利用甜高粱秸秆汁产单细胞蛋白的分批补料发酵研究     

庞会忠  叶亚健  王飞  程新  吴晓玉  李昆太 《中国饲料》2012,(3):19-21

本文以甜高粱秸秆汁为主要的发酵原料,对热带假丝酵母SG-1在15-L罐上产单细胞蛋白(SCP)的分批补料发酵过程进行研究。首先对分批发酵模式下的菌体代谢过程变化进行研究。研究发现,接种后菌体快速进入繁殖阶段,但是总糖在12 h处于耗竭状态,使得菌体量和SCP产量过早的在18 h就达到峰值。基于此,在15-L罐上建立了热带假丝酵母SG-1产SCP的分批补料发酵模式。结果表明:菌体代谢因基质的补加而得到进一步的延续,放罐时(42 h)其菌体干重和SCP产量分别达19.86 g/L和9.93 g/L,显著高于分批发酵模式的10.45 g/L和4.70 g/L。  相似文献   

大熊猫铜绿假单胞菌呼吸道感染继发白色念珠菌性肠炎的诊治     

修云芳  邵良平  陈玉村  王德春  徐素慧 《经济动物学报》2006,10(2):103-105

报道了1只大熊猫呼吸道感染,继发肠炎的病例。介绍了其病史,血液学变化和临床症状。经过微生物培养和鉴定,确诊呼吸道感染的致病菌为铜绿假单胞菌,继发性肠炎的致病菌为白色念珠菌。根据药物敏感试验结果,选用阿奇霉素和环丙沙星治愈呼吸道感染;选用氟康唑和金双歧,治愈继发性肠炎。并讨论了铜绿假单胞菌和白色念珠菌的发病原因,总结了临床用药的经验。  相似文献   

利用假丝酵母进行棉仁饼固体发酵的培养基筛选   总被引：4，自引：0，他引：4  

孙建义  许梓荣 《浙江大学学报(农业与生命科学版)》1998,(6)

以棉仁饼为原料,热带假丝酵母ＺＡＵ－１为菌种,采用固体发酵技术,对无游离棉酚的酵母饲料进行了研究,探讨了不同原料对酵母饲料发酵的影响,筛选出较合理的固体发酵培养基：棉仁饼６０％,大米蛋白粉２５％,麸皮１０％,饴糖２％,酒糟１％,ＭｇＳＯ４５Ｈ２Ｏ０．２５％,ＫＨ２ＰＯ４０．７５％,ＦｅＳＯ４７Ｈ２Ｏ０．８０％,ＺｎＳＯ４７Ｈ２Ｏ０．２０％,ＭｎＳＯ４Ｈ２Ｏ０．０６％,ＣｕＳＯ４５Ｈ２Ｏ０．０４％和ＣｏＣｌ２６Ｈ２Ｏ０．０２％．  相似文献   

白灰毛豆和新银合欢水提液对白灰毛豆化感作用研究     

赵怀宝  孙丽颖  羊金殿  张孟锦  罗广辉  范梓君  陈菁菁 《种子》2019,(2):46-51

配置4种不同浓度的白灰毛豆、新银合欢的鲜茎叶和凋落叶的水提液,测定其对白灰毛豆种子萌发和幼苗生长的影响。探究白灰毛豆、新银合欢鲜茎叶和凋落叶水浸提液对白灰毛豆的化感作用,为优势种群的形成、群落的演替、植被的恢复等提供依据。结果表明,白灰毛豆、新银合欢鲜茎叶和凋落叶均有明显的化感作用。对种子萌发总体表现为低浓度促进,高浓度抑制。新银合欢水提液对白灰毛豆幼苗生长均表现为抑制作用,且浓度越高抑制作用越强。新银合欢的化感作用可能是群落演替中白灰毛豆被新银合欢快速替代的原因之一。  相似文献   

Study on Antimicrobial and Antiviral Activities of Lysozyme From Marine Strain S-12-86 In Vitro     

ZHANG Xiu  WU Fa-xing  SUN Mi  WANG Qing-yin  WANG Yue-jun  YANG Xiang-ke 《中国农业科学(英文版)》2008,7(1):112-116

In this study, the in vitro antimicrobial and antiviral activities of the lysozyme from marine strain S-12-86 （LS） were investigated, The antimicrobial activity of LS was tested by minimum inhibition concentration （MIC） and minimum bactericidal concentration （MBC） method. The inhibiting effects of LS on pseudo rabies virus （PRV） in swine kidney cells （PK-15 ceils） were judged by cytopathogenic effect test （CPE）, The results showed LS had a broad antimicrobial spectrum against several standard strains including gram-positive bacteria, gram-negative bacteria, fungi, etc, The MIC of LS was 0.25-4.00 mg mL^-1 and its MBC was 0.25-8.00 mg mL^-1, respectively, Observation under the transmission electron microscope revealed that the cell wall of Candida albicans was distorted seriously, and the cytoplasm with many cavities was asymmetrical after being hydrolyzed by LS, The median cytotoxicity concentration （TC50） of LS was 100.0 μg mL^-1, the median effective concentration （EC50） was 0.46 μg mL^-1, and the selectivity index （TI = TC50/EC50） was 217. LS could inhibit PRV in PK-15 cells when it was added to cell culture medium at 0, 2, 4, 6, and 8 h after PK-15 cells had been infected by PRV. From the results, we concluded that LS had broad antimicrobial spectrum and good inhibiting effects on PRV,  相似文献   

