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为了解正安县山羊主要疫病的发展趋势,通过虎红平板凝集试验、正向间接血凝试验、酶联免疫吸附试验等方法对正安县引进山羊进行布鲁氏杆菌病、O型口蹄疫、AsiaI型口蹄疫、山羊传染性胸膜肺炎、山羊痘等主要疫病血清抗体的检测。结果表明,O型口蹄疫、AsiaI型口蹄疫和山羊痘免疫抗体阳性率分别为79.42%、81.18%和99.58%,未免疫的布鲁氏杆菌病、羊传染性胸膜肺炎的丝状支原体山羊亚种和绵羊肺炎支原体未免疫抗体阳性率分别为0%、0%和3.81%。监测结果为山羊健康的引进、培育和扩繁提供了一些基础数据,对指导养羊生产有一定的意义。  相似文献   
2.
The study was conducted in two selected districts of Southern Omo zones of Ethiopia, namely Hammer and Benna-Tsemay, during November 2004 and May 2005 to determine the status of contagious caprine pleuropneumonia (CCPP). Participatory disease investigation was conducted in the goat flocks owned by pastoralists of the districts. Participatory methods such as proportionate piling and matrix scoring of diseases were used to characterise major diseases of goats. Clinical and post-mortem examinations and isolation of the causative agent of CCPP were done. Serological tests were conducted using CFT. CCPP (locally termed Sompo) ranked as the first important disease of goats in the study area. Local perception of causes and signs of CCPP were described. Matrix scoring between groups revealed that disease signs and causes showed weak, moderate and good agreement by Kendall’s coefficient concordance (W = 0.21–0.99). The overall sero-prevalence of CCPP was 15.5%. The causative agent was isolated from sick animals in the lab. The characteristic clinical signs, gross lesions, bacteriological isolation of the causative agent supported by participatory epidemiological disease investigation revealed that CCPP is a major disease of goats in the study districts. Participatory epidemiology using indigenous knowledge could efficiently be used to generate sufficient information with minimum cost, local materials and within reasonably short period of time, assisting the designing of feasible disease control programme in developing countries.  相似文献   
3.
山羊传染性胸膜肺炎继发大肠埃希氏菌感染的诊断   总被引:2,自引:0,他引:2  
2010年立秋以来,贵州省众多山羊养殖场羊群出现咳嗽、高热等疑似山羊传染性胸膜肺炎(CCPP)的症状,为查明致病原因,以便及时采取针对性治疗措施,防止疫情蔓延,采用生物学试验对临床送检病料进行了CCPP的病原鉴定及细菌分离鉴定,并对分离菌进行了药敏试验.结果表明,CCPP主要致病原Mo检出率为44.4%(4/9),是首...  相似文献   
4.
Mycoplasmas were isolated from freeze-dried lung samples from goats from the western lowlands of Eritrea suspected of being affected by contagious caprine pleuropneumonia. The goats belonged to two herds in which mortality and morbidity rates were high. Mycoplasma capricolum subsp. capripneumoniae was identified in most samples by the polymerase chain reaction and by conventional serological tests. The latex agglutination test detected more positive serum samples in both herds than did the complement fixation test. Following cloning, the isolates of M. capricolum subsp. capripneumoniae were analysed biochemically and shown to be metabolically similar. They oxidized glucose, N-acetylglucosamine, pyruvate and L-lactate with high affinity and mannose, glucosamine and 2-oxobutyrate with low affinity; they were unable to utilize maltose, trehalose, fructose or ethanol. Major improvements were seen in the growth yield of the Eritrean strains with the addition of pyruvate to the medium. Thus, it may be that organic acids are important energy sources for these strains and may be used in addition to or in place of glucose. In contrast to most other strains of the M. mycoides cluster, the Eritrean strains produced large amounts of hydrogen peroxide during the oxidation of NADH by lysed cells. This characteristic had previously been reported for strain M. F38, the type strain of M. capricolum subsp. capripneumoniae, although strain F38 did not metabolize sugars. Hydrogen peroxide has long been considered a pathogenicity factor in mycoplasma infections. This is the first isolation of M. capricolum subsp. capripneumoniae from Eritrea.  相似文献   
5.
