Optimization of Agrobacterium-mediated transformation parameters for Melastomataceae spp. using green fluorescent protein (GFP) as a reporter     

Wilson Thau Lym Yong  Janna Ong Abdullah  Maziah Mahmood 《Scientia Horticulturae》2006

Agrobacterium-mediated transformation for both Melastoma malabathricum and Tibouchina semidecandra were optimized using green fluorescent protein (GFP) as a reporter. The binary vector pCAMBIA1304 harboring the modified green fluorescent protein (mgfp) gene driven by the CaMV 35S promoter was used. Parameters optimized were bacterial strain, bacterial concentration, pre-culture period, co-cultivation period, immersion time, acetosyringone concentration and wounding type. Results obtained were based on the percentage of GFP expression which was observed 3 days post-transformation. Agrobacterium tumefaciens strain LBA4404 and EHA105 at concentration 1 × 10⁷ cfu ml⁻¹ (OD_600nm 0.8) showed the highest virulence on M. malabathricum and T. semidecandra, respectively. Four days of pre-culture and 2 days of co-cultivation were optimum for M. malabathricum transformation, while 3 days of pre-culture and co-cultivation for T. semidecandra. Result also showed that 60 min of immersion and addition of 200 μM acetosyringone gave the highest percentage of positive transformants for both M. malabathricum and T. semidecandra. Mild wounding also significantly increased the efficiency of M. malabathricum transformation.  相似文献   

The effect of strain, growth stage, and cultivating condition of Ganoderma lucidum on 5α-reductase inhibition     

Jie Liu  Rumi Fujita  Michiko Sato  Kuniyoshi Shimizu  Fumiko Konishi  Kiyoshi Noda  Shoichiro Kumamoto  Chie Ueda  Hisatoshi Tajiri  Shuhei Kaneko  Yoshitaro Suimi  Ryuichiro Kondo 《Journal of Wood Science》2005,51(2):189-192

The inhibitory effects of 102 methanol extracts of 40 mycelia, 9 culture fluids, and 53 fruiting bodies of 40 strains of Ganoderma lucidum on 5-reductase were investigated. The methanol extract of the fruiting body of each strain was found to show the strongest 5-reductase inhibitory activity among the extracts tested.  相似文献   

重力模型法在居民出行分布预测中的应用     

丁艺  陈菡 《福建农林大学学报(自然科学版)》2007,36(6):618-621

根据福建省浦城县的实际情况,以公交客流的基础数据和调查得到的OD矩阵为材料,选用重力模型法中的双约束重力模型法对该县居民出行分布进行预测,并运用Visual Foxpro对模型进行求解,预测该县未来公交客流分布的特征,为该县公交规划建立合理的公共客运结构提供依据.  相似文献   

鱼糜制品中基因组DNA提取方法的比较   总被引：2，自引：0，他引：2  

陈赟  汪之和 《湖北农业科学》2007,46(4):520-522

将分别采用普通酚-氯仿抽提法和试剂盒(柱吸附法)提取DNA的两种方法加以比较,以确定最适提取鱼糜制品DNA的方法.将鱼肉和鱼糜制品作为原料,用两种方法提取基因组DNA,利用分光光度计测定其A260与A280的吸光值,计算比率估计核酸的纯度,并利用琼脂糖凝胶电泳观察DNA片段在凝胶中的位置;并用线粒体16S rRNA基因PCR扩增产物鉴定所提取的基因组DNA.  相似文献   

Cytotoxic, cytoprotective and antioxidant effects of isolated phenolic compounds from fresh ginger     

Peng F  Tao Q  Wu X  Dou H  Spencer S  Mang C  Xu L  Sun L  Zhao Y  Li H  Zeng S  Liu G  Hao X 《Fitoterapia》2012,83(3):568-585

