中国栽培平菇的酯酶同工酶分析   总被引：14，自引：0，他引：14  

郑素月  张金霞  黄晨阳 《食用菌学报》2003,10(4):1-6

采用电泳技术对我国大规模栽培的64个平菇商业菌株进行酯酶同工酶分析。结果表明，我国栽培平菇菌种多样性丰富，64个菌株有44个酶谱类型，不同菌株有各自特有的酯酶同工酶酶谱。聚类分析表明，供试菌株在60％相似水平上分为6大类：第一类包括以白黄侧耳为代表的21个菌株；第二类包括以德国引进菌株为代表的6个未定种名菌株；第三类包括7个白黄侧耳与佛罗里达侧耳杂交的杂交种及4个未定种名菌株；第四类包括以佛罗里达侧耳为代表的5个菌株；第五类包括以糙皮侧耳为代表的14个菌株；第六类包括以肺形侧耳为代表的7个菌株。  相似文献   

Evaluating Sediment Accretion on a Relic Oyster Reef in Mobile Bay,Alabama     

《Journal Of Applied Aquaculture》2013,25(3):41-49

Abstract

Sediment traps were deployed at Fish River Reef (FRR), a relic oyster reef on the eastern side of Mobile Bay, and at Cedar Point Reef (CPR), a productive oyster reef on the western side of Mobile Bay, Alabama, from July through October 1999. Sediment accretion at FRR was two to three times greater than sediment accretion at CPR. The proportions of particulate organic matter and ash in the sediment at the two sites were similar. The proportion of coarse to fine sediment at CPR was greater than at FRR. Also, the sediment at FRR was black and had a sulfide odor suggesting anoxia, while the sediment at CPR was brown and odorless. Various fishes and invertebrates colonized the sediment traps at CPR, while those at FRR were devoid of live organisms. Oyster spat had settled on the shells in the traps at CPR but not on those at FRR. Results suggest that in order to restore oysters at FRR, cultch material should be dispersed during periods of peak larval settlement to prevent inhibitory levels of siltation on cultch before spat settle.  相似文献   

Inhibitory effect of niboshi on food intake     

Shigeru Nakajima  Minoru Hamada  Takahide Tsuchiya and  Hiromichi Okuda 《Fisheries Science》2000,66(4):795-797

  相似文献   

Evaluation of three phytoplankton species as food for the pearl oyster <Emphasis Type="Italic">Pinctada fucata</Emphasis>     

Tomoki Hashimoto  Katsuya Hyodoh  Takuma Hirose  Satoshi Nishikawa  Toshiya Katano  Shin-ichi Nakano 《Aquaculture International》2008,16(4):309-318

In the pearl cultivation farms of the Ehime Prefecture, Japan, mass mortalities of the pearl oyster Pinctada fucata have occurred since 1994. The occurrences of mass mortality roughly coincided with a shift of the dominant phytoplankton from Skeletonema and Chaetoceros to Chaetoceros and Nitzschia all of which belong to Bacillariophyceae. Hence, we evaluated Nitzschia, together with Chaetoceros and Isocrysis, as food for the oyster. Wet weights, lengths, widths, glycogen contents, and growth rates in terms of wet weight of the oysters in all the feeding treatments were significantly higher than those in the non-feeding treatment. The highest glycogen content (2.34%) and growth rate (2.21 g month⁻¹) were found in the Chaetoceros treatment. Growth rate in the Isocrysis treatment (1.63 g month⁻¹) was also high, although glycogen content in this treatment (0.41%) was low. In the Nitzschia treatment, growth rate of the oyster (0.94 g month⁻¹) was the lowest and glycogen content (0.83%) was also low relative to that in the Chaetoceros treatment. Chlorophyll a concentration in fecal pellets was lowest in the Nitzschia treatment (<2.7 μg mg⁻¹), suggesting more complete digestion of Nitzschia by the oyster. Thus, Nitzschia was edible and digestible but not assimilated by P. fucata. We propose the following scenario for the relationship between Nitzschia dominance and mass mortality. When Nitzschia dominates in a culture area, the physiological condition of P. fucata deteriorates due to low assimilation of Nitzschia by the oyster, followed by susceptibility of the oyster to infection by agents lethal to the oyster.  相似文献   

Nutritional value of Pavlova spp. (Prymnesiophyceae) for spat of the Cortez oyster Crassostrea corteziensis during late-nursery culturing at the hatchery     

Josafat J Ojeda-Ramírez  Jorge Iván Cáceres-Puig  José M Mazón-Suástegui  & Pedro E Saucedo 《Aquaculture Research》2008,39(1):18-23

