Effects of three methionine sources in diets on temporal B0AT1 and ASCT2 mRNA expression in different intestinal segments of cobia,Rachycentron canadum     

Yuanfa He  Shuyan Chi  Beiping Tan  Xiaohui Dong  Qihui Yang  Hongyu Liu  Shuang Zhang 《Journal of the World Aquaculture Society》2021,52(1):171-183

A 4‐week feeding trial was conducted using five groups of hybrid grouper (61.15 ± 0.15 g) to explore the potential effects of three methionine (Met) sources. Five isonitrogenous and isolipidic diets were formulated that included a fishmeal (FM) diet; nonsupplemented (NS) diet; or NS diets with the addition of the L‐methionine (L‐Met), DL‐methionine (DL‐Met), or coated Met (Co‐Met) to obtain the same Met level as the FM diet. Fish were randomly distributed into sea cages (30 fish per cage). Weight gain and specific growth rate in the DL‐Met group and FM group were significantly higher than those in all other groups (p < .05). In the proximal and distal intestines, of the 20 gene and time combinations (10 per gene), there were 14 combinations (70%) in which there were no significant differences in gene expression levels between the FM and DL‐Met groups (p > .05). The main reason for the same growth effect between the DL‐Met and FM groups may be attributed to the synchronized absorption at most time points after feeding, which had similar expression patterns of B⁰AT1 and ASCT2 of the proximal and distal intestines between two groups.  相似文献   

饲粮精料水平和蛋氨酸铬添加剂量对舍饲滩羊生长性能、屠宰性能、肉品质和脂肪沉积的影响     

金亚东  贾柔  周玉香  马青 《动物营养学报》2021,33(2)

本试验旨在探究饲粮精料水平和蛋氨酸铬(Cr-Met)添加剂量对舍饲滩羊生长性能、屠宰性能、肉品质和脂肪沉积的影响。采用2×3双因素试验设计,2个因素分别为饲粮精料水平和Cr-Met添加剂量,其中饲粮精料水平分别设为35%(低精料饲粮,饲粮精粗比为35∶65)和55%(高精料饲粮,饲粮精粗比为55∶45),Cr-Met添加剂量分别设为0、0.75和1.50 g/(d·只)。将60只雄性滩羊羔羊[平均体重为(21±1)kg]随机分配到6个试验组,每组10只。试验期为80 d,其中预试期15 d,正试期65 d。结果显示:1)与低精料饲粮组相比,高精料饲粮组滩羊的平均日增重、屠宰率、背膘厚度、肌内脂肪含量和后腿肉比重显著增加(P<0.05),但料重比与肌肉蒸煮损失、剪切力、pH、亮度(L*)值、红度(a*)值和黄度(b*)值则显著降低(P<0.05)。2)低精料饲粮组滩羊背膘厚度、皮下脂肪厚度、肌内脂肪含量和后腿肉比重随Cr-Met添加剂量的增加而线性降低(P<0.05),但肌肉pH则线性升高(P<0.05)。3)高精料饲粮组滩羊肋肉比重、腰肉比重以及肌肉剪切力和pH随Cr-Met添加剂量的增加而线性升高(P<0.05),而肌内脂肪含量和肌肉a*值则线性降低(P<0.05)。综合本试验测定指标,建议在滩羊养殖中选择精粗比为55∶45的高精料饲粮,Cr-Met的适宜添加剂量为1.50 g/(d·只),且不建议在精粗比为35∶65的低精料饲粮中添加Cr-Met。  相似文献   

13～18周龄笼养蛋鸭适宜蛋白质和蛋氨酸水平的研究   总被引：4，自引：1，他引：3  

赵玉华  王安 《畜牧与兽医》2002,34(9):11-14

用 32 4羽满 1 2周龄的金定鸭 ,通过饲养试验、代谢试验、屠宰试验和对试鸭产蛋期 (1 9～ 2 8周龄 )产蛋性能的观察 ,研究了在等能条件下(ME :1 1 51MJ/kg) ,不同蛋白质水平 (CP :1 3 %、 1 5 %、 1 7% )和蛋氨酸水平 (Met/CP :0 0 1 7、 0 0 2 2、 0 0 2 7)组成的 9种饲粮对 1 3～ 1 8周龄笼养蛋鸭的生长性能、养分利用、体成分沉积和产蛋期产蛋性能的影响。结果表明 :该阶段饲粮粗蛋白、蛋氨酸水平对体增重影响不显著 (P >0 0 5) ;体增重与蛋氨酸 (% )呈一定的正相关 (r=0 4574) ;CP为 1 3%、Met/CP为 0 0 2 7组采食量一直较稳定、较高 ,且饲料效率又好。代谢试验 :不同粗蛋白、蛋氨酸水平饲粮对试鸭氮存留率和能量的利用率影响显著(P <0 0 5或P<0 0 1 ) ,且它们随饲粮CP水平的升高而降低 ,随蛋氨酸水平升高而升高 ;磷的利用率也分别以CP为 1 3%的水平和Met/CP为 0 0 2 7的水平极显著地高于其他水平对磷利用率 (P <0 0 1 )。屠宰试验 :当Met/CP为 0 0 2 7时 ,体氮沉积量和体磷沉积量最佳。产蛋性能 :开产日龄和高峰日龄与 1 3～ 1 8周龄CP食入量呈弱的负相关 (r=- 0 1 83 3和r=- 0 0 984) ,与蛋氨酸食入量呈正相关 (r =0 5780和r =0 435 0 )。饲粮粗蛋白、蛋氨酸水平对 1 9～ 2 8周龄  相似文献   

