Immunohistochemical expression of HER - 2 and Ki - 67 in granulosa cell tumor in bitches     

Ana Carina Heil da Silva Matos  Angélica Consalter  Bárbara Paula dos Santos Batista  Ana Beatriz Monteiro Fonseca  Ana Maria Reis Ferreira  Juliana da Silva Leite 《Reproduction in domestic animals》2021,56(4):667-672

Granulosa cell tumour, an ovarian neoplasm of stromal origin, is an important tumour related to oestrogenic dominance syndrome and cystic endometrial hyperplasia–pyometra complex. In order to analyse ovarian tumour´s malignant potential, immunohistochemical markers can be used, such as anti-HER2 and anti-Ki-67. The aim of this study was to evaluate the expression of immunohistochemical markers HER-2 and Ki-67 in granulosa cell tumour from bitches´ ovaries. In HER-2 immunomarker analysis using the HercepTest^® method, most tumours were classified as 2+ (moderate labelling). Concerning Ki-67 immunomarker, only one case was described as having a high proliferative index. An association was found between immunostained cell percentage by anti-HER-2 antibodies and high pleomorphism, represented by the pattern of follicular/trabecular tumour arrangement. There was no correlation between anti-Ki-67 and anti-HER-2 antibody immunostaining intensities, probably due to only one case with a high Ki-67 index. With an effective protocol for HER-2 and Ki-67 immunohistochemical identification in granulosa cell tumours in bitches, it was possible to characterize this neoplasm proliferation profile.  相似文献   

Highly recurrent BRAF p.V595E mutation in canine papillary oral squamous cell carcinoma     

Santiago Peralta  Suzin M. Webb  William P. Katt  Jennifer K. Grenier  Gerald E. Duhamel 《Veterinary and comparative oncology》2023,21(1):138-144

Oral squamous cell carcinoma (OSCC) is the most common oral epithelial malignancy in dogs. It exhibits locally aggressive biological behaviour with the potential to metastasize, and a reported 1-year survival rate of 0% when left untreated. Expression studies suggest that aberrant MAPK signalling plays a key role in canine OSCC tumorigenesis, which is consistent with BRAF and HRAS MAPK-activating mutations reported in some tumours. Several morphological subtypes of canine OSCC have been described, with papillary, conventional, and basaloid as the most common patterns. We hypothesized that mutational differences may underlie these phenotypic variations. In this study, targeted Sanger sequencing and restriction fragment length polymorphism assays demonstrate that up to 85.7% of canine papillary OSCC (n = 14) harbour a BRAF p.V595E mutation. Assessment of neoplastic epithelial cell proliferation using Ki67 immunolabelling (n = 10) confirmed a relatively high proliferation activity, consistent with their known aggressive clinical behaviour. These findings underscore a consistent genetic feature of canine papillary OSCC and provide a basis for the development of novel diagnostic and targeted therapeutic approaches that can improve the quality of veterinary care.  相似文献   

添加纳米粒子的塑料包装材料在杨梅保鲜中的作用   总被引：3，自引：0，他引：3  

张瑶  杨京平  杨莹跃  陆禄  张放 《农机化研究》2007,(3):111-114

纳米技术应用在杨梅保鲜包装上是一个新的尝试,它采用了交互性实验设计方案,将银纳米材料添加进塑料制成包装材料,可观测其保鲜效果.结果表明,纳米塑料包装材料和纳米奇冰石在提高杨梅好果率和延长货架期方面有正面效果;同时,在抑制VC分解方面具有一定的作用.  相似文献   

Evaluation of Caspase-3 and Ki-67 expression in squamous cell hyperplasia of the stomach induced by Platycodi radix water extract in Sprague–Dawley rats     

Yinghua Li  Seung-Beom Cha  Youngil Park  Bo-Ho Gong  In-Yeong Jeong  Hak-Soo Kim  Min-Soo Kang  Young-Suk Kim  Chang Hoon Han  Hyun-Kul Lee  Si-Whan Song  Chae-Gyoo Park  Boo-Hyon Kang 《Journal of toxicologic pathology》2022,35(1):45

