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排序方式: 共有262条查询结果,搜索用时 15 毫秒
1.
The effect of separate chilling (5°C for 2 and 4 days) on the shoots and roots of two maize hybrid seedlings on the electric potential, the composition of fatty acids and the ATPase activity in the microsomes of non-chilled leaves and roots (20°C) was investigated. It has been found that the low temperatures induced on the non-chilled organs gave similar changes to organs exposed to a reduced temperature. The changes consisted of a parallel depolarization of leaf and root membranes and similarly a decrease in the ATPase activity as well as changes in the unsaturation of the membranes' fatty acids. The reduction of the acid content 18:2 was particularly significant, which in the leaf microsomes correlated with a drop in the ATPase activity.
The similarity between the reactions of the chilled and non-chilled parts of the plant gives evidence of a communication' between the organs which may facilitate a coordinated reaction of the entire organism to stress. The background for these phenomena could be die action potential of die chilled parts of seedlings which, in the non-chilled parts, might produce changes in the structure of biomembranes and in the activity of enzymes. 相似文献
The similarity between the reactions of the chilled and non-chilled parts of the plant gives evidence of a communication' between the organs which may facilitate a coordinated reaction of the entire organism to stress. The background for these phenomena could be die action potential of die chilled parts of seedlings which, in the non-chilled parts, might produce changes in the structure of biomembranes and in the activity of enzymes. 相似文献
2.
J. Guan J. C. Kapteyn A. Kerkenaar M. A. De Waard 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(5):313-324
Differential accumulation of [14C]imazalil and [14C]fenarimol by germlings of wild-type and DMI-resistant isolates ofPenicillium italicum was studied at various pH values. At pH 7 and 8 the low-resistant isolate E300–3 accumulated 22% and 35%, respectively, less imazalil than the wild-type isolate W5. Imazalil accumulation at pH 5 and 6 was similar. Isolate E300–3 also accumulated less fenarimol as compared with the wild-type isolate. This difference was much more obvious than for imazalil and was observed at all pH values tested. Differences in accumulation of both imazalil and fenarimol between low (E300–3), medium (H17) and high resistant (I33) isolates were not observed. These results suggest that decreased accumulation of DMIs is responsible for a low level of resistance only and that additional mechanisms of resistance might operate in isolates with a medium and high degree of resistance. With all isolates fenarimol accumulation was energy-dependent. This was not obvious for imazalil.The wild-type and DMI-resistant isolates had a similar plasma membrane potential as determined with the probe [14C]tetraphenylphosphonium bromide ([14C]TPP+). Various test compounds, among which ATPase inhibitors, ionophoric antibiotics and calmodulin antagonists, affected the accumulation of [14C]TPP+, [14C]imazalil and [14C]fenarimol. No obvious correlation between the effects of the test compounds on accumulation levels of the fungicides and [14C]TPP+ could be observed. These results indicate that the plasma membrane potential does not mediate the efflux of DMI fungicides byP. italicum. 相似文献
3.
The effects of azadirachtin (AZA) and nucleopolyhedrovirus (NPV) on midgut enzyme activity in Spodoptera litura Fabricius (Lepidoptera: Noctuidae) (Tobacco cutworm) were evaluated. Gut enzyme activities were decreased by AZA and NPV individually and in combination. When S. litura larvae were fed a diet of castor leaves treated with AZA and NPV in bioassays, gut enzyme—acid phosphatases, alkaline phosphatases, adenosine triphosphatases, and lactate dehydrogenase—activities were decreased. There were statistically significant differences (P ? 0.05) in enzyme activities between combined and individual treatment. A synergistic effect of botanical insecticides and virus was found when combined in low doses. These effects are most pronounced in early instars. Maximum weight loss (59-72%) occurred, when AZA and NPV were combined. 相似文献
4.
Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation 下载免费PDF全文
Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane‐impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm. A low concentration of sucrose plus trehalose (S15T15) was optimal. Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15T15) gave the best results for both A. sinensis and A. dabryanus. Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting. Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis. The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15T15 in cryopreserved A. sinensis sperm. There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight‐line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm. Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis. 相似文献
5.
蛇床子素可抑制小麦赤霉病菌菌丝生长干重;含有14C标记的葡萄糖培养基中培养小麦赤霉病菌,以100 μg/mL蛇床子素处理,其菌体内14C放射强度比对照降低43%,表明蛇床子素能抑制小麦赤霉病菌对葡萄糖的吸收;以100 μg/mL蛇床子素处理小麦赤霉病菌12 h,菌体内总钙含量仅为对照的60%,表明蛇床子素能抑制菌体对钙的吸收;蛇床子素处理对β-1,6-葡聚糖酶活性没有显著影响;离体条件下,蛇床子素能抑制小麦赤霉病菌三磷酸腺苷(ATP)酶活性。 相似文献
6.
