The expression of IFN-γ and IL-4 on T lymphocytes that infiltrate in nasal polyps     

CHEN Rong  SHI Jian-bo  XU Geng  DI Jing-fang  ZENG Shan  ZENG Yao-ying 《园艺学报》2003,19(12):1623-1626

AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P＜0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps.  相似文献   

Expression of vascular endothelial growth factor in chronic inflammatory mucosa of lacrimal sac     

ZHANG Tao  WANG Ji-qun  WANG Li-hua  SHAN Yan-chun 《园艺学报》2004,20(3):459-461

AIM: To study the expression of vascular endothelial growth factor (VEGF) in inflammatory mucosa of lacrimal sac. METHODS: Immunohistochemical S-P method was used to examine the expression of VEGF in the mocusa from 12 patients with chronic dacryocystitis and 8 volunteers. RESULTS: The positive rates of VEGF expression in different parts of the mocusa were: basal lamina: 44.3%±7.6％; surface epithelium: 16.9%±4.6％; connective tissue: 15.2%±4.9％, all normal mocusa of 8 cases were negative. There was a significant difference between the two groups (P<0.01), a significant difference among each part of the chronic inflammatory mocusa of lacrimal sac. CONCLUSION: VEGF may play an important role in hyperplasia of inflammatory mucosa of lacrimal sac.  相似文献   

Effect of forsythiaside A on immune function in rats with ulcerative colitis     

YU Liang  JIANG Hui-li  LIU Hao-jie 《园艺学报》2020,36(6):1128-1132

AIM To investigate the effect of forsythiaside A (FA) on immune function in rats with ulcerative colitis and its related mechanism. METHODS Healthy SD rats were randomly divided into 5 groups: control group (no treatment, normal feeding), model group (establishment of rat ulcerative colitis model), and low, medium and high doses of FA groups (treatment of the model rats with FA at 5 mg/kg, 20 mg/kg and 80 mg/kg, respectively). The malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in rat colon tissues were measured by colorimetry, and the serum levels of tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2) and IL-4 were detected by ELISA. The spleen index and thymus index, the percentages of CD3⁺, CD4⁺ and CD8⁺ T-lymphocytes in peripheral blood mononuclear cells （PBMC）, the serum IgA and IgG levels, and the serum complement C3 and C4 levels were also determined. RESULTS The colon tissues of the rats in model group showed obvious inflammation and ulceration, indicating that the animal model was successfully established. Compared with model group, the colonic inflammation and ulceration were significantly attenuated in FA groups, among which the high dose had the best effect. Compared with control group, the spleen index and thymus index of the rars in model group were decreased (P<0.05), MDA content in colon tissues was increased (P<0.05), and SOD activity in colon tissues was decreased (P<0.05). The levels of CD3⁺, CD4⁺, CD8⁺ and CD4⁺/CD8⁺ T-lymphocytes in PBMC, and the serum levels of C3, C4 and IL-4 were decreased (P<0.05), while the serum levels of IgA, IgG, TNF-α, and IL-2 were increased in model group as compared with control group. Furthermore, the spleen index and thymus index of the rats in FA groups were increased (P<0.05), the MDA content in the colon tissues was decreased (P<0.05), and the SOD activity in the colon tissues was increased (P<0.05). The levels of CD3⁺, CD4⁺, CD8⁺ and CD4⁺/CD8⁺ T-lymphocytes in PBMC, and the serum levels of C3, C4 and IL-4 were increased (P<0.05), while serum IgA, IgG, TNF-α and IL-2 levels were decreased in FA groups as compared with model group (P<0.05). CONCLUSION Forsythiaside A effectively attenuates the colonic lesions in rats with ulcerative colitis, and its mechanism may be related to reinforcement of oxygen free radical scavenging power, alleviation of inflammatory response, and enhancement of immune function.  相似文献   

大肠癌p53的表达与病理、PCNA、CEA及p53、PCNA、CEA与淋巴结转移的关系   总被引：2，自引：0，他引：2  

许庆文  李岩松  朱荷根  黄应桂 《湛江医学院学报》1997,(3)

