全文获取类型
收费全文 | 11596篇 |
免费 | 467篇 |
国内免费 | 1437篇 |
专业分类
林业 | 652篇 |
农学 | 1135篇 |
基础科学 | 590篇 |
1404篇 | |
综合类 | 4926篇 |
农作物 | 692篇 |
水产渔业 | 501篇 |
畜牧兽医 | 2347篇 |
园艺 | 687篇 |
植物保护 | 566篇 |
出版年
2024年 | 38篇 |
2023年 | 128篇 |
2022年 | 254篇 |
2021年 | 331篇 |
2020年 | 390篇 |
2019年 | 522篇 |
2018年 | 431篇 |
2017年 | 506篇 |
2016年 | 622篇 |
2015年 | 498篇 |
2014年 | 573篇 |
2013年 | 650篇 |
2012年 | 711篇 |
2011年 | 713篇 |
2010年 | 653篇 |
2009年 | 662篇 |
2008年 | 600篇 |
2007年 | 568篇 |
2006年 | 519篇 |
2005年 | 362篇 |
2004年 | 309篇 |
2003年 | 262篇 |
2002年 | 225篇 |
2001年 | 202篇 |
2000年 | 203篇 |
1999年 | 200篇 |
1998年 | 177篇 |
1997年 | 257篇 |
1996年 | 242篇 |
1995年 | 215篇 |
1994年 | 239篇 |
1993年 | 231篇 |
1992年 | 274篇 |
1991年 | 270篇 |
1990年 | 227篇 |
1989年 | 91篇 |
1988年 | 43篇 |
1987年 | 26篇 |
1986年 | 17篇 |
1985年 | 8篇 |
1984年 | 9篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1956年 | 5篇 |
1955年 | 5篇 |
1944年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
1.
2.
Shelley J. Newman Stephen A. Smith Kurt Zimmerman 《Journal of veterinary diagnostic investigation》2021,33(3):566
A large, firm, multi-cystic mammary gland mass grew slowly over 4 y in a 12-y-old, female Finn–Shetland cross sheep. A diagnosis of epithelial malignancy was suspected following fine-needle aspiration cytology at 30 mo after initial observation. The sheep was euthanized when the flock was downsized 18 mo later. A field postmortem examination revealed a large mammary mass, but an absence of metastases to internal organs. Imprint cytology of the mammary tissue supported a benign proliferative process. Histologically, mammary tissue was obliterated by cystic, tubular, and papillary adenomatous arrangements of mammary epithelium, with an anaplastic component, consistent with mammary carcinoma arising in an adenoma. IHC showed strong nuclear positivity to the antibody against progesterone receptor and minimal positivity to the antibody against estrogen receptor alpha expression. Intrinsic subtyping for basal or luminal epithelial origin was attempted through adaptation of companion animal IHC classification panels; high- and low-molecular-weight cytokeratins (CK5, CK8, CK18) failed to stain, but p63 expression for basal epithelium was positive. 相似文献
3.
Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens
下载免费PDF全文
![点击此处可从《农业科学学报》网站下载免费的PDF全文](/ch/ext_images/free.gif)
ZENG Xian-ying CHEN Xiao-han MA Shu-jie WU Jiao-jiao BAO Hong-mei PAN Shu-xin LIU Yan-jing DENG Guo-hua SHI Jian-zhong CHEN Pu-cheng JIANG Yong-ping LI Yan-bing HU Jing-lei LU Tong MAO Sheng-gang GUO Xing-fu LIU Jing-li TIAN Guo-bin CHEN Hua-lan 《农业科学学报》2020,19(9):2294-2300
We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control. 相似文献
4.
