鸡空肠弯曲菌灭活苗的制备及其免疫保护试验研究     

李广兴  刘建平  周志勇 《动物医学进展》2003,24(3):65-66

用分离到的空肠弯曲菌制备鸡空肠弯曲菌甲醛灭活疫苗 ,并进行了该病的免疫保护实验。制备 2× 10 9个 / m L浓度的细菌悬液 ,4m L / L浓度的甲醛溶液作用 12 h灭活后制成空肠弯曲菌灭活苗 ,免疫 2周龄的 SPF雏鸡 ,14 d后 ,进行攻击实验 ,观察其临床症状 ;并分别于感染后 7d和 14 d捕杀 ,观察病理学变化及进行细菌分离实验以揭示其保护率。结果表明 ,鸡空肠弯曲菌灭活苗的保护率可达 10 0 % ,说明该苗经口免疫后安全可靠 ,无毒副作用  相似文献   

Campylobacter spp., Salmonella spp., Verocytotoxic Escherichia coli,and Antibiotic Resistance in Indicator Organisms in Wild Cervids     

A Lillehaug  B Bergsj?  J Schau  T Bruheim  T Vik?ren  K Handeland 《Acta veterinaria Scandinavica》2005,46(1):23-32

Faecal samples were collected, as part of the National Health Surveillance Program for Cervids (HOP) in Norway, from wild red deer, roe deer, moose and reindeer during ordinary hunting seasons from 2001 to 2003. Samples from a total of 618 animals were examined for verocytotoxic E. coli (VTEC); 611 animals for Salmonella and 324 animals for Campylobacter. A total of 50 samples were cultivated from each cervid species in order to isolate the indicator bacterial species E. coli and Enterococcus faecalis/E. faecium for antibiotic resistance pattern studies. Salmonella and the potentially human pathogenic verocytotoxic E. coli were not isolated, while Campylobacter jejuni jejuni was found in one roe deer sample only. Antibiotic resistance was found in 13 (7.3%) of the 179 E. coli isolates tested, eight of these being resistant against one type of antibiotic only. The proportion of resistant E. coli isolates was higher in wild reindeer (24%) than in the other cervids (2.2%). E. faecalis or E. faecium were isolated from 19 of the samples, none of these being reindeer. All the strains isolated were resistant against one (84%) or more (16%) antibiotics. A total of 14 E. faecalis-strains were resistant to virginiamycin only. The results indicate that the cervid species studied do not constitute an important infectious reservoir for either the human pathogens or the antibiotic resistant microorganisms included in the study.  相似文献   

应用PCR技术检测空肠弯曲菌及结肠弯曲菌的研究     

王振国  刘金华  罗雁菲  蔡阳  肖成蕊  刘和平  徐宝梁  王岸英 《中国家禽》2005,9(Z1):133-135

本研究根据空肠弯曲菌及结肠弯曲菌的16s rRNA基因相同保守序列来设计引物,建立了一种PCR检测方法,该方法具有较强的特异性及灵敏性,其对空肠弯曲菌及结肠弯曲菌的最低检测限度为5 CFU/mL,它无需增菌培养过程可以直接对样品进行检验,在对鸡肉样品空肠弯曲菌及结肠弯曲菌的检测中取得了较好的效果.  相似文献   

空肠弯曲菌共同抗原单克隆抗体的研制及其在细菌检验中的应用 Ⅱ.杂交瘤细胞株的建立及特性鉴定     

韩文瑜  黎诚耀  王世若  潘荆华  腾加波  李秀芝  张权一 《中国兽医学报》1990,(1)

应用B-淋巴细胞杂交瘤技术,建立了5株稳定分泌抗空肠弯曲菌共同抗原(CA)单克隆抗体(McAb)的杂交瘤细胞株(4A7,9D6、9C2、11A1、11D4)。在5株杂交瘤分泌的McAb中,有4株(4A7、9D6、11A1、11D4)针对CA中的耐热抗原成分,对其中4A7、9D6两株分泌的McAb进行免疫印迹分析表明,其与CA中大约为7.7×104u的蛋白移行带出现特异反应;一株(9C2)针对CA中的不耐热抗原成分。对杂交瘤细胞的核型分析表明,其染色体数在93-108之间。体外传代4个月,仍保持稳定分泌抗体的特性。这种McAb具有较强的特异性,除与幽门弯曲菌出现弱阳性反应外,与参试的其它31种非弯曲菌属的细菌均为阴性反应。对不同地区、不同来源的516株空肠弯曲菌的鉴定表明,制备的McAb与现有空肠弯曲菌均出现阳性反应,与常规鉴定法的符合率为100％。  相似文献   