实时荧光定量PCR检测血液标本中光滑念珠菌方法的建立     

孔丹莉  周浩  王茜  艾云灿  何玉清  刘伟伟  杨杰  丁元林 《湛江医学院学报》2013,(5):493-495

目的 探索快速可靠检测血液标本中光滑念珠菌的新方法.方法 采用真菌26S rDNA D1/D2区序列作为引物,单独和同时扩增光滑念珠菌及白色念珠菌与光滑念珠菌混合物,对血液标本进行荧光定量检测.结果 本方法不仅能够稳定的扩增出光滑念珠菌26S rDNA序列,并且能准确区分出混合物中的白色念珠菌与光滑念珠菌.结论 应用26SrDNA D1/D2区序列的荧光定量PCR是快速、敏感和特异的检测光滑念珠菌方法.  相似文献   

肉桂醛、柠檬醛对犬白色念珠菌黏附力及超微结构的影响     

孙博  张志宏  金艺鹏  林德贵 《中国兽医学报》2010,30(2)

根据先前的研究,肉桂醛和柠檬醛都有较好的抗真菌活性。本研究对肉桂醛、柠檬醛在真菌黏附口腔黏膜上皮以及对犬白色念珠菌超微结构的影响进行探索。结果表明,药物处理组白色念珠菌黏附率显著下降。扫描电镜显示,经过药物处理的犬白色念珠菌细胞表面粗糙、皱缩、萎陷、外膜空洞、胞浆内容物流出。透射电镜下,细胞普遍发生细胞壁增厚,质壁分离,萎陷,胞浆内容物外流等损伤。  相似文献   

Influence of diet on the shedding of Candida glabrata by experimentally infected preweaned calves     

Elad D  Brenner J  Markovitcs A  Shlomovitz S  Basan J  Schwartz N 《Veterinary journal (London, England : 1997)》2002,164(3):98-279

The influence of three diets, comprising dam's milk (DM) from the same farm, commercial milk replacer with (CMRL) or without 3.2% lactose (CMR), on the duration and intensity of Candida glabrata shedding in the faeces of preweaned calves following experimental oral infection was examined. Shedding of other potential enteric pathogens was also monitored. The duration and intensity of C. glabrata shedding in DM-fed calves were reduced significantly compared with the calves fed the two diets based on milk replacers. Consequently, feeding calves with DM might disrupt the infective cycle, resulting in the yeast's elimination from a farm. In the CMR and CMRL groups, the periods of intensive shedding of C. glabrata and rotavirus overlapped but no diarrhoea was associated with the shedding of either microorganism. There was no evidence that lactose diminished colonization in vivo.  相似文献   

Suvanine Sesterterpenes from a Tropical Sponge Coscinoderma sp. Inhibit Isocitrate Lyase in the Glyoxylate Cycle     

So-Hyoung Lee  Tae Hyung Won  Heegyu Kim  Chan-Hong Ahn  Jongheon Shin  Ki-Bong Oh 《Marine drugs》2014,12(10):5148-5159

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Effect of nutrient compositions in peat hydrolysate on protein and biomass yields of Candida tropicalis     

F. -H. Chang 《Biology and Fertility of Soils》1986,2(4):187-194

Summary Research was conducted to study the effect of nutrient compositions, at different levels, added in peat hydrolysates on biomass yields and protein of Candida tropicalis. Samples of Minnesota sphagnum peat were hydrolyzed by boiling at 100°C with 1-1 H₂SO₄ solutions to produce peat hydrolysates which can support the growth of yeast. Peat hydrolysates were adjusted to pH 5.5 and mixed with mineral nutrients (NH₄NO₃, NaH₂PO₄ + Na₂HPO₄, KCl, and MgSO₄) and glucose. Among the single nutrients tested, glucose produced the most significant enhancement of biomass yields, followed by NH₄NO₃, NaH₂PO₄ + Na₂HPO₄, KCl, and MgSO₄. NH₄NO₃ was found to be the most important single nutrient affecting protein accumulation in yeast cells, followed by glucose > NaH₂PO₄ + Na₂HPO₄ > KCl > MgSO₄. However, protein and biomass production was significantly (P 0.05) greater for combined nutrients than for any single-nutrient enrichment. The level of residual reducing sugars in peat hydrolysate was inversely proportional to cell counts and optical densities. Addition of high concentrations of nutrients delayed the time of reaching to stationary phase in optical density, cell counts, and pH of yeast cultures. In general, as nutrients were increased, significant (P 0.05) increases in productivity and cell density were observed. However, specific growth rate decreased with increasing nutrient concentrations. It was concluded that nutrient amendments containing supplementary sources of C/N/P/K/S = 400:36:3.3:3.8:1 are most suitable for biomass production and the biosynthesis of protein by C. tropicalis.  相似文献