马铃薯热激转录因子HsfA3基因的克隆及其耐热性功能分析     

唐锐敏  贾小云  朱文娇  印敬明  杨清 《作物学报》2021,(4):672-683

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Expression of Anti-Lipopolysaccharide Factor Isoform 3 in Chlamydomonas reinhardtii Showing High Antimicrobial Activity     

Anguo Li  Ruihao Huang  Chaogang Wang  Qunju Hu  Hui Li  Xiao Li 《Marine drugs》2021,19(5)

Antimicrobial peptides are a class of proteins with antibacterial functions. In this study, the anti-lipopolysaccharide factor isoform 3 gene (ALFPm3), encoding an antimicrobial peptide from Penaeus monodon with a super activity was expressed in Chlamydomonas reinhardtii, which would develop a microalga strain that can be used for the antimicrobial peptide production. To construct the expression cluster, namely pH2A-Pm3, the codon optimized ALFPm3 gene was fused with the ble reporter by 2A peptide and inserted into pH124 vector. The glass-bead method was performed to transform pH2A-Pm3 into C. reinhardtii CC-849. In addition to 8 μg/mL zeocin resistance selection, the C. reinhardtii transformants were further confirmed by genomic PCR and RT-PCR. Western blot analysis showed that the C. reinhardtii-derived ALFPm3 (cALFPm3) was successfully expressed in C. reinhardtii transformants and accounted for 0.35% of the total soluble protein (TSP). Furthermore, the results of antibacterial assay revealed that the cALFPm3 could significantly inhibit the growth of a variety of bacteria, including both Gram-negative bacteria and Gram-positive bacteria at a concentration of 0.77 μM. Especially, the inhibition could last longer than 24 h, which performed better than ampicillin. Hence, this study successfully developed a transgenic C. reinhardtii strain, which can produce the active ALFPm3 driven from P. monodon, providing a potential strategy to use C. reinhardtii as the cell factory to produce antimicrobial peptides.  相似文献   

Utilizing a DUALEM‐421 and inversion modelling to map baseline soil salinity along toposequences in the Hunter Valley Wine district          下载免费PDF全文

U. Stockmann  J. Huang  B. Minasny  J. Triantafilis 《Soil Use and Management》2017,33(3):413-424

In the oldest commercial wine district of Australia, the Hunter Valley, there is the threat of soil salinization because marine sediments underlie the area. To understand the risk requires information about the spatial distribution of soil properties. Electromagnetic (EM) induction instruments have been used to identify and map the spatial variation of average soil salinity to a certain depth. However, soils vary with depth dependent on soil forming factors. We collected data from a single‐frequency and multiple‐coil DUALEM‐421 along a toposequence. We inverted this data using EM4Soil software and evaluated the resultant 2‐dimensional model of true electrical conductivity (σ – mS/m) with depth against electrical conductivity of saturated soil pastes (EC_p – dS/m). Using a fitted linear regression (LR) model calibration approach and by varying the forward model (cumulative function‐CF and full solution‐FS), inversion algorithm (S1 and S2), damping factor (λ) and number of arrays, we determined a suitable electromagnetic conductivity image (EMCI), which was optimal (R² = 0.82) when using the full solution, S2, λ = 3.6 and all six coil arrays. We conducted an uncertainty analysis of the LR model used to estimate the electrical conductivity of the saturated soil‐paste extract (EC_e – dS/m). Our interpretation based on estimates of EC_e suggests the approach can identify differences in salinity, how these vary with parent material and how topography influences salt distribution. The results provide information leading to insights into how soil forming factors and agricultural practices influence salinity down a toposequence and how this can guide soil management practices.  相似文献   

