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将兔２细胞期或８～１６细胞期胚胎培养２４ｈ后的胚胎培养液分别注入去脾兔或去脾小鼠体内。注射后１２０ｍｉｎ内，均出现外周血中血小板数的明显减少。此现象是由于胚胎释放的某种活性物质引起的，该活性物质可能是血小板活化因子，经冷冻保存不丧失生物活性，且不存在种属差异 相似文献
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目的：探讨肌醇脂质和cAMP两个细胞内第二信使系统在血小板活化因子(PAF)诱导血小板聚集过程中所起到的作用和相互关系。方法：采用PKC激动剂PMA和Ca^2 通道A23187,以及PKA激动剂Sp-cAMPS和抑制剂Rp-cAMPS干预的方法,分析观察这两个信使系统在PAF诱导血小板聚集过程中的作用;采用^3H—Inositol和^14C-Adenine双标记液体闪烁技术测定PAF诱导血小板可逆聚集过程中肌醇-1,4,5-三磷酸酯(IP3)和cAMP的水平变化的方法,分析研究这两个信使系统在PAF诱导血小板聚集过程中的相互关系。结果：(1)PMA和A23187能分别增强PAF的血小板聚集效应,而且两者具有协同作用;(2)Rp-cAMP和Sp-cAMPS两者本身都不能引起血小板聚集,但能分别增强和抑制PAF的聚集效应;(3)IP3和cAMP的水平变化分别与血小板的聚集和解聚过程一致。结论：(1)肌醇脂质信使系统是细胞内转导PAF诱导血小板聚集的主要胞内信使系统。(2)降低血小板内cAMP浓度不能诱导聚集,但能增强肌醇脂质信使系统的聚集效应;升高cAMP水平能拮抗肌醇脂质信使系统的作用,这可能是使可逆相聚集的血小板解聚的一个重要机制。 相似文献
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在人工采集的长白猪精液中分别加入0,5,10,25,50,100ng mL的血小板活化因子(PAF),于37℃恒温水浴锅中温育5,15,25,35,45,60min。显微镜下检测精子活率,同时用浊度分析法检测快速运动相精子的平均速度(VRM)和含量(FRM)。结果表明:PAF质量浓度为10ng mL的试验组与对照组相比,温育15～60min,精子活率都显著提高(P<0 05);PAF质量浓度为5ng mL的试验组与对照组相比,温育25～45min,精子活率都显著提高(P<0 05);PAF质量浓度为10,25,50,100ng mL的试验组与对照组相比,有显著提高快速运动相精子运动速度的作用(P<0 05);在试验浓度范围内,试验组与对照组相比,精子含量均无显著差异(P>0 05)。认为一定浓度的PAF可提高长白猪精子的活率和快速运动相精子的运动能力。 相似文献
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海风藤酮对兔胚胎发育及PAF效应的影响 总被引：2，自引：0，他引：2
海风藤团为血小板激活因子PAF的特异性受体桔抗剂。用含20μg·mL-1海风藤酮的培养液培养兔2细胞胚胎144h,发育到等于或小于16细胞的胚胎比例为66．9％,对照组为13．5％（P＜0．025）。发育到襄胚和孵化囊胚的比例,对照组为39．9％,而试验组发育到囊胚的比例为2．7％（P＜0．001）,没有胚胎发育到孵化囊胚阶段。试验表明海风藤团对胚胎的体外发育有阻滞作用。试验还表明,兔胚胎可以释放PAF,海风藤国能明显抑制免胚胎培养液所引起的去牌鼠血小板数量下降的PAF效应。 相似文献
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Bastos da Silva M. Dessy C. Coghe J. David J.-L. Lekeux P. 《Veterinary research communications》1998,22(4):273-291
The ultrastructure of bovine platelets was examined by transmission electron microscopy without any pretreatment (control), and after WEB 2086 (a triazolodiazepine) or ketoprofen (NSAID) pretreatment, followed by PAF infusion. The blood platelet count was also investigated. The group of calves that received WEB 2086 pretreatment before platelet-activating factor (PAF) infusion did not show a decreased number of platelets. However, in the other group, with ketoprofen pretreatment before PAF infusion, there was a rapid decrease from 1 to 3 min, while from 5 min the number of platelets recovered to the normal value. Electron microscopy revealed that pretreatment with WEB 2086 followed by PAF infusion did not alter the morphological ultrastructure of bovine platelets, except that the microtubules were briefly modified from 1 until 3 min after PAF challenge. After ketoprofen pretreatment, bovine platelets kept their regular shape, the number of dense bodies was not significantly altered, the number of mitochondria was maintained from 5 min after PAF infusion, giant platelets were not observed and the Golgi apparatus was rarely visible. Thus pretreatment with WEB 2086 and ketoprofen before PAF infusion had a protective activity on the ultrastructure of bovine platelets and, in cattle, pretreatment with WEB 2086 and ketoprofen before PAF challenge prevented the thrombocytopenia induced by PAF. 相似文献
