A reactive pattern of ischemia-induced nestin protein in the rat brain     

LEI Wan-long  YUAN Qun-fang  DONG Zhi-hong  YAO Zhi-bin 《园艺学报》2002,18(6):639-642

AIM:To explore the expressive profile of nestin protein in the focal ischemic brain and to study the recovery mechanism of brain focal infarct.METHODS:Cellular morphology,time-course and distribution pattern of nestin positive response were immunohistochemically examined in different brain regions of 36 adult male SD rats. RESULTS:Nestin positive response of different brain regions in sham operated rats was present in small- and micro-vasculartures and the third ventricle bottom and ependyma. A large number of nestin positive cells were detected in ischemic brain, and were more remarkable in the cortical areas of parietal lobe and preoptic area as well as ischemic caudoputamen. Stellate nestin positive cells were located in the deep layer of ischemic cortex, but fibrillary cells were located in the shallow layer. Nestin positive cells in the ischemic caudoputamen showed the same changes of morphology as those cells in the deep layer of ischemic cortex. Morphological and number alterations of nestin positive cells were the most remarkable at 1 weeks post-ischemia, which showed more hypertrophy and proliferation in morphology, and a marked increase in number was present in the ischemic cerebral cortex and the ischemic caudoputamen. These alterations of nestin positive cells persisted up to 6 weeks post-ischemia, and then, the nestin positive response in the ischemic brain decreased gradually.CONCLUSION:Focal cerebral ischemia induces nestin re-expression on reactive astrocytes, which may be very important to the self-recovery of cerebral infarct.  相似文献   

酶联免疫吸附法测定畜禽组织中恩诺沙星的残留量     

杨华生 《安徽农业科学》2015,(24):114-115

利用抗原抗体反应的高度特异性和酶的高效催化作用相结合,测定畜禽组织中恩诺沙星的残留量。结果表明,该检测方法简便、快速、灵敏。  相似文献   

Level and Timing of Nitrogen Fertilizer Application to Early and Semi-early Potatoes (Solanum tuberosum L.) Grown with Irrigation on Light Soils in Norway     

Hugh Riley 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):122-134

Trials were performed with early and semi-early potatoes to test the effects of nitrogen (N) fertilizer level (0-160 kg N ha^-1) and timing (all at planting versus half then and half either soon after emergence or 3 weeks later). All seven trials with earlies were irrigated as required, whilst different irrigation regimes (moderate versus intensive) were compared in two trials with semi-earlies. No benefit was derived from splitting the N application. Haulm growth and N uptake increased in all cases almost linearly up to the highest N level, but tuber yield did not respond in the same way. The optimum N level was 80 kg N ha^-1 for a yield of 15 Mg ha^-1, rising to 120 kg N ha^-1 for a yield of 40 Mg ha^-1. Tuber quality was lowered by the use of excess N fertilizer, particularly in the case of earlies. The quantity of mineralised N present in the soil after harvest rose sharply with above optimum fertilizer use, and the amount of N present in crop residues also increased. The likely leaching after early potatoes was estimated to be up to 80 kg N ha^-1. The proportion of fertilizer N which was not accounted for in either tuber yield, crop residues or mineral N in soil was 26% in earlies and 38% in semi-earlies.  相似文献   

高压静电回收氯化锌废气设备的研究     

赵道彬  谢仁智  李英华  吴波 《林产化学与工业》1984,(1)

本文用生产规模的单管高压静电设备。对回收氯化锌法活性炭生产过程中产生的氯化锌废气进行了研究;对影响锌回收率的各因子进行了讨论。研究结果表明,工业生产中产生的氯化锌废气可以用高压静电法回收,当二次交流电压为3·5万伏,管内废气流速保持一定值,锌回收率可达98％以上,回收液的浓度29°Be′/36℃。设备性能稳定,运行可靠,不需要冷却水,工艺流程短,运行费用低,经济效果显著,是目前国内同行业中光进的回收设备。  相似文献   

Mortality and recovery of runt white sturgeon (Acipenser transmontanus) in a commercial farm in California, USA     

Georgiadis MP  Hedrick RP  Johnson WO  Gardner IA 《Preventive veterinary medicine》2000,43(4):269-281

