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AIM: To investigate the effect of cyclopamine, a Hedgehog(Hh) signaling pathway inhibitor, on the biological behavior of intrahepatic cholangiocarcinoma cell line RBE. METHODS: The proliferation of RBE cells was detected by cell counting with Typan blue staining and MTT assay, and the apoptosis was analyzed by the flow cytometry. The Transwell invasive cabin assay was used to detect the invasion ability, and Western blot was used to determine the protein expression of Gli1 and MMP-9 in the RBE cells before and after cyclopamine treatment. RESULTS: Cyclopamine inhibited the growth of RBE cells in a time-and dose-dependent manner. After cyclopamine treatment for 24 h, 48 h and 72 h, the apoptotic rates were significantly higher than those in control group. In control group, the number of cells invading through the Matrigel of invasion chamber was 154.52±13.61, while in experimental group it was 62.00±12.17, indicating that the invasion ability of the cells declined significantly. Furthermore, Western blot showed that the protein levels of Glil and MMP-9 in the RBE cells were decreased after treatment with cyclopamine for 24 h and 48 h. CONCLUSION: Blockage of the Hh signaling pathway with cyclopamine suppresses the proliferation, promotes the apoptosis and inhibits the invasion ability of RBE cells. 相似文献
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猴头菇制剂生物学效应观察 总被引：3，自引：0，他引：3
猴头菇制剂是以猴头菇子实体加水（１：２０）浸提，再经酵母发酵而制得。动物实验结果表明，对兔离体肠管运动具有明显的加快加强作用，并能明显的扩张蟾蜍小动脉血管，具有改善微循环的作用。 相似文献
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AIM: To investigate the effect of Sonic Hedgehog (Shh) signaling blockade on the growth of hematocarcinoma cells and underlying mechanisms. METHODS: The expression of Shh signaling molecules in hematocarcinoma cell lines BEL-7402, Huh7 and HepG2 was detected by RT-PCR. The cell viability was detected by MTT assay. The cell cycle and apoptosis were analyzed by flow cytometry. The expression of apoptosis-related proteins was determined by Western blot. RESULTS: Shh signaling molecules were all expressed in BEL-7402, Huh7 and HepG2 cells. The mRNA expression of Patched (Ptch), Gli1 and Gli2 was down-regulated by anti-Shh antibody. Blockade of Shh signaling pathway inhibited the proliferation of hepatocarcinoma cells with increasing cells in G0/G1 phase and induced the apoptosis of hepatocarcinoma cells. Treatment with anti-Shh antibody down-regulated the protein expression of pro-caspase-3, pro-caspase-8 and pro-caspase-9, while up-regulated the protein levels of cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 in BEL-7402 cells. CONCLUSION: Blockade of Shh signaling pathway inhibits the growth of hepatocarcinoma at different levels by cell cycle arrest and inducing apoptosis of hematocarcinoma cells. 相似文献
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AIM: To investigate the effects of curcumin on pancreatic cancer cells and the possible molecular mechanisms. METHODS: The pancreatic cancer PANC-1 cells were treated with curcumin. Growth inhibitory rate of the cells was measured by MTT assay. Apoptosis of the cells was detected by flow cytometry. The expression levels of K-Ras,extracellular signal-regulated kinase 1/2(ERK1/2),phosphorylated ERK1/2(p-ERK1/2),Sonic hedgehog (Shh) and glioma-associated oncogene homolog 1(GLI1) were determined by Western blotting. RESULTS: The growth inhibitory rate of the cells in 30 mmol/L curcumin group was significantly different from that in other groups. Apoptotic rate in 30 mmol/L curcumin group (37.57%) was significantly higher than that in control group (4.62%). The expression levels of K-Ras, p-ERK1/2, Shh and GLI1 in curcumin groups were significantly lower than those in control group. CONCLUSION: Curcumin inhibits proliferation and induces apoptosis of pancreatic cancer cells by inhibiting Ras-ERK and Shh-GLI1 signal pathways. 相似文献
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JIN Hai LIU Hui-ling LI Xue-jun FENG Zhi-ying WANG Bing YANG Jian-xu WANG Gen-shu WU Bin 《园艺学报》2012,(1):90-93
AIM: To detect the expression of Hedgehog signaling molecules in rat livers after partial hepatectomy. METHODS: The model of rat partial hepatectomy was established by resecting the middle and left lobes of the liver. Eighteen male Sprague-Dawley rats were divided into 3 groups: sham operation group (group A), partial hepatectomy group 1 (group B) and partial hepatectomy group 2 (group C). Hepatic tissues were collected 24 h after the operation in group A and group B, and 48 h after the operation in group C. The expression of Ki-67,Sonic Hedgehog(Shh),Indian Hedgehog(Ihh) and Glioblastoma-2(Gli-2) in the hepatic tissues were detected by immunohistochemical staining and Western blotting. RESULTS: The edema and spotty necrosis in the hepatic tissues were observed in group B and group C by HE staining. The cells of different dividing stages were found in the hepatic tissues of group C. Compared with group A, the expression of Ki-67, Shh, Ihh and Gli-2 in group B (P<0.01) and group C (P<0.01) was significantly elevated, and the expression levels in group C were higher than those in group B (P<0.01). CONCLUSION: Hedgehog signaling in rat livers may be activated after partial hepatectomy and stimulate liver regeneration. 相似文献
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刺猬肌肉、脂肪品质及营养成分分析 总被引：1，自引：0，他引：1
对野生动物刺猬（Ｅｒｉｎａｃｅｕｓｅｕｒｏｐａｅｕｓ）肌肉、脂肪的品质及营养成分进行器官及理化检验。结果表明,刺猬肌肉含蛋白质２０２％,脂肪８％,矿物质１％,熟肉率６１２％,肌纤维直径１８９５±３４５μｍ,脂肪熔点２０℃～３０℃,碘价９４～９９,皂化价１７０～１７５。肌肉的品质及营养成分可与常用动物肉（猪、牛、羊、鸡、兔肉）相媲美;油脂与常用动植物油脂相比,感官指标良好,脂肪酸不饱和程度明显高于动物油脂,而与花生油等多种植物的不饱和程度相当。 相似文献