Platycodi radix is widely used in traditional herbal medicine for the treatment of bronchitis, asthma, pulmonary tuberculosis, hypertension, hyperlipidemia, and diabetes. This study aimed to investigate cell proliferation (Ki-67) and apoptosis (Caspase-3) potential in squamous cell hyperplasia of the stomach induced by a Platycodi radix water extract in a subchronic toxicity study. One hundred formalin-fixed, paraffin-embedded stomach tissues of rats treated with Platycodi radix at doses of 0, 500, 1,000, and 3,000 mg/kg body weight/day were used for the analysis. They were conventionally stained using hematoxylin and eosin (H&E) and immunohistochemically (IHC) stained using caspase-3 and Ki-67 antibodies. The incidence of squamous cell hyperplasia was significantly increased in the 3,000 mg/kg b.w./day treatment group in both sexes (p<0.01). However, the hyperplastic change was completely repaired after 4 weeks of recovery period. Ki-67 expression was similar in all groups, with no statistically significant differences among the groups. Caspase-3 expression was significantly increased in both sexes in the 3,000 mg/kg b.w./day treatment group (p<0.01), compared with the vehicle control groups, and then reduced to normal levels in the recovery groups in both sexes. In conclusion, this study showed that squamous cell hyperplasia induced by the Platycodi radix water extract in the limiting ridge of the stomach is not considered to be abnormal proliferative change; as a result, squamous cell hyperplasia is considered to be a non-adverse effect when induced by the oral administration of the Platycodi radix water extract once daily for 13 weeks in rats.  相似文献   

铜藻提取液对月见草种子萌发及幼苗生长的影响   总被引：4，自引：0，他引：4  

武冬雪  韩晓弟  邵杰  王磊  王利民  裴侃 《中国林副特产》2008,(5):19-21

研究了不同浓度的铜藻提取液对月见草种子萌发及幼苗生长的影响。结果表明：铜藻提取液可提高月见草种子的萌发率、发芽指数及发芽势,并可促进幼苗的生长。提取液可显著的提高月见草地上部分的干湿重、根系的鲜重、叶片的类胡萝卜素含量及叶绿素a与b的比值;降低叶绿素b的含量。但是,对其根系的干重及叶绿素a的影响规律性较差。总体来讲,铜藻提取液对月见草的种子萌发及幼苗生长具有促进作用。  相似文献   

基于纳米材料电化学免疫传感器检测禽呼肠孤病毒研究   总被引：1，自引：0，他引：1  

黄娇玲  谢芝勋  谢丽基  滕丽琼  黄莉  刘加波  庞耀珊  邓显文  谢志勤  范晴  罗思思 《中国农业科学》2014,47(5):1006-1012

【目的】构建一种用于检测禽呼肠孤病毒(ARV)的新型电化学免疫传感器。【方法】将石墨烯(G)和甲壳胺(Chi)分散在乙酸溶液中得到均匀的混合物后,加入HAuCl4溶液于80℃还原成金纳米粒子（AuNPs）,得到石墨烯-甲壳胺-金纳米粒子(G-Chi-AuNPs)纳米复合物。将石墨烯(G)和甲壳胺(Chi)分散在乙酸溶液中得到均匀的混合物后,先加入AgNO3溶液于80℃还原成银纳米粒子（AgNPs）,再加入禽呼肠孤病毒单克隆抗体(ARV-MAb),得到石墨烯-甲壳胺-银纳米粒子-禽呼肠孤病毒单克隆抗体（G-Chi-AgNPs-ARV-MAb）纳米复合物。将G-Chi- AuNPs纳米复合物修饰金电极作为传感器平台,固定待检测样品,然后加入G-Chi-AgNPs-ARV-MAb纳米复合物,并对G-Chi-AgNPs-ARV-MAb纳米复合物的孵育时间进行优化,构建了ARV电化学免疫传感器。使用构建的ARV电化学免疫传感器,对禽流感（H5N1、H3N6和H9N2亚型）、新城疫病毒（NDV）、喉气管炎病毒（LTV）、传染性支气管炎病毒（IBV）和传染性法氏囊病毒(IBDV)进行检测,以确定ARV电化学免疫传感器的特异性。使用构建的ARV电化学免疫传感器,对106.5-100.5 TCID50/mL的病毒进行检测,以确定ARV电化学免疫传感器的敏感性试验。使用构建的ARV电化学免疫传感器,对临床样品进行检测,其确定ARV电化学免疫传感器的实用性。【结果】所建立的ARV电化学免疫传感器,G-Chi-AgNPs-ARV-MAb纳米复合物的最佳孵育时间为40 min。当检测的样品为阳性时,ARV与ARV-MAb特异结合,G-Chi-AgNPs-ARV-MAb纳米复合物被固定到电极的表面使其线性伏安曲线出现AgNPs的氧化峰;当检测样品为阴性时,其线性伏安曲线不出现AgNPs的氧化峰。特异性结果表明,所建立的方法仅对ARV的检测为阳性（出现AgNPs的氧化峰）,而对非目标禽流感（H5N1、H3N6和H9N2亚型）、NDV、LTV、IBV和 IBDV的检测为阴性（不出现AgNPs的氧化峰）。敏感性结果表明,所构建的ARV电化学免疫传感器,具有很高的敏感性,对ARV检测的敏感性达101.5 TCID50/mL。使用建立的ARV电化学免疫传感器对22份临床样品进行检测,结果与病毒分离方法相符。【结论】所建立的ARV电化学免疫传感器,具有特异性强、敏感度高及快速的特点,为有效检测诊断禽呼肠孤病毒提供了一种新的快速检测诊断技术。  相似文献   