AIM: To investigate the role of zerumbone (ZER) in 1-methyl-4-phenylpyridinium (MPP+)-induced cytotoxicity of human neuroblastoma SH-SY5Y cells. METHODS: Human neuroblastoma SH-SY5Y cells were cultured in vitro and the protective effect of ZER against MPP+-induced cytotoxicity was measured by CCK-8 assay. Flow cytometry was used to determine the apoptosis and reactive oxygen species (ROS). The expression of Parkinson disease protein 7 (PARK7) was knocked-down by using PARK7-specific short hairpin RNA (shRNA). The protein levels of PARK7, nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot. RESULTS: MMP+ remarkably reduced the cell viability in a dose-dependent and time-dependent manner. The SH-SY5Y cell injury model was established by treatment with MPP+ at 600 μmol/L for 24 h. ZER up-regulated the protein levels of PARK7 and Nrf2 (P<0.05), alleviated apoptosis (P<0.05), and reduced ROS production (P<0.05) in the SH-SY5Y cell injury model. Meanwhile, N-acetyl-L-cysteine (NAC) had the similar functions. Moreover, significant reductions in the protein levels of Nrf2 and HO-1 (P<0.05), and obvious increases in apoptosis (P<0.05) and ROS level (P<0.05) were demonstrated in PARK7-knockdown cells. CONCLUSION: ZER protects SH-SY5Y cells against MPP+-induced cytotoxi-city, which may be related to activation of PARK7/Nrf2/HO-1 pathway, and subsequent attenuation of oxidative stress and apoptosis. 相似文献
5.
CRISPR/cas是一种获得性免疫防御系统,广泛存在于细菌和古细菌中。Strep tococcus pyogenes cas9(Spcas9)作为目前研究最多、最清楚的效应蛋白,因其酶活性高和靶点广而被应用于生命科学各个领域,但Spcas9分子量大、脱靶率高等缺点在一定程度上限制了其应用。随着CRISPR系统技术被深入开发,一些新效应蛋白被发现,本文就CRISPR/cas系统分类、原理、新效应蛋白发现及cas9(S.Pyogenes)多种变构体技术开发在及家禽上的应用进行综述,为相关领域的研究提供参考。 相似文献
6.
The aim of this research was to evaluate a passenger seat of a new type bus in terms of eomfort. A fuzzy set model of multistage comfort scale (MCS) was adopted to the assessment of comfort, together with the techniques of human backshape and EMG measures 相似文献
7.
对经口感染(4.0×10~5个/鸡)贝氏隐孢子虫(Cryptosporidium baileyi)卵囊的雏鸡在其饲料中分别添加交沙霉素(4.0g/kg)、复方新诺明(4.9g/kg)、大蒜素(400mgkg/)、甲硝唑(2.0g/ks),任鸡自由采食,连用7d.结果表明,实验鸡潜隐期延长,粪中卵囊数显著降低,体重明显增加,血液学值及血清酶学指标与健康对照鸡无明显差异.由此证明,这4种药物对实验性雏鸡隐孢子虫病有不同程度的预防作用. 相似文献
8.
龚宪章 《湖南农业大学学报(自然科学版)》1990,17(1)
闸述了L波段低噪声晶体管放大器中各种降低噪声措施的基本原理,给出了低噪声电路设计的一般原则,以及L波段卫星地面接收系统前端低噪声高频放大器输入级中噪声匹配电路的设计举例. 相似文献
9.
李梅林 《湖南农业大学学报(自然科学版)》1990,17(3)
本文以内燃机排气噪声必需的消声量频率特性作为设计消声器的依据,论述了扩张室式和穿孔板复合消声器的设计.试验表明,采用这种新型复合消声器后,S195柴油机排气噪声得到了有效控制. 相似文献
10.
羊源链球菌的分群鉴定及多价灭活苗的研制 总被引:1,自引:0,他引:1
2000~2003年,从安阳、邯郸等6个地区采取280份羊的病料,从中分离到150株链球菌,对其中20株典型分离株进行了生化分群鉴定及兰氏分群A~G诊断试剂鉴定.结果表明,6个地区发生的羊的链球菌病主要由C群兽疫链球菌(7/20),D群(8/20),E群(2/20)引起.其分布无明显地域性.选择C、D、E群中各1株为菌种,制备多价链球菌灭活苗,通过免疫试验,保护率达92%以上,免疫期半年以上,疫苗4℃保存期1年以上,证明该灭活苗安全性好,免疫效果明显. 相似文献