饲粮不同蛋白质、能量水平对大白鼠生长发育及体成分的影响     

陈文清  许振英 《动物营养学报》1992,(2)

本试验所用动物为53—57日龄雄性大白鼠(sprague——Dawley品系),初始体重为121.73±3.95g,试验期为大白鼠生长发育旺期的21天。试验拟研究不同粗蛋白水平(10％、15％、20％、25％和30％)及两个能量水平(消化能:3.6和3.9kcal/g)的饲粮对大白鼠生长发育及各种体成分的影响。结果表明,当饲粮蛋白质供应充分时,大白鼠生长随饲粮能量浓度的提高而改善;而当饲粮蛋白质供应感觉不足时,提高能量浓度对生长无益。机体各项成分指杯中,以蛋白质、脂肪、水分沉积受饲粮蛋白质,能量浓度影响显著;而蛋白质、脂肪沉积在很大程度上受饲粮能朊比的影响,饲粮中添加脂肪可使大白鼠机体肥胖。  相似文献   

家蚕丝素蛋白对大鼠胆固醇代射的影响   总被引：1，自引：0，他引：1  

桂仲争  庄大桓 《蚕业科学》2002,28(4):301-303

研究了经酸解后制得的家蚕丝素蛋白氨基酸组成以及对高血脂实验鼠血清和肝脏内胆固醇及转氨酶的活性变化的影响。结果表明：所得的家蚕丝素蛋白粉氨基酸组成丰富，其中甘氨酸、丙氨酸、丝氨酸和酪氨酸含量占全部氨基酸总量的95%；家蚕丝素蛋白能显著地降低血清胆固醇含量和谷草转氨酶及谷丙转氨酶活性，提高动物肝组织中的胆固醇浓度和谷丙转氨酶的活性。  相似文献   

Action of deltamethrin on N-type (Ca_v2.2) voltage-sensitive calcium channels in rat brain     

Steven B. Symington 《Pesticide biochemistry and physiology》2005,82(1):1-15

Isolated presynaptic nerve terminals prepared from whole rat brain were used to evaluate the action of deltamethrin on voltage-sensitive calcium channels by measuring calcium influx and endogenous glutamate release. Deltamethrin-enhanced K⁺-stimulated calcium influx and subsequent Ca²⁺-dependent glutamate release. The effect of deltamethrin was concentration-dependent, stereospecific, blocked by ω-conotoxin MVIIC but unaltered in the presence of tetrodotoxin. These results suggest that N-type voltage-sensitive calcium channels are a site of action at the presynaptic nerve terminal. Electrophysiological studies were carried out using rat brain Ca_v2.2 and β₃ subunits coexpressed in Xenopus oocytes to validate such action. Deltamethrin reduced barium peak current in a concentraion-dependent and stereospecific manner, increased the rate of activation, and prolonged the inactivation rate of this channel. These experiments support the conclusion that N-type voltage-sensitive calcium channel operation is altered by deltamethrin.  相似文献   

LIF与心肌条件液在小鼠ES细胞分离中的差异比较     

刘俊平  曹鸿国  安志兴  张涌 《中国农学通报》2005,21(3):30-30

通过比较LIF和大鼠心肌条件液在小鼠ES细胞分离培养过程中的差异, 从而选择较为合适的培养液用于小鼠ES细胞的分离培养与深入研究。取怀孕3.5 d小鼠囊胚, 培养于小鼠胎儿成纤维细胞饲养层上, 然后根据ES细胞培养液的不同分成2组,一组添加LIF的ES细胞培养液,另一组添加由大鼠心肌条件液组成的ES细胞培养液。结果显示,小鼠ICM的孵出率在心肌条件液中为76.30 %, LIF条件液中为59.35 %,两者差异极显著(p＜0.01)))))) ; 在LIF条件液中比心肌条件液中能较早地分离出ICM,时间差分别为11、11、12、10和12 h,平均为11.2 h; 对传代的ES细胞集落,培养48 h时周边出现分化现象的ES细胞集落所占的比例在心肌条件液中为51.55%, LIF条件液中为31.69 %,两者差异显著(p＜0.05); 第五代小鼠ES细胞核型正常率在心肌细胞条件液中为78.6 %, 稍高于LIF条件液中的76 %,差异不显著。  相似文献   

