino acid residues and have an amidated carboxyl (C)-terminus. In the present study, we expressed Pej-SGP-III in methylotrophic yeast Pichia pastoris in order to obtain a large quantity of recombinant CHH possessing biological activity. A cDNA encoding Pej-SGP-III that had been previously cloned was processed by polymerase chain reaction (PCR) and the resulting product was ligated into an expression vector. Pichia pastoris was transformed with this vector after which a recombinant Pej-SGP-III was expressed having an additional amino acid residue (glycine) at the C-terminus (rPej-SGP-III-Gly), a form considered to be a putative precursor of this hormone. rPej-SGP-III-Gly secreted into the culture medium was purified by reversed-phase HPLC, and amidated using a peptidylglycine alpha-amidating enzyme. The amidated rPej-SGP-III (rPej-SGP-III) showed hyperglycemic activity in in vivo bioassay almost comparable to that of the natural Pej-SGP-III. rPej-SGP-III thus obtained will be a useful tool not only for its physiological study but also for the determination of its 3-D structure.  相似文献   

Trends in NE Atlantic landings (southern Portugal): identifying the relative importance of fisheries and environmental variables   总被引：7，自引：1，他引：6  

KARIM ERZINI 《Fisheries Oceanography》2005,14(3):195-209

Time series of commercial landings from the Algarve (southern Portugal) from 1982 to 1999 were analyzed using min/max autocorrelation factor analysis (MAFA) and dynamic factor analysis (DFA). These techniques were used to identify trends and explore the relationships between the response variables (annual landings of 12 species) and explanatory variables [sea surface temperature, rainfall, an upwelling index, Guadiana river (south‐east Portugal) flow, the North Atlantic oscillation, the number of licensed fishing vessels and the number of commercial fishermen]. Landings were more highly correlated with non‐lagged environmental variables and in particular with Guadiana river flow. Both techniques gave coherent results, with the most important trend being a steady decline over time. A DFA model with two explanatory variables (Guadiana river flow and number of fishermen) and three common trends (smoothing functions over time) gave good fits to 10 of the 12 species. Results of other models indicated that river flow is the more important explanatory variable in this model. Changes in the mean flow and discharge regime of the Guadiana river resulting from the construction of the Alqueva dam, completed in 2002, are therefore likely to have a significant and deleterious impact on Algarve fisheries landings.  相似文献   

Food resources and cyprinid diet in permanent and temporary Mediterranean rivers with natural and regulated flow          下载免费PDF全文

Carlos M. Alexandre  Sabrina Sales  Maria Teresa Ferreira  Pedro R. Almeida 《Ecology of Freshwater Fish》2015,24(4):629-645

This study addresses the differences in food availability, diet and feeding activity of the Iberian barbel, between permanent and temporary nonregulated rivers, and the effect of flow regulation on feeding parameters. A total of 267 adult barbels were seasonally collected in four nonregulated and regulated rivers from permanent and temporary basins, and their gut content was analysed. Locally available food sources were evaluated across sites and seasons. Barbels from the permanent nonregulated river exhibit a more variable and diversified diet in which invertebrates assumed a large importance, especially during high flows. Barbels from the temporary nonregulated river presented a more uniform diet composed of plant material and detritus, particularly in drought seasons. Flow regulation affected different flow components in both systems, but the effects on food resources and barbels' diet were similar, resulting in an intra‐annual stabilisation of resource availability and fish diet, with a higher consumption of plants and detritus. Changes in fish diet and feeding activity in both nonregulated and regulated rivers were strongly associated with the seasonal variability of streamflow components, particularly between low‐ and high‐flow periods, and with the reduction in flow variability in the case of dam regulation. Results from this study can be used to improve guidelines for flow requirement implementation.  相似文献   

Polymorphic mountain whitefish (Prosopium williamsoni) in a coastal riverscape: size class assemblages,distribution, and habitat associations          下载免费PDF全文

James C. Starr  Christian E. Torgersen 《Ecology of Freshwater Fish》2015,24(4):505-518

We compared the assemblage structure, spatial distributions, and habitat associations of mountain whitefish (Prosopium williamsoni) morphotypes and size classes. We hypothesised that morphotypes would have different spatial distributions and would be associated with different habitat features based on feeding behaviour and diet. Spatially continuous sampling was conducted over a broad extent (29 km) in the Calawah River, WA (USA). Whitefish were enumerated via snorkelling in three size classes: small (10–29 cm), medium (30–49 cm), and large (≥50 cm). We identified morphotypes based on head and snout morphology: a pinocchio form that had an elongated snout and a normal form with a blunted snout. Large size classes of both morphotypes were distributed downstream of small and medium size classes, and normal whitefish were distributed downstream of pinocchio whitefish. Ordination of whitefish assemblages with nonmetric multidimensional scaling revealed that normal whitefish size classes were associated with higher gradient and depth, whereas pinocchio whitefish size classes were positively associated with pool area, distance upstream, and depth. Reach‐scale generalised additive models indicated that normal whitefish relative density was associated with larger substrate size in downstream reaches (R² = 0.64), and pinocchio whitefish were associated with greater stream depth in the reaches farther upstream (R² = 0.87). These results suggest broad‐scale spatial segregation (1–10 km), particularly between larger and more phenotypically extreme individuals. These results provide the first perspective on spatial distributions and habitat relationships of polymorphic mountain whitefish.  相似文献