Twenty-nine phenolic compounds were isolated from the root bark of fresh (Yunnan) ginger and their structures fully characterized. Selected compounds were divided into structural categories and twelve compounds subjected to in-vitro assays including DPPH radical scavenging, xanthine-oxidase inhibition, monoamine oxidase inhibition, rat-brain homogenate lipid peroxidation, and rat pheochromocytoma PC12 cell and primary liver cell viability to determine their antioxidant and cytoprotective properties. Isolated compounds were also tested against nine human tumor cell lines to characterize anticancer potency. Several diarylheptanoids and epoxidic diarylheptanoids were effective DPPH radical scavengers and moderately effective at inhibiting xanthine oxidase. An enone–dione analog of 6-shogaol (compound 2) was isolated and identified to be most effective at protecting PC12 cells from H₂O₂-induced damage. Almost all tested compounds inhibited lipid peroxidation. Three compounds, 6-shogaol, 10-gingerol and an enone-diarylheptanoid analog of curcumin (compound 6) were identified to be cytotoxic in cell lines tested, with KB and HL60 cells most susceptible to 6-shogaol and the curcumin analog with IC₅₀ < 10 μM. QSAR analysis revealed cytotoxicity was related to compound lipophilicity and chemical reactivity. In conclusion, we observed distinct compounds in fresh ginger to have biological activities relevant in diseases associated with reactive oxygen species.  相似文献   

30%螺螨磺酯?阿维菌素可分散油悬浮剂的配方及生物活性          下载免费PDF全文

徐姗姗  鞠光秀  许良忠 《农学学报》2016,6(9):37-41

测试了不同复配比例的螺螨磺酯、阿维菌素对二斑叶螨的毒力,确定最佳药效的原药比例;通过对乳化剂、增效剂的筛选,获得了30%螺螨磺酯?阿维菌素可分散油悬浮剂(OD)的最优配方;测试了其对苹果二斑叶螨、柑橘红蜘蛛的田间防效。30%螺螨磺酯?阿维菌素OD的配方组成为：螺螨磺酯27%、阿维菌素3%、YUS FS3000 4%、601# 5%、T-20 7%、有机膨润土2%、GY-T0304 0.4%、油酸甲酯补足至100%,其各项性能符合可分散油悬浮剂的要求,田间药效试验表明其对二斑叶螨和柑橘红蜘蛛的防效优于对照药剂30%螺螨酯.阿维菌素OD,并且持效期长,具有良好的开发前景。  相似文献   

600MW汽轮机单顺序阀切换时的异常分析及处理     

江广旭  于保国  孔庆民 《湖南农机》2016,(8)

某厂600MW汽轮机阀门控制方式由单阀切换为顺序阀控制时EH油压波动、轴承温度异常现象,无法切换为顺序阀方式运行。通过分析研究,修改阀门开启顺序、修改阀门特性曲线,机组已安全稳定的处于顺序阀控制方式下,取得了良好的经济效益。  相似文献   

气刺微割技术在RRIM600胶树再生皮上的试验初报     

钱云  李芹  李保佺  黄强  赵东兴 《热带作物学报》2015,35(1):14-16

气刺微割技术在RRIM600胶树再生皮上的初步试验结果表明,在再生皮上采用每周期5刀的刺激也有一定的增产效果,株净增产率7.2%,与对照相比产量增幅不大,但在提高割胶速度、降低耗皮量等方面均表现出气刺微割的技术优势。分析认为若提高气刺频率,在RRIM600再生皮上有可能取得更好的增产效果。  相似文献   

氟噻唑吡乙酮10%可分散油悬浮剂高效液相色谱分析     

林绍霞  姜宜飞  吴进龙  王胜翔  宋俊华 《农药科学与管理》2014,35(11):36-38

本文采用高效液相色谱法,以乙腈+磷酸溶液为流动相,使用以ZORBAX SB-C18、5μm为填料的不锈钢柱和二极管阵列检测器,在260nm波长下对氟噻唑吡乙酮10%可分散油悬浮剂进行分离和定量分析。结果表明,该分析方法的线性相关系数为0.999 1,标准偏差为0.15,变异系数为1.51%,平均回收率为100.18%。  相似文献   

真空预处理对圣女果渗透脱水的影响   总被引：1，自引：0，他引：1  

罗慧馨  辛明  罗玲琼  庾林静 《广西农业科学》2013,(11):1894-1898

[目的]以圣女果为原料,探讨真空预处理对圣女果渗透脱水的影响,为今后其工业化应用提供理论参考和技术支持.[方法]通过对预处理的圣女果进行真空渗透脱水,研究真空时间、真空度对渗透圣女果的含水量、固形物含量、糖含量、麦芽糊精渗入量的影响.[结果]真空渗透时间20 min、真空度0.06 MPa的真空渗透预处理渗透速度最快,麦芽糊精的渗入量最大.[结论]确定的圣女果真空渗透脱水最佳方案渗透速度快、脱水效果良好,选用该方法进行圣女果的预脱水是可行的.  相似文献