Three Pavlova species were evaluated for their nutritional value as diets for growth and survival of the Cortez oyster Crassostrea corteziensis spat during late‐nursery cultivation at a hatchery. Microalgae were provided as monospecific diets (Pavlova salina, P. sp. C50 and P. sp. C53) and in binary combinations of diets 1+2, 1+3 and 2+3 at 80–90 × 10³ cells mL^?1 for 21 days. Juveniles experienced high survival rates and grew well with all dietary treatments, but binary diets yielded greater survival and growth of spat. From the three binary treatments, Diet 6 (P. sp. C50 and P. sp. C53) promoted significantly (P<0.001) fastest growth of juveniles in shell height (0.19 mm day^?1), shell length (0.14 mm day^?1), total wet weight (0.04 g day^?1) and dry weight of meat biomass (0.024 g day^?1). For all shell dimensions, the lowest growth rates occurred with Diets 2 (P. sp. C56 alone) and 3 (P. sp. C50 alone). These results highlight the importance of testing microalgal diets for bivalve spat rather than just relying on published nutritional values.  相似文献   

Application of sperm cryopreservation in selective breeding of the Pacific oyster,Crassostrea gigas (Thunberg)     

Serean L Adams  John F Smith  Rodney D Roberts  Achim R Janke  Nick G King  Harry Robin Tervit  Stephen C Webb 《Aquaculture Research》2008,39(13):1434-1442

The robustness of Pacific oyster, Crassostrea gigas (Thunberg), sperm cryopreservation in the context of selective breeding based on family lines was investigated. Irrespective of egg density, high fertilization success was achieved with cryopreserved sperm when sperm:egg ratios of 1000:1 to 10 000:1 were used. Variation among replicate runs on the same oyster batches was minimal, indicating that cryopreservation and larval rearing procedures were repeatable. Twenty independent single male–female crosses were made to assess the utility of cryopreserved sperm in selective breeding. The fertility of unfrozen sperm was generally a poor predictor of cryopreserved sperm fertility. Based on D‐larval yields, 17 of the 20 crosses were likely to yield adequate spat for selective breeding (>10⁵ D‐larvae from 1 million eggs), two were marginal (5 × 10⁴ D‐larvae) and one was inadequate (4 × 10³ D‐larvae). An alternative fertilization strategy to improve D‐yield from a given number of sperm was then tested. Fertilizing 10 million eggs at a sperm:egg ratio of 200:1 increased the total D‐yield when compared with fertilizing 1 million eggs at a sperm:egg ratio of 2000:1 for the same male–female pair. We conclude that, despite wide variation in fertility, cryopreserved sperm is useful for family production.  相似文献   

Mass preparation of marine silage from Undaria pinnatifida and its dietary effect for young pearl oysters     

Motoharu  UCHIDA  the late Katsuyuki  NUMAGUCHI  Masakazu  MURATA 《Fisheries Science》2004,70(3):456-462

  相似文献   

Osmo-responsive expression of oyster amino acid transporter gene and analysis of the regulatory region involved     

Haruhiko?ToyoharaEmail author  Masashi?Ikeda  Chika?Goto  Hideki?Sawada  Masatomi?Hosoi  Kazuaki?Takeuchi  Isao?Hayashi  Shintaro?Imamura  Michiaki?Yamashita 《Fisheries Science》2005,71(3):465-470

  相似文献   

太平洋牡蛎控温育苗高产技术     

张玉恒  王亮 《齐鲁渔业》1997,14(3):11-12

介绍了太平洋牡蛎亲贝的选择和促熟，获卵与孵化，幼虫发育和培育技术，以及附苗和单体牡蛎苗种生产方法。  相似文献   

Taurine transporter from the giant Pacific oyster <Emphasis Type="Italic">Crassostrea gigas</Emphasis>: function and expression in response to hyper- and hypo-osmotic stress     

Masatomi Hosoi  Chuya Shinzato  Masaya Takagi  Shoko Hosoi-Tanabe  Hideki Sawada  Eri Terasawa  Haruhiko Toyohara 《Fisheries Science》2007,73(2):385-394

ABSTRACT:   Taurine is the primary osmolyte in marine molluscs, whose cellular osmo-conforming process is vital for environmental adaptation because of a lack of osmotic homeostasis. Here, cDNA cloning and expression, and functional analyses of taurine transporter (TAUT) from the giant Pacific oyster are reported on. The deduced amino-acid sequence of oyster TAUT (oyTAUT) showed 47–51% identity to those of vertebrate TAUT, whereas identity among the vertebrates is 78–95%. Functional analysis of oyTAUT expressed in Xenopus oocytes revealed that oyTAUT has a lower affinity and specificity for taurine and a requirement for higher NaCl concentration, compared with vertebrate TAUT. Taken together with similar functional properties of TAUT from mussel, indicated by our previous study, it is possible that these functional features reflect the internal environment of the molluscs (i.e. higher taurine and NaCl concentrations). Oyster taurine transporter mRNA expression was induced by not only hyper-osmotic stress, similar to other TAUT, but also hypo-osmotic stress. It is speculated that the expression in response to hypo-osmotic stress was induced by a substantial decrease in tissue taurine content following the decrease in the internal osmolality.  相似文献