The expression of IFN-γ and IL-4 on T lymphocytes that infiltrate in nasal polyps     

CHEN Rong  SHI Jian-bo  XU Geng  DI Jing-fang  ZENG Shan  ZENG Yao-ying 《园艺学报》2003,19(12):1623-1626

AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P＜0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps.  相似文献   

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs     

LIU Cui-cui  GUO Xiao-hong  CAO Guo-qing  GAO Peng-fei  PU Zhong-de  NIU Jiao-yan  ZHANG Qi  LI Meng  JIN Yu-shu  LI Bu-gao 《中国畜牧兽医》2015,42(10):2726-2731

The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.  相似文献   

基于VHDL的交通灯控制系统设计     

《科技视界》2017,(20)

依据EDA自顶向下的设计流程进行交通灯控制系统设计,采用VHDL语言编写各功能模块,生成各模块符号图,把各模块符号图以原理图的形式连在一起得到系统顶层设计。并在Quartus II9.0集成开发环境里进行编译、仿真和综合,最后下载到实验箱进行调试,调试结果表明:交通灯的状态切换,倒计时时间显示均可实现。  相似文献   

基于Cortex-M4内核的μCOS-Ⅱ移植     

《科技视界》2016,(9)

针对TI公司新推出的基于Cortex-M4内核的TM4C123G高性能低功耗芯片,详细介绍了嵌入式开源实时操作系统μCOS-Ⅱ在芯片上的移植方法。根据移植的需求,首先介绍了芯片的一些基本功能以及相关的软件开发环境,然后结合芯片的固有特性以及μCOS-Ⅱ移植的需求,使用C语言和汇编语言修改了相关的源文件,并详细阐述了修改的原因。  相似文献   

Effect of tanshinone ⅡA on liver lipid deposition and ferroptosis-related protein expression in ApoE^-/- mice     

WU Yao  SONG Nan  JIA Lian-qun  WANG Jie  CHEN Si  CAO Hui-min 《园艺学报》2020,36(7):1261-1268

AIM To observe the effect of tanshinone ⅡA on liver lipid deposition and ferroptosis-related protein expression in ApoE^-/- mice. METHODS Thirty-two ApoE^-/- mice were randomly divided into model group, high-dose (60 mg/kg) tanshinone ⅡA group, low-dose (30 mg/kg) tanshinone ⅡA group and simvastatin group, and C57BL/6J mice (n=8) were used as normal control group. The mice in normal control group were given the basic feeding, while the others were given high-fat diet. The mice in tanshinone ⅡA groups and simvastatin group were given corresponding drugs. The mice in normal control group and model group were intraperitoneally injected with equal volume of saline. Eight weeks later, the serum levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were tested by automatic biochemistry analyzer. The liver tissues were stained with HE and oil red O. The contents of reactive oxygen species (ROS) and glutathione (GSH) in liver tissues of the mice were measured by commercially available kits. The liver glutathione peroxidase 4 (GPX4) and p53 were detected by immunohistochemical method. The protein and mRNA expression levels of ferroptosis-related factors GPX4, xCT/SLC7A11, p53 and ferritin heavy chain 1 (FTH1) were determined by Wes automatic Western blot quantitative analysis system and RT-qPCR. RESULTS Compared with normal control group, the serum levels of TC, TG and LDL-C in model group were increased significantly (P<0.05 or P<0.01), and HDL-C did not change significantly. The fat vacuoles were clearly visible in liver tissue. The content of ROS in liver tissue was increased significantly,and GSH was decreased significantly (P<0.01). The mRNA and protein expression levels of p53 were increased significantly, and GPX4, xCT/SLC7A11 and FTH1 were decreased significantly (P<0.05 or P<0.01). Compared with model group, tanshinone ⅡA significantly decreased the serum levels of TC, TG and LDL-C (P<0.05 or P<0.01), and HDL-C did not change significantly. High-dose and low-dose tanshinoneⅡA also significantly decreased the degree of steatosis, and the size of lipid droplets. The content of ROS in liver tissues was decreased significantly, and GSH was increased significantly (P<0.01). The mRNA and protein expression levels of GPX4, xCT/SLC7A11 and FTH1 were increased significantly, and p53 were decreased significantly (P<0.05 or P<0.01). CONCLUSION Tanshinone ⅡA reduces liver lipid deposition and lipid peroxidation damage in ApoE^-/- mice, which may be related to the intervention of ferroptosis-related proteins in the liver cells.  相似文献   

多约束条件下某智能飞行器航迹快速规划     

冯礼鸿  蒋胜 《农业装备与车辆工程》2021,(1):116-120

以某智能飞行器为研究对象,研究复杂环境下航迹快速规划.为校正系统结构限制所带来的定位误差,使得智能飞行器能按照预定的航迹完成任务,基于多约束条件最优化理论,采用遗传算法扩展而来的NSGA-Ⅱ(非支配排序遗传算法)算法进行航迹规划.结果表明,基于NSGA-Ⅱ,使用交叉行为和变异行为将初始解进一步筛选优化并最终得到最优解....  相似文献   