空肠弯曲杆菌生物学特性及分型技术研究新进展     

翟海华  王娟  王君玮 《中国畜牧兽医》2014,41(2):223-228

Campylobacter jejuni (C.jejuni) is a major pathogen of human campylobacteriosis, as well as one of the major food-borne pathogen. It can not only cause human diarrhea, but also cause many systemic diseases such as endocarditis, arthritis, septisis. The most serious complication it can cause is the Guillain Barre syndrome. In developed countries, C.jejuni even has a trend to excess Salmonella and be reported as next Salmonella, which has caused a worldwide concern. Analysis of biological characteristics for C.jejuni can help us to distinguish it with other pathogens. Typing methods play important roles in the identification, monitoring and prevention of C.jejuni. It can also help us to understand its virulence and resistance mechanisms to C.jejuni. This paper reviews advances of C.jejuni in biological characteristics, serotyping and genotyping methods so as to help us further understand its new progress in these areas.  相似文献   

空肠弯杆菌研究现状     

陈晓莹  徐自忠  高洪  花群义  周晓黎 《勤云标准版测试》2005,(3)

空肠弯杆菌是一种人兽共同感染的病原菌,也是导致腹泻最常见的病菌,主要症状是发热和急性肠炎。主要从病原的生物学特征、肠毒素的研究、人畜带菌率及流行病学的研究现状进行综合讨论。  相似文献   

Distribution of Campylobacter jejuni isolates from turkey farms and different stages at slaughter using pulsed-field gel electrophoresis and flaA-short variable region sequencing     

Perko-Mäkelä P  Alter T  Isohanni P  Zimmermann S  Lyhs U 《Zoonoses and public health》2011,58(6):388-398

The aim of this study was to assess the diversity of thermotolerant Campylobacter spp. isolated from turkey flocks at six rearing farms 1-2 weeks prior to slaughter (360 faecal swab samples) and from 11 different stages at the slaughterhouse (636 caecal, environmental, neck skin and meat samples). A total of 121 Campylobacter isolates were identified to species level using a multiplex PCR assay and were typed by pulsed-field gel electrophoresis (PFGE) and flaA-short variable region (SVR) sequencing. All Campylobacter isolates were identified as Campylobacter jejuni. PFGE analysis with KpnI restriction enzyme resulted in 11 PFGE types (I-XI) and flaA SVR typing yielded in nine flaA-SVR alleles. The Campylobacter-positive turkey flocks A, C and E were colonized by a limited number of Campylobacter clones at the farm and slaughter. The present study confirms the traceability of flock-specific strains (PFGE types I, V and IX; flaA types 21, 36 and 161) from the farm along the entire processing line to meat cuts. It seems that stress factors such as high temperature of the defeathering water (54-56 °C), drying of the carcass skin during air chilling (24 h at 2 °C), and oxygen in the air could not eliminate Campylobacter completely. Campylobacter-negative flocks became contaminated during processing by the same subtypes of Campylobacter introduced into the slaughter house by preceeding positive flocks even if they were slaughtered on subsequent days. Proper and efficient cleaning and disinfection of slaughter and processing premises are needed to avoid cross-contamination, especially in countries with a low prevalence of Campylobacter spp. The majority of flaA SVR alleles displayed a distinct association with a specific PFGE type. However, a linear relationship for all strains among both typing methods could not be established. To specify genetic relatedness of strains, a combination of different genotyping methods, is needed.  相似文献   

Antimicrobial Resistance and Virulence Genes of Campylobacter spp. Isolated from Chicken and Swine Farms in Jiangsu Province     