目的：研究大肠癌p53的表达与病理、增殖细胞核抗原（PCNA）、癌胚抗原（CEA）及p53、PC-NA、CEA与淋巴结转移的关系。方法：应用链霉菌素-生物素（SP）免疫组化法，观察44例经病理确诊的大肠癌石腊标本中的p53表达、PCNA的阳性率和CEA的表达型式。结果：大肠癌p53阳性表达率为52．3％；大肠癌p53阳性表达与性别、年龄及肿瘤的部位、分化程度和湿润深度无关（P＞0．05），p53阳性表达者其淋巴结转移率较阴性者高（P＜0．05）；p53阳性表达及有淋巴结转移者其细胞增殖活性分别较p53阴性表达及无淋巴结转移者高（P＜0．05）；p53阳性表达及有淋巴结转移者其CEA表型均以胞浆型和间质型为主（P＜0．05）。结论：检测p53和PCNA表达及CEA表型对判断大肠癌的恶性程度、预测其林巴结转移趋势和预后及指导临床治疗有重要价值。  相似文献   

Captopril exerts myocardial protective effect in coronary microembolization rats by inhibiting oxidative stress injury and alleviating inflammatory response     

HUO Yan-ping  JIAO An-de  LIU Yu-mei  ZHANG Xiao-li 《园艺学报》2019,35(9):1614-1619

AIM: To investigate the effects of captopril (CAP) on oxidative stress injury and inflammatory response induced by coronary microembolization (CME) and its related molecular mechanisms. METHODS: The rat model of CME was established by clamping the rat artery and injecting blood microemboli. The rats were divided into control group, CME group and CME+CAP group, with 6 rats in each group. The myocardial tissues of each group were collected. The changes of myocardial structure and the degree of inflammatory response were analyzed by HE staining. Cardiomyocyte apoptosis was detected by TUNEL staining. The fluorescence intensity of cleaved caspase-3 was detected by immunofluorescence obervation. The protein levels of cleaved caspase-3 and Bax were determined by Western blot. The activity of superoxide dismutase (SOD) and catalase was measured by ELISA. The production of reactive oxygen species (ROS) was detected by DHE fluorescence staining. RESULTS: CAP significantly reduced the myocardial structural changes (P<0.05), inflammatory cell infiltration (P<0.01), number of apoptotic cardiomyocytes (P<0.01), the protein levels of cleaved caspase-3 and Bax (P<0.01), and ROS production levels (P<0.01), but promoted the activity of antioxidant markers SOD and catalase (P<0.01) in the CME rats.CONCLUSION: CAP attenuates CME-induced myocardial injury by resisting oxidative stress and alleviating inflammatory response.  相似文献   

Suramin alleviates hypertrophic scar in mice by inhibiting fibroplasia and inflammatory response     

LIN Kang  SU Hai-yan  YU Qing  JIANG Liang-fu  GAO Wei-yang 《园艺学报》2019,35(11):2070-2077

AIM: To investigate the therapeutical effect of suramin on hypertrophic scar (HPS) and its mechanism. METHODS: After the mouse model of HPS was established by mechanical stretching, the suramin solution at low dose (5 mg/kg) and high dose (10 mg/kg) was applied onto the scar site caused by mechanical load in mice by transdermal administration once a day for 10 d. The degree of scar hyperplasia was observed by macroscopy. The scar cross-sectional area and scar elevation index in the HPS tissues were evaluated by hematoxylin-eosin (HE) staining. The expression levels of transforming growth factor-β1 (TGF-β1) and interleukin-6 (IL-6) in HPS tissues were detected by immumohistochemical staining. The expression level of α-smooth muscle actin (α-SMA) in HPS tissues was detected by immunofluorescence staining, RT-qPCR and Western blot. The levels of tumor necrosis factor-α (TNF-α), IL-6, IL-10 and TGF-β1 in the HPS tissues were measured by ELISA. RESULTS: Macroscopic observation showed that the surface areas of scar in the HPS mice after treatment with suramin at low and high doses were significantly reduced (P<0.01). HE staining results showed that the scar cross-sectional area and the scar elevation index of HPS mice after treatment with suramin at low and high doses were significantly reduced (P<0.05 or P<0.01). The results of immunofluorescence staining, RT-qPCR and Western blot showed that the number of α-SMA positive cells and the mRNA and protein expression of α-SMA in scar tissues of HPS mice after treatment with suramin at low and high doses were significantly decreased (P<0.05 or P<0.01). The results of immunohistochemical staining showed that the expression levels of TGF-β1 and IL-6 in scar tissues of HPS mice after treatment with suramin at low and high doses were significantly reduced (P<0.01). The results of ELISA showed that the levels of TNF-α, IL-6, IL-10 and TGF-β1 in the scar tissues of HPS mice after treatment with suramin at low and high doses were significantly reduced (P<0.01). CONCLUSION: Suramin inhibits the formation of HPS, which may be related to the inhibition of fibroplasia and reduction of local inflammatory response.  相似文献   

