Oral malignant melanoma – the effect of coarse fractionation radiotherapy alone or with adjuvant carboplatin therapy     

S. Murphy  A. M. Hayes  L. Blackwood  G. Maglennon  H. Pattinson  A. H. Sparkes 《Veterinary and comparative oncology》2005,3(4):222-229

A retrospective study was undertaken of dogs presented to the Animal Health Trust for treatment of oral malignant melanoma, without radiographic evidence of pulmonary metastases. Group 1 (n = 13) received radiotherapy of the primary and any lymph node metastases (4 weekly fractions of 9 Gy); and group 2 (n = 15) were treated the same but additionally received between two and six doses carboplatin at 300 mg m^?2 every 3 weeks. Median survival times for the two groups were 307 and 286 days, respectively (P > 0.05). In addition, carboplatin therapy did not significantly reduce the proportion of dogs dying due to metastases (three from group 1 and four from group 2). We found no evidence of a beneficial effect of carboplatin therapy over radiotherapy alone.  相似文献   

1例肉鸡肝恶性黑色素瘤的病理学诊断     

杨腊梅  张婷  张鼎 《畜牧兽医学报》2021,52(1):268-272

本研究旨在对临床上发现的1例肉鸡肝黑色素瘤进行病理学诊断。1羽25日龄AA肉鸡精神沉郁、极度消瘦,剖检发现其整个肝呈黑色。HE染色结果显示,病变肝内遍布大小不一、细胞核深染、细胞质内有大量黑色素颗粒沉积的异常细胞团。黑色素瘤诊断标志蛋白SOX 10和HMB-45抗体免疫组化染色均呈阳性。结合病理学检测和免疫组化结果,判定该病鸡肝患有恶性黑色素瘤。试验对病变肝进行胆色素染色和普鲁士蓝染色,结果均呈阴性,提示病变肝并非由胆红素或含铁血黄素淤积所致。综上表明,此例病鸡患有严重的肝恶性黑色素瘤,试验结果对禽类黑色素瘤的诊断与研究有参考价值。  相似文献   

基于改进遗传脉冲耦合神经网络的玉米病害图像分割方法   总被引：3，自引：0，他引：3  

徐亚静  温长吉  王增辉 《吉林农业大学学报》2013,35(4)

作物病害图像分割是利用数字图像处理技术进行病害识别的关键性技术环节之一,现有病害分割方法存在病害区域外部形态特征细节保留程度差和颜色纹理信息丢失等问题.针对上述问题,提出一种基于改进遗传算法的脉冲耦合神经网络分割方法.首先改进遗传算法,以信息熵的加权线性组合作为优化适应度函数用以在每次迭代过程中评价脉冲耦合神经网络对于病害区域的分割效果,通过计算种群适应度方差和适应度均值自适应调整遗传算法的交叉概率和变异概率;然后将改进遗传算法与脉冲耦合神经网络相结合,实现网络连接系数、衰减系数和幅值系数的自动优化调节;最后利用改进遗传脉冲耦合神经网络分割算法,在RGB子空间分别对病害图像进行病害区域分割,将分割结果利用RGB颜色空间子图合并策略实施合并,从而得到最终的图像分割结果.将此算法、最小交叉熵阈值化算法和GA-PCNN算法用于玉米黑瘤粉病彩色图像病害区域分割.结果表明:从主观视觉评测角度,此算法分割效果较好,能够较为细致的呈现病害区域的外部形态特征和较为完好的保留病害区域的颜色纹理特征;从客观量化评测角度,此分割算法在目标区域分割匹配率、错分率和正确率上明显优于最小交叉熵阈值化算法和GA-PCNN算法.  相似文献   

甲硫基腺苷对小鼠黑色素瘤B16F10细胞系的抑制作用     

李明堂  王清爽  冀伟 《吉林农业大学学报》2012,(3):321-323,338

应用细胞培养技术研究了甲硫基腺苷对小鼠黑色素瘤B16F10细胞系体外增殖和体内增长的抑制作用。结果表明:甲硫基腺苷抑制了小鼠黑色素瘤B16F10细胞系的体外增殖(IC50为10μmol/L,P<0.01)和体内增长(抑瘤率为30.8%,P<0.05)。  相似文献   

Tumours of the vulva and vagina in cattle--a 10-year survey     

Yeruham I  Perl S  Orgad U  Yakobson B 《Veterinary journal (London, England : 1997)》1999,158(3):325-239

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Canine choroidal melanoma with metastases     

Hyman JA  Koch SA  Wilcock BP 《Veterinary ophthalmology》2002,5(2):113-117

A 3-year-old-female, spayed Golden Retriever was examined for a unilateral retinal detachment with exophthalmos. Ultrasonographically, a mass was detected with intra- and extraocular extension. The orbit was exenterated and the dog recovered uneventfully. Histopathologic diagnosis was a primary choroidal melanoma with orbital extension, however, the behavioral and cytologic features were benign. Routine examinations postsurgically were nonremarkable. Twenty-one months after surgery the dog was euthanized for respiratory collapse with radiographic signs of metastasis. Necropsy revealed black lesions in the lung and liver. Histopathologic diagnosis was metastatic melanoma with morphology and behavior identical to the primary choroidal melanoma. This is the first definitive case of a canine choroidal melanoma with metastasis.  相似文献   