Growth,Meat Yield,and Economic Responses of Broilers Provided Three- and Four-Phase Schedules Formulated to Moderate and High Nutrient Density During a Fifty-Six-Day Production Period     

《The Journal of Applied Poultry Research》2006,15(2):312-325

This study examined growth responses and meat yield of broilers provided a 3- or 4-phase feeding schedule formulated to moderate or high nutrient density. Four dietary treatments were implemented consisting of high or moderate nutrient density diets that were provided for the duration of 3 phases (1 to 17, 18 to 35, and 36 to 56 d) or 4 phases (1 to 17, 18 to 35, 36 to 46, and 47 to 56 d).Provision of a 3-phase schedule did not alter growth performance, meat yield, or diet cost per final BW. Broilers receiving high nutrient diets had improved growth rate and feed conversion from 1 to 35 d. In the 3-phase schedule, birds given the high nutrient density diets had advantages in BW gain and pectoralis minor breast weight. Market changes in meat prices impact differences in gross feeding margins between diets formulated to high or moderate nutrient density more than variation in diet cost.  相似文献   

模拟发电机组专用可控硅直流调速系统     

刘觉民  夏永祥 《湖南农业大学学报(自然科学版)》1995,22(3)

本文从分析同步发电机原动系统基本数学模型入手，提出了一种实现同步发电机原动系统仿真的专用可控硅直流调速系统及其实用装置。该控制系统采用有差调节的模拟调速器，水汽管道特性模拟、自平衡特性模拟和集成块模拟触发器，以及过流与超速保护，并可实现有差－无差调节的转换。  相似文献   

不同锌源对断奶小鼠生长及机体抗氧化能力的影响   总被引：9，自引：1，他引：9  

虞泽鹏  乐国伟  施用晖  侯丽 《畜牧与兽医》2005,37(4):1-3

选用断奶小鼠72只作为研究对象,随机分为3组(对照组,硫酸锌组和蛋氨酸锌组),每组设4个重复。测定体重、组织锌含量及相关生理生化指标,以研究硫酸锌、蛋氨酸锌2种不同锌供给形式对机体的生长效应及抗氧化能力的影响。结果表明,硫酸锌组和蛋氨酸锌组均能不同程度地提高小鼠体重,蛋氨酸锌组小鼠体重显著高于对照组与硫酸锌组(P<0.05)。饲粮中添加硫酸锌可提高小鼠肝脏和血清中的锌含量(P>0.05);蛋氨酸锌组小鼠肝脏和血清锌含量高于对照组(P<0.05)及硫酸锌组(P>0.05)。2种锌源均显著提高碱性磷酸酶(AKP)、谷胱甘肽过氧化物酶(GSHPx)活性(P<0.05),不同锌源间无明显差异(P>0.05)。添加不同的锌源不同程度地提高总抗氧化能力(TAOC)、总SOD(TSOD)、铜锌超氧化物歧化酶(CuZnSOD)活性(P<0.05),蛋氨酸锌组TAOC、TSOD显著高于硫酸锌组(P<0.05)。添加锌显著降低NO含量(P<0.05),蛋氨酸锌效果显著强于硫酸锌(P<0.05);一氧化氮合酶(NOS)活性表现出与NO相反的趋势(P>0.05)。  相似文献   

瘤胃保护性蛋氨酸稳定性的研究     

燕磊  杨维仁  杨在宾  姜淑贞  张桂国 《中国草食动物》2005,25(3):13-15

采用不同比例的动物油包被蛋氨酸制成瘤胃保护性蛋氨酸I(RPMetⅠ)、瘤胃保护性蛋氨酸Ⅱ(RPMetⅡ)和瘤胃保护性蛋氨酸III(RPMetⅢ)。通过人工唾液消化试验、体内尼龙袋试验和人工真胃液消化试验,检验DL蛋氨酸(DLMet)、商品过瘤胃蛋氨酸(BypassMet)和瘤胃保护性蛋氨酸(RPMetⅠ、RPMetⅡ和RPMetⅢ)的稳定性。试验结果表明,三种动物油包被蛋氨酸的稳定性均好于商品过瘤胃蛋氨酸和DL-蛋氨酸;不同比例的动物油包被对瘤胃保护性蛋氨酸稳定性的影响差异显著(P<0.05)。  相似文献   

