Inhibitory effects of LDL-ACM complex on subcutaneous implanted tumors in nude mice()     

JIANG An-li  KANG Lu-dong  ZHAO Chun-hua  BI Wen-xiang  HU Xiao-yan  KONG Feng  XU Song-de 《园艺学报》2003,19(1):55-58

AIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor.  相似文献   

Effect of lansoprazole on gastric ulceration in rats     

XU Hua  YU Li-ping  WEN Jie  ZHAO Wen  HUANG Ya-dong  LI Xiao-kun 《园艺学报》2002,18(6):698-700

AIM:To investigate the effect of lansoprazole on gastric ulceration in rats. METHODS:Using the gastric ulcer model induced by hemorrhagic shock, restraint water-immersion stress and pylorus-ligature, the protective effect of lansoprazole (iv) on gastric ulceration was observed. RESULTS:Pretreatment with lansoprazole (7.5-60 mg/kg) significantly inhibited the formation of gastric ulcer in the three models in a dose-dependent manner. The autiulcer efficacy of lansoprazole was similar to that of omeprazole in the equal dose, but stronger than that of omeprazole for ulcer induced by water-immersion stress.CONCLUSION:The intravenously administered lansoprazole inhibited formation of experimental gastric ulcer in rats.  相似文献   

一例母犬胃内异物合并绝育手术的诊疗     

张小苗  周玉照 《现代畜牧兽医》2021,(1)

文章研究治愈母犬胃内异物的同时做绝育手术的效果。对病犬采用临床检查、血常规检查、血清生化检查、影像学检查等方法确诊胃内有异物,并通过手术取出胃内异物的同时进行绝育。结果表明:从母犬胃内取出一块大小为2 cm×1 cm×1 cm的石子,并成功给母犬做绝育,术后恢复效果较好。该病例的诊疗经验为临床上犬胃内异物的诊治和绝育手术提供参考。  相似文献   

ER-α36 and miR-143 involved in invasion of gastric cancer cells     

ZOU Feng  WANG Xu-ming  LIU Li-jiang 《园艺学报》2012,28(12):2167-2171

AIM: To investigate the relationship between the expression of estrogen receptor α36 (ER-α36) and the invasion of gastric cancer cells. METHODS: SGC7901 cells were treated with 17β-estradiol at high or low concentration. The invasion of gastric cancer cells and the expression of ER-α36 were detected. The SGC7901 cells with the characteristics of stable low expression and high expression of ER-α36 were constructed. The invasion ability and microRNA sequences were determined in above-mentioned recombinant cells. RESULTS: The decreased invasion ability and ER-α36 expression were detected in SGC7901 cells treated with low concentration of 17β-estradiol. The situation was the opposite in the cells treated with high concentration of 17β-estradiol. The expression of miR-143 was significantly decreased in the SGC7901 cells with stable high expression of ER-α36 and was increased in the SGC7901 cells with stable low expression of ER-α36. CONCLUSION: The expression of ER-α36 is positively related to the invasion of gastric cancer cells. It is possible that miR-143 plays an important role in the regulation of gastric cancer invasion.  相似文献   

The anticancer activity of genistein on implanted tumor of human primary gastric carcinoma cells in nude mice     

ZHOU Hai-bo  CAI Jian-ting  DU Qin  CHEN Jin-ming△ 《园艺学报》2007,23(12):2379-2384

AIM: To investigate the apoptosis of implanted tumor of primary human gastric cancer cells in nude mice induced by genistein and the relation between this apoptosis and expression of bcl-2 and bax.METHODS: Establishing a transplanted tumor model by injecting human primary gastric cancer cells into subcutaneous tissue of nude mice.The different doses of genistein (0.5mg/kg,1mg/kg and 1.5 mg/kg ) were directly injected beside tumor body respectively,for six times at an interval of two days.Then changes of tumor volume were measured continuously and tumor inhibition rate of each group was calculated.We observed the morphologic alteration by electron microscope,measured the apoptotic rate by TUNEL staining method,detected the expression of apoptosis-regulated gene bcl-2 and bax by immunohistochemical staining and RT-PCR.RESULTS: Genistein could significantly inhibit carcinoma growth when it was injected near the carcinoma.Genistein induced implanted tumors cells to undergo apoptosis with apoptotic characteristics by transmission electron microscope.The apoptosis index of above three groups was increased progressively.Positive rate of Bcl-2 protein of above three groups was decreased progressively and positive rate of Bax protein of above three groups was increased progressively by immunohistochemical staining.The density of bcl-2 mRNA decreased progressively and the density of bax mRNA increased progressively with elongation of time by RT-PCR.CONCLUSION: Genistein is able to induce the apoptosis of transplanted tumor cells.This apoptosis may be mediated by down-regulating bcl-2 and up-regulating bax mRNA and its protein.  相似文献   

The anti VEGF monoclonal antibody conjugated with nanometer particle of 5-FU     

HUANG Kai-hong  WANG Ling-yun  ZHAO Xiao-long  LIU Jian- hua  CHEN Ru-fu  ZHU Zhao-hua 《园艺学报》2007,23(2):258-261