Livestock export is vital to the Somali economy. To protect Somali livestock exports from costly import bans used to control the international spread of disease, better certification of livestock health status is required. We performed quantitative risk assessment and cost-effectiveness analysis on different health certification protocols for Somali livestock exports for six transboundary diseases.  相似文献   
6.
300 goat serum samples from an export-oriented abattoir were tested for contagious caprine pleuropneumonia antibodies by the complement fixation test. The disease prevalence was 31% with no significant differences (P > 0.05) between the regions “Borena”, “Bale”, “Afar” and “Jinka” or the age of the goats (P > 0.05). Gross pathology and histopathology of the lung primary lesions were indicative of pleuropneumonia caused by Mycoplasma capricolum subsp. capripneumoniae.  相似文献   
7.
构建山羊传染性胸膜肺炎DNA疫苗的展望   总被引:1,自引:0,他引:1  
20世纪90年代以来研究诞生的DNA疫苗以经济适用、制作简易、免疫高效及安全可靠为优点,已迅速成为动物疾病预防的研究重点,被称作"疫苗的第三次革命"。文章要概述了DNA疫苗的特点及国内外研究进展,并从山羊传染性胸膜肺炎病原抗原基因研究方面讨论了构建该病DNA疫苗的意义与重要性,对构建山羊传染性胸膜肺炎DNA疫苗作一展望。  相似文献   
8.
The efficacy of danofloxacin (Advocin A180) was evaluated for the treatment of contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subsp. capripneumoniae. Ten healthy Angora goats, confirmed free of CCPP, were exposed to clinically affected animals from a natural outbreak in Thrace, Turkey. After 14 days exposure, 8 goats showed pyrexia ( > or = 41 degrees C). Shortly after, the Angora goats were divided randomly into two groups. Five of these were injected with danofloxacin (6 mg/kg subcutaneously), which was repeated after 48 h; the five remaining animals received saline. Goats were monitored clinically and blood samples were collected for serology. Animals with severe disease were withdrawn from the trial. Goats completing the study were euthanized at day 42. Lung tissue and bronchial fluid were collected for mycoplasma isolation. All danofloxacin-treated goats showed resolution of clinical disease by the end of the trial. Two saline-treated goats failed to complete the study owing to CCPP. Danofloxacin-treated goats showed fewer lung lesions and had significantly lower combined clinical scores than saline controls (p < 0.001). Danofloxacin was found to be highly effective in the treatment of CCPP in goats.  相似文献   
9.
山羊传染性胸膜肺炎病例分子病原学诊断   总被引:2,自引:0,他引:2  
对贵州省某规模化养羊场出现疑似山羊传染性胸膜肺炎(CCPP)症状的山羊采用分子生物学方法进行CCPP病原核酸检测,并通过生物信息学分析对病原进行确定性诊断。结果表明,本次疫情确诊为CCPP,临床病料经分子生物学和生物信息学分析认为病原基因与绵羊肺炎支原体(Mo)Y98标准株在分子进化上一致,表明引起该场CCPP的病原为...  相似文献   
10.
A cross sectional study was conducted to determine the sero-prevalence of contagious caprine pleuroneumonia in three districts of Tigray and Afar regions of Ethiopia namely; Kefta Humera, Alamata and Aba-‘alla. Proportions and chi-square test statistics were used to analyze the data. From a total of 863 goats and 137 sheep tested, 282 (32.68%) and 25 (18.25%) were positive for antibodies of Mycoplasma capricolum subsp. capripneumoniae respectively using complement fixation test (CFT). The seroprevalence of CCPP in goats among the three districts was statistically significant (x2 = 76.00, p < 0.001). In this study there was no statistical significant variation in the seroprevalence of CCPP in both sexes (x2 = 3.619, p = 0.0571) and age (x2 = 0.990, p = 0.095) groups. The finding of high seroprevalence of CCPP in sheep (18.25%) could indicate that sheep are potential carriers of Mccp.  相似文献   
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