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1.
The current best practice intensive culture of larval Atlantic cod includes feeding rotifers from onset of exogenous feeding until 25–30 days after hatching. These larvae grow considerably slower and develop higher frequencies of deformities than larvae reared in semi‐extensive systems, using copepods as feed. The present study compares the micronutrient concentrations in rotifers with those of copepods, with the aim of identifying nutrients that may be limiting for normal growth and development of cod larvae. An additional criterion used is the nutrient requirements given for fish in general, by NRC (1993) , as nutrient requirements of cod remains to be determined. Rotifers were fed on four different diets, consisting of baker's yeast with cod liver oil (3.3 : 1 dry weight (DW)/v), baker's yeast with Algamac 2000TM (3.5 : 1 DW), baker's yeast with live algae Chlorella (4.1 : 1 DW), and Culture Selco 3000TM (CS). CS was a complete commercial diet for rotifers while the other diets are considered as based on raw ingredients. Compared with copepod nutrient levels, rotifers grown on yeast‐based diets supplemented with either cod liver oil, Algamac 2000 or Chlorella were apparently sufficient for covering the requirements in cod larvae for all the B‐vitamins, except thiamine. Rotifers cultured on the CS diet also had sufficient amounts of thiamine. Of the minerals, only calcium and magnesium were sufficient, using this criterion while iron was on the borderline. However, with reference to the requirements given for larger fish ( NRC 1993 ), only thiamine, vitamin A, manganese, selenium and perhaps copper, appear too low in the rotifers cultured without extra micronutrient supplementation. The other nutrients were present at levels intermediate between copepod and fish requirement levels. This study suggests that it is necessary to develop enrichment techniques to produce rotifers with sufficient amounts of all micronutrients. Such techniques will also be important tools for determining which nutrients are present at levels below the actual requirements in cod larvae.  相似文献   
2.
Rotifers and Artemia salina nauplii are the most widely used live prey for newly hatched larvae, but they do not always promote optimal survival and growth. Alternative food sources such as copepods, which bypass these inadequacies and promote adequate growth, are needed and they are viewed with considerable interest by the scientific community. The aim of the present study was to test two different diets [rotifers and A. salina nauplii (group A) and a mixture (group B) of rotifers/Tisbe spp. copepods and A. salina nauplii/copepods] during the larval rearing of the striped blenny Meiacanthus grammistes. The analysis of the survival rate, size (total length and wet weight) and metamorphosis time during the larval phase of this species showed that Tisbe spp. administration can significantly improve larval survival and growth and also reduce the metamorphosis time. The results obtained are related to the fatty acid content of the live prey used and are essential in order to improve the captive production of M. grammistes through a closed system and, in turn, to preserve natural stocks.  相似文献   
3.
Mulloway (Argyrosomus japonicus) is an emerging aquaculture species in Australia, but there is a need to improve the production technology and lower costs, including those associated with larval rearing and live feeds. Three experiments were conducted to determine appropriate weaning strategies from live feeds, rotifers (Brachionus plicatilis) and Artemia, to cheaper formulated pellet diets. Experiment 1 examined the effects of feeding Artemia at different levels [0%, 50% or 100% ration of Artemia fed from 18 days after hatching (dah); based on current hatchery protocols] and a pellet diet from two larval ages (14 or 23 dah). In addition, rotifers were supplied to larvae in all treatments for the duration of the experiment (14–29 dah), at which time all larvae were successfully weaned onto the pellet diet. No significant (P>0.05) differences existed between the growth of fish fed a 50% and 100% ration of Artemia; however, fish fed a 0% ration of Artemia had significantly (P<0.05) reduced growth. The time of pellet introduction had no significant (P>0.05) effects on the growth of larvae. Experiments 2 and 3 were designed to determine the size [total length (TL), mm] at which mulloway larvae selected Artemia equally or in preference to rotifers, and pellet (400 μm) equally or in preference to Artemia respectively. Each day, larvae were transferred from a holding tank to experimental vessels and provided with rotifers (2 mL?1), Artemia (2 mL?1) or a combination of rotifers (1 mL?1) and Artemia (1 mL?1) (Experiment 2), and Artemia (2 mL?1), a pellet diet or a combination of Artemia (1 mL?1) and a pellet diet that was broadcast every 15 min (Experiment 3). After 1 h, a sub‐sample of larvae was randomly selected from each replicate vessel (n=5) and the gut contents were examined under a light microscope. Mulloway larvae began selecting Artemia equally to rotifers at 5.2 ± 0.5 mm TL and selected pellets equally to Artemia at 10.6 ± 1.8 mm TL. Our results have led to the establishment of weaning protocols for larval mulloway, which optimize larval growth while reducing feed cost by minimizing the amount of Artemia used during production.  相似文献   
4.
