菌根生物技术应用现状与研究进展   总被引：4，自引：0，他引：4  

蒋家淡  林延生  詹正宜  鲍晓红  刘亨平 《甘肃农业大学学报》2001,36(2):221-225

论述了菌根的类型、功能及其在林业上的应用现状，并指出菌根生物技术研究进展与方向，为林业生产提供参考。  相似文献   

菌根与植被恢复   总被引：5，自引：1，他引：5  

林晓民  李振岐  韩德俊  侯军 《干旱地区农业研究》2003,21(2):167-170

菌根不仅可促进植物的营养吸收、生长发育、抗病、抗逆,而且在保持良好的土壤结构、控制水土流失方面具有直接的作用。在我国西北许多地区,菌根菌与植物间的共生关系已被中断,要恢复该地区的植被,治本的方法应是重建植物与菌根菌的共生关系,形成健康的生态系统。文中还提出了菌根技术在植被恢复中的应用策略。  相似文献   

丛枝菌根真菌对芋组织培养苗生长的影响   总被引：9，自引：0，他引：9  

李敏  刘鹏起  刘润进 《园艺学报》2002,29(5):451-453

 于温室盆栽条件下研究了丛枝菌根(AM) 真菌Gigaspora rosea、Glomus mosseae 和Glomus versi-forme 对芋(Colocasia esculenta) 组织培养苗移栽成活率、矿质营养、光合速率及生长的影响。结果表明, 接种AM真菌能提高芋组织培养幼苗移栽成活率和叶片光合速率, 降低气孔阻力; 其叶片和根内氮、磷、钾含量和生长量显著高于不接种对照。认为接种有效AM真菌是促进组织培养苗健康生长的重要技术。  相似文献   

Community structure of arbuscular mycorrhizal fungi associated with Robinia pseudoacacia in uncontaminated and heavy metal contaminated soils     

《Soil biology & biochemistry》2015

The significance of arbuscular mycorrhizal fungi (AMF) in soil remediation has been widely recognized because of their ability to promote plant growth and increase phytoremediation efficiency in heavy metal (HM) polluted soils by improving plant nutrient absorption and by influencing the fate of the metals in the plant and soil. However, the symbiotic functions of AMF in remediation of polluted soils depend on plant–fungus–soil combinations and are greatly influenced by environmental conditions. To better understand the adaptation of plants and the related mycorrhizae to extreme environmental conditions, AMF colonization, spore density and community structure were analyzed in roots or rhizosphere soils of Robinia pseudoacacia. Mycorrhization was compared between uncontaminated soil and heavy metal contaminated soil from a lead–zinc mining region of northwest China. Samples were analyzed by restriction fragment length polymorphism (RFLP) screening with AMF-specific primers (NS31 and AM1), and sequencing of rRNA small subunit (SSU). The phylogenetic analysis revealed 28 AMF group types, including six AMF families: Glomeraceae, Claroideoglomeraceae, Diversisporaceae, Acaulosporaceae, Pacisporaceae, and Gigasporaceae. Of all AMF group types, six (21%) were detected based on spore samples alone, four (14%) based on root samples alone, and five (18%) based on samples from root, soil and spore. Glo9 (Rhizophagus intraradices), Glo17 (Funneliformis mosseae) and Acau3 (Acaulospora sp.) were the three most abundant AMF group types in the current study. Soil Pb and Zn concentrations, pH, organic matter content, and phosphorus levels all showed significant correlations with the AMF species compositions in root and soil samples. Overall, the uncontaminated sites had higher species diversity than sites with heavy metal contamination. The study highlights the effects of different soil chemical parameters on AMF colonization, spore density and community structure in contaminated and uncontaminated sites. The tolerant AMF species isolated and identified from this study have potential for application in phytoremediation of heavy metal contaminated areas.  相似文献   

Molecular analysis of the Arbuscular mycorrhiza symbiosis     

Philipp Franken  Natalia Requena  Britta Bütehorn  Franziska Krajinski  Gerrit Kuhn  Laurence Lapopin 《Archives of Agronomy and Soil Science》2013,59(4):271-286

