外源NO及反向调控PEG胁迫下苜蓿萌发种子抗氧化酶及其同工酶动态的研究     

辛夏青  魏小红  韩厅  岳凯  赵颖 《草业学报》2018,27(10):105-112

以紫花苜蓿为材料,用一氧化氮供体硝普钠(SNP)及一氧化氮清除剂c-PTIO对苜蓿种子进行浸种处理,采用分光光度法和同工酶电泳技术来研究外源NO及反向调控对PEG胁迫下紫花苜蓿抗氧化酶活性及其同工酶的影响,并探讨NO调控苜蓿种子耐旱性的生理机制。结果表明:PEG胁迫下外施0.1 mmol·L^-1 SNP,第6天时较PEG处理POD活性降低了32.72%;第4天时SOD、CAT活性较PEG处理升高了10.48%、23.60%,有效缓解了PEG对紫花苜蓿萌发中种子的氧化损伤,提高其抗氧化能力。PEG胁迫下添加 c-PTIO抑制了苜蓿萌发期的抗氧化系统活性。从同工酶的谱带数量和强弱来看,POD同工酶各区带活力均随PEG胁迫时间的延长而增加,在第2天酶带只有1条,而第4天酶带呈现9条;SOD和CAT同工酶表达量变化不显著,但酶带强弱有一定变化,S3酶带随处理时间的延长表达量逐渐减弱;CAT同工酶谱带则一直保持2条带,无明显强弱变化。因此,外源NO在PEG胁迫下紫花苜蓿萌发中抗氧化酶快速响应并在维护氧自由基代谢平衡中起重要保护作用。  相似文献   

撒坝猪血液蛋白多态性研究   总被引：1，自引：0，他引：1  

胡文平  连林生 《云南农业大学学报(自然科学版)》1995,10(3):231-235

 采用蛋白电泳技术研究撒坝猪血液蛋白多态性。共分析了撒坝猪33个遗传位点,其中AKP,CAT,ES,G₆PD,PA,PHI,TF等7个位点检测到多态性。多态位点百分比P=0.2121,平均杂合度H=0.1027.结果表明撒坝猪的血液蛋白多态程度较高,反映在蛋白质水平上的遗传多样性较为丰富。  相似文献   

毛细管电泳及其在药物分析中的应用     

刘艳华 《中国兽药杂志》2003,37(11):45-47

阐述了不同操作模式毛细管电泳的分离机制，介绍了其在药物分析中的应用。  相似文献   

Biochemical properties of the pathogenesis-related proteins from tobacco     

J. F. Antoniw  R. F. White 《European journal of plant pathology / European Foundation for Plant Pathology》1983,89(6):255-264

This review describes the discovery and identification of the pathogenesis-related proteins (PRs) from tobacco. In crude leaf extracts the PRs are distinguished from the proteins in uninfected plants by their solubility at pH 3, resistance to a range of proteases, and mobility in polyacrylamide gels upon electrophoresis (PAGE) in non-denaturing conditions. PAGE has been used as a qualitative and semi-quantitative assay for PRs, and their migration in gels made from different acrylamide concentrations has been used to identify charge and size isomers and electrophoretically identical PRs in different tobacco cultivars. The subunit composition and molecular weight (mol. wt) of the four PRs identified first in Xanthi-nc were determined by SDS-PAGE; staining the gels has shown that these same four proteins in Samsun NN did not contain carbohydrate, lipid or nucleic acid, nor were they isozymic forms of twenty five enzymes known to increase in activity following infection with TMV. Evidence suggests that most of the PRs in Xanthi-nc and Samsun NN are extracellular.The purification of several PRs from Xanthi-nc, Samsun NN and other tobaccos is described, as well as their mol. wt, subunit and amino acid composition. PRs 1a, b and c consist of a single polypeptide and have similar mol. wt and amino acid compositions. Antisera prepared against purified Xanthi-nc b₁ protein have been used to determine serological relationships between PRs and form the basis of a very sensitive quantitative assay using ELISA. The regulation of synthesis of some PRs has been shown to involve translational control.  相似文献   

应用种子蛋白电泳图谱对高羊茅品种进行鉴别与聚类研究   总被引：2，自引：2，他引：0  

范莉英  李德颖 《草业学报》1996,5(4):5-10

利用种子贮藏蛋白进行二二烷其磺酸钠－聚丙烯酰胺凝脉电泳,对２３个高羊茅品种进行了 分析。表明该方法成本低,效率高,重复性好,能有效地反应高羊茅品种的遗传差异和亲缘关系。电泳谱带聚类分析所得到的类群,能代表生产上,饲用,草坪用类型以及两种用途中的主要生态类型。  相似文献   

Lipoprotein electrophoresis differentiation of chylous and nonchylous pleural effusions in dogs and cats and its correlation with pleural effusion triglyceride concentration     

Waddle JR  Giger U 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1990,19(3):80-85