鉴于长江中下游河网水流条件复杂,河流生境变化导致的浮游动物群落结构特异性,于2012年10月(平水期)和12月(枯水期)在长江下游江苏和安徽交界处的河网区布设6个采样点,进行轮虫和甲壳动物采样调查,并检测NH+4-N、NO-2-N、TN、TP、CODMn和Chl.a含量等水环境指标。结果表明,河网区共检出轮虫31种,优势种包括螺旋龟甲轮虫(Keratella cochlearis)和疣毛轮虫(Synchaeta sp.)等;枝角类14种,优势种为象鼻溞(Bosmina sp.);桡足类20种,优势种为跨立小剑水蚤(Microcyclops varicans)、中华窄腹剑水蚤(Limnoithona sinensis)和温剑水蚤(Thermocyclops sp.)。轮虫密度最高,平均为350.69个/L,其次为桡足类3.44个/L,枝角类最少,仅0.15个/L。轮虫和甲壳动物的季节变化规律不同,平水期的轮虫种类多于枯水期,枝角类和桡足类则相反,浮游动物的密度变化规律也类似。CCA分析表明,氮、磷、CODMn和Chl.a共同影响了浮游动物的群落结构与分布,枯水期轮虫密度与枝角类和桡足类密度均显著正相关(P0.01),叶绿素a与3类浮游动物密度也显著正相关(P0.05)。受长江汛后水文条件的影响,河网区浮游动物群落组成及优势种变化有其特定规律,轮虫与甲壳动物的种间关系也对其种类和密度的季节变化造成影响,而食物限制则是枯水期种群竞争的重要因素。  相似文献   
5.
Three species of purple non‐sulphur bacteria (PB), Rhodopseudomonas palustris, Rhodobacter sphaeroides and Rhodovulum sulfidophilum, grown in palm oil mill effluent (POME) were successfully used for the first time as feed for rotifers (Brachionus rotundiformis). Rp. palustris cultured in both POME and synthetic medium gave the highest rotifer density (332–395 individuals mL−1) from 3 to 5 days at 10 g L−1 salinity. Other PB cultured in synthetic medium generally support higher rotifer density than PB cultured in POME. Rb. sphaeroides had the highest biomass (1.91–3.34 g L−1) and growth rate (0.64–1.11 g day−1) in both types of culture medium. Nevertheless, only Rv. sulfidophilum grown in POME contained both eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), indicating its ability to biosynthesize them from POME nutrients. Rotifers fed Rv. sulfidophilum grown in POME had significantly higher amounts of protein, arachidonic acid, EPA and DHA than rotifers fed Rv. sulfidophilum grown in synthetic medium. The nutritional profile of lipid‐deficient PB can be improved by growing them in POME, and these enriched PB produced at an estimated cost of USD 8.71–35.35 kg−1 dry biomass, depending on species, can support rotifer production in a batch culture system.  相似文献   
6.
