Genome-wide association mapping of phenolic acids in tetraploid wheats     

《Journal of Cereal Science》2017

Phenolic acids are major components of cell walls in wheat and have important implications on human health as antioxidants with anti-tumor activity. Our objectives were to identify phenolic acid genes in wheat by single nucleotide polymorphisms (SNPs) detected within the coding sequences of candidate genes, and to identify chromosomal regions associated with single phenolic acids and total soluble phenolic compounds. A set of candidate genes involved in the biosynthesis of hydroxycinnamic acid derivatives were identified by comparative genomics. SNPs found in the coding sequences of six genes (PAL1, PAL2, C4H, C3H, COMT1 and COMT2) were used to determine their chromosomal location and accurate map position on two reference consensus linkage maps. The genome-wide association study (GWAS), based on genotyping a tetraploid wheat collection with 81,587 gene-associated SNPs, detected 22 quantitative trait loci (QTL) distributed on almost all durum wheat chromosomes. Two QTL for p-coumaric acid were coincident with the phenylalanine ammonia-lyase (PAL2) and p-coumarate 3-hydroxylase (C3H) genes on chromosome arms 2AL and 1AL, respectively. The availability of candidate gene-based markers can allow elucidating the mechanism of phenolic acids accumulation in wheat kernels and exploiting the genetic variability of phenolic acids content for the nutritional improvement of wheat end-products.  相似文献   

白浆土的氮素分布特征     

丁光伟  赵玉萍  夏荣基 《中国农业大学学报》1990,(Z4):61-70

本文以黑龙江省三江平原耕地白浆土为主要研究对象,并以复区内存在的草甸土和暗棕壤作比较,研究了土壤各种理化性质与不同形态氮素之间的相关关系:白浆土中 HA 和 FA 的主要差别,在于前者含有较多的碱性氨基酸,而后者则含有较多的酸性氨基酸;各种不同形状 N素与土壤 pH 值,质地等相关性不显著,而与土壤有机质、全 N 和 C/N 比值等的相关性极为显著。  相似文献   

南方鲶肌肉,鳔及胡子鲶肌肉的水解和游离氨基酸研究   总被引：2，自引：0，他引：2  

陈定福 《西南大学学报(自然科学版)》1994,16(3):237-239

本试验测定了南方鲶肌内和鳔及胡子鲶肌肉的水解氨基酸和游离氨基酸含量，并对其营养价值进行了比较，评价。  相似文献   

Variations in parameters of liver function and plasma progesterone related to underfeeding and ketosis in a dairy herd   总被引：2，自引：0，他引：2  

E Ropstad  K Halse  A O Refsdal 《Acta veterinaria Scandinavica》1989,30(2):185-197

Twenty-eight Norwegian Red Cattle dairy cows were fed silage ad libitum and restricted amounts of concentrates. Blood samples were collected before morning feeding, once or twice weekly, from 2 weeks before to 12 weeks after calving. Parameters of liver function, carbohydrate status and fertility were recorded in order to assess their interrelationships. Eight cows were treated for clinical ketosis. Four of these had to be treated 2 or 3 times. Aspartate aminotransferase and bilirubin showed the highest within-animal coefficients of correlation with acetoacetate. Analysis of variance revealed a significant effect of carbohydrate status (indicated by plasma acetoacetate levels) on the levels of aspartate aminotransferase, glutamate dehydrogenase and sorbitol dehydrogenase, though only a small part of the total variation was explained by this factor. The estimated volume density of liver fat in the 4th week of lactation averaged 6.0 +/- 6.4% (+/- SD) ranging from 0.1-25.1%. Liver fat content at this stage of lactation was not significantly correlated with other indicators of liver function or carbohydrate status. Cows treated for clinical ketosis had significantly lower plasma progesterone values at the time of first ketosis treatment than untreated multiparous cows. The frequency of high progesterone values (greater than 3 ng/ml) being significantly lower in treated than in untreated cows during the period from 3-5 weeks post partum, though not at later stages. In conclusion, the results revealed a significant relationship between carbohydrate status and liver function, and also between clinical ketosis and luteal function.  相似文献   

Changes in Endopeptidase Activity during Photosynthetic Declination in Rice Leaf     

DENG Zhi-rui ZHANG Rong-xian 《水稻科学》2004,11(1)

Two japonica rice varieties, Wuyujing 3 and 97-7, were used to study the changes in contents of soluble protein, free amino acids and endopeptidase activity during photosynthetic declination. The content of soluble protein in flag leaf of cv.Wuyujing 3 was higher than that of cv. 97-7, but decreased rapidly in Wuyujing 3. Free amino acids in flag leaf and the thirteenth leaf of Wuyujing 3 started to increase 10 days before the turning point of photosynthetic declination (TPPD), while it occurred just 1-2 days before TPPD in the flag leaf and the thirteenth leaf of 97-7. During reversible phase of photosynthetic declination,endopeptidase activity remained at a low level and increased slightly only in the later part of this phase. Then it rose up rapidly at irreversible decline phase and reached a very high level. For Wuyujing 3, the change in endopeptidase activity in the thirteenth leaf was parallel to that in flag leaf. However, for 97-7, the rapid increase of endopeptidase activity in the thirteenth leaf started later than that of flag leaf. The results implied that the rate of protein breakdown and conversion to transportable nitrogen in leaves of 97-7 was slower than that in leaves of Wuyujing 3 during photosynthetic declination and it led to relatively lower seed setting rate and fully filling grains rate in 97-7. This may be one of the important reasons why 97-7 could not bring the high yield potentiality into play and the findings may be taken into consideration while breeding for high potential varieties in future.  相似文献   