7.
An-Li Wang Wei-Na Wang Yue Wang Li-Xin Shang Yuan Liu Ru-Yong Sun 《Aquaculture (Amsterdam, Netherlands)》2003,220(1-4):833-841
The 96-h LC50 of ammonia-N and the effects of dietary vitamin C on oxygen consumption, ammonia-N excretion and Na+/K+ ATPase activity of Macrobrachium nipponense exposed to ambient ammonia were investigated. The results showed that the 96-h LC50 of ammonia-N was 36.6 mg l−1 for the freshwater prawn, M. nipponense, at pH 8.0. When prawns were exposed to high ambient ammonia-N concentrations, the oxygen consumption rate increased and ammonia excretion decreased. Dietary vitamin C supplementation led to higher oxygen consumption and lower ammonia excretion. Na+/K+ ATPase activity increased with increased ambient ammonia-N exposure in the range of 0–18.3 mg l−1, and then was reduced at ambient ammonia-N 36.6 mg l−1. Na+/K+ ATPase activities of prawns fed a vitamin C-supplemented diet were significantly lower than those of prawns fed a diet which was not supplemented with vitamin C. 相似文献
8.
Freshwater mussel Lamellidens marginalis showed a Ca2+-stimulated ATPase activity in the microsomal fraction of gill. Mg2+ was also found to be an activating cation for this ATPase enzyme. Ca2+ ATPase showed maximal activity at 40 °C and between pH 7.5–8.0. Substrate specificity of Ca2+ ATPase was highest with ATP, followed by GTP and other trinucleotides such as UTP, CTP and ADP also showed some amount of hydrolysis. Ca2+ ATPase showed slight inhibition with ruthenium red and sodium vanadate and is insensitive to sodium azide, ouabain, oligomycin B and phenylalanine. Heavy metals like Hg2+, Cu2+ and Zn2+ showed 50% inhibition of Ca2+ ATPase activity at concentrations of 0.25 mM, 0.5 mM and 1 mM, respectively whereas Cd2+ and Ni2+ showed 39% and 28% inhibition of enzyme activity at 1 mM concentrations. 相似文献
9.
P型ATP酶是植物体内一种重要的膜转运蛋白,在果实能量代谢和酸代谢中发挥重要作用,并广泛参与植物离子运输、抗逆、细胞信号转导等各项生命活动,然而在荔枝或其他无患子科植物中尚未对P型ATP酶基因家族进行全面分析,本研究基于‘妃子笑’荔枝转录组数据,通过对这些基因进行生理生化分析、生物信息学分析和表达分析,共鉴定了28个P型ATP酶基因家族成员基因,探讨P型ATP酶在荔枝采后贮藏过程中的潜在功能。结构和系统发育分析表明该基因可以分为5个亚家族,同一亚族的基因在基因结构和motif基序方面具有很大的相似性,其蛋白二级结构主要以α-螺旋为主,亚细胞定位预测分析发现LcPAs多定位于线粒体(21/28)。通过转录组分析,在采后贮藏期间,荔枝P型ATP酶家族基因中上调表达的基因明显高于下调表达基因,暗示其可能在荔枝采后品质劣变过程中发挥着积极的抵御作用。使用拮抗菌N-1处理荔枝可以有效抑制其果实采后褐变和病害的发生,结合RT-qPCR结果,发现拮抗菌N-1处理能诱导LcPA1、LcPA7、LcPA8和LcPA19在整个贮藏过程中的上调表达,并维持了较高的ATP酶活性,进一步说明P型ATP酶家族基因在抑制荔枝病变过程中发挥着重要作用。本研究为了解荔枝P型ATP酶基因家族基因的进化和功能分析提供了参考,为深入了解拮抗菌N-1保鲜机理的分子调控机理提供新的证据。 相似文献
10.
供铁水平对不同苹果基因型生长及根系特性的影响 总被引:1,自引:0,他引:1
以小金海棠 (MalusxiaojinensisChengetJiang)和山定子 (MalusbaccataL .Borkh)为试材 ,研究供铁水平对其生长及根系特性的影响 ,结果表明 ,在幼苗生长量、新叶叶绿素含量、营养液pH日变化、根系活力、根系ATP酶活性诸方面 ,存在基因型差异。同一供铁水平 ,小金海棠有明显的铁胁迫反应 ,而山定子铁胁迫反应较弱。低铁胁迫处理 ,小金海棠叶片叶绿素含量、根系活力、根系ATP酶活性皆明显高于山定子。根系活力及根系ATP酶活性可用作铁高效基因型植物的筛选指标 相似文献