Copepods are the natural food items for marine fish larvae but are still difficult to be cultured on a continuous basis. Therefore, suitable storage techniques for copepod subitaneous eggs are of great interest. Cold‐stored copepod embryos still develop and retain a physiological activity during the cold storage period. As a consequence, their nutritional quality may change over the time of storage with these changes possibly affecting both eggs viability and larval fish survival and development. In this study, the clownfish, Amphiprion polymnus, was used as an experimental model to compare, for the first time, the effects of diets based on enriched rotifers and Artemia (control), Acartia tonsa copepods of a continuous culture and A. tonsa originated from 6 months cold‐stored eggs. The effects of the different diets were tested through morphometric, molecular and biochemical approach. This study demonstrated that after a 6 months cold storage period, the quality of copepods obtained from those eggs was suboptimal for A. polymnus larval rearing. In fact, larvae fed those copepods showed lower growth and survival performances respect to the other experimental groups.  相似文献   
7.
Rotifers are a group of soil animals which are not commonly quantified. The most recent data originated from soil studies focussed on nematodes and various modifications of the Baermann funnel, which is not suitable for rotifers. The present study developed a method for quantitative extraction of rotifers, which gives representative results describing rotifer populations and facilitates subsequent identification to the species level. The LC extractor recovered rotifer numbers that were two orders of magnitude higher than what was extracted from the Baermann funnel. The method involves spreading a soil sample on a plastic sieve and uses a very thin layer of cellulose submerged in distilled water in a Petri dish. The Petri dish is situated on a cooled panel and light from the top by fluorescent lamps. The temperature of the base panel which gave the best extraction results was determined experimentally to be 5 °C. Rotifers were not able to withstand any heating from the top, which drastically reduced extraction results.  相似文献   
8.
The ability for food selectivity of rotifer Brachionus plicatiliswas studied in the laboratory by feeding mixtures of microalgae with various cell volumes. Chlorella sp. (≈22 µm3) was the reference algal species, and Asteromonas gracilis, (Chlorophyta) (≈2150 µm3), Tetraselmis suesica (≈268 µm3), Dunaliella salina (≈52 µm3) and Chaetoceros sp. (≈150 µm3) the experimental species. Each was mixed with Chlorella and fed in three experiments. In the first experiment, filtration and ingestion rates of rotifers each fed with algae revealed that the highest values were measured with the mixture of Chlorella + Asteromonas, and the lowest for Chlorella + Chaetoceros. In the second and the third experiments, by using several combinations of algal densities with the mixture of Chlorella + Asteromonas, a selectivity ability of the rotifers for Asteromonas was found. A hypothesis is presented that accounts for the preference of rotifers for Asteromonas, which is suggested as a new candidate species for use in live food production of fish hatcheries.  相似文献   
9.
The aim of this study was to evaluate the effect of the addition of Navicula spp. and Brachionus plicatilis on water quality and growth of postlarvae shrimp Litopenaeus vannamei reared in a biofloc system. Four treatments were considered: a control (biofloc system – BFT); BFT with the addition of Navicula spp. (BFT‐N); BFT with the addition of Brachionus plicatilis (BFT‐B) and BFT with the addition of Navicula spp. and Brachionus plicatilis (BFT‐NB), each in triplicate. Shrimp (16.2 ± 0.03 mg) were stocked at a density of 2500 shrimp m?3 and plankton were added on days 1, 5, 10, 15, 20, 25 and 30 at a density of 5 × 104 cells mL?1 (Navicula spp.) and 30 organisms L?1 (Brachionus plicatilis). The shrimp were fed a formulated feed in four daily rations composed of 40% crude protein and 8% lipids. Significant differences between treatments were observed for final weight, yield, feed conversion ratio, specific growth rate, protein efficiency ratio and protein content of the shrimp. The combined plankton addition of Navicula spp. and B. plicatilis had better performance parameters, indicating their benefit as natural food sources for postlarvae L. vannamei in biofloc systems.  相似文献   
10.
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