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F107(F18ab)菌毛单抗的制备、鉴定与初步应用 总被引：9，自引：2，他引：7
将大肠杆菌107／86和水肿病菌株在TSB中培养，鉴定其表达F107菌毛，前者作为免疫原免疫BABL／C小鼠，后者作为检测抗原包装酶标板。细胞融合后经ELISA和玻板凝集检测，得到一株阳性杂交瘤细胞株，命名为F7－1。该细胞株制备的腹水ELISA效价为：2^16，试管凝集价为：1：5120，在免疫印迹中可与提取的F107菌毛特异性条带发生反应。利用此单抗检测表明，91％的水肿病菌株表达F107菌毛。 相似文献
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为探索更为有效的黏附素提取技术,本试验以猪源ETEC F18ab菌株为材料,通过三种方法（切割法、热洗脱法及联合热洗脱与切割法）各提取200mL菌悬液中的黏附素,应用紫外可见分光光度计测定浓度并进行SDS—PAGE分析。结果显示,应用切割法、热洗脱法和联合热洗脱与切割法所得黏附素量分别为5．94、8．84和22．47mg,即应用联合热洗脱与切割法提取的黏附素量明显高于单纯应用热洗脱法和切割法;SDS—PAGE分析表明,联合热洗脱与切割法提取的黏附素蛋白纯度稍低于单纯应用热洗脱法和切割法。综合分析上述结果可知,联合热洗脱与切割法更适用于大量黏附素抗原的提取。 相似文献
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H. Imberechts H.U. Bertschinger M. Stamm T. Sydler P. Pohl H. De Greve J.-P. Hernalsteens M. Van Montagu P. Lintermans 《Veterinary microbiology》1994,40(3-4):219-230
The study comprises fifty 4 to 12 weeks old pigs that died from oedema disease or severe diarrhoea. Smears were prepared from the mucosa of duodenum, jejunum and ileum, and by immunofluorescence F107 fimbrial antigens were detected. E. coli. strains were isolated from the intestines and were characterised by slide agglutination (serogroup and F107 fimbriae production), by their cytotoxicity for Vero cells, and by gene amplification (genes coding for the major F107 subunit FedA, the toxin causing oedema disease SLT-IIv, and enterotoxins LTI, STIa and STII). F107 fimbriae were demonstrated in association with E. coli of serogroups O139:K12 and O141:K85a,b but not of serogroup O149:K91:F4a,c. Expression in culture of F107 fimbriae by some isolates gave additional evidence for production of these fimbriae by ETEC strains. The genetic determinant of SLT-IIv was found in association with F107, and could not be detected in serogroup O149:K91:F4a,c. Gene fedA was demonstrated in two isolates which were devoid of SLT-IIv. Most isolates from cases of oedema disease belonged to serogroup O139:K12 and did not contain enterotoxin genes. Isolates from pigs that suffered from diarrhoea were serotyped O141:K85a,b or O149:K91:F4a,c, and carried at least two enterotoxin genes in their genomes. In a small proportion of the cases F107 antigens were demonstrated in intestinal smears although gene fedA was not detected in the corresponding isolates. The results confirm the importance of F107 fimbriae as virulence factor in oedema disease E coli strains, but also demonstrate that F107 fimbriae can be found in association with postweaning diarrhoea isolates. In these latter strauns enterotoxins were always demonstrated, irrespective of the presence of toxin SLT-IIv. 相似文献
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