Effects of liver regeneration on the proliferation of rat fetal hepatocytes after intrasplenical transplantation     

YANG Ming-zhi  MIN Jun  CHEN Ji-sheng  ZHOU Xiao-dong  WEI Jing 《园艺学报》2002,18(6):687-689

AIM:To study the effect of environment of liver regeneration on the proliferation of rat fetal hepatocytes after intrasplenical transplantation. METHODS:Fetal hepatocytes isolated from 3-week SD rat fetuses bred were transplanted into the spleens of liver regeneration model rats with 70% partial hepatectomy. The cell cycle of the hepatocytes in the remnants liver was analyzed by flow cytometer and the density dimensions of the donor fetal hepatocytes in spleen were measured by image analysis system 7 and 30 days post-transplantation, respectively. RESULTS:Compared with the control group, the proportions of S and G₂ /M cells in the remnants liver were obviously decreased (P＜0.05), but the density dimensions of the donor fetal hepatocytes in spleen increased significantly (P＜0.05) in rats with hepatectomy 7 days post-transplantation. CONCLUSION:The environment of liver regeneration is propitious to the proliferation of fetal hepatocytes after transplantation into spleen.  相似文献   

谷氨酰胺对肉仔鸡脾组织结构发育的影响   总被引：2，自引：1，他引：2  

李升和  张承飞  吴亮  王珏  顾有方  许万祥  金光明 《畜牧与兽医》2004,36(10):5-8

160只 1日龄艾维茵肉仔鸡随机分成 4组 ,分别饲喂添加 0 % ,0 2 % ,0 4%和 0 8%谷氨酰胺的饲粮 2 8d。每周末每组取 6只鸡 ,颈静脉放血致死 ,取脾脏 ,Bouin液固定 ,制作石蜡切片 ,HE染色 ,光镜观察 ,显微摄影 ,研究基础日粮中添加谷氨酰胺对肉仔鸡脾组织结构的影响。结果显示 :添加谷氨酰胺的各试验组脾小结增多、增大 ;动脉周围淋巴鞘增厚 ;椭球增多、增大 ,细胞排列疏松 ,鞘毛细血管内皮细胞增高、增多 ;红髓比例降低 ,脾索内浆细胞和巨噬细胞增多。添加 0 8%谷氨酰胺的脾小结变化明显 ,添加 0 4%谷氨酰胺的动脉周围淋巴鞘和椭球变化明显。说明日粮中适量添加谷氨酰胺可促进脾内淋巴细胞的增殖与分化 ,从组织学角度证明 ,谷氨酰胺可促进脾的免疫应答能力 ,提高机体的免疫功能  相似文献   

台盼蓝活体染色法观察瓦氏黄颡鱼血脾屏障研究     

贺扬  戴旭平  樊威  王均  覃川杰  陈杰  曹心雨 《南方水产科学》2021,17(2):44-50

为探讨瓦氏黄颡鱼(Pelteobagrus vachelli)脾脏组织结构和血脾屏障位置,该研究对其脾脏进行了形态大小、组织学和活体染色观察.结果显示,瓦氏黄颡鱼的脾脏位于腹腔中部,呈暗红色,脾体指数为0.120％～0.273％;对健康脾脏制备石蜡切片,分别进行HE染色和改良James染色,显微镜观察发现其脾组织主要由...  相似文献   

蚯蚓提取液改善老龄小鼠胸腺脾组织结构的研究   总被引：3，自引：0，他引：3  

郑素玲 《安徽农业科学》2010,38(5):2588-2589

[目的]研究蚯蚓提取液对老龄小鼠胸腺、脾脏组织结构的改善作用。[方法]健康雄性昆明小鼠随机分3组,分别为对照组A、衰老模型组B、用药组C,每组10只。B、C组腹腔注射D-半乳糖50 mg/（k.gd）,连续8周,建立衰老模型组;第5周始C组灌胃蚯蚓提取液50 mg/（kg.d）,连续4周。试验结束对小鼠进行颈椎脱臼处死,应用分析天平称量小鼠胸腺、脾脏重量,并对胸腺、脾脏进行常规石蜡切片。观察3组小鼠间胸腺、脾脏组织结构的差异。[结果]蚯蚓提取液提高了C组小鼠胸腺指数和脾指数,组织切片观察显示,C组小鼠胸腺皮质/髓质比例增高。[结论]蚯蚓提取液具延缓小鼠胸腺、脾脏组织结构衰老的作用。  相似文献   

