排序方式： 共有5条查询结果，搜索用时 0 毫秒
1
1.
河南省兔球虫感染情况及种类调查研究 总被引：3，自引：3，他引：0
为了掌握河南省兔球虫感染情况,用麦克马斯特虫卵计数法和饱和氯化钠溶液漂浮法对来自河南省不同地区的11个兔场共465份新鲜粪便进行兔球虫感染情况调查和球虫种类形态鉴定。结果发现：兔球虫平均感染率为74.6%,多数为混合感染,感染球虫种类多在4~6种,以1—3月龄幼兔感染率最高,达83.0%,并且感染强度OPG值最高,平均为6.8×104。本次共检出11种艾美耳球虫,其中大型艾美耳球虫、无残艾美耳球虫、穿孔艾美耳球虫、肠艾美耳球虫为优势种。本实验结果为进一步有效控制兔球虫病和筛选兔球虫疫苗提供重要的参考依据。 相似文献
2.
Helena Carstensen Lene Larsen Christian Ritz Martin K. Nielsen 《Journal of Equine Veterinary Science》2013
Strongyle parasites are ubiquitous in grazing horses and constitute a potential threat to equine health. Feces were collected from six horses four times daily over a period of 5 days. Fecal egg counts (FECs) were performed to identify any diurnal rhythms in strongyle egg shedding and to quantify variability at the different levels: individual horses, repeated counts, repeated subsamples, different time points, and different days. No significant differences in FECs were found between the different time points (P = .11). The variables—horse, day, subsample, and egg count—accounted for a variance of 104.83, 0.10, 7.24, and 5.61, respectively. The apparent lack of additional variability between the four different time points suggests that time of the day chosen for collecting fecal samples does not constitute a source of error in field studies. The majority of variability exists between different subsamples and repeated egg counts on the same subsamples, whereas the variability of FECs between following days can be considered negligible. The findings of this study have implication for designing and performing field surveillance of strongyle FEC levels and applying the FEC reduction test for evaluating anthelmintic efficacy. 相似文献
3.
为了评价一种改进型麦克马斯特虫卵计数板的性能,利用改进型麦克马斯特虫卵计数板和经典麦克马斯特虫卵计数板对兔粪便样品和球虫卵囊悬液样品进行计数和比较分析。结果显示,用饱和氯化钠溶液作为漂浮液,改进型麦克马斯特虫卵计数板能对卵囊悬液样品和2~5 g固体粪便样品中球虫卵囊进行准确计数,与麦克马斯特虫卵计数板计数结果的符合率为95.59%~99.49%;用自来水作为悬浮液,改进型麦克马斯特虫卵计数板能对卵囊悬液样品和0.3~0.5 g固体粪便样品中球虫卵囊进行准确计数。本研究发现,改进型麦克马斯特虫卵计数板对临床和实验室样品中虫卵能进行准确计数,而且比经典麦克马斯特虫卵计数板具有更强大的检测功能。 相似文献
4.
The use of fecal egg count techniques to indirectly assess intestinal parasite burdens and determine anthelmintic efficacy is common in parasitological research and veterinary practice. The McMaster method is one of the most widely used techniques in veterinary practice, but recently, the Mini-FLOTAC technique has been introduced as a possible alternative. Studies comparing the two methods in precision and accuracy are needed. This study aimed at evaluating the Mini-FLOTAC technique for determining equine strongyle egg counts through a two-part procedure. First, a set of fecal counts was executed using both methods. Next, blind counts were performed on spiked fecal samples with true egg counts at 0, 5, 50, 500, and 1,000 eggs per gram. All counts were performed in triplicates, and each sample was counted using both methods. Mini-FLOTAC and McMaster had 83.2% and 53.7% precision, respectively. The accuracy was found to be 42.6% and 23.5% for Mini-FLOTAC and McMaster, respectively. In conclusion, this study found that Mini-FLOTAC exhibited both higher precision and accuracy than the McMaster technique and appears to be a more reliable alternative. Using a more precise egg-counting method can help assure that changes in egg counts before and after treatment reflect a genuine reduction and are not due to chance variability. 相似文献
5.
4种粪便检查方法在球虫病临床检测中的应用效果评价 总被引：1，自引：1，他引：0
为了评价不同粪便检查方法在球虫病预防性检测和临床病例诊断中的应用效果,本研究通过在无球虫鸡新鲜粪便中添加不同浓度的柔嫩艾美耳球虫孢子化卵囊模拟临床样品,共8个梯度,然后用改进型直接涂片法、水洗沉淀法、饱和氯化钠溶液漂浮法和改进型麦克马斯特虫卵计数法进行检测和计数。结果显示,改进型直接涂片法检测下限为6000个/g,水洗沉淀法检测下限为3000个/g,这两种方法镜下检测均有大量杂质。饱和氯化钠溶液漂浮法检测下限为100个/g,改进型麦克马斯特虫卵计数法检测下限为300个/g,二者镜下视野中杂质含量均较少。添加回收试验结果显示,改进型麦克马斯特虫卵计数法平均回收率为82.2%,平均变异系数为3.7%;3~5 g为最佳检测粪样重量,具有较高的准确性和可重复性。综合考虑,改进型麦克马斯特虫卵计数法是一种理想可靠的球虫病临床检测方法,在集约化养殖场畜禽球虫病防控中具有重要的推广价值。 相似文献
1