Expression of vascular endothelial growth factor in canine oral malignant melanoma     

K. H. Taylor  A. N. Smith  M. Higginbotham  D. D. Schwartz  D. M. Carpenter  E. M. Whitley 《Veterinary and comparative oncology》2007,5(4):208-218

Serum, plasma and tissue expression of vascular endothelial growth factor (VEGF) was measured in 20 dogs previously diagnosed histologically with oral melanoma. The concentrations of VEGF in serum and plasma were significantly higher in dogs with melanoma than in a control population (P ≤ 0.002). Concentrations of VEGF in the serum and plasma of dogs with melanoma were highly correlated (r = 0.867). Ninety‐five per cent of melanoma tissues expressed VEGF. Two staining patterns were detected: diffuse and granular cytoplasmic staining. High blood concentrations of VEGF were correlated to a shorter survival time in dogs receiving definitive therapy (P = 0.002). Survival times were significantly longer in dogs receiving definitive therapy versus palliative therapy (median 496 versus 97 days, P = 0.007). Blood concentrations of VEGF were associated with stage (P < 0.05). Dogs with oral melanoma have increased serum, plasma and tissue concentrations of VEGF. Increased expression of VEGF may be a reasonable target for future therapy of canine oral melanoma.  相似文献   

A matched observational study of survival in cats with enucleation due to diffuse iris melanoma   总被引：2，自引：1，他引：1  

Kalishman JB  Chappell R  Flood LA  Dubielzig RR 《Veterinary ophthalmology》1998,1(1):25-29

Although a small number of cases of feline diffuse iris melanoma have been documented to metastasize, the prognosis is not known. In this matched observational study, the survival time of 34 cats with enucleation due to histologically confirmed diffuse iris melanoma was recorded. These results are compared to the survival times of 83 age-matched control cats. Affected cats had enucleation between 2 and 10 years prior to the study. One group of control cats with eye disease had enucleation for either lymphoplasmacytic uveitis (27 cases), ocular trauma (seven cases), or endophthalmitis (four cases). In these control cats, enucleations were performed between 2 and 10 years prior to this study. Forty-five additional control cats presented for vaccination between 2 and 10 years prior to the study. The extent of diffuse iris melanoma at the time of enucleation in affected cats was graded according to the extent of involvement of ocular tissues and the invasiveness of the tumor. Affected cats have a significantly decreased survival compared with control cats and cats with extensive tumors at the time of enucleation have the lowest survival rates. Cats with tumors confined to the iris survive at the same rate as controls. These results suggests that early enucleation is important to avoid premature death, presumed to be due to cancer metastasis in cats with diffuse iris melanoma.  相似文献   

The therapeutic effects of SET/I2PP2A inhibitors on canine melanoma     

Shuhei ENJOJI  Ryotaro YABE  Nobuyuki FUJIWARA  Shunya TSUJI  Michael P. VITEK  Takuya MIZUNO  Takayuki NAKAGAWA  Tatsuya USUI  Takashi OHAMA  Koichi SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1451-1456

Canine melanoma is one of the most important diseases in small animal medicine. Protein phosphatase 2A (PP2A), a well conserved serine/threonine phosphatase, plays a critical role as a tumor suppressor. SET/I2PP2A is an endogenous inhibitor for PP2A, which directly binds to PP2A and suppresses its phosphatase activity. Elevated SET protein levels have been reported to exacerbate human tumor progression. The role of SET in canine melanoma, however, has not been understood. Here, we investigated the potential therapeutic role for SET inhibitors in canine melanoma. The expression of SET protein was observed in 6 canine melanoma cell lines. We used CMeC-1 cells (primary origin) and CMeC-2 cells (metastatic origin) to generate cell lines stably expressing SET-targeting shRNAs. Knockdown of SET expression in CMeC-2, but not in CMeC-1, leads to decreased cell proliferation, invasion and colony formation. Phosphorylation level of p70 S6 kinase was decreased by SET knockdown in CMeC-2, suggesting the involvement of mTOR (mammalian target of rapamycin)/p70 S6 kinase signaling. The SET inhibitors, OP449 and FTY720, more effectively killed CMeC-2 than CMeC-1. We observed PP2A activation in CMeC-2 treated with OP449 and FTY720. These results demonstrated the potential therapeutic application of SET inhibitors for canine melanoma.  相似文献   

A case of primary choroidal malignant melanoma in a cat          下载免费PDF全文

Aurélie Bourguet  Virginie Piccicuto  Elise Donzel  Marine Carlus  Sabine Chahory 《Veterinary ophthalmology》2015,18(4):345-349

This report describes the clinical presentation, diagnosis, histological lesions, and prognosis of a primary choroidal malignant melanoma in a 15‐year‐old cat. The animal was presented for unilateral blindness. On ocular examination, a raised pigmented mass protruding from the posterior pole into the vitreous body was observed by diffuse transillumination and indirect ophthalmoscopy. Ocular ultrasound and computer tomography (CT) scan confirmed localization of the tumor to the posterior segment. The diagnosis of primary choroidal melanoma was confirmed by histopathology after enucleation. To our knowledge, this is the first reported case of a feline malignant melanoma with a primary choroidal localization without iris involvement.  相似文献