原发性纵隔肿瘤及囊肿71例分析(附1例罕见胸腺瘤并发症)     

胡新荣  邹世海  黄书伟  董瀚基 《湛江医学院学报》1992,(3)

71例原发于纵隔的肿瘤及囊肿,其中胸腺瘤占35.2％,畸胎瘤占23.9％,神经源性肿瘤占18.2％,其他肿瘤占22.7％。三类常见肿瘤中,以胸腺瘤恶性率最高(32％),神经源性肿瘤次之(15.4％),畸胎瘤无1例恶性。术前正确诊断25例,未能分类25例,误诊21例。本文分析了误诊原因。此外,报道1例罕见的胸腺瘤并发症。  相似文献   

Altered vitamin D metabolic system in follicular cysts of sows     

Malgorzata Grzesiak  Magdalena Socha  Anna Hrabia 《Reproduction in domestic animals》2021,56(1):193-196

This study aimed to examine 25OHD₃ concentration in the fluid of follicular and follicular lutein cysts of sows in comparison with preovulatory follicles as well as immunolocalize vitamin D metabolic enzymes (CYP27B1 and CYP24A1) and determine their protein abundances in the cyst wall. We have shown for the first time that 25OHD₃ level in the fluid of both cyst types was significantly lower than in preovulatory follicles. Furthermore, we have demonstrated CYP27B1 and CYP24A1 protein immunolocalization and abundance in follicular and follicular lutein cysts. The abundance of protein for both metabolic enzymes was decreased in ovarian cysts when compared to preovulatory follicles. We propose that altered VD metabolism in ovarian cyst might associate with their formation in sows.  相似文献   

Possible applications of modern fish larviculture technology to ornamental fish production     

P. Dhert  L.C. Lim  P. Candreva  H. Van Duffel  P. Sorgeloos 《Aquarium Sciences and Conservation》1997,1(2):119-128

There has been rapid development in the marine foodfishlarviculture technology in Europe since the early eighties,especially in the flat fish, turbot and halibut, and the bass andbream species. The most significant improvements in the eightieswere the introduction of light control, artificial reproductiontechniques, appropriate water treatment and the use of rotifersand Artemia nauplii of specific sizes and in the late eightiesand early nineties the quality enhancement of live food organismsusing specific enrichment techniques. Present research is focusedon the implementation of several microbial techniques to improvethe hygiene of live prey and fish.Many of the modern larviculture techniques being used in marine foodfish could be adapted for application in the ornamental fish industry. For examples, research in the Onamental Fish Section, Primary Production Department, Singapore has demonstrated that the use of freshwater rotifers and Artemia nauplii would enable artificial rearing of Discus in the absence of the parent fish and improve the larval performance of Gouramis and Tetra larvae. The use of such small live food organisms is likely to facilitate breeding of new fish species with small larvae. Research has also indicated that the use of diets containing vitamin C and certain immuno-stimulants improved the stress resistance of guppy. Such techniques would have important application in the fish transport, an important aspect in the ornamental fish industry  相似文献   

Guanine-cytosine contents of the host and symbiont cDNA in a symbiotic coral     

Shin -Ichi Kii  Jiro Tanaka  Toshiki Watanabe 《Fisheries Science》2007,73(6):1362-1372

ABSTRACT:   Hermatypic (reef-building) corals harbor dinoflagellate endo-symbionts Symbiodinium spp. In studying gene expression in such symbiotic corals, problems arise regarding how to distinguish the coral and symbiont mRNA, and how to estimate their fractions in the mRNA population of the holobiont (symbiotic complex of the coral and Symbiodinium cells). In this study, these issues were addressed using juveniles of hermatypic coral Acropora tenuis in symbiosis with Symbiodinium cells of strain PL-TS-1. First, the guanine-cytosine (GC) contents were determined in expressed sequence tags (EST) from PL-TS-1 cells cultured in vitro and symbiont-free larvae of A. tenuis , and their average GC contents were found to be significantly different. The average GC content of the EST from the holobiont was much closer to that of A. tenuis larvae, suggesting that the majority (>90%) of mRNA isolated from the holobiont originated in the host. In protein-coding sequences, little overlap was observed between the GC-content distributions of PL-TS-1 cells and A. tenuis larvae. All of the coding sequences ( n  = 59) found in the A. tenuis EST had GC contents below 0.5, whereas the GC content exceeded 0.5 in the majority (43/44) of coding sequences from the nuclear genome of PL-TS-1 cells.  相似文献   

Decapsulated Artemia Cysts: A Suitable Dietary Supplement for Juvenile Tench (Tinca tinca L.)     