AIM:The characteristics of nanometer particles, which were prepared by the conjugation of anti-VEGF monoclonal antibodies and 5-fluorouracil-loaded polylactic acid nanometer particles (5-FU-NPs), were investigated for improving the anticancer activity of 5-FU. METHODS:The method of couple linkage of chemical bonds was used to prepare the 5-FU-NPs with VEGF antibody, then the appearance, distribution of particle diameter, releasing in vitro and the immunological activity were detected. RESULTS:The 5-FU-Ab-NPs appeared as regular globular, the average particle diameter was (202±23)nm. The 5-FU-Ab-NPs possessed the similar delayed release character of 5-FUs. More than 80% of the immmunogical activity were detected in conjugates-retained antibody by the immunological methods and electron microscopy. CONCLUSION:The 5-FU-Ab-NPs possess the similar delayed released activity to 5-FU-NPs and have double activity of immune targeting and delayed releasing, which may increase the local concentration of 5-FU.  相似文献   

European College of Equine Internal Medicine Consensus Statement—Equine Gastric Ulcer Syndrome in Adult Horses          下载免费PDF全文

B.W. Sykes  M. Hewetson  R.J. Hepburn  N. Luthersson  Y. Tamzali 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(5):1288-1299

  相似文献   

6种大型海洋掠食性鱼类胃含物角质颚分类研究     

欧利国  顾心雨  王冰妍  刘必林 《渔业科学进展》2022,43(4):105-115

为了研究大型海洋掠食性鱼类胃含物中角质颚的分类效果,于2017年10月,2018年3、5和11月,收集了南大西洋、中东太平洋和西印度洋海域的大型海洋掠食性鱼类的36个胃并分析其残留角质颚的形态。对采集的角质颚形态侧视图和顶视图进行种类鉴定,建立检索表,提取椭圆傅里叶描述子(EFDs)系数进行聚类分析。结果显示,通过对胃含物角质颚形态进行种类鉴定,共发现头足类2目10科17种。根据角质颚形态分类检索分析,17种头足类角质颚形态种间差异明显。枪形目(Teuthoidea)的13种头足类角质颚喙部形态分别为三角形和等腰三角形,侧壁为近似菱形,翼部发达;八腕目(Octopoda)的4种头足类角质颚有喙部发育,侧壁近似长四边形,有翼部发育。角质颚形态信息聚类分析在目级别区分效果显著。通过对胃含物角质颚形态进行分类研究,不仅可佐证胃含物头足类的识别,而且丰富了胃含物头足类的鉴定内容,也为大型海洋掠食性鱼类胃含物头足类分类鉴定体系奠定基础。  相似文献   

Purification and characterization of stomach protease from the turbot (Scophthalmus maximus L.)     

Hai-ying Wang  Yue-jun Wang  Qing-yin Wang  Chang-hu Xue  Mi Sun 《Fish physiology and biochemistry》2006,32(2):179-188

Proteolytic activity in the different parts of the digestive tract of the turbot (Scophthalmus maximus L.) were studied in this work. One pure protease was isolated from turbot stomach and its behavior was studied. Results showed the optimum pH for proteases in the different parts of the digestive tract of the turbot were pH 2.0 for the stomach, pH 8.0 for the pylorus cecum, pH 8.0 for the foregut, pH 8.5 for the midgut, and pH 8.0 for the hindgut. The activity of proteases in the different parts of the digestive tract were in the sequence pylorus cecum protease > stomach protease > foregut protease > midgut protease > hindgut protease. The stomach protease was purified by ammonium sulfate precipitation and column chromatography on DEAE-Sepharose F.F. and Sephadex G-100. The purified enzyme gave a single band in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Its molecular weight was found to be approximately 42,000 Da. The enzyme is stable at pH 1.0–9.0 and at temperatures below 40°C. Its activity was maximum at pH 2.0 and 40°C. When reaction time was prolonged the optimum temperature of the enzyme tended to decline. The enzyme was activated by Mn²⁺ and Cu²⁺ and inactivated by Fe³⁺. It was fully inhibited by pepstatin and partially inhibited by PMSF, TPCK, PCMB, and NBS. These results imply the enzyme is a pepsin.  相似文献   

Effect of captopril on AGS nude mouse model of gastric cancer     

LI Li  JIN Zhen-dong  CAI Min  WANG Bin  CHENG Feng-tao 《园艺学报》2014,30(4):635-639

AIM: To observe the effect of captopril on the genesis and development of gastric cancer, and to explore its clinical treatment feasibility for gastric cancer. METHODS: The human gastric cancer cell line AGS was used to establish a tumor model in nude mice, and the model mice were randomly divided into 3 groups: positive control (5-fluorouracil) group, normal control (saline) group and experimental (captopril) group. After intraperitoneal injection or intragastric administration of the drugs, the tumor growth curve was determined, and the tumor tissues were also sampled to detect the expression of Ki-67, STAT3, Bax and Bcl-2 by real-time quantitative PCR and immunohistochemistry. The apoptosis was detected by TUNEL+DAPI staining. RESULTS: The tumor growth curve showed that the tumor model in the nude mice was successfully established. The tumor volumes among groups showed significantly different after 14 d growth. The increase in the tumor volume in normal control group was significantly faster than that in the other two groups, and that in positive control group was the slowest. The expression of Bax in captopril group increased, and the expression of STAT3, Ki-67 and Bcl-2 was reduced as compared with normal control group and positive control group. Compared with normal control group, the apoptotic rate increased significantly, and the protein expression of p-STAT3 and STAT3 decreased obviously in positive control group and captopril group. CONCLUSION: With better feasibility, angiotensin-converting enzyme inhibitor captopril has a significant effect on treating gastric cancer in the AGS nude mouse model by regulating the expression of STAT3, Bax, Bcl-2 and Ki-67 to accelerate the apoptosis of cancer cells, thus inhibiting tumor growth.  相似文献