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兔2细胞期或8～16细胞期胚胎培养液立即或经冷冻、鲜冻后注入去脾小鼠体内,能异源性引起去脾小鼠血小板数量显著减少(P<0.050)。血小板激活因子受体竞争性拮抗剂海风藤酮能抑制这种作用。试验说明兔胚胎释放血小板激活因子,并引起去脾小鼠血小板的聚集。 相似文献
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Alexandros Tsoupras Chloe Brummell Ciara Kealy Karolis Vitkaitis Shane Redfern Ioannis Zabetakis 《Marine drugs》2022,20(3)
The beneficial effects of fish-derived lipid bioactives have come to prominence over the last few decades, especially for their utilization in fish oils, supplements, and nutraceuticals. Omega-3 (n-3) polyunsaturated fatty acids (PUFA), lipid vitamins, carotenoids, and polar lipid bioactives from fish have shown to possess a vast range of beneficial effects against a multitude of chronic disorders and especially against inflammation-and cardiovascular disorders (CVD). The observed cardio-protective effects and health benefits are believed to be attributed to the synergy of these fish-derived lipid bioactives. Within the present article the recent findings in the literature on the lipid content of the mainly consumed fish species, their bio-functionality, and cardio-protective benefits is thoroughly reviewed. Moreover, the recovery and valorization of such lipid bioactives from fish by-products and fishing by-catch, in order to reduce waste, while developing useful products containing cardio-protective lipids from the leftover materials of fisheries and aquaculture industries, are also of industrial and environmental interest. Emphasis is also given to the effects of heat treatments during fish processing on the structures and bio-functionality of these marine lipid bioactives, based on the paradigm of different cooking methodologies and thermal processing, while the compounds produced during such treatment(s) with detrimental changes in the fish lipid profile, which can reduce its cardio-protective efficacy, are also reviewed. Novel green extraction technologies and low temperature processing and cooking of fish and fishery by-products are needed to reduce these undesirable effects in a sustainable and environmentally friendly way. 相似文献
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在Ham’s F-10培养液中,添加了不同剂量的己酮可可碱(PF)或血小板激活因子(PAF),与大熊猫冷冻保存后解冻的精子在37℃水浴中孵育,通过测量孵育不同时间精子的活力、存活时间、顶体反应率、质膜完整率和异种穿卵率,来探讨PF、PAF对大熊猫冷冻精子体外受精能力的影响,结果显示:己酮可可碱、血小板激活因子均能影响大熊猫冷冻精子体外受精能力。其中:己酮可可碱以1 mg/mL处理剂量对提高大熊猫冷冻精子体外受精能力效果较好,总存活时间达(15.33±4.73)h,培养4h时体外异种穿卵率达51.44%,培养6 h时体外异种穿卵率为7.49%,与对照组差异极显著(P<0.01),并优于其它处理组;PAF 50 ng/mL处理剂量下的各质量评估指标优于100 ng/mL处理,但用PAF处理精液的时间超过2 h后即引起精子质量的显著下降。 相似文献
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母畜受孕后的受精卵 ,特别是桑椹胚将产生一类乙酰化的甘油磷脂 ,即血小板活化因子(PAF)。PAF将引起母畜外周血小板下降。通过试验发现母牛、母猪、家兔自发情配种后血小板值逐日下降 ,母牛第7天 ,母猪第6天 ,母兔第3天分别降到最低点。以后又将逐日上升 ,恢复到配种前的水平。根据配种前和配种后最低点的血小板值之差 ,分别取一经验值 ,即可对母畜做出妊娠诊断 相似文献