TANG Mengjun  ZHOU Qian  ZHANG Xiaoyan  ZHANG Jing  TANG Xiujun  LU Junxian  ZHOU Sheng  PU Junhua  GAO Yushi 《畜牧兽医学报》1956,51(9):2284-2292

The aim of this study was to investigate the antimicrobial resistance, virulence genes of Campylobacter spp. isolated from chicken and swine farms in Jiangsu Province. Campylobacter spp. were isolated and identified from two hundred and fifty fecal samples collected from twenty-five animal farms in Jiangsu Province. Campylobacter strains were tested for the antimicrobial susceptibility against to 9 kinds of antimicrobial agents by using an agar dilution method. Eight virulence genes (cdtB, cadF, htrB, clpP, csrA, wlaN, cstⅡ, and cgtB) were detected by polymerase chain reaction. Ninety-three Campylobacter strains were isolated and identified from 250 samples, including 45 C. jejuni strains and 48 C. coli strains. The highest percentage of antimicrobial resistance was found for nalidixic acid (80.0%), tetracycline (71.1%) and ciprofloxacin (66.7%) in C. jejuni isolates. The C. coli isolates were most frequently resistant to erythromycin (87.5%), nalidixic acid (79.2%) and azithromycin (72.9%). Moreover, the multi-drug resistance (resistance to three or more antimicrobial classes) was common among Campylobacter isolates with a rate of 67.7%. On the other hand, the 93 Campylobacter strains showed a wide variation for the presence of the 8 virulence genes, cdtB and cadF were positive for all isolates,while htrB, clpP, csrA, wlaN, cstⅡ and cgtB was 97.8%, 76.3%, 18.3%, 5.4%, 2.2%, and 0%, respectively. The results indicated that the multiple drug resistance of Campylobacter strains from animal origin was relatively serious. In addition, the virulence-associated genes were detected widely among Campylobacter strains.  相似文献   

Comparison of Four Enrichment Media in the Recovery of Campyloracter Jejuni     

Marja-Liisa Hnninen 《Acta veterinaria Scandinavica》1982,23(3):425

The effectiveness of 4 enrichment media for the recovery of low levels of inoculated cells of Campylobacter jejuni was evaluated. The media contained antibiotics or antibiotics and bile acids as selective compounds. Three of the media recovered most of the inoculated low numbers of 6 C. jejuni strains. In the 3 media the growth rate of 3 strains, indicated by the increase in the log number of cells during 24 h or 48 h incubation at 42 ° C, was about the same as in the control medium without selective compounds. The same 3 media also recovered a low number of Campylobacter cells from artificially contaminated raw milk or ground meat samples. The enrichment medium B containing 40 I.U. Colistin, 5 μg novobiocin, 2 mg Na-cholic acid and 50 mg cycloheximide per ml was inhibitory for most Campylobacter strains studied.  相似文献   

Multilocus Sequence Types of Environmental Campylobacter jejuni Isolates and their Similarities to those of Human,Poultry and Bovine C. jejuni Isolates     

C. P. A. de Haan  K. Lampén  J. Corander  M.‐L. Hänninen 《Zoonoses and public health》2013,60(2):125-133

In this study, we investigated the multilocus sequence type (MLST) diversity and population genetics of Campylobacter jejuni isolates collected from the natural waters (n = 57), wild birds (n = 37) and zoo animals (n = 19) in southern Finland, the Helsinki area and the Helsinki Zoo, respectively. On average, we found C. jejuni in 20%, 10.4% or 11.5% of the samples collected from natural waters, wild birds and zoo animals, respectively. High ST diversity was detected in all three sources and 41.2% of the STs were novel, but the multi‐host adapted ST‐45 was the most common ST detected. The MLST data, supplemented with C. jejuni isolates from domestically acquired human infections (n = 454), poultry (n = 208) and bovines (n = 120), were utilized in a population structure study. The results indicate four groups of strains with varying ecological associations, demonstrating presence of genetically distinct lineages within each of the studied sources. We discovered that the greatest ST overlap occurs between human isolates and isolates from natural waters and poultry, which suggests that the latter two are the most important sources of C. jejuni among domestically acquired infections in Finland.  相似文献