多菌灵杀菌剂对青海弧菌和斑马鱼的急性毒性研究     

熊昭娣  周梦颖  高翔  郭印  谢剑 《安徽农业科学》2017,45(33)

[目的]研究农药对农田环境及非靶标有益生物的影响。[方法]选取青海弧菌Q67和斑马鱼作为受试生物,研究多菌灵杀菌剂对其的急性毒性,从而初步评价该杀菌剂农业生产应用中对水生生物的潜在风险。[结果]22%多菌灵杀菌剂对青海弧菌Q67的EC_(50)为7.70 mg/L,pEC_(50)为2.11;22%多菌灵杀菌剂对斑马鱼的24、48、72、96 h LC_(50)分别为8.53、8.39、8.07和7.64 mg/L;斑马鱼的安全浓度为0.76 mg/L。[结论]根据《化学农药环境安全评价试验准则》"鱼类急性毒性试验"对鱼类毒性评价标准,判断22%多菌灵杀菌剂对斑马鱼的毒性属中毒。  相似文献   

Expression and clinical significance of RhoC and Ki-67 in human esophageal squamous cell carcinoma tissues     

LIN You-en  NI Yang-peng  WANG Xiao-fen  WANG Chun-bing 《园艺学报》2013,29(6):1133-1141

AIM: To investigate the expression and significance of RhoC and Ki-67 in human esophageal squamous cell carcinoma (ESCC) tissues.METHODS: The expression of RhoC and Ki-67 was detected in 52 specimens of ESCC by the method of immunohistochemistry. The clinicopathological features were also analyzed.RESULTS: The expression of RhoC was detected in 32 of the total 52 (61.5%) cases of human ESCC tissues, significantly higher than that in the adjacent histologically normal epithelium, which was only in 11 of 37 cases (29.7%, P<0.05). RhoC expression was closely correlated with clinical tumor-node-metastasis (TNM) stage (P<0.05) and lymph node metastasis (P<0.05) in ESCC. The over-expression of RhoC was positively correlated with Ki-67 in ESCC (r=0.322, P<0.05).CONCLUSION: The over-expression of RhoC protein significantly correlates with advanced TNM stage, lymph node metastasis and cell proliferation ability of ESCC. Therefore, RhoC may be a new auxiliary parameter for early diagnosis and prognostic evaluation of ESCC.  相似文献   

籼三系不育系楠丰67A选育和利用评价研究     

卢华金  杨文清  楼珏  阮柏苗  郑国楚 《安徽农业科学》2012,(36):17505-17507

[目的]选育籼三系不育系楠丰67A,并对其利用价值进行评价.[方法]介绍楠丰67A的选育经过、特征特性、稻米品质遗传特点和制繁种关键技术,探讨其应用价值及潜力.[结果]研究迟熟早籼矮败型不育系楠丰67A,典败率96.78％,柱头外露率43.98％,其中双外露率25.30％.株型好,一般配合力(GCA)效应值总体高于珍汕97A,单株穗数、穗总粒数和结实率比珍汕97A有更强的杂种优势,单株粒重、千粒重的特殊配合力(SCA)效应方差较小,软胶稠度,中低直链淀粉含量,中感稻瘟病.2009年通过浙江省农作物审定委员会组织的技术鉴定.[结论]该研究对今后三系不育系的选育和楠丰67A的利用具有指导意义.  相似文献   

Telomerase activity and telomerase reverse transcriptase catalytic subunit expression in canine lymphoma: correlation with Ki67 immunoreactivity   总被引：1，自引：1，他引：1  

M. G. Renwick  D. J. Argyle  S. Long  C. Nixon  E. A. Gault  L. Nasir 《Veterinary and comparative oncology》2006,4(3):141-150

  相似文献