Vanesa García  José M. Carral  María Sáez-Royuela  Rocío González  Álvaro González 《Journal Of Applied Aquaculture》2013,25(1):57-65

There are no specifically formulated dry foods for tench (Tinca tinca L.), which forces farmers to use diets formulated for other fish species. This has major drawbacks, such as high mortality, slow growth, and body deformities. A 120 day experiment was performed with five-month-old juvenile tench (initial mean weight: 0.388 g; total length: 31.78 mm) to evaluate decapsulated Artemia cysts as a supplement to a dry diet for other fish species. Three treatments, differing in the daily supplement, were tested: 1,800 freshly hatched nauplii, 1,800 cysts, and 300 cysts per g of tench biomass. Final survival ranged between 95.3% and 97.9%. Juvenile tench that received the supplement of 1,800 decapsulated Artemia cysts had a specific growth rate (1.28), weight (1.83 g), and total length (52.30 mm) significantly higher than those with the same amount of nauplii. The lowest supplement (300 cysts/g of fish biomass) allowed significantly lower growth and higher condition coefficient (1.40) than the rest. Animals with body deformities (1.06%) were only recorded in the groups that received the lowest cyst supplement. Results showed Artemia cysts are a suitable dietary supplement for juvenile tench, being an advantageous alternative to live nauplii.  相似文献   

Development and application of fluorescence in situ hybridization (FISH) method for simple and rapid identification of the toxic dinoflagellates Alexandrium tamarense and Alexandrium catenella in cultured and natural seawater   总被引：2，自引：0，他引：2  

Shoko Hosoi-Tanabe  Yoshihiko Sako 《Fisheries Science》2006,72(1):77-82

ABSTRACT:   The toxic dinoflagellates Alexandrium tamarense (Lebour) Balech and A. catenella (Whedon and Kofoid) Balech produce potent neurotoxins, such as saxitoxin and gonyautoxin and have been mainly responsible for paralytic shellfish poisoning (PSP) in Japan. To prevent a negative effect on the fishery industry, it is necessary to identify these toxic species precisely and rapidly before and during the bloom. In this paper, a rapid and simple protocol of a fluorescence in situ hybridization (FISH) method using ribosomal RNA (rRNA)-targeted probes has been established for identifying the cultured strains and natural cells of A. tamarense and A. catenella . Using the FISH method established in this study, it was possible to identify these toxic species species-specifically and rapidly, within 30 min. The procedure of detection constituted three steps: (i) fixation/dehydration; (ii) hybridization; and (iii) washing; this made the identification simple. Moreover, this method did not require either special techniques or equipment, and the cost for detection was low. The specificity, rapidity, and simplicity of the developed method suggest that it might be useful for routine monitoring of these toxic microalgae.  相似文献   

卤虫卵与空壳的分离技术     

李革  张强  杨承忠  钱翌  范勇  喻梅辉  周立功  李新会  那思拉  姜明阳 《新疆农业大学学报》1994,(2)

本文主要对卤虫卵加工中采用淡水比重法分离虫卵和空壳的方法进行了较为详细的论述．对近几年有关这方面的试验研究及存在的一些问题进行了概括和总结．  相似文献   

Cyst of the common bile duct in a cat     

J-G Grand  M Doucet  O Albaric  S Bureau 《Australian veterinary journal》2010,88(7):268-271

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Studies on amoebae and cysts associated with the isolation of Spongospora subterranea f.sp. subterranea in vitro     

X.-S. Qu  J. A. Kavanagh†  D. Egan  H. Lahert 《Plant pathology》2001,50(4):420-426

New evidence is presented to support the contention that the amoeba/cyst colonies isolated from surface-sterilized Spongospora subterranea f.sp. subterranea -infected potato tubers and spore balls have a saprophytic phase but are contaminants and not S. subterranea. Amoebae isolated from infected tissues and spore balls formed colonies associated with bacteria on 1% water agar at 18°C and encysted after 5–7 days. These cysts were morphologically distinct from the resting spores of S. subterranea and were formed singly or in a layer, unlike the spore ball (cystosorus) of S. subterranea . Amoebae, cysts and mixtures of amoebae and cysts in primary, secondary and tertiary subcultures failed to infect tomato roots. PCR amplification of DNA from amoebae, cysts and spore balls using the S. subterranea -specific primer pair SsF/R generated a 434-bp product from S. subterranea spore balls only and not from amoebae or cysts. When an amoeba/cyst-specific primer pair AmF/R was designed and used for PCR amplification, a single 411-bp product was generated from DNA of amoebae and cysts, but not from DNA of S. subterranea spore balls. These results are discussed in relation to earlier reports claiming the successful isolation of S. subterranea and other plasmodiophorids in vitro .  相似文献   

Nodular dermatofibrosis and cystic renal disease in three mixed-breed dogs and a Boxer dog     

White  Rosychuk  Schultheiss  & Scot 《Veterinary dermatology》1998,9(2):119-126

Three mixed-breed dogs and a Boxer dog with nodular dermatofibrosis are described. The three mixed breed dogs had concurrent renal epithelial cysts ( n  = 2) or renal cystadenomas ( n  = 1); these lesions were visualized antemortem on ultrasonographic examination. One of these dogs was a 7-year-old Labrador Retriever-Gordon Setter mixed breed which survived 5 years after diagnosis, and was euthanized for unrelated causes. The Boxer dog had renal cystic adenomatous hyperplasia and renal cystadenocarcinomas which were not visualized on ultrasonographic examination but were diagnosed at necropsy.  相似文献