茄子离体叶片光系统Ⅱ的冷激胁迫效应及热力学分析     

王梅  高志奎  黄瑞虹  吕桂云  张文丽  杜爽 《园艺学报》2009,36(2):261-266

 以耐冷性较强的短把黑圆茄和耐热性较强的黑帅圆茄为试材,经冷激胁迫后采用植物效率仪PEA,进行快速叶绿素荧光参数测定,并利用最大光化学效率Fv/F m和活性中心RC/CSo的标准状态变性自由能变△GD进行PSⅡ冷稳定性的热力学分析。随着冷激胁迫温度的降低和时间的延长,PSⅡ的Fv/Fm、实际光化学效率ΔF/Fm′、RC/CSo呈"S"型下降趋势;单位反应中心DIo/RC和非光化学猝灭qN呈"S"型上升趋势;综合分析反映出冷激胁迫下PSⅡ反应中心的可逆失活和依赖于叶黄素循环的热耗散的双重机制在保护PSⅡ防止光抑制中起到重要作用。另外,通过两品种间PSⅡ行为差异,引入了两组新的参数作为便于直观比较冷胁迫的评价指标。黑帅圆茄Fv/Fm、ΔF/Fm＇的半衰温度T1/2和t1/2值分别高于短把黑圆茄1.4 ℃和0.8 ℃。茄子叶片Fv/Fm和RC/CSo的△GD随着冷激胁迫温度的降低而减少呈瀑布型的下降趋势。黑帅圆茄的△GD值和变性中点温度Tm都低于短把黑圆茄。它们均反映出黑帅圆茄的耐冷性低于短把黑圆茄。  相似文献   

杂草对光系统Ⅱ抑制剂的抗药性研究进展     

龙迪  王彦辉  曾东强 《分子植物育种》2021,19(4):1383-1392

随着除草剂使用量和使用频率的不断增加,杂草抗药性问题也逐渐成为杂草防除及治理的难点和热点。目前杂草对PS(Photosystem)Ⅱ抑制剂类除草剂产生抗药性主要分为靶标抗性和非靶标抗性。本研究综述了近年来杂草对PSⅡ抑制剂类除草剂抗药性的产生、发展现状及杂草抗PSⅡ抑制剂类除草剂的机理。针对这些问题,提出除草剂使用过程中需要注意的事项,为延缓杂草对该类除草剂产生抗药性提供一定参考。  相似文献   

水分胁迫对不同甘蔗品种叶绿素a荧光动力学的影响   总被引：13，自引：0，他引：13  

罗俊  张木清  吕建林  林彦铨 《福建农林大学学报(自然科学版)》2000,29(1)

利用 OS5- FL调制式叶绿素荧光分析仪研究水分胁迫对不同甘蔗品种叶片叶绿素 a荧光动力学的影响 .结果表明 :水分胁迫下蔗叶可变荧光产量 (Fv值 )下降 ,T1/ 2 减少 ,蔗叶光系统 (PS )原初光能转换效率(Fv/ Fm)和 PS 潜在活性 (Fv/ Fo)降低 ;蔗叶可变荧光衰减能力 (Δ Fv)下降 ,光合作用潜在活力降低 ,影响光合电子传递和 CO2 同化的正常进行 ,表现为可变荧光淬灭速率 (Δ Fv/ Fo)减慢 ,荧光下降比值 (Δ Fv/ Ft值 )变小 ,光合量子产额 (Yield)减少 .同时还讨论了水分胁迫对蔗叶光合作用影响的机制  相似文献   

Tanshinone ⅡA induces apoptosis via JNK signaling pathway in human osteosarcoma HOS cells     

LU Jian-hong  HUANG Xiao-wen  JIN Yi-jun  YANG Yan-nan  WU Yi-zheng 《园艺学报》2019,35(1):28-33

AIM: To explore the effect of tanshinone ⅡA on human osteosarcoma HOS cells and the underlying mechanism.METHODS: The cell viability and the appropriate dose of tanshinone ⅡA were determined by CCK-8 assay. Colony formation assay and Transwell assay were used to investigate the proliferation and migration abilities of the HOS cells treated with tanshinone ⅡA. The apoptosis of the HOS cells was monitored by Hoechst 33258 staining, transmission electron microscopy and flow cytometry. The protein levels of apoptosis-related molecules and JNK signaling-associated proteins were determined by Western blot. Meanwhile, a JNK inhibitor was added for confirming the relationship between the pathway and apoptosis mentioned above.RESULTS: Tanshinone ⅡA inhibited both HOS cell proliferation and migration in a dose-and time-dependent manner. Exposure of the HOS cells to tanshinone ⅡA resulted in the activation of apoptosis. Tanshinone ⅡA treatment increased the protein levels of cleaved caspase-3, Bax and JNK signaling-associated proteins, and decreased the protein level of Bcl-2, which were reversed by JNK inhibitor SP600125. Moreover, the result of CCK-8 assay revealed that tanshinone ⅡA-induced cell death was alleviated by JNK inhibitor.CONCLUSION: Tanshinone ⅡA induces cell growth inhibition and the activation of apoptosis via JNK signaling pathway in human osteosarcoma HOS cells.  相似文献