利用植物控制鼠害的应用研究现状及展望   总被引：8，自引：0，他引：8  

韩崇选  张宏利  杨学军  王明春  杨清娥  贺春玲 《西北农业学报》2004,13(3):89-92,96

鼠类主要以植物为食,而植物对鼠类的取食也具有一定的抵抗作用,这种作用主要表现在驱避、毒杀和使鼠类产生不育作用。植物对鼠类的驱避作用可使其避免害鼠的危害,毒杀和不育作用可使鼠类种群数量减少,从而减轻对植物自身的危害。本文主要对植物的这3种作用进行介绍,并分析了植物次生代谢物在鼠害防治中的发展趋势和应用前景。  相似文献   

生长抑素对大鼠乳腺斯钙素基因表达的影响     

汪新建  李惠  李和平  刘凤娟  查光明  郭豫杰  王月影 《江西农业学报》2011,(10):14-16

将泌乳期大鼠分为对照组、CS组和SS组,利用RT-PCR法对大鼠乳腺组织斯钙素基因进行检测,分析生长抑素对大鼠乳腺斯钙素基因表达的影响。结果表明:乳腺中有STC1表达,无STC2表达。生长抑素对泌乳期大鼠乳腺斯钙素基因表达起下调作用,半胱胺作用相反。以上结果提示生长抑素和半胱胺可能参与乳腺钙代谢的调节,且通过STC1发挥作用。  相似文献   

May antioxidants status depletion by Tetradifon induce secondary genotoxicity in female Wistar rats via oxidative stress?     

Riadh Badraoui  Nouha Bouayed Abdelmoula  Moncef Fki 《Pesticide biochemistry and physiology》2007,88(2):149-155

Tetradifon is a potent organochlorine acaricide with an estrogen-like structure. The wide spread use of this chemical is likely to pollute the environment. Only few studies have been reported for the evaluation of its short- and long-term toxic effects including genotoxicity and carcinogenicity and there have been conflicting results in in vitro and in vivo test systems. In this work, we have evaluated Tetradifon for its ability to induce genotoxic damages in female Wistar rats. A single cumulative dose of 2430 mg/kg BW was administrated orally for 12 female rats of 190 g BW during 12 weeks. Twelve additional rats, no treated, have served as control. Animals were sacrificed after 6 and 12 weeks of treatment. Genetic toxicity studies were conducted in rats bone marrow, by chromosomal aberrations (CAs) and sister-chromatid exchanges (SCEs) assays. The oxidative stress status of treated animals has been also evaluated by assessment of lipid peroxidation by measuring thiobarbituric acid reactive substances (TBARS). Some serum parameters: vitamins (A and E), triglyceride (TG) and total antioxidants status (TAS) were determined. Our results showed a significant increase in tissue TBARS concentrations in the two treated groups suggesting that Tetradifon induce an oxidative stress. Elsewhere, rats treated with Tetradifon exhibited a statistical decrease in serum level of vitamin E and a significant depletion of serum total antioxidant status. Whereas, in comparison to control rats, treated animal cells did not show a significant increase in either the frequency of SCEs or CAs. These results indicate that Tetradifon did not present direct genotoxic effect in female Wistar rats. But we suggest that its inducting of an oxidative stress may lead to indirect mutagenecity that should be evaluated by other series of in vivo genotoxicity assays as micronucleus test or comet assay.  相似文献   

Evaluation of propolis effects on some biochemical parameters in rats treated with sodium fluoride     

Gokhan Eraslan  Murat Kanbur 《Pesticide biochemistry and physiology》2007,88(3):273-283