Role of TLR4 in process of colonic inflammation recovery induced by LPS     

LI Wang-lin  LIU Meng-ao  CAO Jie  YANG Ping  ZHENG Xiao-bin  DONG Bo-ye  LU Jia-bao 《园艺学报》2017,33(2):336-343

  相似文献   

益生菌在治疗炎症性肠病方面的应用     

朱军伟  杭锋  王钦博等 《安徽农业科学》2014,(1):124-127

从炎症性肠疾病（IBDs）的发病原因入手,重点阐述了益生菌在调节肠道菌群平衡、维护肠粘膜屏膜功能以及调节肠相关免疫体系平衡缓解和治疗炎症性肠疾病方面的作用机理。  相似文献   

Effects of Huaiyu decoctum on serum concentrations of TNF-α, IL-6 and IL-10 in rats after anorectal operation     

LIU Yu-yan  XU Xiang-qian  LUO Wen-zhao 《园艺学报》2016,32(11):2091-2094

ATM: To investigate the effects of Huaiyu decoctum on the serum concentrations of TNF-α, IL-6 and IL-10 in rats after anorectal operation. METHODS: Sprague-Dawley rats (n=40) were randomly divided into 4 groups:normal group, model group, low-dose Huaiyu decoctum group and high-dose Huaiyu decoctum group. The concentrations of TNF-α, IL-6 and IL-10 in the rat serum were measured by ELISA. The pathologic changes of the anorectal tissues were observed under microscope with HE staining. The protein expression of ICAM-1, VCAM-1 and NF-κB was determined by Western blotting. RESULTS: After Huaiyu decoctum administration, TNF-α and IL-6 concentrations in the serum were significantly decreased, and IL-10 concentration was increased as compared with model group. Moreover, Huaiyu decoctum markedly attenuated edema and hyperemia in the rats after anorectal operation. The protein expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and NF-κB in the anorectal tissues was obviously inhibited by Huaiyu decoctum treatment. CONCLUSION: Huaiyu decoctum improves the recovery of anorectal tissues after operation by decreasing the serum concentrations of TNF-α, IL-6 and IL-10, and reducing the protein expression of ICAM-1, VCAM-1 and NF-κB in the anorectal tissues.  相似文献   

恰玛古胶囊联合FOLFIRI方案对晚期大肠癌患者免疫功能的临床观察   总被引：1，自引：0，他引：1  

罗玲娟  黄建国  雷君  张立平  何文婷  谢新梅  曾凡业  节阳华  帕提玛·阿布力米提  樊玉祥  张洪亮 《人参研究》2013,(4):56-59

目的 探讨恰玛古胶囊对使用FOLFIRI方案的大肠癌患者免疫功能影响.方法 将符合条件的64例大肠癌患者,随机分为对照组,实验组,两组均给予一周期FOLFIRI标准化疗,实验组加服恰玛古胶囊15粒一日三次,连服14天.对照组给予安慰剂胶囊15粒一日三次,连服14天.治疗前后分别进行KPS评分,血清中淋巴细胞亚群及白介素-15测定.结果共观察61例,治疗组31例,对照组30例.治疗组：KPS评分比较,实验组显著优于对照组.两组治疗后治疗组CD3＋、CD4＋、CD4＋/CD8＋比值高于对照组,CD8＋低于对照组,两组治疗前后IL-15血清浓度无统计学差异.结论 恰玛古胶囊可提高FOLFIRI患者的KPS评分,改善其体力状态,对其免疫系统有正向调节作用.  相似文献