We investigated the effect of raising runt white sturgeon (Acipenser transmontanus) separately from dominant fish during the initial stages of grow-out in a commercial farm. Runt fish are poor-growers, have underdeveloped muscle mass, swim slowly and are more-frequently found at the top of the water column. The objective of the study was to describe the mortality and recovery rates (and their determinants) of white-sturgeon runts after separating them from dominant fish. Runt white sturgeon were stocked into twelve 2 m × 2 m rectangular tanks and graded periodically during a follow-up of 46–102 days. Overall mortality rates ranged from 0.3 to 7 dead fish per 1000 sturgeon-days at risk and overall recovery rates from 3.9 to 13.5 recovered fish per 1000 sturgeon-days at risk. Period-specific mortality and recovery rates increased over time. The period-specific mortality rates for all three periods were significantly higher for tanks of runts originating from grow-out tanks with high mortality (p-values: first period = 0.06; second period = 0.09; third period = 0.03), but were similar for tanks of runts of high- and low-mean initial weight. The period-specific recovery rates were significantly higher in runts originating from high-mortality grow-out tanks only for the third period (p = 0.05) but not the first and second periods (p-values = 0.33 and 0.25, respectively). Recovery rates were significantly higher in the higher-mean-weight runts tanks for the first and third period but not for the second (p-values: first period = 0.02; second period = 0.65; third period = 0.06). We concluded that the proportion of runts that recover during a 46–89 day period is substantial (16–58%); therefore, it might be worthwhile growing such fish separately in a fish farm for about three months. Financial analysis showed that this practice was profitable, if the value of white sturgeon fish for the farm exceeded $2.05 per kg.  相似文献   

Hyperthermia and Delayed-Onset Myopathy after Recovery from Anesthesia in a Horse   总被引：1，自引：0，他引：1  

Nicola Grint BVSc  DVA  DiplECVA  MRCVS  Dylan Gorvy BSc  BVSc  PhD  CertES  MRCVS  Alexandra Dugdale MA  VetMB  DVA  DiplECVA  MRCVS 《Journal of Equine Veterinary Science》2007,27(5):221-227

A 13-year old Thoroughbred cross gelding (528 kg) underwent lameness investigation. Because of his temperament, general anesthesia was required to facilitate ultrasound of the left fore fetlock and intra-articular medication of three joints. Anesthesia was induced with ketamine/diazepam after acepromazine/detomidine premedication. Anesthesia was maintained for 40 minutes with a guaifenesin/detomidine/ketamine intravenous infusion. Recovery from anesthesia was initially uneventful, although of a moderate duration (70 minutes). Once standing, the horse proceeded to box walk in an agitated state and became recumbent on two occasions. The horse was manually restrained, at which time its rectal temperature was 41.8°C. Cooling measures were employed (fans, ice-water enemas, wet rugs, intravenous fluid therapy (IVFT), and topical application of surgical spirit) until rectal temperature reached 38.7°C. IVFT was continued for a further 16 hours. Four days after recovery from anesthesia, bilateral triceps, deltoideus, trapezius, and rhomboideus muscle swelling was observed. Blood creatinine kinase was elevated (24,898 IU/L). Treatment for postanesthetic myopathy was initiated (hot packing of the muscle groups, topical dimethylsulfoxide [DMSO] cream application, and oral phenylbutazone). Myoglobinuria was not observed at any time. Muscle swelling decreased over the following 3 days. The horse was discharged on day 11 and has since returned to work.  相似文献   

一种用C18柱纯化低浓度微量寡核苷酸的新方法     

赵松子  沈向群  漆小泉 《江西农业学报》2009,21(7):9-11

通过比较4种不同的回收方法和C18硅胶4种不同的用量对回收的影响,提出了回收低浓度微量寡核苷酸的最佳方案:分别用1 mL乙腈、2 mL灭菌双蒸水和1 mL 10 mmol/L乙酸铵平衡装填10 mg硅胶的C18 Sep-Pak反相层析柱;再将寡核苷酸溶液通过C18柱;然后用1 mL 25 mmol/L碳酸氢铵洗脱C18柱,并以3000 r/m in离心2 m in;最后用1 mL 30%乙腈洗脱C18柱,将收集的溶液离心冻干后即得到纯化的寡核苷酸。该方法的回收率可达53.7%±3.0%。  相似文献   

小叶白掌变异苗的恢复     

罗为伟  胡庆  段学涛 《江西林业科技》2003,(5):13-15

小叶白掌变异苗的恢复应在25℃一28℃条件下。对4类变异苗，以MS IAA0．1mg／L培养基的效果为好；对5类变异苗则以MS IBA0.3mg/L培养基较好。  相似文献   

人工恢复与重建森林生态系统健康及其维护初探   总被引：5，自引：0，他引：5  

刘苑秋  刘光顺 《江西林业科技》2001,(5):15-17,32

人工恢复与重建森林生态系统，随着时间的推移，由于系统内生物个体与个体、个体与环境之间的关系变化而发生动态的变化，如果不能对系统的健康状况正确认识和维护，恢复的系统则可能再次退化，使荒山绿化、植被恢复不能达到预期目标。探讨了人工恢复与重建森林生态系统的健康评价方法及其维护对策。  相似文献   

洱海东岸面山石灰岩山地森林植被恢复与重建刍议     

杨彪 《四川林勘设计》2004,(2):29-32

分析洱海东岸面山石灰岩山地的生境、植被条件和植被演替的一般规律，提出以封山育林为主，人工造林为辅的措施是本地森林植被恢复与重建的重要途径。  相似文献