NO对大鼠中脑腹侧被盖区DA神经元的调节     

孙碧英  张文慧 《动物医学进展》2003,24(4):66-68

实验观察了微量注射 NOS抑制剂 L -NAME及微量联合注射 NO的前体 L-精氨酸( L-Arg) L-NAME于大鼠中脑腹侧被盖区( VTA)对该部位多巴胺神经元的调节。发现VTA注射 L -NAME( 1 mg/ 5μL )后 ,伏隔核( Acb)多巴胺 ( DA )代谢产物—双羟苯乙酸( DOPAC)水平升高到注射前的 1 2 2 .5% ( P <0 .0 0 1 ) ,小剂量注射 L-NAME( 0 .2 mg/ 5μL)对伏隔核 DOPAC水平无明显影响 ;同样方法联合注射 L-Arg( 3 0 0 μg/ 5μL) L-NAME( 1 mg/5μL)后 ,伏隔核 DOPAC水平无明显变化。结论 :VTA微量注射 L-NAME兴奋了该部位的DA神经元 ,而 L -Arg L -NAME联合注射 ,却不能影响 DA神经元的活动 ,说明 NO可以通过L-Arg-NOS-NO途径参与 VTA多巴胺神经元的调节  相似文献   

茶多酚(TP)对家禽免疫功能的研究     

詹勇  李进昌  杨贤强 《浙江大学学报(农业与生命科学版)》1992,(4)

选用14日龄健康的海佩科内用仔鸡48羽,随机分成试验(24羽)和对照(24羽)两组.试验组在饲粮中添加0.25％的茶多酚(TP),研究TP对鸡免疫功能的作用.试验结果表明,TP对自然感染法氏囊病鸡,有明显提高(p<0.05)鸡血液中红细胞C_(3b)受体和免疫复合物的含量及保护鸡脾脏正常的免疫功能,同时也发现TP对公母鸡法氏囊的重量有不同影响.本试验表明,TP具有提高家禽免疫功能的作用.  相似文献   

菜用加工兼用栗永荆3号选育     

阮逸  李德静  叶茂宗 《浙江林业科技》2001,21(5):27-29

一般认为我国南方栗仅宜于菜用，加工极易开瓣和糊化，经过几年的筛选和加工试剂，永荆3号品种可生产B－E级标准的糖水栗子罐头。  相似文献   

大蒜根蛆的发生规律及防治技术   总被引：2，自引：0，他引：2  

郑露  李国敬  关银池  刘绪红  易行荣  黄俊斌 《湖北农业科学》2008,47(8)

对湖北省当阳市大蒜根蛆的发生规律、不同品种的抗虫性及药剂防治进行了调查试验。结果表明，大蒜根蛆在湖北1年发生2代，第1代根蛆危害时期为9月8～26日，第2代为9月25日至10月20日，第2代根蛆发生量大，危害严重；不同大蒜品种根蛆危害差异不大；药剂防治以10％地友GR处理土壤防效较好，为88.8％，叶面喷雾以2.5％肯打EC防效较好，为77.7％。  相似文献   

管道3PE防腐涂层补口技术标准及规范对比分析     

赵君  侯宇  张丰  王德增  刘玲莉  丁峰 《油气储运》2009,28(3)

通过对比国内外热收缩带(套)补口技术规范及标准,分析了各项性能指标间的差异,包括材料性能指标、安装工艺及质量检验.指出管道表面处理及底漆涂装是影响热收缩带(套)安装系统性能的重要因素,提出了工程技术规范编制建议.  相似文献   

"数字农业"及其实现技术   总被引：13，自引：2，他引：11  

于淑惠 《农业图书情报学刊》2004,15(7):5-7,17

文章介绍了“数字农业”的含义、特点以及其所涉及的技术体系，说明了它对我国农业发展具有重大意义。  相似文献   

串励直流电动机使用无触点可控硅斩波调速的研究     

施德昌 《南京农业大学学报》1989,12(1):118-128

本文提出了串励直流电动机使用新型无触点斩波调速的新方法。通过两组二段式斩波电路,主电路完全无触点化成为可能,而且扩大了调速使用范围,也证明了可应用于双机车牵引同步自动化控制。  相似文献