雷蘑胞外多糖抗肿瘤活性研究     

王永斌  王允祥 《中国林副特产》2006,(5):10-12

从雷蘑AS 5.105深层发酵的滤液中分离得到胞外粗多糖CGP,以KM系S180荷瘤小白鼠为实验模型,用免疫器官重量法,进行高、中、低剂量CGP的腹腔注射实验。结果表明,CGP具有较高的抑瘤活性,并与剂量呈显著相关性;高剂量组与对照组相比较,小白鼠胸腺指数和脾脏指数均有显著增加,P<0.05;与低剂量组相比较,脾脏指数有显著增加,P<0.05。表明CGP能明显提高小白鼠的免疫功能。  相似文献   

Serum vascular endothelial growth factor in dogs with haemangiosarcoma and haematoma     

Meike Frenz  Franz-Josef Kaup  Stephan Neumann 《Research in veterinary science》2014

Splenic haemangiosarcomas are frequently seen in dogs. Because of their bad prognosis differentiation from other benign splenic lesions are of prognostic importance.  相似文献   

实验性脾虚证与自由基代谢的相关性及四君子汤对其的调整作用   总被引：12，自引：2，他引：10  

魏彦明  李文广 《畜牧兽医学报》2002,33(1):100-104

分别用小承气汤和利血平复制大鼠脾虚证模型，通过检测血清和心、肝、脾、肾等组织中抗氧化酶SOD和GSHP－Px的活性及脂质过氧化反应终产物MDA含量，研究脾虚证与自由基代谢的相关性及四君子汤对其影响。结果表明：两种方法复制脾虚证模型与对照组比较均表现为SOD活性显著升高（P＜0．05），GSH－Px活性显著降低（P＜0．05）；四君子汤反证治疗组与脾虚证组比较表现为SOD活性显著下降（P＜0．05），GSH－Px活性显著升高（P＜0．05），MDA含量显著降低（P＜0．05），与正常对照组接近。表明大鼠虚证动物模型与体内自由基代谢紊乱，脂质过氧化作用增强存在一定关系，健脾益气方药四君子汤能够提高体内抗氧化酶活性，拮抗自由基对生物膜的过氧化损伤，具有抗脂质过氧化损伤的作用。  相似文献   

Ultrasonography of the spleen in 50 healthy cows     

Braun U  Sicher D 《Veterinary journal (London, England : 1997)》2006,171(3):513-518

This paper describes the ultrasonographic appearance, location and size of the spleen in 50 healthy commercial milk cows destined for slaughter. The intercostal spaces of the left thoracic wall were scanned with a 3.5 MHz linear transducer. In each intercostal space, the appearance of the splenic parenchyma, the dorsal and ventral margins and the distance between them, and the diameter of the splenic vessels were recorded. The spleen was seen in intercostal spaces 7-12. It was 2.0-5.0 cm thick, and tapered ventrally. The splenic capsule appeared as an echogenic line. The splenic parenchyma consisted of numerous small regularly spaced echoes, and vessels within the parenchyma appeared as anechoic round to oval or elongated images. The long axis was oblique, running caudodorsal to cranioventral. The distance from the dorsal margin of the spleen to the midline of the back was greatest in the 7th intercostal space (60.9+/-6.81) and smallest in the 12th intercostal space (12.7+/-2.85 cm). The extent of the spleen was greatest in the 8th intercostal space (24.9+/-10.77 cm) and smallest in the 12th intercostal space (9.5+/-5.38 cm). The mean diameter of the splenic vessels ranged from 0.66+/-0.28 to 0.90+/-0.65 cm, depending on the intercostal space scanned. Ultrasonography of the spleen in healthy cows provides information that can be used as a reference when examining cattle with suspected splenic disease.  相似文献   