不同饲养水平下蛋白质与氨基酸在阉牛小肠内消化率的研究   总被引：3，自引：0，他引：3  

魏宏阳  王加启 《动物营养学报》2002,14(3):18-23

选择6头体重500～600kg ,安装有瘤胃、十二指肠前端和回肠末端瘘管的阉牛 ,分3期饲喂以玉米、棉籽饼和氨化稻草为基础原料配合的3种不同饲养水平的日粮 ,测定十二指肠、回肠和粪便中的粗蛋白质和各种必需氨基酸流量 ,计算粗蛋白质、真蛋白、总必需氨基酸、总非必需氨基酸和各种必需氨基酸在小肠内的表观消化率。结果显示 :随着饲养水平的下降 ,粗蛋白质、真蛋白、总必需氨基酸、总非必需氨基酸和亮氨酸、蛋氨酸、色氨酸、缬氨酸、胱氨酸的小肠表观消化率逐渐降低(P<0.05)。在每种饲养水平下 ,真蛋白的小肠表观消化率均大于粗蛋白质 (P<0.05) ;各种必需氨基酸的小肠表观消化率均有差异 ,精氨酸和亮氨酸的小肠消化率通常比真蛋白高 ,色氨酸和苏氨酸的小肠消化率比真蛋白低 ,但各种必需氨基酸的小肠表观消化率并没有一致的大小顺序。与日粮相比 ,十二指肠中的必需氨基酸组成发生了较大的变化 ;与回肠流量相比 ,粪便中的必需氨基酸排泄量也发生了较大的变化。  相似文献   

三分域模型在研究单胃动物内源氨基酸排泄中的应用   总被引：1，自引：0，他引：1  

陈正玲  明道绪 《动物营养学报》2001,13(2):5-9

本文在分析三分域链状系统模型及单胃动物内源氨基酸排泄特点的基础上 ,讨论了如何将三分域链状模型用于单胃动物内源氨基酸排泄模式的确定。研究分三步进行 :1.测定动物血液体积 ;2.静脉连续灌注同位素标记的氨基酸并使之于体内达到平衡 ;3.检测消化道中同位素标记氨基酸的排泄率。文章指出 ,若能对所有必需氨基酸的内源排泄率均进行测定 ,则可得出内源氨基酸排泄模式 ,因而在实际测定饲料或日粮真可消化氨基酸含量时 ,只需测定某种氨基酸的内源排泄量即可知其它氨基酸的内源排泄状况  相似文献   

Essential Fatty Acids and the Cat     

RICHARD G. HARVEY 《Veterinary dermatology》1993,4(4):175-179

Abstract— The physiology of essential fatty acid metabolism in the cat is reviewed. Emphasis is placed on those aspects of the n:6 and n:3 fatty acids, their metabolites and interactions, which relate primarily to the skin. The functional roles, if known, of the fatty acids are discussed. Recent clinical research into the use of essential fatty acid supplements in the management of feline dermatoses is presented. Current indications for the therapeutic supplementation with essential fatty acids are summarised.  相似文献   

河南小尾寒羊产肉性能与羊肉品质的分析研究     

邹继业  陈自刚  徐廷生  庞有志  王天增  李普宾  刘榜 《河南科技大学学报(农学版)》1994,(1)

就河南小尾寒羊的产肉性能和羊肉品质进行了分析研究。结果表明:河南小尾寒羊生长发育快,产肉性能好,肉质细嫩,肉味浓郁,6月龄的屠宰率较高。肌肉中的蛋白质含量较高,氨基酸含量丰富,其中的赖氨酸、异亮氨酸等达到或超过了标准蛋白质中的含量。  相似文献   

姜片虫虫体抗原氨基酸的测定   总被引：1，自引：0，他引：1  

喻诚沼  马细妹  任园 《江西农业大学学报》1989,(1)

本文报道4种方法制备姜片虫虫体抗原,进行氨基酸组成的测定。其氨基酸总量(mg/100ml)结果显示:Ag-F1:608.50;Ag-F2:645.80;Ag-F3:357.14;Ag-F4:501.40;以 Ag-F3总量为最低,但值得重视的是 Ag-F3的丙氨酸(8.34),组氨酸(8.60),甘氨酸(11.00);苏氨酸(12.00),均显著降低。而 Ag-F4的组氨酸(4.80)为17种氨基酸含量最低者。其原因有待探讨。  相似文献