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Shelton GD Liu LA Guo LT Smith GK Christiansen JS Thomas WB Smith MO Kline KL March PA Flegel T Engvall E 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(3):240-244
The most common form of muscular dystrophy in dogs and humans is caused by mutations in the dystrophin gene. The dystrophin gene is located on the X chromosome, and, therefore, disease-causing mutations in dystrophin occur most often in males. Therefore, females with dystrophin deficiency or other forms of muscular dystrophy may be undiagnosed or misdiagnosed. Immunohistochemistry was used to analyze dystrophin and a number of other muscle proteins associated with muscular dystrophy in humans, including sarcoglycans and laminin alpha2, in muscle biopsy specimens from 5 female dogs with pathologic changes consistent with muscular dystrophy. The female dogs were presented with a variety of clinical signs including generalized weakness, muscle wasting, tremors, exercise intolerance, gait abnormalities, and limb deformity. Serum creatine kinase activity was variably high. One dog had no detectable dystrophin in the muscle; another was mosaic, with some fibers normal and others partly dystrophin-deficient. A 3rd dog had normal dystrophin but no detectable laminin alpha2. Two dogs could not be classified. This study demonstrates the occurrence of dystrophin- and laminin alpha2-associated muscular dystrophy and the difficulty in clinical diagnosis of these disorders in female dogs. 相似文献
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Sung-Il Kang Sang-Eun Lee Ji-Yeon Kim Kichan Lee Jong-Wan Kim Hyang-Keun Lee So-Ra Sung Young-Ran Heo Suk Chan Jung Moon Her 《Comparative immunology, microbiology and infectious diseases》2014
Brucellosis is a zoonotic disease that is transmitted from animals to humans, and the development of a rapid, accurate, and widely available identification method is essential for diagnosing this disease. In this study, we developed a new Brucella canis species-specific (BcSS) PCR assay and evaluated its specificity and sensitivity. A specific PCR primer set was designed based on the BCAN_B0548-0549 region in chromosome II of B. canis. The PCR detection for B. canis included amplification of a 300-bp product that is, not found on other Brucella species or, genetically or serologically related bacteria. The detection limit of BcSS-PCR assay was 6 pg/μl by DNA dilution, or 3 × 103 colony-forming units (CFU) in the buffy coats separated from whole blood experimentally inoculated with B. canis. Using the buffy coat in this PCR assay resulted in approximately 100-times higher sensitivity for B. canis as compared to detect directly from whole blood. This is the first report of a species-specific PCR assay to detect B. canis, and the new assay will provide a valuable tool for the diagnosis of B. canis infection. 相似文献
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Alexandra Pantchev Reinhard Sting Rolf Bauerfeind Judith Tyczka Konrad Sachse 《Comparative immunology, microbiology and infectious diseases》2010,33(6):473-484
The aim of the present study was to analyse the occurrence of chlamydiae in several mammalian host species. Clinical samples that previously tested positive in a Chlamydiaceae-specific real-time PCR were retested using six species-specific real-time PCR assays to identify the chlamydial species involved. Chlamydophila (Cp.) abortus was the agent most frequently found in cattle, sheep, horses, goats, and pigs. Detection in cattle of Cp. psittaci (11% of samples) and Chlamydia (C.) suis (9%), as well as Cp. psittaci in a goat sample was somewhat unexpected. DNA of two different chlamydiae was identified in 56 (12.7%) of 440 samples tested. Cp. felis was the predominant species found in cats, while in guinea pigs and rabbits only Cp. caviae was detected. Interestingly, the latter two pathogens were also identified in samples from dogs. The data show that mixed chlamydial infections are not rare and suggest an extended host range of individual species. 相似文献
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Orsolya Kutasi Nandor Balogh Zoltan Lajos Krisztina Nagy Otto Szenci 《Journal of Equine Veterinary Science》2011,31(7):400-410
Even though the respiratory system is one of the most accessible organs for diagnostic testing, it is not always easy to define chronic lower airway disease in the horse. Diagnostic procedures performed by first opinion veterinarians in the field are often restricted to taking the history and performing clinical examination. Respiratory tract endoscopy, tracheal or bronchoalveolar lavage, and blood sampling are sometimes used but other specific ancillary examinations are seldom performed in stable settings. Therefore, our objectives were to evaluate the diagnostic value of different techniques and examination types routinely used in the diagnostic workup of chronic equine lower airway cases in both stable and clinical circumstances. Another aim of this study was to estimate the prevalence of different chronic pulmonary disorders among horses admitted to a Hungarian referral clinic. According to the conditional inference tree method, age of the horse, history, clinical examination, respiratory tract endoscopy, and bronchoalveolar lavage cytology proved to be the most valuable tools to define pathology. It was also concluded that in 22% of cases, more specific ancillary diagnostic modalities, unavailable for the field veterinarian, were needed to establish the final diagnosis. According to our study, the most frequently diagnosed chronic pulmonary disorders in Hungary are of noninfectious origin, principally recurrent airway obstruction. Regardless of the cause, and interestingly including recurrent airway obstruction as well, these diseases occur primarily during the warm months. 相似文献
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