The present study in which 42 female rats, each weighing 200−250 g, were used covered a period of 21 days. The animals were divided into six groups. The first group served as the control group, whereas Group 2 was administered propolis at a dose of 200 mg/kg/bw in drinking water for 21 days. Group 3 was first provided with normal drinking water for a period of 14 days, and was subsequently administered propolis at a dose of 200 mg/kg/bw in drinking water for 7 days. Group 4 was first given normal drinking water for 14 days, and was secondly administered 100 ppm fluoride as a sodium fluoride in drinking water for 7 days. Group 5 was first administered propolis alone at a dose of 200 mg/kg/bw in drinking water for 14 days, and was secondly administered 100 ppm fluoride in association with 200 mg/kg/bw propolis for 7 days. Finally, Group 6 was first provided with normal drinking water for 14 days, and was secondly administered 100 ppm fluoride in association 200 mg/kg/bw propolis for a period of 7 days. At the end of the 21st day, blood samples were collected from the heart of each animal into both heparinised tubes and tubes without anticoagulants. Glucose, triglyceride, cholesterol, total protein, and uric acid levels, and aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) activities in the serum, as well as malondialdehyde (MDA) levels, glutathione peroxidase (GSH-Px) in the plasma, erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities were measured. When compared to the control group, statistical differences were determined to exist with respect to oxidative stress parameters which involved increase in MDA levels in Groups 4−6, decrease in SOD activity in Groups 4 and 6, increase in CAT activity in Groups 5 and 6, and decrease in GSH-Px activity in Groups 4 and 6. Furthermore, in comparison to the control group, significant differences were observed with respect to certain serum biochemical parameters, including decrease in glucose levels in Groups 5 and 6, decrease in triglyceride levels in Groups 2 and 4, decrease in cholesterol levels in Groups 2 and 5, decrease in the total protein level of Groups 4−6, decrease in the ALT activity of Groups 5 and 6, increase in the AST activity of Group 4, decrease in the ALP activity of Groups 2−6 and increase in the uric acid level of Group 2. In the groups that were administered propolis in association with fluoride, improvement was observed in some oxidative stress parameters and certain other biochemical parameters. Changes determined in the oxidative stress parameters (especially MDA and SOD) were indicative of the anti-radical activity of propolis on the free radicals generated by sodium fluoride. However, the values not drawing completely close to those of the control group can be explained with propolis not being able to completely eliminate the free radicals and the other adverse effects generated by fluoride.  相似文献   

毕节地区新造林地鼠兔危害状况调查及防控对策     

刘正忠  唐玉萍  赵英祥 《贵州林业科技》2007,35(3):43-47

对啮齿类动物鼠兔害的防治.危害调查、标本制作、种类鉴定、生活习性观察,探索有效控制措施.本文着重从森林环境资源的核算评估鼠兔害对新造林地经济损失.  相似文献   

神经节苷脂对脑瘫大鼠大脑皮质区Nestin、NGF表达的影响     

金玲  何小进  尹玲桃  江兴林   《勤云标准版测试》2014,34(8):13-16

目的 观察神经节苷脂(GM1)对脑瘫大鼠大脑皮质区神经巢蛋白(Nestin)、神经生长因子(NGF)表达的影响,探讨GM1促进脑瘫神经修复的可能机制.方法 选择出生10周雄性SD大鼠200只,随机分为3组:假手术组60只,脑瘫模型140只,再分为模型组70只,腹腔注射生理盐水;GM1组70只,腹腔注射GM1.分别在术后0～24 h的7个时间点利用酶联免疫法检测大鼠大脑皮质区Nestin、NGF表达情况,并在实验前和后1～4 d观察大鼠的体质量变化情况.结果 (1)大鼠大脑皮质区Nestin表达GM1组0～24 h、模型组0～12 h明显高于假手术组(P＜0.01);GM1组16～24 h明显高于模型组(P＜0.01).(2)GM1组大鼠大脑皮质区NGF表达0～24 h明显高于模型组(P＜0.05)和假手术组(P＜0.01).模型组NGF表达仅0、4、8h高于假手术组(P＜0.05).(3)GM1组术后第1、2天体质量缓慢增加,至第3、4天明显高于模型组(P＜0.05),接近假手术组(P＞0.05),模型组体质量增加不明显.结论 预防性使用GM1是通过增强大脑皮质的Nestin和NGF表达并延长表达时间来发挥其对实验性脑瘫大鼠的神经保护作用.  相似文献