Contrast harmonic imaging characterization of canine splenic lesions     

Ohlerth S  Dennler M  Rüefli E  Hauser B  Poirier V  Siebeck N  Roos M  Kaser-Hotz B 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2008,22(5):1095-1102

Background: Although B-mode ultrasound is very sensitive for the detection of splenic lesions, its specificity is low. Contrast harmonic imaging is used successfully to differentiate benign from malignant liver lesions in humans and dogs.
Hypothesis: Contrast harmonic imaging could be useful to differentiate benign and malignant splenic lesions in dogs.
Animals: Sixty dogs (clinical patients) with splenic abnormalities detected during abdominal ultrasonography.
Methods: A prospective study was performed with a Philips ATL 5000 unit for contrast pulse inversion harmonic imaging (mechanical index: 0.08, contrast medium: SonoVue). Perfusion was assessed subjectively and quantitatively.
Results: Cytology or histology identified 27 benign (hyperplasia, extramedullary hematopoiesis, hematoma) and 29 malignant (hemangiosarcoma, malignant lymphoma, malignant histiocytosis, mesenchymal tumors without classification, mast cell tumors, and others) lesions and 4 normal spleens. Except for 1 benign nodule, extensive to moderate hypoechogenicity was only seen in malignant lesions during wash-in, at peak enhancement, and during wash-out ( P = .0001, odds ratios: 37.9 [95% CI 4.5–316.5], 66.4 [95% CI 8.0–551.1], and 36.9 [95% CI 4.4–308.4]). Although all but 1 benign lesion enhanced well and were mildly hypo-, iso-, or hyperechoic in comparison with the normal spleen during all blood pool phases, marked enhancement occurred both in benign as well as in malignant splenic lesions. Quantitative perfusion values did not differ significantly between benign and malignant lesions.
Conclusions and Clinical Importance: Moderate to extensive hypoechogenicity clearly identifies canine splenic malignant lesions. In nodules with marked enhancement, contrast harmonic ultrasound is of limited value and histology is needed.  相似文献   

Mesenteric lymph drainage alleviates spleen injury in hemorrhagic shock rats     

LIU Hua  XING Li-qiang  ZHAO Zi-gang  NIU Chun-yu 《园艺学报》2013,29(8):1496-1501

AIM：To observe the effects of post-shock mesenteric lymph (PSML) drainage on histopathology, apoptosis, cell cycle and proliferation of the spleen in rats with hemorrhagic shock. METHODS：Eighteen Wistar rats were randomly divided into sham, shock and shock+drainage groups (n=6 in each group). The hemorrhagic shock model was established in the shock and shock+drainage groups. Fluid resuscitation for 30 min was performed 1.5 h after hypotension, and PSML was drained in the rats in shock+drainage group from 1 h after hypotension to 3 h after resuscitation finished. The fixed spleen tissue was harvested from each rat for histological observation with HE staining. The apoptosis of splenocytes was observed by Hoechst 33258 staining. The expression of Bcl-2 and Bax proteins was detected by immunohistochemical staining. The cell cycle and the expression of p53 protein were measured by flow cytometry, and the proliferation index (PI) was calculated. RESULTS：Compared with sham group, splenic tissue injury appeared in the shocked rats. The apoptotic cells and the expression of Bax and p53 in shock group were increased, while Bcl-2 expression was decreased. The percentage of G2/M cells in shock group was decreased. Compared with shock group, the splenic tissue damage in shock+drainage group was significantly attenuated. Moreover, the number of apoptotic cells, the percentage of G0/G1 cells, and the expression of Bax and p53 were obviously decreased, and the G2/M cells, Bcl-2 protein expression and PI were significantly increased in shock+drainage group. CONCLUSION: PSML drainage alleviates splenic injury in hemorrhagic shock rats, which may be related to reducing the apoptosis of splenocytes.  相似文献