Pleural effusions from 23 dogs and 25 cats hospitalized between November 1986 and April 1987 at the Veterinary Hospital of the University of Pennsylvania (VHUP) were classified as chylous (chylomicrons present) or nonchylous (chylomicrons absent) by the presence or absence, respectively, of a chylomicron band on lipoprotein electrophoresis gels of the effusions. Triglyceride concentrations, cholesterol concentrations, and cholesterol/triglyceride (C/T) ratios were compared between the chylous and nonchylous groups in each species. Cholesterol concentrations were not significantly different between chylous and nonchylous effusions in both dogs and cats. Cholesterol/triglyceride ratios of less than 1 were present in all dogs and cats with chylous effusions; however, 12% of dogs with nonchylous effusions and 50% of cats with nonchylous effusions also had a C/T ratio less than 1. Triglyceride concentrations accurately classified all effusions as chylous or nonchylous in both dogs and cats. Pleural effusions with triglyceride concentrations greater than l00 mg/dl were chylous in all cases, whereas, effusions with triglyceride concentrations less than l00 mg/dl were nonchylous in all cases.  相似文献   

Evaluation of a radioimmunoassay for type III procollagen peptide in the dog     

Dimski DS  Brooks CL  Johnson SE 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1990,19(2):40-44

Type III procollagen peptide (P-3-P) is a serum marker for hepatic fibrosis in humans. The utility of a commercially available radioimmunoassay for P-3-P was evaluated in the dog. The specificity of the assay was assessed by polyacrylamide gel electrophoresis (PAGE) of canine serum and purified bovine P-3-P, followed by Western immunoblotting with rabbit aniti-P-3-P serum. The sensitivity was assessed by performing the radioimmunoassay on dilutions of sera from 22 dogs. Polyacrylamide gel electrophoresis of purified bovine P-3-P and sera from two dogs suspected of having elevated P-3-P concentrations revealed no homologous bands of staining. Western immunoblotting showed marked cross-reactivity of the high antisera concentrations with several components of the serum proteins, but none corresponding to the purified P-3-P. All tested sera from dogs had minimal competitive binding with radiolabeled P-3-P in the radioimmunoassay. Dilution curves of dog sera did not parallel either the standard curve or the dilution curve of a known test human serum. There were no statistically different P-3-P concentrations in any of the groups of dogs studied. It was concluded that currently available radioimmunoassay kits for the measurement of P-3-P in the human are not applicable in the dog. Seemingly, the structure or metabolism of canine P-3-P may vary significantly from that of the bovine or human, limiting the sensitivity and specificity of this assay in the dog.  相似文献   

蛋白质组学研究技术及其在果树学中的应用   总被引：7，自引：3，他引：7  

曹尚银  张秋明  郭俊英  陈玉玲 《果树学报》2005,22(2):138-142

蛋白质组研究是当今生命科学发展的一个新的增长点,它能阐明基因组所表达的真正执行生命活动的全 部蛋白质的表达规律和生物功能。简要介绍了蛋白质组学产生的科学背景、研究方法和研究内容。蛋白质组学研究 方法主要有双向聚丙烯酰胺凝胶电泳(2D-PAGE)、质谱(Mass-spectrometric)技术、蛋白质芯片(Protein chips)技术、酵母 双杂交系统(Yeast two-hybrid system)、双向高效柱层析和生物信息学等。其应用的范围包括植物群体遗传学、在个体 水平上植物对生物和非生物环境的适应机制、植物的发育和组织器官的分化过程,以及不同亚细胞结构在生理生态 过程中的作用等诸多方面。同时展望了植物蛋白质组学研究前景以及蛋白质组学技术在果树学中的应用前景。  相似文献   

pH对洛美沙星与BSA之间结合常数的影响     

刘世旺  徐艳霞  夏小环 《安徽农业科学》2011,39(35):21797-21799

[目的]研究洛美沙星与血清白蛋白之间的作用,了解药物分子在体内的运输和分布情况。[方法]采用毛细管区带电泳法,通过测定在不同pH值、不同牛血清白蛋白浓度的缓冲溶液条件下药物迁移时间的变化,计算出洛美沙星与牛血清白蛋白相互作用的结合常数（Kb）。[结果]pH分别为6.8,7.4和8.0时,洛美沙星与BSA之间的Kb分别为10.185×1045、.319×1045、.356×104 L/mol。[结论]环境pH对洛美沙星与BSA之间的相互作用有强烈影响,当pH处在6.8～7.4之间时,Kb对pH尤为敏感。  相似文献   

高压电场干燥和热风干燥对马铃薯蛋白质二级结构的影响     

杨娇  于洪剑  白爱枝  温伟  王云龙 《保鲜与加工》2020,20(3):85-89

以自然风干为对照组,采用SDS-PAGE凝胶电泳和圆二色光谱法研究了高压电场干燥(HVEF)和热风干燥对马铃薯蛋白质分子量的影响以及两种干燥方法对马铃薯蛋白质二级结构的影响。SDS-PAGE凝胶电泳结果显示:两种干燥方法对马铃薯蛋白质分子量基本不产生影响。圆二色光谱检测结果表明:经高压电场干燥后,马铃薯蛋白质的α-螺旋所占比例下降1.30%,β-折叠所占比例上升1.60%,β-转角所占比例上升0.30%,无规卷曲所占比例上升1.20%;经热风干燥后,马铃薯蛋白质的α-螺旋所占比例上升了12.50%,β-折叠所占比例下降了10.70%,β-转角所占比例上升了4.30%,无规卷曲所占比例下降4.40%。通过比较:HVEF对马铃薯蛋白质二级结构中α-螺旋、β-折叠、无规卷曲的作用趋势与热风干燥正好相反;如,热风干燥使α-螺旋的比例显著上升,而HVEF则使α-螺旋的比例下降,并且HVEF对马铃薯蛋白质二级结构的影响比热风干燥对马铃薯蛋白质二级结构的影响较小。  相似文献