The molecular analysis of the arbuscular mycorrhizal (AM) symbiosis started at the beginning of this decade. The paper summarises the work of the ‘Laboratorium für Molekulare Genetik arbuskulärer Mykorrhiza’ at the MPI für terrestrische Mikrobiologie in Marburg on three major topics. Firstly, the plant response to the mycorrhizal colonisation was analysed using both, targeted and non‐targeted approaches. As an example, the localisation of the gst1 mRNA in potato mycorrhiza is shown. Second, molecular techniques were established to analyse gene expression of the fungal partner of the symbiosis. We present a differential RNA display analysis of spore germination in two AM fungi and the cloning of a gene from Glomus mosseae that shows expression at all stages of the fungal life cycle. In the last part, we introduce the work we are carrying out with a new root endophytic fungus, Piriformospora indica. Infection experiments on maize showed that despite the fungus performs as a root necrotroph, it has a positive effect on plant growth.  相似文献   

Effect of tillage and crop on arbuscular mycorrhiza colonization of winter wheat and triticale under Mediterranean conditions     

I. Brito  M. J. Goss  M. De Carvalho 《Soil Use and Management》2012,28(2):202-208

Large‐scale inoculation with arbuscular mycorrhizal fungi (AMF) is generally impractical in most regions and we have little understanding of the factors that determine inoculation success. Nevertheless, the ability to take full advantage of indigenous AMF for sustainable production needs to be developed within cropping systems. We used part of a long‐term field experiment to understand the influence of tillage and the preceding crop on AMF colonization over the growing season. Arbuscular mycorrhiza colonization rate was more affected by treatment (tillage or the combination of crop and preceding crop) than by the total number of AMF spores in the soil. Conventional tillage (CT) had a statistically significant negative effect (P ≤ 0.05) on spore numbers isolated from the soil, but only in the first year of study. However, the AMF colonization rate was significantly reduced by CT, and the roots of wheat, Triticum aestivum, L, cv. Coa after sunflower, Helianthus annuus L., were less well colonized than were those of triticale, X Triticosecale Wittmack, cv. Alter after wheat, but the affect of tillage was more pronounced than was the effect of crop combination. Under no‐till there was a significant increase in AMF colonization rate throughout the sampling period in both wheat and triticale, indicating that the extraradical mycelium previously produced acted as a source of inoculum. In general, triticale showed greater AMF colonization than wheat, despite the preceding crop being less mycotrophic. Under these experimental conditions, typical of Mediterranean agricultural systems, AMF colonization responded more strongly to tillage practices than to the combination of crop and preceding crop.  相似文献   

Protection of olive planting stocks against parasitism of root-knot nematodes by arbuscular mycorrhizal fungi   总被引：3，自引：0，他引：3  

P. Castillo  A. I. Nico  C. Azcón-Aguilar  C. Del Río Rincón  C. Calvet  R. M. Jiménez-Díaz 《Plant pathology》2006,55(5):705-713

The effects were investigated, under controlled conditions, of single and joint inoculation of olive planting stocks cvs Arbequina and Picual with the arbuscular mycorrhizal fungi (AMF) Glomus intraradices , Glomus mosseae or Glomus viscosum , and the root-knot nematodes Meloidogyne incognita and Meloidogyne javanica , on plant performance and nematode infection. Establishment of the fungal symbiosis significantly increased growth of olive plants by 88·9% within a range of 11·9–214·0%, irrespective of olive cultivar, plant age and infection by M. incognita or M. javanica . In plants free from AMF, infection by Meloidogyne spp. significantly reduced the plant main stem diameter by 22·8–38·6%, irrespective of cultivar and plant age. Establishment of AMF in olive plants significantly reduced severity of root galling by 6·3–36·8% as well as reproduction of both Meloidogyne spp. by 11·8–35·7%, indicating a protective effect against parasitism by root-knot nematodes. Infection by the nematodes influenced root colonization by AMF, but the net effect depended on the AMF isolate–olive cultivar combination. It is concluded that prior inoculation of olive plants with AMF may contribute to improving the health status and vigour of cvs Arbequina and Picual planting stocks during nursery propagation.  相似文献   