A comparison of two sources of methionine supplemented at different levels on heat shock protein 70 expression and oxidative stress product of Peking ducks subjected to heat stress          下载免费PDF全文

L. Guo  R. Li  Y. F. Zhang  T. Y. Qin  Q. S. Li  X. X. Li  Z. L. Qi 《Journal of animal physiology and animal nutrition》2018,102(1):e147-e154

  相似文献   

β‐hydroxy‐β‐methyl butyrate promotes leucine metabolism and improves muscle fibre composition in growing pigs          下载免费PDF全文

Fengnan N. Li  Xiangfeng F. Kong  Kang Xu  Qiuping P. Guo  Wenlong L. Wang  Lingyu Y. Zhang 《Journal of animal physiology and animal nutrition》2018,102(5):1328-1339

The aim of this study was to investigate the effects of excess leucine (Leu) vs. its metabolites α‐ketoisocaproate (KIC) and β‐hydroxy‐β‐methyl butyrate (HMB) on Leu metabolism, muscle fibre composition and muscle growth in growing pigs. Thirty‐two pigs with a similar initial weight (9.55 ± 0.19 kg) were fed 1 of 4 diets for 45 days: basal diet, basal diet + 1.25% L‐Leu, basal diet + 1.25% KIC‐Ca, basal diet + 0.62% HMB‐Ca. Results indicated that relative to the basal diet and HMB groups, Leu and KIC groups exhibited increased Leu concentrations and decreased concentrations of isoleucine, valine and EAAs in selected muscle (p < 0.05) and had lower mRNA levels of MyHC I and higher expression of MyHC IIx/IIb (p < 0.05), and there was no significant difference between the basal and HMB‐supplemented groups. Moreover, the mRNA expression levels of AMPKα and UCP3 were higher but the myostatin mRNA levels were lower in the soleus muscle of the HMB group than those from other groups (p < 0.05). These findings demonstrated that doubling dietary Leu content exerted growth‐depressing effects in growing pigs; dietary KIC supplementation induced muscular branched‐chain amino acid imbalance and promoted muscle toward a more glycolytic phenotype; while dietary HMB supplementation promoted the generation of more oxidative muscle types and increased muscle growth specially in oxidative skeletal muscle, and these effects of HMB might be associated with the AMPKα‐Sirt1‐PGC‐1α axis and mitochondrial biogenesis.  相似文献   

过瘤胃氨基酸对泌乳早期奶牛生产性能、乳成分和血液生化指标的影响     

姜富贵  李德鹏  成海建  苏文政  张召坤  时光  宋恩亮 《中国乳业》2021,(7):25-31

为研究日粮添加过瘤胃氨基酸对泌乳早期奶牛生产性能、乳成分、血液生化指标和经济效益的影响,选择96 头体况良好、健康的泌乳早期荷斯坦牛,泌乳天数为（65±15）天。采用单因素随机区组试验设计,按照年龄、胎次和产奶量相近的原则,将96 头奶牛随机分为对照组和试验组,每组48 头。对照组为基础日粮,试验组在对照组日粮的基础上添加过瘤胃蛋氨酸（RPMet）和过瘤胃赖氨酸（RPLys）,添加量分别为18 g/头·天和35 g/头·天。结果表明,与对照组相比,添加RPMet和RPLys显著提高产奶量（P=0.034）,对干物质采食量和饲料转化率无显著影响（P>0.050）;添加RPMet和RPLys显著降低乳尿素氮含量（P=0.015）,对乳脂率有增加的趋势（P=0.096）,对乳蛋白率、乳体细胞数、乳糖率和总固形物含量无显著影响（P>0.050）;添加RPMet和RPLys显著提高血清总蛋白（TP）、白蛋白（ALB）和血糖（GLU）含量,显著降低尿素氮（BUN）含量（P=0.049）,对谷丙转氨酶（ALT）、谷草转氨酶（AST）、球蛋白（GLOB）、碱性磷酸酶（ALP）、甘油三酯（TG）和总胆固醇（TC）含量无显著影响（P>0.050）;添加RPMet和RPLys可提高经济效益3.60 元/头·天。综上所述,日粮添加RPMet和RPLys可提高泌乳早期奶牛的产奶量,增加经济效益。  相似文献