Responses of field grown tomato plants to arbuscular mycorrhizal fungal colonization under varying intensities of drought stress     

K.S. Subramanian  P. SanthanakrishnanP. Balasubramanian 《Scientia Horticulturae》2006

The effects of root colonization by the arbuscular mycorrhizal (AM) fungus Glomus intraradices Schenck and Smith on growth, flower and fruit production, and fruit quality were studied in field-grown tomato plants exposed to varying intensities of drought stress. Inoculated (M+) and non-inoculated (M−) tomato seedlings were exposed to varying intensities of drought stress by adjusting irrigation intervals. Mycorrhizal plants had significantly higher uptake of N and P in both roots and shoots regardless of intensities of drought stress. AM inoculation also significantly increased shoot dry matter and the number of flowers and fruits. The fruit yields of M+ plants under severe, moderate, mild drought-stressed conditions were higher than M− plants by 24.7%, 23.1%, 16.2% and 12.3%, respectively. Furthermore, M+ plants produced tomato fruits that contain significantly higher quantities of ascorbic acid and total soluble solids (TSS) than M− plants. Mycorrhizal effects increased with increasing intensity of drought. The overall results suggest that mycorrhizal colonization affects host plant nutritional status, water stratus and growth under field conditions and thereby alters reproductive behaviour, fruit production and quality of fruits under both well-watered and drought-stressed conditions.  相似文献   

放牧因子与VA菌根的相互作用对鸭茅养分吸收和生长发育的影响   总被引：1，自引：0，他引：1  

乌恩  菅原和夫  云锦凤 《干旱区资源与环境》2007,21(11):136-140

探讨了放牧因子与VA菌根的相互作用对鸭茅(Dactylis glomerataL.)养分吸收和生长发育的影响。菌根效应表现在不施磷肥条件下的鸭茅地上部生育与地上、地下部的磷素营养上,但对地上部生育的效应因刈割次数的增加而降低,对地上、地下部磷素营养的效应因土壤镇压而降低。菌根对鸭茅钙素营养的效应只见于不施磷肥条件下的土壤镇压处理,而在相同条件下菌根却降低了鸭茅的镁含量。说明VA菌根对鸭茅磷素营养和生长发育的效应与土壤磷营养有关,而且因地上部的过度采食和土壤镇压而降低。  相似文献   

Distribution of Arbuscular Mycorrhizal Fungi in Upland Field Soil of Japan 1. Relationship Between Spore Density and The Soilenvironmental Factor     

《Plant Production Science》2013,16(1):122-128

Abstract

To Quantify The Effects of Arbuscular Mycorrhizal Fungi (Am Fungi) On The Growth of Upland Field Crops Cultivated in Japan, We Analyzed Soil Samples From 124 Sites in 18 Japanese Prefectures For Available P Content, Ph and Am Fungal Spore Density. The Am Fungal Spore Density in The 124 Soil Samples Was 1.7 Per G Dw On The Average, and Lower Than 1.0 Per G Dry Soil (Dw) in About Half of The Soil Samples. The Maximum Spore Density Was 20.6 Spores Per G Dw. The Density of Am Fungal Spore Did Not Vary Significantly With The Sampling Site and The Kind of Cultivated Crop in The Sampling Field. The Ph of The Soil With A High Spore Density Ranged From 6 To 8, and in The Soil Samples With A Ph Lower Than 6 and Higher Than 8, TheSpore Density Was Lower Than 5 Spores Per G Dw. Thus, in The Acid Or Alkaline Soil, The Sporogenesis of Am Fungi Is Suppressed. Because Available P Content Was Consistently Low in The Soil Samples With A High Spore Density, P Content Was Considered To Correlate With The Am Fungal Spore Density. Therefore, Crop Cultivation With Limited P Fertilizer Application and Reduced Available P Content May Be Important To increase Am Fungal Spore Density in Upland Field Soil.  相似文献